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P Oeding

Publications and source records attributed to P Oeding.

At least 37 records · Page 2Linked to original sources
PubMed1983

Comparison of four methods for differentiation of Staphylococcus aureus from other Micrococcaceae in the routine laboratory.

Four methods for the identification of Staphylococcus aureus (tube coagulase test, thermostable nuclease test, indirect agglutination of fibrinogen coated erythrocytes and a commercial latex kit: SeroSTAT Staphylococcus Test) have been compared. Clinical isolates (698) and 40 reference strains of Micrococcaceae were included in the study together with control organisms. The coagulase test gave no false positive results but 39/406 clinical isolates of S. aureus were negative at 2h and one half were only weakly positive. At 18 h, all but 2 of 406 isolates gave a positive reaction. The thermostable nuclease test was very specific; no clinical isolates of S. aureus gave negative results and no "coagulase-negative" clinical isolates gave a definite positive reaction. The indirect haemagglutination method was sometimes difficult to interpret and frequently gave negative or doubtful results for S. aureus. The SeroSTAT test was easy to use and interpret and was specific; the method is suitable for routine laboratory use, particularly when a rapid result is desirable.

Bacteriological Techniques↗
PubMed1981

Detection of Yersinia enterocolitica O-antigens by double diffusion in agar and chemical characterization of lipopolysaccharides.

Double diffusion in agar showed that yersinia enterocolitica O-serotypes 3, 7, 8 and 9 each contained a type-specific precipitinogen. Serotype 6 contained several specific precipitinogens and was heterogeneous. The type-specific precipitinogens were shown to be lipopolysaccharides (LPS). Acid hydrolysis of the isolated LPS followed by gel filtration separated the material into two peaks, the first of which contained the serologically active fractions. The active fraction of serotype 3 contained relatively large amounts of a terminally linked deoxyhexose, probably 6-deoxy-L-altrose. Fucose was found to be present in serotype 7.

Antigens, Bacterial↗
PubMed1980

Immunochemical analysis of the teichoic acid from Staphylococcus simulans.

The wall teichoic acid of Staphylococcus simulans has been characterized as a glycerol phosphate polymer with glycosidically linked N-acetylglucosamine. Susceptibility to beta-N-acetylglucosaminidase and serological similarity to poly C beta from Staphylococcus saprophyticus, showed that the amino sugar is in the beta-configuration.

Immunologic Techniques↗
PubMed1979

Immunochemical analysis of the teichoic acid from Staphylococcus hyicus.

The wall teichoic acid of Staphylococcus hyicus has been isolated and characterized. The teichoic acid is a glycerol phosphate polymer with glycosidically linked N-acetylglucosamine. Interaction with concanavalin A and susceptibility to alpha- but not to beta-N-acetylglucosaminidase showed that the sugar is in the alpha-configuration.

Acetylglucosaminidase↗
PubMed1978

Immunochemical studies on the specific agglutinogens of Staphylococcus aureus. I. Isolation and characterization of antigen h1.

The specific Staphylococcus aureus agglutinogen h1 has been purified and shown to be a protein with a molecular weight of about 95,000. Chemical analysis revealed all the common amino acids, except tyrosine and the sulphur-containing ones. The purified h1 antigen was strongly immunogenic in rabbits. The antiserum produced one precipitation line on double diffusion in agar against a suspension of bacteria. It also agglutinated bacteria of the h1-containing strains, as well as tanned sheep erythrocytes sensitized with h1, in high dilutions. Antibodies to other known staphylococcal antigens were not detected. The identity between h1 and Pillet's type 9 antigen was confirmed.

Agglutination↗
PubMed1977

Classification of coagulase-negative staphylococci in the diagnostic laboratory.

One hundred and ninety-eight coagulase-negative staphylococci isolated from urines, blood cultures, and pus samples were classified by means of two identification schemes, and their wall teichoic acids were determined serologically. S. epidermidis, S. saprophyticus, and S. cohnii were identified reliably by the use of five criteria: acid aerobically from sucrose, trehalose, and mannitol, phosphatase production, and sensitivity to novobiocin. Further species, notably S. haemolyticus and S. hominis, could be identified when haemolysis on blood agar plates was included in the criteria group. The investigation shows, that a considerable number of coagulase-negative staphylococci isolated from human specimens belong to species other than S. epidermidis and S. saprophyticus. These staphylococci can cause human infections and should be identified in the diagnostic laboratory. S. epidermidis and S. saprophyticus were found to contain the teichoic acids previously identified in these species. S. cohnii contained the same teichoic acids as S. saprophyticus. No characteristic teichoic acid was demonstrated in the other species, but several strains contained poly C (beta-N-acetylglucosaminylglycerol teichoic acid).

Bacteriological Techniques↗
PubMed1976

A comparison of antigenic structure and phage pattern with biochemical properties of Staphylococcus aureus strains isolated from sheep.

Of 84 Staphylococcus aureus strains isolated from the anterior nares of healthy sheep and from the udders of ewes suffering from purulent mastitis the 89 per cent belonging to the C biotype contained agglutinogen h2 as well as polysaccharide Abeta. Ninety-five per cent of the C biotype strains were lysed by the bovine phage 78, the human phage set giving only weak reactions at RTD X 100. Two pigment-negative deficit variants as well as three unclassified strains gave similar results while three A biotype strains and one E biotype strain were definitely different. The close correlation between biochemistry, serology and phage typing substantiates the practical usefulness of the subdivision of S. aureus into biotypes.

Agglutinins↗
PubMed1976

Characteristics of Staphylococcus aureus strains isolated from different animal species.

Staphylococcus aureus strains originating from humans, cows, poultry, pigs, dogs and pigeons were characterised according to the biotyping scheme of Hájek and Marsálek (1971). All strains obtained from poultry, dogs and pigeons and the majority of bovine, human and porcine strains were classifiable as belonging to different biotypes. Two types were found to be present among poultry strains isolated in Europe and Japan. The porcine strains formed a heterogenic collection. One biotype predominated in the other host species. The characteristic S aureus wall teichoic acid (beta-N-acetylglucosaminyl ribitol teichoic acid) was present in nearly all poultry and pig strains. Strains from dogs and pigeons were found to present several properties which were not in agreement with the species description given for S aureus. They did not produce acetoin from glucose and their capacity to produce acid from mannitol in anaerobic conditions was very weak or absent. They were often negative in the clumping factor (slide coagulase) test and usually did not produce hyaluronidase. The production of acid from glucose in anaerobic conditions was slower and less intensive in these strains than in the S aureus strains from other origins. The results of this study support the concept of subdividing the species S aureus into biotypes or ecotypes.

Animals↗
PubMed1975

Studies on polysaccharide C of Staphylococcus epidermidis. 1. Isolation and chemical characterization.

Polysaccharide C (poly C) has been isolated from two strains of S. epidermidis and characterized chemically. The results suggest that poly C is a wall N-acetylglucosaminylglycerol teichoic acid, linked through 1:3-phosphodiester linkages. One poly C preparation contained only beta-linked N-acetyglucosamine, the other traces of alpha-linked sugar in addition. The degree of substitution of sugar in the poly C preparations from the two strains was about 50 and 25 per cent.

Acetylglucosamine↗
PubMed1975

Studies on polysaccharide C of staphylococcus epidermidis. 2. Antigenic properties.

The antigenic properties of polysaccharide C (poly C) of S. epidermidis strongly support the analytical indications that it is an N-acetylglucosaminylglycerol teichoic acid with 1:3-phosphodiester linkages. The major antigenic determinant is N-acetyglucosamine, predominantly present in the beta-configuration. Also anti-glycerophosphate antibodies are produced, apparently dependent on the degree of glycosylation. Purified poly C was unable to sensitize either normal or tanned sheep erythrocytes for agglutination in antisera.

Acetylglucosamine↗
PubMed1975

Characterization of Micrococcaceae from the urinary tract.

Two hundred and seven urinary strains of staphylococci and micrococci were classified biochemically according to Baird-Parker (1963) and by means of a simplified schema. One hundred and thirteen strains belonged to Staphylococcus aureus, S. epidermids or S. saprophyticus (64 strains) according to the simplified schema, respectively to Baird-Parker's sub-groups SI, SII or M3. S. saprophyticus was isolated from young, female out-patients, was relatively resistant to novobiocin and contained poly AbetaC (beta-N-acetylglucosaminyl ribitol teichoic acid and beta-N-acetylglucosaminyl glycerol teichoic acid). S. aureus and S. epidermidis were isolated from older, male in-patients, were sensitive to novobiocin and contained poly A (N-acetylglucosaminyl ribitol teichoic acid), respectively poly B (glucosyl glycerol teichoic acid). Ninety-four strains belonging to other Staphylococcus or Micrococcus subgroups could not be classified by the simplified schema. With few exceptions, these strains were sensitive to novobiocin and either gave a precipitin reaction corresponding to poly C or were non-typable with the teichoic acid reference systems used. The simplified schema is recommended for the classification of coagulase-negative strains of Micrococcaceae.

Acetylglucosamine↗