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Biomedical subjects

P Perrotta

Publications and source records attributed to P Perrotta.

At least 19 recordsLinked to original sources
PubMed1999

Effect of recombinant human megakaryocyte growth and development factor coupled with polyethylene glycol on the platelet storage lesion.

BACKGROUND: Platelet production is regulated by a thrombopoietic growth factor (Mpl ligand). The receptor for this platelet growth factor (Mpl) is expressed on the platelet surface membrane. A recombinant thrombopoietic cytokine, recombinant human megakaryocyte growth and development factor coupled with polyethylene glycol (PEG-rHuMGDF), was added to apheresis platelets in vitro to determine whether Mpl ligand-receptor binding produced any beneficial or adverse effect on the development of the platelet storage lesion during 5 days of storage. STUDY DESIGN AND METHODS: This study was designed as a dose-response protocol to determine the effects of adding increasing concentrations of PEG-rHuMGDF (0.0 [control], 2.5, 25, and 250 ng/mL) to apheresis platelets stored in two types of plastic storage containers. The increasing concentrations of PEG-rHuMGDF used simulated the theoretical peak plasma level attained in vivo, with an intravenous dose of 0, 0.1, 1.0 and 10 microg per kg of PEG-rHuMGDF. The platelets were stored with agitation at 20 to 24 degrees C for 5 days. A battery of in vitro assays was performed on storage Days 1 and 5, including pH, blood gases, platelet count, lactate dehydrogenase, mean platelet volume, glucose, lactate, osmotic recovery, morphology score, CD62P, and one-dimensional polyacrylamide gel electrophoresis analyses. RESULTS: Analysis of results on both Day 1 and Day 5 showed no significant differences among any of the three PEG-rHuMGDF doses and the control group, for any in vitro assay. One-dimensional polyacrylamide gel electrophoresis showed no changes among the platelet protein patterns for the three PEG-rHuMGDF doses studied or the control. Storage-induced changes, however, did occur equally in all four groups of platelets over the 5 days of storage. CONCLUSION: The addition to stored apheresis platelets of up to 10 microg per kg of PEG-rHuMGDF (250 ng/mL), followed by 5 days of storage at standard conditions, does not appear to promote or retard development of the platelet storage lesion.

Blood Glucose↗
PubMed1988

Perceived social support from family and friends: measurement across disparate samples.

This research investigates the psychometric properties of the Perceived Social Support (PSS; Procidano & Heller, 1983) from family and friends across three samples: a chronic-psychiatric sample, a diabetic sample, and an undergraduate college student sample. Comparisons of internal consistency, central tendency, and dispersion for both the perceived family support (PSS-FA) and the perceived friend support (PSS-FR) are reported, as are the relationships between these two scales for each group. Correlations with general health status and psychiatric symptomatology are also reported. The utility of this measure for generalizable research on social support is discussed.

Adult↗
PubMed1986

Leaving home: later stages of treatment.

While the Leaving Home approach has greatly enhanced the work of many therapists struggling to help troubled young adults and their families, a more thorough consideration of the difficulties that often arise in the later stages of Leaving Home treatment is still needed. This paper examines the ways in which the function of the symptom and difficulties with life-cycle transitions may lead to problems in the later stages of treatment. Common manifestations of later-stage problems, including loss of therapeutic focus and failure of parents to follow through on plans are examined. Direct and indirect strategies for addressing these problems are identified and guidelines for their use presented.

Acting Out↗
PubMed1981

Medical student attitudes toward geriatric medicine and patients.

This study examines the influence of factual knowledge of the aged, general attitudes toward the aged, and personal contact with the aged on first-year medical students' attitudes toward geriatric patients and geriatric medicine. Entering medical students indicated a preference for working with younger patients rather than aged patients. Students' attitudes toward the aged were associated positively with their knowledge of the aged, but their interest in geriatric medicine did not appear to be affected significantly by knowledge of, attitudes toward, or personal contact with the aged. The results suggest that factors beyond those considered in this study may need to be examined if there is to be an increase in the number of physicians wishing to care for the elderly.

Aged↗