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Biomedical subjects

P R Orange

Publications and source records attributed to P R Orange.

4 recordsLinked to original sources
PubMed2004

Functional genomics and proteomics: application in neurosciences.

The sequencing of the complete genome for many organisms, including man, has opened the door to the systematic understanding of how complex structures such as the brain integrate and function, not only in health but also in disease. This blueprint, however, means that the piecemeal analysis regimes of the past are being rapidly superseded by new methods that analyse not just tens of genes or proteins at any one time, but thousands, if not the entire repertoire of a cell population or tissue under investigation. Using the most appropriate method of analysis to maximise the available data therefore becomes vital if a complete picture is to be obtained of how a system or individual cell is affected by a treatment or disease. This review examines what methods are currently available for the large scale analysis of gene and protein expression, and what are their limitations.

Brain↗
PubMed1996

Allelic variations of the human histamine H2 receptor gene.

The gene encoding the human histamine H2 receptor (H2R) has previously been cloned and sequenced from gastric cDNA. Following PCR amplification of a fragment of the H2R gene from total human DNA, three single nucleotide base substitutions were observed and confirmed when compared with the previously published sequence. One of these base changes introduces an additional TaqI restriction endonuclease site in the coding portion of the gene. PCR amplification of human H2R gene fragments followed by cleavage with TaqI demonstrated the existence of allelic variation of the human H2R gene.

Alleles↗
PubMed1996

Individuals with schizophrenia have an increased incidence of the H2R649G allele for the histamine H2 receptor gene.

We have previously described an allelic variant of the human H2R, nominated the H2R649G allele. This allele contains an adenine-->guanidine substitution at base 649, which introduces an additional TaqI restriction endonuclease site into the gene. With this in mind, we have investigated allelic polymorphism of this receptor and its association with schizophrenia. H2R DNA from 47 schizophrenic patients and 46 control subjects was amplified by the polymerase chain reaction (PCR). These PCR products were analyzed by observing TaqI cleavage patterns and single-stranded conformational polymorphisms. It was found that the H2R649G allele was 1.8 times more frequent in the schizophrenic population (chi 2 test P < 0.01). In addition, schizophrenic individuals were 2.8 times more likely to be homozygous for the H2R649G allele than the control population, (chi 2 test P < 0.05). These data place the attributable fraction for possession of the H2R649G allele at 28.4%.

Alleles↗