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Biomedical subjects

P Riedl

Publications and source records attributed to P Riedl.

At least 19 recordsLinked to original sources
PubMed2001

PsaA (pneumococcal surface adhesin A) and PspA (pneumococcal surface protein A) DNA vaccines induce humoral and cellular immune responses against Streptococcus pneumoniae.

Streptococcus pneumoniae is one of the most important human pathogens and improvement of the currently used polysaccharide vaccines is being pursued. We constructed DNA vaccine vectors containing either the full-length psaA (pneumococcal surface adhesin A) or a truncated pspA (pneumococcal surface protein A--pspA') gene. Both constructs showed transient expression of the antigens in vertebrate cells and induced significant antibody response to the pneumococcal antigens in BALB/c mice injected intramuscularly (i.m.). Fusion with an N-terminal cytoplasmatic SV40 T-antigen (CT-Ag), which was previously shown to stabilize poorly expressed antigens through association with Hsp73, also induced anti-PspA antibody response. The induction of antibodies with a low IgG1:IgG2a ratio and elevated gamma interferon (IFN-gamma) production by spleen cells elicited by DNA vaccination indicate preferential priming of Th1 immunity. Since induction of antibodies against both PsaA and PspA was previously shown to correlate with protection against fatal infection with S. pneumoniae and cell-mediated immune responses could contribute to protection, further evaluation of PsaA and PspA as antigens for a DNA vaccine against S. pneumoniae could be promising.

Adhesins, Bacterial↗
PubMed2001

Priming of immune responses to hepatitis B surface antigen with minimal DNA expression constructs modified with a nuclear localization signal peptide.

Nuclear localization signal (NLS) peptides conjugated to DNA increase transfection efficiency in vitro. We tested in mice whether conjugation of NLS peptides to DNA vaccines enhances their immunogenicity after intramuscular injection or gene gun mediated intradermal delivery. We constructed the plasmid pMOK-HBsAY that contains a transcription unit encoding hepatitis B surface antigen (HBsAg) and bacterial sequences for amplification of plasmid DNA. From this plasmid we derived the minimal expression construct pMOK-HBsAY-MIDGE, a covalently closed linear DNA that contains only the HBsAg transcription unit. Both constructs stimulated similar (predominantly IgG1) antibody response to HBsAg after gene gun immunization. In contrast, pMOK-HBsAY plasmid DNA was more efficient than pMOK-HBsAY-MIDGE DNA in priming predominantly IgG2a antibody responses to HBsAg after intramuscular injection. Both constructs efficiently primed cytotoxic T lymphocyte responses after intramuscular immunization. When a NLS peptide was coupled to the pMOK-HBsAY-MIDGE DNA, HBsAg transfection efficiency in vitro and priming of antibody responses to HBsAg after intramuscular (but not gene gun mediated) injection was enhanced 10- to 15-fold. These data show: (a) MIDGE constructs can be used as DNA vaccines indicating that bacterial sequences are not essential cofactors; and (b) in intramuscular (but not gene gun mediated) delivery the immunogenicity of a MIDGE-based vaccine is enhanced by coupling NLS peptides to the vector DNA.

Animals↗
PubMed1988

In vivo effects of recombinant human interleukin 2 on antitumor and antiviral natural immunity in induced or natural murine immunodeficiency states.

We have examined the ability of in vivo treatment of mice with recombinant interleukin 2 (rIL-2) to affect natural immunity measured against tumor (YAC-1) or virally infected (herpes simplex type 1) target cells. rIL-2 treatment leads to significant increases in natural killer/lymphocyte-activated killer (NK/LAK) function and spleen cells recovered. This effect is dose dependent and strain related. The latter parameter correlated with the pretreatment NK activity level of the strain. The rIL-2 induced NK/LAK augmentation is also kinetically restricted as treatment must have occurred within 48-72 h of assay to be effective. The rIL-2 therapy effectively enhances both antitumor and antiviral NK/LAK activity and results in a noticeable increase in asialo-GM1-positive cells in the spleens of treated mice as well as a significant increase in IL-2 receptor expression as monitored by either cytometry or radioligand binding. In vivo treatment of mice with an antibody directed to the ASGM1 determinant effectively reduces the rIL-2 augmentation of both antitumor and antiviral activity even though this treatment does not affect the pretreatment level of antiviral activity. Various natural and induced immunodeficiency states (immunotherapy, irradiation, immunosuppressive drugs, cytoreductive drugs) have been examined for the ability of in vivo treatment with rIL-2 to enhance NK/LAK activity. In vivo rIL-2 administration is differentially effective in enhancing NK/LAK activity in these situations. Notably, in these induced immunodeficiency states, although NK/LAK activity is commonly enhanced, the number of spleen cells recovered often is only marginally affected. Thus, as expected, a limiting aspect in this use of a natural immunomodulator is the number of potentially responsive cells present in the immunodeficiency condition. In addition, correlations between rIL-2 effect, several of the immunodeficiency states, and vascular leak syndrome are briefly discussed.

Animals↗
PubMed1985

Long-term results after sclerotherapy of the spermatic vein in patients with varicocele.

Ninety-seven patients received transfemoral sclerotherapy of the spermatic vein for varicocele. After observation for 2 to 5 years, follow-up was available in 69 patients (71.2%), in 11 of whom the procedure failed (16.0%). Complications during angiography (N = 12, 12.4%) or sclerotherapy (N = 31, 32.0%) did not require hospital treatment. At least one parameter of the spermiogram was improved in 25 of 32 patients (78.1%). Forty-four of 69 patients (63.8%) treated because of a childless marriage, and whose wives were not known to be infertile, had a pregnancy rate of 47.7%. Thi method may be considered equal to surgical procedures in achieving venous occlusion; furthermore it is simple and can be used on an outpatient basis without anesthesia.

Adult↗
PubMed1981

A new method of transfemoral testicular vein obliteration for varicocele using a balloon catheter.

Selective obliteration of the testicular vein as a treatment for varicocele is described in 51 patients. Twenty-five patients had a history of infertility. A coaxial catheter-balloon catheter system was used. The procedure was done on an outpatient basis. The complications and success rates are comparable to postoperative results. Four of the 25 patients whose primary indication for the procedure was infertility have fathered children.

Adolescent↗
PubMed1981

[Methods of diagnosis and treatment of idiopathic varicocele in Germany, Austria and Switzerland (author's transl)].

Replies from 115 urological departments, which were part of a general inquiry within Germany, Austria and Switzerland, were evaluated. 37% of all departments perform a varicocelectomy already after the 6th year of age, 68% after 10 years of age. A preoperative phlebography is performed regularly only by 8%, but the phlebography prior to recurrent surgery is carried out by 72%. The most frequent operative method is the Bernardi technique in 39%, the method of Palomo, ligation superior to the internal inguinal ring and lumbal ligation of the spermatic vein are used equally often. The additional dissection of accompanying veins at the level of internal inguinal ring was reported only by 24%. A postoperative inspection with palpation control is regularly performed by 85%, with semen analysis in 58%. No department reported, postoperative results with sperm analysis for more than one year.

Adolescent↗
PubMed1980

[Partial cirrhosis of the kidney after radiotherapy of a colon carcinoma. Case record (author's transl)].

The radiological symptoms of excretion urography and angiography of a partial radiogenic cirrhosis of the kidney is discussed on the basis of a case of operated colon carcinoma with subsequent curative radiotherapy because of malignant infiltrations into the adjacent adipose tissue. Furthermore, a relationship between radiological morphology and pathophysiology of the chronic radionephritis is established.

Angiography↗
PubMed1980

[Catheter embolization of inoperable kidney neoplasms with the GAW-spiral].

On the basis of 4 cases with inoperable hypernephroma the possibilities of the transfemoral catheter embolisation with the GAW-spiral are discussed. Simplicity of the technique and advantages in comparison to the material embolisation methods are discussed. The case in question was in 2 patients a primary embolisation, in 2 patients a secondary one with recanalised material embolisation. Up to now the patients were controlled over 3 months after the vascular occlusion. A recanalisation could not be observed, rather by total occlusion a tendency to the formation of collateral circulations.

Adenocarcinoma↗
PubMed1979

[Follow-up angiography after palliative renal artery occlusion with the GAW-(Gianturco-Anderson-Wallace)-spiral (author's transl)].

Follow-up angiograms were carried out in six patients after palliative renal artery embolisation for hypernephromas using a GAW-spiral. The following conclusions were reached: it is possible to obtain complete occlusion of the renal artery with cessation of haematuria, reduction in tumour size and in the extent of the pathological vessels. It is necessary, however, to place the spiral accurately in the vessel to be occluded and to embolise all major arteries supplying the tumour. Even when the renal artery has been completely occluded, tumour growth results in the development of varying extent. In order to reduce this collateral circulation, vessels to the tumour periphery should be embolised with a suitable substance before spiral occlusion is carried out.

Adenocarcinoma↗
PubMed1978

[Osteoid osteoma. (Plain films, angiography and scintigraphy in fifty patients) (author's transl)].

The radiological diagnosis and differential diagnosis of osteoid osteomas was considered in relation to a series of 50 histologically verified cases. In twenty, angiography had been performed and scintigraphy was carried out seven times. Various problems in the diagnosis of osteoid osteomas in relation to their localisation within bone (cortical, medullary, sub-periosteal, subarticular) and within the skeleton (near the hip, in the hand or foot, in the spine and in flat bones) are discussed.

Adolescent↗
PubMed1978

[The problem of angiographic diagnosis of giant cell tumors (author's transl)].

The value of angiography in the diagnosis of giant cell tumors of the bone was investigated in 12 patients listed in the Viennese bone tumor register. It could be shown that angiographic criteria of malignancy can frequently be found in grade II cell tumors. Therefore angiography does not seem to be helpful in differentiation and prediction of benign and malignant course of giant cell tumors.

Adolescent↗
PubMed1976

[Late results of the Noble operation. Clinical and radiological follow-up examination].

The results of 58 Noble operations performed during the period between 1964 and 1973 are reported. A partial operation was carried out in 9 cases. In 4 cases there were fatal complications due to technical errors such as too long a terminal ilial loop and direct suture to the intestine. Two cases of late ileus were observed after 2 and 6 months respectively following a partial plication. Follow-up examination at an average of 4 years after Noble operation showed that 26 patients were free of complaint. The Noble operation is best suited for cases with chronic recurrent adhesion ileus when a permanent folding of the small intestine is necessary. For other indications, the newer alternative procedures. (Childs and Phillips and Reiffersceid and Phillip's small intestine splinting) should be used.

Humans↗