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P S Karaseva

Publications and source records attributed to P S Karaseva.

17 recordsLinked to original sources
PubMed1983

[In vitro study of macrophage barrier function in mice vaccinated against tick-borne encephalitis and infection with flaviviruses of varying virulence].

Tick-borne encephalitis (TBE) and Langat viruses were shown to be equally capable of multiplication in mouse peritoneal macrophages (PM) in vitro. The reproduction dynamics of TBE virus proved to be the same in PM of mice both highly sensitive and relatively less sensitive to TBE virus. The preliminary immunization of PM donors with commercial inactivated or experimental concentrated TBE virus vaccine produced no effect on the capacity of the virus for multiplication in PM. These facts indicate the absence of correlation between the capacity of the viruses under study for multiplication in PM in vitro and their virulence in vivo, as well as the insignificant role of circulating macrophages in the realization of the barrier function in an immune or nonimmune body.

Animals↗
PubMedDate not supplied

[Dependence of immune cytolysis of cells infected with western equine encephalomyelitis virus upon the cell cycle stages].

In the first few hours after inoculation of Chinese hamster cells with western equine encephalomyelitis virus the mitotic activity of the cells did not change significantly but mitoses stopped mainly in the stage of metaphase and had all signs of pathological mitoses. Treatment of the cells for 1 hour with the homologous antiviral immune serum and complement resulted in destruction and death of practically all cells except those in the state of mitosis. In the latter specific fluorescence of virus antigen was demonstrated by the fluorescent antibody technique same as before treatment with the homologous immune serum and complement. Resistance of mitoses to immune cytolysis is probably conducive to virus persistence.

Animals↗
PubMedDate not supplied

[Immunity indices of mice immunized with different vaccines against Japanese encephalitis].

The test of adoptive transfer of immunity demonstrated that in the mice vaccinated with inactivated Japanese encephalitis vaccines the protective activity to the homologous virus is provided predominantly by B-lymphocytes the protective effect of which is enhanced by cooperative interaction with T-lymphocytes. The culture (CV) and purified brain (BV) vaccines induce high levels of humoral and cell-mediated immunity, however, the capacity of CV to produce antibody is higher than that of BV. Both preparations do not cause in vaccinated mice any changes in responsiveness to extraneous antigen (sheep red cells), or disorders in chemotaxic mobility of leukocytes and phagocytic activity of macrophages.

Animals↗
PubMedDate not supplied

[Inactivated vaccine against Japanese encephalitis. The characteristics of a purified preparation from mouse brain].

A vaccine has been prepared from formalin-inactivated suspension of brain tissue of Japanese encephalitis (JE) virus-infected white mice. The material was purified by flow ultracentrifugation in sucrose density gradient. Four members of the Nakayama antigenic group were compared as production JE virus strains for the vaccine preparation. Satisfactory results with regard to the immunogenicity of the bulk vaccine determined in mice (the intensity of response and the antigenic spectrum range) were obtained for three of them, including only one of the two variants of the Nakayama NIH strain. The highly purified experimental vaccine proved to be immunologically active and safe in trials in laboratory animals and 20 human volunteers. After two vaccinations, immunological changes in people were recorded much more frequently in the neutralization test than in the hemagglutination-inhibition test. Blasttransformation of lymphocytes in response to the specific antigen was observed in 5 out of 7 vaccines examined. There was no strict correlation of this test with the results of both serological tests.

Adult↗