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P SADHUKHAN

Publications and source records attributed to P SADHUKHAN.

5 recordsLinked to original sources
PubMed1964

QUALITY CHANGES IN VACCINIA VIRUS DURING ADAPTATION TO GROWTH IN CULTURES OF EARLE'S L CELLS.

Sharp, D. G. (University of North Carolina School of Medicine, Chapel Hill), P. Sadhukhan, and G. J. Galasso. Quality changes in vaccinia virus during adaptation to growth in cultures of Earle's L cells. J. Bacteriol. 88:309-312. 1964.-Although particle production improves rapidly with passage of vaccinia (mouse neurotropic strain) virus in L cells, plaque production improves much more slowly. Beyond the passage when maximal particle yield was reached (18th), further improvement in plaquing quality of 1,000-fold was observed. Particle count and plaque titrations showed that plaquing efficiency improves at comparable rates in either plaque-to-plaque passage on plates or in tube cultures inoculated at high multiplicity. The host cell is probably exerting some influence in addition to that of the selection of mutants from the initial population.

Animals↗
PubMed1961

Electron microscope studies on the haemoglobin molecules.

Haemoglobin molecules isolated from normal human subjects have been directly micrographed under the electron microscope following in general Hall's technique. The average height (h) and the widths along (w(11)) and perpendicular (w( perpendicular)) to the shadow direction of the molecules have been measured as 56.5 +/- 6.6 A, 122.7 +/- 15 A, and 120.9 +/- 20 A, respectively. The exaggeration in the molecular widths due to the deposition of metal cap ranges between 60 to 70 A. The probable resolution of the substructure of the molecule, e.g., presence of "holes" and dimples, in the present electron microscopic evidence has been discussed. The electron microscopic results on the size of the individual haemoglobin molecules are in satisfactory agreement with the recent x-ray diffraction model of Perutz and his associates for horse haemoglobin.

Animals↗