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P Pouliot

Publications and source records attributed to P Pouliot.

12 recordsLinked to original sources
PubMed1989

A potential polyvalent Neisseria meningitidis vaccine based on a mixture of CaCl2 cell extracts from groups A, B and Y.

Outer membrane proteins (OMP) from Neisseria meningitidis cells of groups A, B, C and Y were extracted with CaCl2. Sodium dodecyl sulphate polyacrylamide gel electrophoresis of each extract showed a multibanded pattern characterized by the presence of three to four major proteins. Immunization of mice with individual extracts induced homologous bactericidal reactions. In addition, the extracts from groups B and C induced heterologous bactericidal reactions (B-C, C-B). The sera of mice immunized with a mixture of group A, B, and Y extracts showed a high bactericidal titre against the three N. meningitidis groups forming the mixture. Moreover, this bactericidal activity had a large spectrum against different strains from groups A, B, Y and also C. After immunization of mice with the individual extracts, each of the sera was shown by immunoblotting to contain antibodies reacting with some of the corresponding proteins. Mice immunized with the mixture showed a significant reduction of bacteraemia following challenge with either of N. meningitidis groups A, B or C. These results suggest that a mixture of OMP from different N. meningitidis groups may have the potential for a meningococcal polyvalent vaccine.

Animals↗
PubMed1977

T lymphocytes and the transfer of immunity to Trypanosoma musculi in mice.

Trypanosoma musculi produces a resolving infection in mice and the immune response is thymus dependent. Spleen cells from immune and from uninfected mice were transferred to T cell-deprived mice and restored their ability to control the infection, the immune cells being effective most rapidly. Treatment of the cells in vitro with anti-theta serum did not impair their ability to restore immunocompetence and it is proposed that, though T-cell dependent, the immune response is effected by theta- cells.

Animals↗