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P Radice

Publications and source records attributed to P Radice.

70 records · Page 4Linked to original sources
PubMed1988

Monoclonal antibodies against NIH 3T3 cells transformed by human thyroid carcinoma DNA.

First cycle transformants of NIH 3T3 cells transfected with metastatic human thyroid carcinoma DNA were used as immunogen to obtain monoclonal antibodies (MAbs) against normal and transformation-related antigens. The transformed cell line (M33) was shown to contain Alu sequences. Two MAbs were selected on the basis of their differential reactivity toward untreated NIH 3T3 cells or the transformed M33 cell line. By immunofluorescence, immunoelectronmicroscopy and biochemical analysis, the first MAb (MTr1) was demonstrated to recognize an epitope on cytoskeletal filaments of proliferating murine fibroblasts. Similar MTr1-labelled filaments were also found to accumulate into cytoplast-like structures spontaneously produced by M33 cells. The characterization by immunofluorescence of MTr2, the second MAb, indicates that it recognizes a specific human antigen associated with normal thyroid epithelial cells and differentiated thyroid tumors.

Antibodies, Monoclonal↗
PubMed1987

HRAS1 proto-oncogene polymorphisms in human malignant melanoma: TaqI defined alleles significantly associated with the disease.

We have analysed the DNA of peripheral blood leukocytes (PBL) from 55 melanoma patients and 53 healthy individuals and failed to find any significant association between melanoma and rare HRAS1 alleles defined by MspI/HpaII digestion. However, the analysis of the same DNAs for a different polymorphism based on the presence of additional TaqI sites in the variable tandem repeat region of HRAS1 showed that the total frequency of a group of allelic variants, named Tp, was significantly higher in melanoma patients than in normal donors.

DNA, Neoplasm↗
PubMed1986

Detection of two TaqI polymorphisms in the VTR region of the human HRAS1 oncogene.

Restriction enzyme analysis of the variable tandem repetition (VTR) region of the human HRAS1 oncogene revealed two TaqI polymorphisms. The first polymorphism overlaps that detected with MspI/HpaII restriction enzymes and is due to a variable number of repeat units which form the VTR region at the 3' end of the oncogene. The second polymorphism appears to be due to two new TaqI restriction sites created within the VTR region itself. This novel polymorphism was detected in 26 of 145 human DNA samples and was found to segregate in a Mendelian fashion.

Chromosome Mapping↗
PubMed1985

Cell surface antigens of chemically induced fibrosarcomas: detection by a monoclonal antibody of a tumor-restricted Mr 12,000 protein gag antigen encoded by a dual-tropic murine leukemia virus.

Fusion products of spleen cells of W/FuDp rats immunized with a methylcholanthrene-induced BALB/c sarcoma, CA-2, and mouse myeloma cells were screened in an attempt to identify a monoclonal antibody defining the individually distinct tumor-specific transplantation antigen of CA-2. A hybridoma, MP/69/04, was isolated which produces an IgG2a monoclonal antibody that recognized a tumor-restricted antigen of CA-2. In direct binding assay, MP/69/04 reacted only with 2 of 15 methylcholanthrene induced BALB/c sarcomas tested. Thymus, spleen, lymph nodes, bone marrow, brain, adult lung fibroblasts, newborn muscle fibroblasts and 3T3 cells were negative. Absorption tests revealed, however, expression of the MP/69/04 determinant on 8 of the 12 murine leukemia virus (MuLV) producer BALB/c sarcoma tested. The antigen was not detected on any of the three non-producer sarcomas tested nor on a wide range of normal tissues and cell lines. An N-dualtropic MuLV was isolated from CA-2, and cell lines susceptible to infection by this virus were shown to express the MP/69/04 epitope. By Western blotting, the MP/69/04 epitope was identified as being expressed on the MuLV structural protein with a molecular weight of 12,000, present in CA-2 cells and in the purified CA-2 MuLV. These results indicate the MP/69/04 antigen is not a unique tumor-specific transplantation antigen but is a gag product of a recombinant retrovirus which is expressed on the cell surface of many MuLV + methylcholanthrene-induced BALB/c fibrosarcomas.

Animals↗
PubMed1984

Integration and expression of MCF-13 provirus in MCF-13-induced lymphomas.

Polytropic viruses were isolated from 11 MCF-13-induced lymphomas using multiple infectious cycles on the mink lung and SC1 cell lines. The viruses were characterized by host range infectious properties and by biochemical properties using Cleveland's polypeptide mapping of the p30 and gp 70 viral proteins demonstrating identity in their biological properties with the MCF-13 lymphoma-inducing virus. Restriction enzyme analysis of the high molecular weight tumoral DNAs confirmed the presence of proviral sequences homologous to the inducing provirus in the lymphoma genomes indicating that MCF-13 MuLV has integrated in the cellular genome of the induced lymphomas.

Animals↗
PubMedDate not supplied

Loss of polymorphic restriction fragments of class I and class II MHC genes in a malignant melanoma.

DNAs from human malignant melanoma cells and autologous peripheral blood lymphocytes were evaluated by Southern blot analysis with probes for class I and II HLA genes. DNA of melanoma cells digested with PvuII, EcoRI and BglI and hybridized with a DR beta probe showed a loss of several fragments when compared with DNA from lymphocytes. The same DNAs were not distinguishable when hybridized with a DQ beta probe. Analysis of melanoma and autologous lymphocyte DNAs from the same patient with a class I cDNA, after digestion with several endonucleases, revealed a further loss of fragments in melanoma cells. Comparison of restriction fragment patterns of melanoma and lymphocytes with those of homozygous, serologically-typed cell lines indicated that melanoma cells have lost fragments diagnostic of DR2 and A1 antigens. A densitometric analysis of signals of several oncogenes in comparison with that of DR indicated that a duplication of the remaining DR allele had occurred in melanoma cells.

DNA, Neoplasm↗
PubMedDate not supplied

Molecular genetics of hereditary non-polyposis colorectal cancer (HNPCC).

The story of the molecular genetics of HNPCC is one of astonishingly rapid achievements. In just 16 months, from May 1993 to September 1994, four different genes, namely hMSH2, hMLH1, hPMS1 and hPMS2 have been identified and demonstrated to be associated with the disease. Their cloning was facilitated by the finding that tumor cells in HNPCC patients display a hypermutability of DNA short tandem repeats (microsatellite instability). In fact, HNPCC associated genes are the human counterparts of genetic elements known to control the fidelity of DNA replication in lower organisms. So far, more than 50 germline mutations of hMSH2 and hMLH1 genes have been reported in HNPCC kindreds. In addition, somatic mutations have been documented in hereditary as well as sporadic cancers. Unfortunately, the molecular diagnosis of HNPCC is hampered by the lack of mutational "hot spots" and of clearly defined genotype-phenotype correlations and different screening methods are to be employed for the analysis of affected and at-risk individuals.

Colorectal Neoplasms, Hereditary Nonpolyposis↗
PubMedDate not supplied

Algorithm to correct hyperopic astigmatism with the Nidek EC-5000 excimer laser.

BACKGROUND: The efficacy of a new ablation algorithm for the correction of hyperopic astigmatism with the Nidek EC-5000 excimer laser was evaluated. METHODS: Twenty-five eyes with mean preoperative hyperopia of +3.76 +/- 1.70 D and a mean hyperopic cylinder of 2.20 +/- 0.80 D underwent photorefractive keratectomy (PRK) using a new algorithm with the Nidek EC-5000 excimer laser (software version 3.0). The new algorithm differed from previous algorithms in that less tissue was removed for the same amount of diopters, and there was less of a dioptric gradient between the optical zone and the transition zone. Mean preoperative spectacle-corrected visual acuity was 0.8 +/- 0.09. Minimum follow-up was 6 months. RESULTS: Mean postoperative spectacle corrected visual acuity (geometric mean) increased significantly to 0.89 +/- 0.1. The mean sphere decreased by 3.08 D and the mean cylinder by 1.60 D. CONCLUSION: Hyperopic PRK using the Nidek EC-5000 excimer laser with this new algorithm for hyperopic astigmatism appears to be safe and effective.

Adult↗