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P Pei

Publications and source records attributed to P Pei.

At least 19 recordsLinked to original sources
PubMed2003

Differential use of cardiac troponin T versus I in hemodialysis patients.

BACKGROUND: Cardiac troponin T (cTnT) is frequently elevated in asymptomatic hemodialysis (HD) patients and predicts increased cardiovascular morbidity and mortality. Compared to cTnT, cardiac troponin I (cTnI) has a shorter half-life. How this influences its diagnostic reliability in chronic HD patients is only partially known. PATIENTS AND METHODS: First, in a cross-sectional study cardiac troponins were measured in 31 asymptomatic HD patients. A third-generation cTnT assay was used. The rate of false positive tests and the intraindividual variability were determined. Second, in a retrospective analysis over 12 months all acute events with clinical suspicion for acute coronary syndrome (ACS) were analysed in the same patients to determine the diagnostic power of cTnT by receiver-operating curve (ROC) plot. RESULTS: Cross-sectional study: 9 of 52 (17%) cTnT and 0/52 cTnI (0%) tests were positive in asymptomatic HD patients with a low intraindividual variability. Retrospective analysis: 16 acute clinical events with determination of cTnT were recorded, and in 4/16 an ACS was diagnosed. Using a cut-off level of 0.1 microg/l, the cTnT test reached a sensitivity of 100%, a specificity of 42%, a positive predicitive value of 36% and a negative predictive value of 100%, using a cut-off level of 0.2 microg/l the corresponding values were 75%, 58%, 38% and 88%. CONCLUSIONS: Cardiac TnT, but only rarely cTnI, is elevated in a significant number of asymptomatic HD patients. For diagnosis of ACS in HD patients, a combination of cTnT and cTnI may be used, since the former has higher sensitivity and the latter higher specificity. A higher threshold value for cTnT in HD patients could further increase its diagnostic accuracy.

Adult↗
PubMed2000

[Water births and the exposure to HIV].

The risk of a work related HIV-exposure or infection to midwives, or other HCW, in attending waterbirths of (possible unrecognized) HIV-positive women is unknown. Our goal was the quantification of the loss of blood of the childbearing woman after a waterbirth into the waterpool, in taking pool-water-samples of 14 different waterbirths and measuring the hemoglobin in the plasma, and then correlating the mean found loss of blood and the mean quantity of poolwater with a hypothetical HIV-RNA viral load of 10(3)-10(6) copies per milliliters (ml) blood. All attended waterbirths were evaluated with a questionnaire regarding: parity of the child-bearing woman; serostatus for HIV/Hepatitis-B (HBV) and Hepatitis-C (HCV); length of the birth-process; perineum-rupture or not, etc. Questions concerning the HCW in implementing universal precautions like: type of gloves and garment used, if at all; duration of water contact with the hands; existing skin lesions; HBV-vaccination-status; years of professional experiences as a midwife; how many waterbirths attended etc. were also evaluated. The mean calculated loss of blood into the pool was 300 ml, the mean pool-water content 633 liters. With a hypothetical (maximal) HIV-RNA viral load of 10(6) copies per ml blood, we calculated a mean HIV-RNA viral load of 476 copies per ml pool-water. We also found 37% of the interviewed midwives (n = 14) to have skin lesions on hands or fingers; 1 received splashes into her (unprotected) face and 1 was not immunized against HBV. The mean loss of blood of 300 ml into the pool is a relevant amount. The skin-contact of the HCW with the potentially contaminated water is the norm, because of the failure of the type of used gloves. Because of the diluting effect of the poolwater, we estimate the potential risk for a HIV-exposure to intact skin as minimal and, therefore a potential HIV-infection as "low level" and to be unlikely. However, a risk for nosocomial HBV-infection is significantly higher. We recommend wearing long-sleeved gloves, waterproofed garment, and HBV-vaccination to all HCW.

Baths↗
PubMed2000

Sustained angiogenesis enables in vivo transplantation of mucocutaneous derived AIDS-related Kaposi's sarcoma cells in murine hosts.

AIDS-related Kaposi's sarcoma (AIDS-KS), the most prevalent HIV-associated malignancy, is a debilitating, potentially fatal disease. Currently, there is a need for development of AIDS-KS therapies that are not only well tolerated, but also capable of providing sustained remission. Preclinical assessment of pharmacological parameters and therapeutic efficacies are dependent upon in vivo parameters. However, there are currently no animal KS models and mucocutaneous KS cell isolates have proved to be non-tumorigenic in animal hosts. This report describes the development of a murine model that enables in vivo transplantation of 'native' low population doubling level AIDS-KS cells from biopsy-confirmed mucocutaneous lesions. The angiogenic phenotype of in situ AIDS-KS lesions is reconstituted via controlled release of a complete angiogenic peptide, recombinant human basic fibroblast growth factor (bFGF), from locally injectable, biodegradable polylactide-co-glycolide implants. Consequential to the sustained local release of bioactive bFGF, a murine vascular network is established, which facilitates the in vivo transplantation of AIDS-KS cells. Desirable aspects of this model include: low cost murine species, transplantation of non-selected patient cells and use of animal hosts that are T cell-deficient. The transplanted human AIDS-KS cells and extensive murine vascular network create lesions that retain a striking resemblance, at both the gross and microscopic levels, to in situ AIDS-KS tumors. Because the bFGF-induced murine vascular network is analogous to the abundant vascularity present in AIDS-KS lesions, this murine model should provide an excellent vehicle for numerous clinically relevant studies, such as assessment of drug clearance at AIDS-KS lesional sites. Finally, applicability of this method is not restricted to AIDS-related malignancies. Establishment and maintenance of an extensive host vascular network should augment success rates for in vivo transplantation of numerous other human cell strains or lines.

Acquired Immunodeficiency Syndrome↗
PubMed1999

Thiol redox modulation of doxorubicin mediated cytotoxicity in cultured AIDS-related Kaposi's sarcoma cells.

The chemotherapeutic, doxorubicin, is currently used empirically in the treatment of AIDS- related Kaposi's sarcoma (AIDS-KS). Although often employed in a chemotherapeutic cocktail (doxorubicin, bleomycin, vincristine) single-agent therapy has recently been attempted with liposome encapsulated doxorubicin. Although doxorubicin's mechanism of action against AIDS-KS is unknown, we hypothesized that doxorubicin's ability to undergo redox cycling is associated with its clinical efficacy. The current study was conducted to investigate the effects of doxorubicin on selected xenobiotic-associated biochemical responses of three cellular populations: KS lesional cells, nonlesional cells from the KS donors, and fibroblasts obtained from HIV- aged matched men. Our results show that during doxorubicin challenge, there are strong positive correlations between cellular glutathione (GSH) levels and viability (r = 0.94), NADPH levels and viability (r = 0.93), and GSH and NADPH levels (r = 0.93), and demonstrate that as a consequence of their abilities to maintain cellular thiol redox pools HIV- donor cells are significantly less susceptible to doxorubicin's cytotoxic effects relative to AIDS-KS cells. Additional studies further supported the contribution of reduced thiols in mediating doxorubicin tolerance. While pretreatment with the GSH precursor, N-acetylcysteine was cytoprotective for all cell groups during doxorubicin challenge, GSH depletion markedly enhanced doxorubicin's cytotoxic effects. Studies to investigate the effects of a hydroxyl scavenger and iron chelator during doxorubicin challenge showed moderate cytoprotection in the AIDS-KS cells but deleterious effects in the HIV control cells. Inactivation of the longer lived membrane generated ROI in the cytoprotective deficient AIDS-KS cells, as well as an impairment of endogenous defenses in the HIV- donor control cells, may account for these scavenger and chelator associated findings. In summary, our findings show that doxorubicin mediates, at least in part, its AIDS-KS cellular cytotoxic effects by a redox related mechanism, and provides a biochemical rationale for doxorubicin's clinical efficacy in AIDS-KS treatment.

AIDS-Related Opportunistic Infections↗
PubMed1998

Free/total prostate-specific antigen ratio can prevent unnecessary prostate biopsies.

OBJECTIVES: To evaluate the ability of free/total prostate-specific antigen (PSA) ratio to improve specificity of prostate cancer detection, compare Diagnostic Products Corporation (DPC) Immulite and Ciba Corning ACS 180 total (t)PSA assay, and define an assay-specific cutoff point and reflex range for DPC PSA ratio (PSAR). METHODS: In a prospective study, 206 men were enrolled with measurement of both assays. Group 1 consisted of 173 men with a suspicion of prostate cancer (PCA). Thirteen men with known PCA (group 2) and 20 men younger than 32 years (group 3) were used as control groups. RESULTS: Our results in group 1 (115 with benign prostatic hyperplasia [BPH], 58 with PCA) revealed a sensitivity of 82.7%, a specificity of 45.2%, and an accuracy of 57.8% for the DPC tPSA assay (cutoff point more than 4.0 ng/mL) within the entire PSA range. tPSA values of the ACS 180 assay were 1.97-fold higher. Within the tPSA gray zone of 2.5 to 10 ng/mL (66 BPH, 23 PCA), specificity and accuracy of DPC tPSA can be improved by using the DPC PSAR (cutoff point less than 19%) from 33.3% to 71.2% and 42.7% to 70.8%, respectively, maintaining the same sensitivity level of 69.6%. CONCLUSIONS: By combining tPSA testing with PSAR within the gray zone, 39.7% (25 of 63) of unnecessary biopsies can be saved, without missing any additional cancers compared with tPSA testing alone. The optimal reflex range for DPC PSAR is 2.5 to 10 ng/mL and the best PSAR cutoff point for biopsy criterion is less than 19% in our high-risk population, with a cancer yield of 34%. Because we still do not have an international PSA standard, it is important to use assay-specific "normal values" and PSAR cutoff points.

Aged↗
PubMed1997

[The effect of short-term hyperventilation on the concentration of ionized serum calcium].

BACKGROUND AND OBJECTIVE: Paraesthesias and carpopedal spasms on hyperventilation are explained by a reduction in ionised serum calcium (ISC). We tested whether 5-minute hyperventilation changes the concentration of ISC. SUBJECTS AND METHOD: Arterial blood samples were obtained via a small plastic catheter introduced into the femoral artery of ten healthy male volunteers (mean age 33 years) before, during and after 5 minutes of hyperventilation, which was achieved by deep and rapid breathing and considered adequate when the end-expiratory pCO2 had fallen to 2.5 kPA within the first minute and remained below this level during the remaining 4 minutes. These criteria were met in nine of the ten patients. The ISC concentration was measured with an ion-selective electrode, the pH, paCO2 and bicarbonate levels with an autoanalyser. RESULTS: Paraesthesias of the fingers and hand occurred in nine of the volunteers, carpopedal spasms in seven. Despite a definite rise in pH from 7.39 +/- 0.02 to 7.75 +/- 0.045, the concentration of ISC did not change significantly during the hyperventilation. CONCLUSION: Paraesthesias and carpopedal spasms which occur during hyperventilation are not caused by a fall in ionised serum calcium in arterial blood.

Adult↗
PubMed1996

[Thrombosis-resistant heparin-coated diffusion membrane oxygenators: an experimental study].

In the present study the thromboresistance of heparin-coated diffusion membrane oxygenators (Jostra, M 30) combined with heparin-coated venous reservoirs, tubing sets and arterial filters was investigated in six bovine experiments (70 +/- 5 kg). The perfusion with reduced systemic heparin dose (100 IE/kg) body weight) was performed with activated clotting time over 180 seconds. The perfusion began with a blood flow of 31/min and was maintained during six hours. Clotting studies including blood platelet count, activated clotting time, fibrinogen (factor I), antithrombin III and fibrinopeptid A were performed before the operation and ten minutes, two hours and five and six hours after beginning of bypass. The venous and arterial saturation remained stable during the whole investigation. After ten minutes activated clotting time dropped from 619 +/- 114s to 203 +/- 15s after six hours (p < 0.05). The antithrombin III level changed significantly from 109 +/- 11% to 95 +/- 16%. Factor 1 and fibrinopeptid A changes were not significant: from 1.6 +/- 0.3 g/1 to 1.5 +/- 0.3 g/1, and 3.0 +/- 1.4 ng/mL to 3.5 +/- 1.2 ng/mL, accordingly. There were no mechanical defects and especially no plasma leakage. Slight sediments were found only in areas of stagnant blood flow. The investigated bypass circuit with reduced systemic heparinization seems therefore particularly appropriate for long-term perfusions.

Animals↗
PubMed1995

Normothermia versus hypothermia during cardiopulmonary bypass: a randomized, controlled trial.

To evaluate the influence of perfusion temperature on systemic effects of cardiopulmonary bypass (CPB), 30 patients undergoing elective coronary artery bypass grafting were randomly assigned to either normothermic (warm, n = 14, 36 degrees C) or hypothermic (cold, n = 16, 28 degrees C) CPB. Serial hemodynamic measurements and blood samples were obtained before, during and after the CPB procedure. During CPB, there were no differences between both groups in the need for vasopressors (norepinephrine, phenylephrine), urinary output, or fluid balance. In the early postoperative period, normothermic CPB patients had significantly lower systemic vascular resistance and higher cardiac index measurements (mean +/- standard error: systemic vascular resistance, 880 +/- 27 versus 1,060 +/- 57 dyne.s.cm-5, p = 0.025; cardiac index, 3.6 +/- 0.1 versus 2.9 +/- 0.1 L.min-1.m-2, p = 0.01) without differences in the administration of vasoactive drugs. Blood loss was significantly higher in patients after hypothermic CPB (median [range] body surface area: 370 [180-560] versus 490 [280-2,120] mL/m2, p = 0.0006), with a greater need for transfusion of erythrocytes and fresh frozen plasma. Plasma levels of tumor necrosis factor and soluble tumor necrosis factor receptors increased during and after CPB, independent of perfusion temperature. This study suggests a significant influence of CPB temperature and respective perfusion management on postoperative hemodynamics and blood loss. Normothermic CPB is not associated with additional systemic adverse effects.

Blood Loss, Surgical↗
PubMed1994

Relation of cyclosporine blood levels to adverse effects on lipoproteins.

Hyperlipidemia is common in renal allograft recipients. To elucidate the role of cyclosporine in posttransplant hyperlipidemia, we measured lipids, lipoprotein lipids, and apolipoproteins of thirty-five renal allograft recipients and evaluated their relation to trough cyclosporine blood levels. All patients were on a triple immunosuppressive regimen with equal doses of prednisone and azathioprine, and had stable graft function. Cyclosporine blood levels were significantly correlated to total plasma cholesterol (P = 0.028), low-density lipoprotein cholesterol (P = 0.022), apolipoprotein B (P = 0.017), and the cholesterol/high-density lipoprotein cholesterol ratio (P < 0.002), but not to plasma triglycerides. Significant inverse correlations were found between cyclosporine blood levels and high-density lipoprotein cholesterol (P = 0.034), high-density lipoprotein3 cholesterol (P = 0.025), and apolipoprotein A-1 (P = 0.047), but not high-density lipoprotein2 cholesterol. The independent relation of cyclosporine blood levels to each of the measured lipid parameters was investigated by a stepwise regression model including age, body mass index, interval from transplantation, diabetes mellitus, plasma creatinine, and intake of diuretics and beta-blockers. After correction for these 7 variables, cyclosporine blood levels remained significantly associated with high-density lipoprotein cholesterol, high-density lipoprotein3 cholesterol, apolipoprotein A-1, apolipoprotein B, low-density lipoprotein cholesterol, and the cholesterol/high-density lipoprotein cholesterol ratio. These data suggest that cyclosporine causes atherogenic dyslipidemia.

Adult↗
PubMed1994

Heparin surface coated hard shell venous reservoirs: experimental evaluation ex vivo.

The present study was designed for ex vivo evaluation of a heparin coated hard shell venous reservoir in comparison to uncoated control reservoirs. An open chest bovine right heart bypass model (n = 9, bodyweight 72 +/- 6 kg) with passive blood drainage from the right atrium into the venous reservoir and active retransfusion into the pulmonary artery (roller pump) was selected for this purpose. Clear priming was used for the open perfusion circuit. No heparin was given before or during the evaluation period which was scheduled for 6 hours. Reservoir blood flow was at the beginning 3.5 +/- 0.6 l/min for coated versus 3.4 +/- 0.3 l/min for uncoated (NS). After 6 hours, blood flow was 3.3 +/- 0.1 l/min for coated versus 2.7 +/- 0.4 l/min for uncoated (p < 0.05). Hematocrit moved from a baseline level of 30 +/- 2% for coated versus 28 +/- 3% for uncoated (NS) to 28 +/- 3% for coated versus 27 +/- 5% for uncoated (NS) after 6 hours. Prebypass platelet levels of 100% in both groups moved to 84 +/- 3% for coated versus 78 +/- 23% for uncoated (NS) after 6 hours. Activated coagulation time (ACT) before bypass was 148 +/- 12 s for coated and 153 +/- 6 s for uncoated (NS). After 6 hours, ACT was 160 +/- 9 s for coated versus 152 +/- 5 s for uncoated (NS). Thrombin time before bypass was 15 +/- 2 s for coated versus 16 +/- 2 s for uncoated (NS).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1993

A new look at the limits of detection (LD), quantification (LQ) and power of definition (PD)

The relationship between the concentration of the analyte and the imprecision of an analytical method can be displayed by the precision profile in which the coefficient of variation (relative standard deviation) is plotted against the concentration of the analyte. The function of the curve of the profile and its confidence limits can easily be assessed by a computer program developed by W.A. Sadler & M.H. Smith (Clin. Chem. 36 (1990), 1346-1350). For the assessment of limits of detection and of quantification the following procedure is proposed: The lower (and upper) limit of the measuring interval is defined by the point at which an acceptable CV-line intersects the confidence limit. If, in the variance function one sets the concentration to zero, the normal distribution of the random errors of the blank will result. The mean of the next adjacent normal distribution, following the variance formula and overlapping the "zero-distribution" by a defined amount, represents the limit of detection. Within the described measuring interval, or within a fraction of it, one might construct overlapping normal distributions in an analogous manner. Their number represents the "power of definition" (PD) (instead of the "analytical sensitivity"), which also depends on the concentration of the determinand according to the variance function. We tested these hypotheses by a comparison of two methods for the determination of cyclosporin A (ciclosporin, INN). Our results demonstrate that the data of the lower limits of the measuring interval and of the limit of detection agree well with data from the literature obtained in extensive interlaboratory surveys.

Algorithms↗
PubMed1993

[Are the advantages of percutaneous catheterization technique gained by increased blood trauma?].

UNLABELLED: The development of thin-walled cannulas which can be introduced by percutaneous technique into femoral artery and vein has revived interest in emergency cardiopulmonary support (CPS). To assess possible blood damaging effect due to the small-bore cannulas, 8 in vitro perfusions were performed with two parallel pump circuits: one circuit with a 17 F arterial and a 21 F venous cannula, one circuit without cannulas (control). The circuits were filled with 61 of bovine blood and run for 6 hours at a temperature of 37 degrees C and a flow of 4 l/min. Serial blood samples were taken before and during the experiment. There was a significantly higher level of free plasma haemoglobin (pl Hb) and a decreased number of leukocytes (Lc) after 6 hours of perfusion in the group with percutaneous cannulas as compared with control (pl Hb: 63.2 +/- 0.3 vs. 26.3 +/- 4.1 mumol/l, p < 0.05, Lc: 2.4 +/- 0.6 vs. 4.6 +/- 0.8 x 10(9)/l, p < 0.05, mean +/- se). CONCLUSION: percutaneous cannulas are associated with an increased blood trauma. If extended cardiopulmonary support is considered (more than several hours), percutaneous cannulas should be replaced by large-bore cannulas.

Animals↗
PubMed1992

Multicentre evaluation of the Boehringer Mannheim/Hitachi 747 analysis system.

Analytical performance and practicability of the new Boehringer Mannheim/Hitachi 747 analysis system were assessed in a multicentre evaluation involving four laboratories. The analytical performance was evaluated according to a protocol similar to the ECCLS guidelines and comprised 13 analytes including enzymes, substrates and electrolytes. About 65,000 results were obtained within three months. The evaluation was planned and supported by a program system called "Computer Aided Evaluation". Acceptance criteria have been established for judging the results. The median of the within-run coefficients of variation (CVs) in control sera of all methods was below 1%, being far below the acceptance limit of 2%. The median of CVs of between-days imprecision was below 2% (acceptance criterion 3%). The high degree of precision prompted us to set up a biometrical model suitable for the differentiation between deviant points, outliers and measurements that can still be explained by the system performance. No relevant drift effects were observed during eight hours. The methods were linear over a wide range, avoiding rerun analysis in most cases. No sample-related carry-over was found. Reagent-dependent carry-over outside the acceptance limits was measured from uric acid to phosphorus to a slight extent, and from triacylglycerols to lipase, as well as from total protein to bilirubin to a perceptible degree. It can be avoided by separating these reagent combinations in the channel arrangement. Taking a systematic deviation of more than 10% as unacceptable, four of the 13 analytes suffered from interference by haemoglobin, one by bilirubin and one by turbidity. The Boehringer Mannheim/Hitachi 747 analysis system is capable of determining serum indices which in combination with the interferogram allow an assessment of the interference. With the exception of chloride the recovery of the assigned values for all control sera showed values between 95 and 105%. Out of 40 method comparison studies for enzymes and substrates, 31 yielded regression equations with less than 5% proportional errors and less than 5% constant errors. Deviations exceeding these acceptance criteria can be explained by differences in the reagent formulation, in the method employed or in calibration. The agreement of the ISE method comparisons was within a +/- 5% deviation over a wide analytical range. Practicability of the Boehringer Mannheim/Hitachi 747 analysis system was assessed with the help of a questionnaire, in which properties of the instrument were quantified, thus permitting a relatively objective rating. The 190 questions were placed in 14 groups, each dealing with an attribute of the instrument.(ABSTRACT TRUNCATED AT 400 WORDS)

Autoanalysis↗
PubMed1992

[Results of multicenter evaluation of reagents for determination of sodium, potassium and chloride ions using enzyme activation].

We present the results of a multicentre evaluation with Boehringer Mannheim/Hitachi instruments of new "enzymatic" methods for the determination of Na+, K+, and Cl- in serum or plasma. The between-day coefficient of variation was less than 1.4% (Na+), less than 2.6% (K+) and less than 1.7% (Cl-). The linear range of the assays were at least 80 to 200 mmol/l (Na+), 1.5 to 17 mmol/l (K+) and about 30 to at least 200 mmol/l (Cl-). The comparisons with routine flame atomic emission spectrometry and coulometry showed a satisfactory agreement of the test results. The "enzymatic" assays are insensitive to even grossly elevated levels of bilirubin and lipids (sodium, potassium, and chloride assays), NH4+ (potassium assay) and amylase (chloride assay). Interference by various drugs was not detected. Since the new methods can easily be adapted to photometric clinical chemistry instruments, they represent a valuable alternative to the use of ion-selective electrodes, flame atomic emission spectrometry and coulometry.

Calibration↗
PubMed1991

[Evaluation of enzymatic methods for the determination of plasma sodium and potassium using the Hitachi 747 analytic system].

In the hospital clinical laboratory, plasma potassium and sodium are usually determined by using a flame photometer or ion-selective electrodes, for which special equipment is required. The new enzymatic methods of sodium and potassium can be used as a routine chemical method. We evaluated the new enzymatic methods for the determination of sodium and potassium in human plasma on the Hitachi 747. The sodium and potassium assay kits were purchased from Boehringer Mannheim, Germany. Precision studies were performed using three levels of pool plasma. The coefficients of variation (CV) of sodium and potassium determination were less than 1.0% and less than 2.2% by intraassay and interassay respectively. The recoveries of the assigned values of the ten control sera were 97-102%. Comparisons with results either from flame photometry and from ion-selective electrodes showed no clinically relevant differences for 100 patient samples. Performance of the enzymatic methods for Na+ and K+ is clinically comparable to flame photometry or ion-selective electrodes in the routine clinical chemistry laboratory.

Clinical Enzyme Tests↗
PubMed1991

Experimental evaluation of heparin-coated cardiopulmonary bypass equipment with low systemic heparinization and high-dose aprotinin.

Cardiopulmonary bypass (n = 8 calves) with heparin-coated perfusion equipment, low-dose systemic heparinization (activated clotting time: ACT greater than 180 s) and high-dose aprotinin administration was evaluated in comparison to standard perfusion equipment with full-dose systemic heparinization (ACT greater than 480). All animals were perfused for 6 hours and similar values were observed for blood gases and mixed venous oxygen saturation in both groups. The heparin doses given in the study group before and during the 6 hours of perfusion totalized 14660 +/- 2553 IU as compared to 60833 +/- 5137 IU for the control group. No protamin was given in the study group whereas an equivalent of 27000 +/- 5805 IU was necessary to reverse heparin in the control group. There was no difference for prebypass hematocrit versus postbypass hematocrit in the two groups. Prebypass plasma hemoglobine was 8.4 +/- 2.1 mumol/L in the study group versus 10.0 +/- 3.8 mumol/L in the control group. After mixing with the priming volume, plasma hemoglobine was 8.6 +/- 2.5 mumol/L in the study group versus 6.7 +/- 1.6 mumol/L in the control group. The highest value was observed in the study group after 2 hours of perfusion (8.2 +/- 2.1 mumol/L) versus 5 hours of perfusion in the control group (7.4 +/- 3.6 mumol/L). Prebypass LDH levels of 1610 +/- 150 IU in the study group versus 1740 +/- 210 IU in the control group moved to 1870 +/- 200 IU in the study group at 24 hours after perfusion versus 2650 +/- 400 IU in the control group at 24 hours and decreased thereafter.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗