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P Solisch

Publications and source records attributed to P Solisch.

At least 19 recordsLinked to original sources
PubMed1992

Some physicochemical properties of the virus of rabbit haemorrhagic disease.

Purified and concentrated preparations of virus from liver extracts of infected rabbits contain virus specific components with sedimentation coefficients of about 175, 110 and sometimes 133S and more slow units. Full and empty virus particles with a diameter of about 34 nm were shown electron microscopically in the corresponding 175 and 110S fractions of the sucrose density gradient. The average of buoyant density of the 175, 133, 110S and more slow units are 1.36, 1.32 and 1.31 g/ml respectively. The extinction coefficient E260 nm is 4.3 +/- 0.7 cm2/mg. The RNA content is 17 +/- 4%. SDS-PAGE shows a "65" kD protein as a single or major component. Beside smaller polypeptides with lower intensities, the 67 kD polypeptide reacts positively in the Western blot with polyclonal antibodies of rabbits. The molecular weight of the virus is 15 +/- 4 x 10(6)D. The pH stability of the 175S unit was also tested.

Animals↗
PubMed1992

[The importance of an as yet unknown cell reaction: "RIV." A hypothesis].

The research findings described in this paper led to the definition of a new notion, "Reaction Pattern in Vertebrate Cells" (RiV) and, by means of a hypothesis, made it possible to submit a proposition on RiV valency. The problem is of practical importance, particularly for assessment and control of virus infections and tumour diseases.

Animals↗
PubMed1989

[Retroviruses. Problems in electron microscopic diagnosis].

A department of electron microscopy with specialisation in virus diagnosis evaluates the results concerning retrovirus findings. In the course of research work already classified retroviruses were observed, numerous unexpected retrovirus diagnoses being of special interest. This concerned exclusively to ultra-thin sections of permanent and immune cells. Expounded in this paper are principles by which examiners should abide in any case. Reference is also made to the importance of individual findings.

Animals↗
PubMed1987

[A hitherto unknown reaction pattern in vertebrate cells (RiV). 4. Ultrastructure of formation and mechanism of action of RiV in cultured cells].

Ultrastructural investigations show RiV to be a secretory process for the cell and tissue. The following functions are attributed to it: 1. Release of information flow in the direction of the cell membrane. 2. Formation of extracellular or paracellular matrix structures as a part of the morphological equivalent of the postulated cellular resistance phenomenon.

Animals↗
PubMed1986

[A hitherto unknown reaction pattern in vertebrate cells (RiV). 2. The protective effect of RiV particle preparations against foot-and-mouth disease in guinea pigs].

Further observations concerning the previously described RiV-particles are reported. They were isolated from a diploid cell line of bovine origin, embryonal duck fibroblasts and BHK-21 cells. A protective effect against foot-and-mouth-disease virus in guinea pigs could be observed following inoculation with the RiV-preparation of bovine origin. All 3 preparations isolated from the 3 cell lines showed immunologic cross reactions.

Animals↗
PubMed1986

[Hitherto unknown pattern of reaction in vertebrate cells--ultrastructural studies of virus-host cell relationship and cell cultures. 1].

A hitherto unknown pattern of reaction has been observed in diverse ultracellular analyses involving 111 different scientific and diagnostic studies of virus-host cell relationships and cell culture studies. The essence of this reaction was the presence of spherical 50 nm particles which could be distinguished from known structures and cellular inclusions and in particular from viral particles. RiV is associated with viral infection in vivo und in vitro, with cell cultures of embryonal or fetal origin and with a large number of passages by permanent cell lines. RiV may be related to the phenomenon of resistance.

Animals↗
PubMed1985

Electron microscopic investigation on the fine structure of the virus of enzootic bovine leukosis (BLV).

Systematically electron microscopical control of BLV producing cells and stimulated lymphocytes of leukotic cattle in the last years revealed maturation processes of BLV in a different form from comparable type C viruses. Electron microscopic representation of bridge-like junctions between core and envelope within examined retroviruses were discussed and compared with new model concepts.

Animals↗
PubMed1981

[Structure of the enzootic cattle leukosis virus. 1. Biophysicochemical characterization of several virus-specific components].

Concentrated virus material partially purified, after having been obtained from permanent cell cultures, was allowed to undergo repeated gradient centrifugation for an analysis of density and sedimentation. Serological tests were conducted, in that context, together with electron microscopy and gel electrophoresis. Isodensities of the virion were 1.17 g/ml in CsCl solution or 1.16 g/ml in saccharose solution. The average densities of the internal bodies, between 45 nm and 80 nm in diameter, were measured by electron microscopy and accounted for something between 1.32 g/ml and 1.34 g/ml in CsCl solution or between 1.22 g/ml and 1.24 g/ml up to 1.28 g/ml in saccharose solution. Four revertase-positive components, 915 S, 704 S, 469 S and 264 S +/- 40 S, were found in flattened cane-sugar gradients. conclusions for non-invasive ultracentrifugation in the virus were drawn from those high S-values. High-titre mixed antigens, virus-specific bands from the density gradients, and washed antigen-antibody precipitates were investigated by the authors by means of slab-gel electrophoresis. Molecular weights of 53 kilodalton (kD) and 24 kD were recorded from the major components. There also were polypeptides weighing 10 kD, 12 kD, 15.6 kD, 42.5 kD, 63 kD, and 70 kD.

Animals↗
PubMed1981

[Morphology of coronaviruses-- electron microscopic demonstration by negative contrast technic of the transmissible gastroenteritis virus of swine].

This contribution to the morphology of corona virus is based on the use of electron microscopy in a negative contrast investigation of the virus of transmissible gastroenteritis of swine. Viruses of transmissible gastro-enteritis are spherical or, in rare cases, pleomorphous particles of typical corona virus structure. The viruses investigated by the authors were 132 nm in diameter. The surface projections, consisting of a flattened knob and conical shaft, are 18 nm in length. Internal bodies, 50 +/- 2 nm in diameter, occur as "filled" or "empty" particles. The thickness of all internal bodies and virus membranes is 8 +/- 1 nm. Differences were found to exist between virus of transmissible gastro-enteritis and particles similar to corona virus and isolated from faecal samples. A first comparison had been made for that purpose. Those differences were relating to shape and size of individual particles and to morphological setup of surface projections.

Animals↗