[The Regulation of Aggressive Animals and the Staffordshire terrier].
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Aggression↗
Biomedical subjects
P Prins
Publications and source records attributed to P Prins.
GenEST, a powerful bidirectional link between cDNA sequence data and gene expression profiles generated by cDNA-AFLP.
The release of vast quantities of DNA sequence data by large-scale genome and expressed sequence tag (EST) projects underlines the necessity for the development of efficient and inexpensive ways to link sequence databases with temporal and spatial expression profiles. Here we demonstrate the power of linking cDNA sequence data (including EST sequences) with transcript profiles revealed by cDNA-AFLP, a highly reproducible differential display method based on restriction enzyme digests and selective amplification under high stringency conditions. We have developed a computer program (GenEST) that predicts the sizes of virtual transcript-derived fragments (TDFs) of in silico-digested cDNA sequences retrieved from databases. The vast majority of the resulting virtual TDFs could be traced back among the thousands of TDFs displayed on cDNA-AFLP gels. Sequencing of the corresponding bands excised from cDNA-AFLP gels revealed no inconsistencies. As a consequence, cDNA sequence databases can be screened very efficiently to identify genes with relevant expression profiles. The other way round, it is possible to switch from cDNA-AFLP gels to sequences in the databases. Using the restriction enzyme recognition sites, the primer extensions and the estimated TDF size as identifiers, the DNA sequence(s) corresponding to a TDF with an interesting expression pattern can be identified. In this paper we show examples in both directions by analyzing the plant parasitic nematode Globodera rostochiensis. Various novel pathogenicity factors were identified by combining ESTs from the infective stage juveniles with expression profiles of approximately 4000 genes in five developmental stages produced by cDNA-AFLP.
Animals↗
Comparing hospital perinatal mortality rates: a quality improvement instrument.
This article describes the development of a reliable measurement instrument for the quality of perinatal care. The Hospital Perinatal Mortality Comparison (HPMC) predicts the expected perinatal mortality for individual hospitals, based on the perinatal mortality experienced in a group of similar newborns in a large reference population. Out of the 76 hospitals analyzed in 1990, 2 performed significantly better and 5 performed significantly worse than expected according to the logistic regression model. These results may lead to the identification of opportunities for improving the process of medical care in these hospitals.
Belgium↗
General method for analysing dentist-patient interaction.
The interaction between dentist and patient during dental treatment may influence the frequency of dental visits in the future because this interaction may increase the dental fear of the patient. To find the characteristics of the dentist's behavior that increase or decrease dental fear a statistical analysis of the interaction may be used. Data of such interactions are known as time series. Because it is highly probable that the behavior of an individual at two different points in time is autocorrelated a correct statistical analysis of the interaction between dentist and patient must take this possibility into account. A general statistical method is proposed that can take autocorrelations into account. The method is compared with a simpler method that may not deal adequately with autocorrelations.
Child↗
Interactions between dentists and anxious child patients: a behavioral analysis.
The aim of this investigation was to study the effect of dentists' behavior on the (anxious) behavior of child patients. Twelve high-anxious and a matched group of 12 low-anxious children from 6 to 12 yr of age were selected and randomly assigned to one of six dentists: three with experience in treating fearful children and three without such experience. Each child was treated twice. The first treatment was prophylactic, the second, 2 wks later, consisted of preparation and restoration of a cavity (Class 1) under local anesthesia. While both treatment sessions were videotaped, only the latter one was used in this study. Data analysis which did not control for autocorrelations revealed 22 (out of 28) significant effects. Data analysis with a control for autocorrelations showed only two significant effects: 'working contact', which decreased fear-related behavior and 'no physical contact', which increased fear-related behavior.
Anxiety↗
Behavior of dentists and child patients during treatment.
This study is part of a larger research project which aims to analyze children's dental fear by making a detailed analysis of the behavioral interactions between dentists and anxious child patients. The behavior of 12 high- and 12 low-anxious children was recorded on videotape during two dental visits in which the children were treated by either experienced or inexperienced dentists. Results indicated a relation between the dentist's experience in treating anxious children and the behavior of child patients. In general, children treated by experienced dentists showed more fear-related behaviors. Furthermore, the behavior of the dentist was related to both his experience and the fear level of the child patient. Experienced dentists showed more communicative behaviors than the inexperienced dentists, but all dentists communicated more with high- than with low-anxious children. Experienced dentists worked faster than inexperienced dentists, but treatment of the high-anxious children was not more time-consuming than treatment of the low-anxious children.
Anxiety↗