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P Rajendran

Publications and source records attributed to P Rajendran.

31 records · Page 2Linked to original sources
PubMed2000

Identification of Pneumocystis carinii in induced sputum of AIDS patients in Chennai (Madras).

Induced sputum samples were collected from 32 AIDS patients with respiratory ailments. Pneumcystis carinii was demonstrated in 9 out of 32 AIDS cases by Indirect Immunofluorescence technique (HF). Four cases were positive by all the three techniques namely Giemsa staining, Toluidine blue staining and IIF, three were positive by both toluidine blue and IIF, and two were positive only by IIF. Among other microbial pathogens, acid fast bacilli was demonstrated in all the P carinii positive cases and Candida albicans in 53% AIDS cases from the induced sputum sample.

AIDS-Related Opportunistic Infections↗
PubMed1999

Clinical, bacteriologic and histopathologic studies on induced leptospirosis in stray dog pups.

Eighteen 8-12 days old stray dog (canis familiaris mongrel) pups of either sex; 6 pups each in test groups and control group were infected with lepotspiral serovars autumnalis and canicola. The experimental animals, clinical, bacteriologic and histopathologic kinetics were observed. Both the serovars had evoked typical clinical manifestations. Leptospiraemia could be demonstratedin between the post inoculation (PI) days 1 & 5. Leptospiruria commensed in between the PI days 5 & 7 and lasted throughout the study period. Histopathologic study did not reveal any marked pathologic changes except hydropic changes in the liver of both the test groups.

Animals↗
PubMed1993

Antiviral properties of the seed extract of an Indian medicinal plant, Pongamia pinnata, Linn., against herpes simplex viruses: in-vitro studies on Vero cells.

Pongamia pinnata, Linn., an Indian medicinal plant used in the Ayurvedha and Siddha traditional medicine systems, for treatment of clinical lesions of skin and genitalia, was evaluated for antiviral properties against herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) by in-vitro studies in Vero cells. A crude aqueous seed extract of P. pinnata completely inhibited the growth of HSV-1 and HSV-2 at concentrations of 1 and 20 mg/ml (w/v), respectively, as shown by complete absence of cytopathic effect.

Animals↗
PubMed1989

Observations on human bone-marrow for Leishmania donovani.

During the recent epidemic in North Bihar, a total of 65 cases which were clinically designated as Kala-azar had been subjected to bone-marrow aspiration from the iliac crest, out of which the L.D. body was demonstrated in 55 cases (84.61 per cent). Culture analysis of bone-marrow on modified Tobie's medium, revealed that ten cases (15.39 per cent) which were negative for L.D. body did not show any leptomonads, whereas out of 55 L.D. body positive cases, the promastigote forms developed in 22 cases (40.00 per cent). The probable reasons for the negative findings and also the age and sex distribution in Kala-azar are discussed in brief.

Adolescent↗
PubMedDate not supplied

Herpes simplex keratitis in South India: clinico-virological correlation.

PURPOSE: A retrospective cross-section study to analyze the prevalence of herpes simplex virus-induced keratitis (HSK) among 3,000 patients attending a corneal clinic in South India between 1995 and 1997, and to evaluate laboratory techniques for detecting HSK. METHODS: The clinico-virological correlation was studied using herpes simplex virus (HSV) isolation on the Vero cell line, HSV-specific antigen detection by indirect immunofluorescence (IF) microscopy, and serum anti-HSV IgG quantitation, IgM estimation, and tear secretory IgA (sIgA) detection by ELISA. OBSERVATIONS: HSK had a prevalence of 7.8% (234 patients) in this study. A virological correlation could be obtained in 44.4% of the cases that had epithelial manifestations and in 14.8% of the cases that had only stromal disease. In 161 cases where both culture and IF microscopy were used, IF detected 27 cases (26.8%) more than cell culture. The difference in sensitivity between cell culture and IF was found to be statistically significant (McNemar's test, P < .05). An elevation in IgG titer was seen in 17 (30.4%) cases. IgM was detected in only 2 cases of the 62 (3.2%) analyzed. Of the 138 cases analyzed, sIgA was positive in 28 (20.3%) cases. A proved diagnosis could be made in 58% of cases when the specimen was collected during the first week after disease onset, and in only 5% when the time interval increased to 4 weeks. CONCLUSIONS: HSV antigen detection by indirect IF is a rapid and sensitive diagnostic tool for HSK. Tear secretory IgA (sIgA) is a specific marker for acute herpetic keratitis, and the detection of HSV-specific tear sIgA is a valuable adjunct to virus isolation and antigen detection in the laboratory diagnosis of HSK. For a successful diagnosis, the specimen should be collected as soon as possible after HSK onset.

Adolescent↗
PubMedDate not supplied

Toxicity assessment of nickel using Aspergillus niger and its removal from an industrial effluent.

Chemical analysis of electroplating effluent revealed the presence of very high concentrations of nickel (393 ppm) in the effluent. Bioassay was carried out to test the toxicity of nickel chloride to Aspergillus niger. In contrast to 50% conidial inhibition at 1.7 mM nickel, hyphal extension was affected even at a lower concentration (0.4 mM), suggesting that hyphae are more sensitive than conidia to nickel. An increase in nickel concentration resulted in a proportionate decrease in the hyphal extension. Nickel (II)-resistant mutants of A. niger M1, M2, and M3, were obtained using direct selection, stepwise adaptation, and ultraviolet mutation techniques. Biosorption of Ni (II) by the mutant M3 was 50% more than that of its parent strain.

Aspergillus niger↗
PubMedDate not supplied

Community prevalence of sexually transmitted diseases and human immunodeficiency virus infection in Tamil Nadu, India: a probability proportional to size cluster survey.

BACKGROUND: Human immunodeficiency virus (HIV) infection and AIDS is threatening the survival of many nations. To evaluate ongoing interventional strategies and burden of illness estimates, valid data on the prevalence of HIV are required. Often, in the absence of community prevalence data, estimates are based on surrogate markers such as prevalence of HIV in antenatal clinics. Even though the antenatal prevalence of HIV is easier to measure and can be repeated for evaluation, it is important to establish the association between antenatal and community prevalence of sexually transmitted diseases (STDs) and HIV, so that the validity of the estimates can be verified. METHODS: A 'probability proportional to size' cluster survey was conducted in three randomly selected districts of Tamil Nadu in India. The basic unit of the survey was households from rural and urban clusters. Adults 15-45 years of age from the selected households were eligible for recruitment. Demographic, behavioural and laboratory data were collected. Clinical examination was done to identify STD syndromes and blood, urine, vaginal/urethral and endocervical swabs were taken for laboratory diagnosis of STDs from the subjects. Direct smear examination for Trichomonas vaginalis; serological tests for syphilis, hepatitis B, HIV, herpes simplex virus 2, Chlamydia trachomatis; and culture of Neisseria gonorrhoeae and Haemophilus ducreyi were performed on the collected specimens. The data were analysed adjusting for cluster effect. RESULT: We selected and screened 1981 individuals (1157 women and 824 men) for STDs and HIV from 1114 households representing the 25 million projected adult population of Tamil Nadu. The overall community prevalence of STDs including HIV and hepatitis B in Tamil Nadu was 14.6% (CI: 14.1-15.1), and 8.3% (CI: 7.9-8.6) when HIV and hepatitis B were excluded. Community prevalence of HIV and hepatitis B infection was 1.8% (CI:1.7-1.9) and 5.3% (CI: 5.1-5.5), respectively. The distribution of HIV involved both rural and urban regions of Tamil Nadu. On clinical examination, at least one STD syndrome was noted in 486 (24.5%) of the women subjects; vaginal discharge was the most common and found in 421 women (38.4%). CONCLUSION: The prevalence of STD and HIV in Tamil Nadu is higher than expected and has extended into the non-high risk population (generalized epidemic).

Adolescent↗