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Biomedical subjects

P S Riboldi

Publications and source records attributed to P S Riboldi.

7 recordsLinked to original sources
PubMed1984

Anti-ribosomal ribonucleoprotein autoantibodies in systemic lupus erythematosus.

Sera from systemic lupus erythematosus (SLE) patients giving a fluorescent ribosomal pattern on tissue and cell preparations also showed precipitating autoantibodies against purified rat liver ribosomes. Ribosomal antigen is also present in rabbit thymus cellular extract (RTE), since the same sera gave precipitin lines against RTE in identity with ribosomes. Immunofluorescent staining was completely inhibited by serum absorption with ribosomes or with RTE. However ribosomal RNA and RNase or trypsin-treated ribosomes failed to react with these autoantibodies as demonstrated in immunoabsorption and immunodiffusion studies. These data suggest that these sera contain autoantibodies directed against some antigenic site composed of a portion of both RNA and ribosomal protein. Ribosomal autoantibodies were detectable at a low frequency in SLE patients characterized by an active disease and renal involvement.

Antigens↗
PubMed1984

Neutrophil chemotactic factor of anaphylaxis (NCF-A) release in aspirin-induced asthma.

Neutrophil chemotactic activity (NCA) following oral challenge with aspirin (ASA) was determined in ASA-intolerant asthmatic subjects, and in ASA-tolerant asthmatic and normal subjects. There was a statistically significant fall in FEV1 and a rise in NCA (P less than 0.01) following challenge in the ASA-sensitive subjects compared with that of the ASA-tolerant subjects and normal controls. No significant difference was observed between the latter two groups. The chemotactic factor identified in this study had a molecular weight greater than 150 000 which is consistent with NCF-A (neutrophil chemotactic factor of anaphylaxis). The ASA-induced fall in FEV1 and rise in NCA was further studied in three of the ASA-intolerant asthmatic subjects, with and without pretreatment with inhaled sodium cromoglycate. In these subjects, the drug inhibited both the oral ASA-induced bronchoconstriction and the increase in neutrophil chemotactic activity. These results suggest that ASA-induced asthma involves mediator release from mast cells, as shown by the increase in NCA following ASA challenge and the protective effect of sodium cromoglycate which is considered to inhibit mast-cell degranulation.

Adult↗
PubMed1982

Phagocytic function of polymorphonuclear leukocytes in essential mixed cryoglobulinaemia.

We have studied the phagocytic function of polymorphonuclear leukocytes obtained from 16 patients with essential mixed cryoglobulinaemia and 16 normal subjects, utilizing three different experimental models. The in vitro phagocytosis of Candida albicans by polymorphonuclear leukocytes obtained from the patients in the presence of autologous serum was subnormal as compared with controls (p less than 0.002). The neutrophil defect appeared to be cellular since: a) no effect of the patient's serum on the phagocytic capacity of normal neutrophils was demonstrable; b) the defect was not reversed when normal AB serum was used as opsonin source. The polymorphonuclear leukocytes from the same patients ingested IgG-coated sheep red blood cells to a significant higher degree than normals (p less than 0.002) when tested in patient's serum free-medium. Finally, no differences between patients and controls were observed on the basis of latex uptake. A possible explanation for these contradictory results is discussed.

Adult↗
PubMed1977

Anti-gamma-globulin activity, DNA and antibodies to DNA in nonlupoind cryoglobulinemias.

Anti-gamma-globulin activity, free DNA, and DNA binding were studied in 19 cryoglobulinemias: 8 patients with Waldenström's disease (WD), 9 patients with essential cryoglobulinemia (EC), and 2 with chronic aggressive hepatitis (CAH). Antihuman gamma-globulin activity was detected in all sera and dissolved cryoprecipitates but two from EC and one from the CAH group. By diphenylamine assay we found DNA in two sera from WD and in one serum and cryoprecipitate from EC. An antibody to denaturated DNA was shown only in sera from the two patients with CAH and from one patient with EC. Nonspecific binding was more frequent.

Antibodies, Anti-Idiotypic↗
PubMed1975

[Immune complexes DNA-anti-DNA during the course of mixed cryoglobulinemia].

Plasma and isolated cryoglobulins from ten patients with mixed cryoglobulinemia were studied for the presence of DNA using the diphenylamine reagent test. DNA-like material was detected in all the plasmas tested and it was also present in nine of the isolated cryoglobulins. IgM and IgG components of three cryoglobulins were purified by gel-filtration: anti-DNA activity was detected in IgG fraction (two cases), in IgM and IgG (one case); antigammaglobulin activity was always found in IgM fraction.

Antibodies, Anti-Idiotypic↗
PubMed1975

Anti-IgA antibodies in two brothers with selective serum IgA deficiency.

A selective serum IgA deficiency with anti-IgA antibodies at high titer were found in a patient who suffered severe transfusion reactions. The same abnormalities were detected in his brother, a 72-year-old-man in good general condition. Anti-IgA antibodies were found in the IgG fraction and were directed against alpha-chains. A deficiency of isoagglutinins and an absence of heteroagglutinins in the propositus' serum could be shown.

Aged↗