Immunohistochemical quantification of steroid receptors and other prognosis factors in human breast cancer patients.
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Biomedical subjects
Publications and source records attributed to P Schwarzmann.
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The paper introduces telemicroscopy as a mean to establish online telepathology services. It is differentiated between specialized point to point connections with only a few restrictions on one side, and telecommunication links available inexpensive worldwide in the form of the public telephone net on the other side with the restriction of a limited channel capacity. The restrictions imposed by the application of the telephone net (preferentially the ISDN service) are discussed and strategies outlined to overcome these restrictions. It is concluded, that if dedicated broadband connections are available, realtime telemicroscopy links can be realized immediately with available equipment. If a switched and general available telemicroscopy service applying public telecommunication links is favoured, for effective and acceptable ("online") links, computer intelligence has to be introduced to allow effective strategies for data reduction and data transmission. In this field design and development efforts are still necessary.
Effective telepathology is only possible, if the partners are supplied by a broad variety of equipment connected by far-spread communication lines. These prerequisites can only be fulfilled if technical and medical standards are available obligate for all participants. The technical and medical aspects of those standards are described in general and include procedures and notations in pathology, standards of the image source, image sampling, clinical information, classification of diagnosis, communication channels, hard- and software equipment. New standards should only be developed for those not existing, or if the already existing ones are not appropriate. The standardization classes should allow flexible formats and be developed as "open modules" allowing "individual" peripheral work stations and progressive development in the future.
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We describe three different methods of calibration of quantitative immunohistochemistry, two of them based on self-made dye-agarose or antigen-agarose substrates. Quantification of immunostained objects (immunosignals) can be done by (1) microscope photometry (plug method) or scanning-photometry; (2) and densitometry of a television transformed microscope image. Both approaches are subjected to the different methods of calibration and three examples of application.
A new procedure for the conversion of binary black and white images to a tactile presentation perceptible by the blind is described. Conventional procedures are very time-consuming and expensive and are not adapted to produce the required low editions of tactile displays, e.g., for schools for the blind or individual applications. The procedure recently developed allows the fast, economical conversion of even single drawings to a tactile display with high resolution. An account of the investigations and valuations of all possible physical procedures of optical-to-tactile conversion is given. The new procedure, based on a slightly modified Xerox copying process in a first step and gumming tactile material with the tone powder at the darkened regions in a second step, is described in detail. The device realised is presented. It allows the conversion of one page of a drawing to a tactile display with a resolution of 0.3 mm within 1 min. Various applications of the system and experiences with blind users are reported. The implement proved to meet most of the requirements of blind people. It is easy to handle and produces tactile drawings of excellent quality. As it is based on commercial subsystems it promises an economical production even at small series.
This paper presents preliminary results of a cell classification experiment using a new approach for feature extraction. The algorithm takes into account the special requirements of a fast parallel processing system (processor-oriented algorithms). A cell image is described by several hundred features derived from the nucleus only. The most significant features with respect to classification are determined by statistical analysis. Applying principal axis transform, a new feature set is computed, reduced considerably in dimensions. The data base (1,925 cell images of Papanicolaou-stained cervical specimens) was divided into a training set (963 images) and a test set (962 images). The classification results of the test set show that the recognition rate for the two-class problem (normal, suspicious) is better than 91%, using only ten morphologic features.