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Biomedical subjects

P Sewell

Publications and source records attributed to P Sewell.

7 recordsLinked to original sources
PubMed2005

A photoelastic clinical study of the static load distribution at the stump/socket interface of PTB sockets.

It is recognized that the assessment of prosthetic socket fit is based largely on the subjective clinical judgement of the prosthetist. This study assesses a novel technique, photoelasticity, for use as a tool for the qualitative and quantitative assessment of socket fit. Photoelasticity is a visual technique that produces contours of principal stress or strain differences. The colour and/or distance between the contours can be qualitatively or quantitatively assessed, using a polariscope, to give a full-field analysis of the stresses on the sockets's surface. This paper presents qualitative photoelastic socket surface contour data gathered during several prosthesis fitting sessions for two male trans-tibial amputees. Results are compared with the actual known contact regions at the stump/socket interface to determine if a relationship exists. This comparison of results has then been used to conclude the suitability of photoelasticity as a tool for the assessment of socket fit and recommendations are made as to the future developments of the technique. A direct relationship between the stump/socket contact regions and the qualitative photoelastic contours was demonstrated. Given further development this photoelastic technique may therefore be suitable for qualitative analysis of the interactions between the stump and prosthetic socket.

Aged↗
PubMed2000

Developments in the trans-tibial prosthetic socket fitting process: a review of past and present research.

A revolution in trans-tibial prosthetic design began at the end of World War II with the development of new materials and a dramatic improvement in the understanding of biomechanics. Early research was based mainly on the improvement of existing prosthetic design practice. Today, research has been focused on providing a better understanding of stump/socket interface biomechanics and improving socket fit by attempting to quantify the normal/direct stresses at the interface. The purpose of this review paper is to question whether research and prosthetic education/training to date has significantly improved our understanding of what makes a good socket. Although there is no doubt that advances in socket fitting techniques have been made what is not clear is the actual extent to which these advances have improved the quality of sockets fitted. It is suggested that a new approach is needed which can overcome some of the inherent problems of designing and manufacturing a comfortable high quality socket. It is also suggested that current research and education/training in the fields of pressure/interfacial interaction measurement and Finite Element Analysis techniques have limited potential to address many of these problems. There is also little evidence that current computer aided design systems offer any significant advantages over more conventional techniques.

Amputation, Surgical↗
PubMed1998

Transgenic nematodes as biomonitors of microwave-induced stress.

Transgenic nematodes (Caenorhabditis elegans strain PC72), carrying a stress-inducible reporter gene (Escherichia coli beta-galactosidase) under the control of a C. elegans hsp16 heat-shock promoter, have been used to monitor toxicant responses both in water and soil. Because these transgenic nematodes respond both to heat and toxic chemicals by synthesising an easily detectable reporter product, they afford a useful preliminary screen for stress responses (whether thermal or non-thermal) induced by microwave radiation or other electromagnetic fields. We have used a transverse electromagnetic (TEM) cell fed from one end by a source and terminated at the other end by a matched load. Most studies were conducted using a frequency of 750 MHz, at a nominal power setting of 27 dBm. The TEM cell was held in an incubator at 25 degrees C inside a shielded room; corresponding controls were shielded and placed in the same 25 degrees C incubator; additional baseline controls were held at 15 degrees C (worm growth temperature). Stress responses were measured in terms of beta-galactosidase (reporter) induction above control levels. The time-course of response to continuous microwave radiation showed significant differences from 25 degrees C controls both at 2 and 16 h, but not at 4 or 8 h. Using a 5 x 5 multiwell plate array exposed for 2 h, the 25 microwaved samples showed highly significant responses compared with a similar control array. The wells most strongly affected were those in the rows closest to the source, whereas the most distant row did not rise above control levels, suggesting a shadow effect. These differential responses are difficult to reconcile with general heating effects, although localised power absorption affords a possible explanation. Experiments in which the frequency and/or power settings were varied suggested a greater response at 21 than at 27 dBm, both at 750 and 300 MHz, although extremely variable responses were observed at 24 dBm and 750 MHz. Thus, lower power levels tended, if anything, to induce larger responses (with the above-mentioned exception), which is opposite to the trend anticipated for any simple heating effect. These results are reproducible and data acquisition is both rapid and simple. The evidence accrued to date suggests that microwave radiation causes measurable stress to transgenic nematodes, presumably reflecting increased levels of protein damage within cells (the common signal thought to trigger hsp gene induction). The response levels observed are comparable to those observed with moderate concentrations (ppm) of metal ions such as Zn2+ and Cu2+. We conclude that this approach deserves further and more detailed investigation, but that it has already demonstrated clear biological effects of microwave radiation in terms of the activation of cellular stress responses (hsp gene induction).

Animals↗
PubMed1993

Total numbers of neurons in myenteric ganglia of the guinea-pig small intestine.

Two techniques that are thought to stain all of the neurons in the myenteric ganglia of the intestine are NADH diaphorase histochemistry and immunohistochemistry using a "nerve cell body" antiserum. However, this assumption has never been directly verified. In the present study myenteric ganglia of the guinea-pig ileum were prepared as whole-mounts and stained with either of these techniques. All nerve cells that could be identified in the whole-mounts were counted. The whole-mounts were then embedded flat in resin and serially sectioned at 1 micron. Nerve cells were identified and counted from the serial sections, and the data compared to those obtained from the whole-mounts. NADH diaphorase histochemistry did not reveal all the neurons at incubation times that gave selective staining. In contrast, "nerve cell body" antiserum stained the entire neuronal population. To determine the total number of nerve cell bodies/ganglion and the proportion of nerve cell bodies with calbindin immunoreactivity, whole-mounts that had been processed for calbindin immunohistochemistry were serially sectioned and reconstructed. The total number of neurons per myenteric ganglion was 105 +/- 10 (SE). Calbindin-immunoreactive neurons comprised about 20% of the myenteric neurons, which is considerably less than previous estimates, because previously the total population has been underestimated. The spatial density of myenteric neurons in the undistended ileum of the guinea-pig is 17,300 nerve cells/cm2.

Animals↗