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Biomedical subjects

P Rommel

Publications and source records attributed to P Rommel.

At least 19 recordsLinked to original sources
PubMed1990

[Comparative studies of superovulation in high-producing cows].

PMSG-PG (1), PMSG-PG-anti-PMSG (2), and FSH-PG (3) were used under production conditions for superovulation treatment of high-yielding cows. An analysis of results has shown the FSH variant (3) to be superior with significance to the other two regimes. It yielded positive ovarian reactions (greater than or equal to 3 corpora lutea) in 92.0 percent of all cases, with 7.0 +/- 6.3 transferable embryos being obtained. Regime 1 gave only 75.5 percent as well as 3.2 +/- 2.97, while 85.5 percent and 4.3 +/- 3.9 were recorded from regime 2. Significance amounted to P less than 0.01; 0.001. Regimes 2 and 3 in standardised form are recommended for embryo transfer with cattle in breeding practice.

Animals↗
PubMed1990

[The hormone profile in the blood plasma of heifers after cycle synchronization with prostaglandin F2alpha].

Highly sensitive radio-immuno assay (RIA) was used for 21 days to study the curves of several steroid hormones, progesterone (P), oestradiol 17 beta (E2), and testosterone (T), as well as of luteinizing hormone (LH) in peripheral blood of six heifers to which oestrus had been induced by prostaglandin F2 alpha. The validity of the authors' RIA for P, E2, and T determination in blood plasma was positively verified by two reliability criteria, correctness and accuracy, as well as by comparative determinations, using reference methods. Only four of the six heifers returned to oestrus, within 21 days from first oestrus induction. A typical cycle-related curve of the P concentration in peripheral blood with peak values between the 13th and 17th days of cycle (15-26 nmol/l) was recorded from four animals. The peak values of pre-ovulatory E2 and LH were between 33.0 and 53.2 pmol/l or between 19.5 and 52.5 micrograms/l. Some of the T rises in peripheral blood were in parallel to E2 concentrations. All hormone curves are presented in detail and are discussed in relation to clinico-physiological findings.

Animals↗
PubMed1990

[Cryopreservation of bovine embryos].

The method of cryopreservation of embryos aged seven days, proposed for embryo transfer with cattle by Niemann (1985), was tested under production conditions on three cattle breeding farms and three experimental animal units. The number of donors was 128, and 467 intact embryos were obtained from them and were cryopreserved in semen straw. Following thawing, 455 were recovered, and 439 (96.5 percent) of these were suitable for transfer. A pregnancy rate of 49.0 percent was recorded from 412 transfers. This rate was differentiated by oestric cycle conditions of heifer recipients, which gave percentages of 46.0 among recipients of seven-day old embryos, 45.7 for eight-day recipients, and 65.8 for six-day recipients. Related to pregnancy results recorded on the same units from transfer of freshly collected seven-day embryos, the efficiency coefficient was 0.69 (550 fresh transfers = 65.4 percent and 222 cryopreserved transfers = 48.2 percent). The method is recommended for general field practice.

Animals↗
PubMed1989

Gene transfer experiments in cattle.

Various methods for gene transfer in cattle are described. Four vectors containing (1) the bovine papilloma virus DNA, (2) the alcohol dehydrogenase gene of Drosophila melanogaster, and the human (3) and the bovine (4) growth hormone gene were used for gene injection in bovine zygotes. The period between 78 and 82 h after prostaglandin treatment was determined as the optimum time for collection of bovine zygotes. A total of 802 eggs was obtained from 74 successfully superovulated heifers. The foreign DNA was injected into the pronuclei of 156 (Construct 1), 130 (Construct 2), 62 (Construct 3) and 250 (Construct 4) centrifuged zygotes. An average number of 12 zygotes per recipient heifer was transferred into one oviduct. The eggs were recovered after 14 days (Constructs 1 and 2) for analysis or after 7 days (Constructs 3 and 4) respectively for transfer into final recipients. After the transfer into 23 recipient heifers of 43 embryos (Construct 4), 14 calves were born and 1 fetal monster was isolated. According to dot-blot hybridization the total rate of efficiency of gene transfer was 3.2%, 6.9%, and 0.8% for Constructs 1, 2 and 4, respectively.

Animals↗
PubMed1980

[Progesterone and estradiol levels in blood plasma of calves following superovulation treatment].

Studies into quantitative behaviours of oestradiol and progesterone in the blood plasma of six heads of prepuberal cattle, following superovulation treatment, using pregnant-mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG) have shown the oestradiol concentrations to double, as early as two days after PMSG, and to go up to ten times their original values, after another four days had elapsed. Progesterone values increased, as early as along with induced oestrus, and, consequently, suggested scatter of the ovulation process. The maximum progesterone levels reached something in the vicinity of 100 ng/ml, following superovulation, and, consequently, were far in excess of anything recordable from spontaneously ovulating cyclic cattle. Hormone analyses were continued over six weeks from induced oestrus and superovulation, with evidence being produced to the effect that no permanent cyclic activity had been introduced. All calves rather returned to their prepuberal condition.

Animals↗
PubMed1976

[Studies on biotechnical ovulation synchronization in young cows. 1. Ovulation periods, ovarian findings and fertilization results after use of Metallibur, 750 IE PMS and 250 or 500 IE HCG].

The following treatment was applied to 50 mature young sows in two experiments (22 and 28 animals) throughout 20 days: Turisynchron-Prämix (Turi.), 5 g/animal, and 750 IU PMS (Prolosan serum) 28 hours after Turi. In the first experiment. 250 IU HCG (Gonabion) were additionally injected to each of twelve animals 100 hours after Turi., while in the second experiment each of ten animals recieved 500 IU HCG 103 hours after Turi. The remaining animals of the two groups were used as controls. Inseminations took place 101 and 104 hours (fourth day) after Turi. in the first experiment and 125, 149, as well as 173 hours (fifth, sixth, and seventh days) after Turi. in the second. Onset of ovulation was brought forward to the sixth day after Turi. in response to 500 IU HCG by laparotomy performed in the mornings and evenings of the fourth through seventh days. Most of the controls and test animals with 250 IU HCG ovulated on the sixth or seventh day after Turi. Ovulation was stimulated by both HCG dosages, in comparison to the controls, which was established by slaughtering the animals between the seventh and twelfth days after Turi. The percentage of ovulations was higher among the test animals and that of ovarian cysts lower. Fertilisation of the second group was clearly better than that in the first where insemination had taken place two days prior to ovulation, that is too early. The latter results were secured by tubal douche and ovocyte tests.

Animals↗
PubMed1974

[Behavior of neutral 17-ketosteroids in the urine of female pigs].

Ketosteroids were determined colorimetrically in samples of 24-hour urine from sows, ten days after service. Pregnancy was diagnosed by injection of the "Gravignost-swine" preparation in eight sows. Significant differences between the 17-ketosteroid excretion on non-pregnant sows were revealed by the excretion in 24-hour urine, but not by urinary concentration expressed in mg per litre. Injection of "Gravignost" had no effect on 17-ketosteroid values. In pregnant sows the urinary concentration of 17-ketosteroids was inversely proportional to daily urine volume, and there was also a significant correlation between urine volume and 17-ketosteroid excretion expressed in mg per 24 hours. The method used for determining urinary ketosteroids was sufficiently reliable and simple to be used for routine testing.

Animals↗