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Biomedical subjects

P T Chandler

Publications and source records attributed to P T Chandler.

At least 19 recordsLinked to original sources
PubMed2000

A method to analyze production responses in dairy herds.

Milk production was simulated in a 50-cow herd averaging 8182 kg of 305-d milk with a standard deviation of 1364 kg. Herd demographics were 35% first lactation, 20% second lactation, and 45% third or greater lactation cows. A lactation model was developed with the Wood's equation (Milk/d = A*DIM*e(-c*dim)) to which random variation was added to be consistent with a coefficient of variation of 10% for daily milk production. Five sequential sampling periods, 30 d apart, were randomly selected for the experiment. For each of these sampling periods data were simulated for cow, lactation number, milk, and days in milk (DIM). To the third sampling period, a known input was pulsed into each cow record to simulate a change in milk production. Inputs and number of herds simulated were -1.140 kg and 15 herds, 0.909 kg and 30 herds, -0.455 kg and 20 herds, 0 kg and 65 herds, 0.455 kg and 21 herds, -0.909 kg and 47 herds, 1.140 kg and 20 herds, and 2.270 kg and 15 herds. Regression by cow was used to estimate milk production change for the known inputs: Milk(ijk) = Intercept + beta(i)*DIM(ij) + TRT(ik) + epsilon(ijk). Parameter estimates for each cow were submitted to analysis of variance with herd as a class variable. The least square mean of TRT (dummy variable for known input of milk volume change) for herd was tested for difference from zero based on a "t" statistic. Herd responses were classed as negative, not different from zero, and greater than zero based on P < 0.10. Herd responses were categorized based on the known input to assess the ability of the method to detect a change in production. The mean estimate of TRT from the regression analysis was used to assess the ability of the method to estimate the magnitude of the known input. The regression method was able to detect changes in production greater than 0.455 kg, but is more useful when changes of 0.9 kg or greater are shown. Adjustment for days postcalving on first test day is necessary to correct for the bias in linear regression to estimate response across the curvilinear milk production function.

Animals↗
PubMed2000

Effects of inclusion of a blended protein product in 35 dairy herds in five regions of the country.

We assessed the change in milk production in 35 dairy herds located in eight states when a blended animal-marine protein product was included in diets at 2 (22 herds) to 4% (13 herds) of dry matter. Average 305-d production across herds was 8844.1 kg (SEM = 153.7 kg) of milk, with a range of 6876.9 to 11,293.2 kg. The mean days in milk for all herds at initiation of the trial was 118.8 d (SEM = 3.7, herd mean range of 68.8 to 160.0 d), and the average daily milk production was 32.6 kg (SEM = 0.6 kg, range 24.2 to 39.6 kg). The animal-marine protein blend was included in herd rations for 30 d, beginning immediately after a DHI herd test month and ending immediately after the next DHI sample test. Cow milk records were collected for 1 to 2 mo before the protein blend was included and for 2 to 3 mo after the protein was removed. Sample days were assigned a dummy variable to indicate months off or on the animal-marine protein blend. A total of 33,190 milk records from 7135 cows were analyzed. The numbers within herd ranged from 35 to 2012 cows. Of the 35 herds, 19 were classed as having increased milk yield, 12 herds as having no change, and 4 herds as having decreased milk yield when the animal-marine protein blend was included in the diet. The population mean for change in milk yield with the inclusion of the animal-marine protein blend was 1.24 kg/d of milk (SEM = 0.05 kg). There was no significant effect of parity on mean response. Milk protein content was not influenced by animal-marine protein blend inclusion. Fat content was lower for the month on which the animal-marine protein blend was fed (3.51%) compared with the month prior (3.63%) and the month after (3.70%), respectively (SEM = 0.032). Stage of lactation influenced the method for calculating the production response and the actual response to the animal-marine protein blend.

Animal Feed↗
PubMed1981

Outpatient self-management of severe diabetes.

We initiated a five-point program to decrease acute and chronic complications in 25 unstable, insulin-dependent diabetics (study group A). The five points were home monitoring of blood glucose, use of multiple injections of a complex insulin mixture, strict dietary control with high protein feedings, carbohydrate loading before exercise, and use of purified insulin preparations. There was an 80% decline in detected hypoglycemic reactions over the succeeding six months. The hospitalization rate fell from 3.4 times per year to 0.64 times a year. An additional five extremely unstable diabetic patients (study group B) were treated with the same program except for the use of an open-loop continuous infusion system for insulin. Although results in this group were favorable in terms of improving glucose homeostasis, mechanical, physical, and psychologic problems can limit usefulness current devices.

Adolescent↗
PubMed1980

Extracellular amino acid effects on milk protein synthesis and intracellular amino acid pools with bovine mammary cells in culture.

Increases in free intracellular amino acids are associated with increased protein synthesis. Responses in synthesis of beta-casein and beta-lactoglobulin and of intracellular amino acid pools to graded concentrations of amino acids in the medium were observed. Mammary tissue from two Holstein cows was dispersed and cultured for 18 h with Eagle's minimal essential medium containing 1, 3, 5, or 7 times the basic concentration of amino acids. Synthesis of beta-casein and beta-lactoglobulin increased with concentration of amino acids in the medium. Intracellular pools of amino acids not provided by the medium (serine, proline, alanine, glycine, glutamic acid, ornithine) generally decreased with increased concentrations of amino acids in the medium, the exception being ornithine which increased with extracellular amino acids. Lysine, histidine, tyrosine, leucine, valine, phenylalanine, isoleucine, arginine, and threonine intracellular pools increased with increased amino acids in medium. Intracellular cystine responded with a small linear increase to increased amino acids in the medium, while intracellular tryptophan and methionine did not respond, indicating potential limitations from these amino acids.

Amino Acids↗
PubMed1978

Limiting amino acids for milk protein synthesis by bovine mammary cells in culture.

Experiments are presented to determine the effect of labeled precursor amino acid on synthesis of beta-lactoglobulin and beta-casein and to identify the most limiting amino acids in Eagle's minimal essential medium for synthesis of milk protein. Protein synthesis was measured by incorporation of carbon-14 labeled amino acids into milk protein from bovine mammary cell culture. Mammary tissue from 13 Holsteins (12.7 kg milk/day) was dispersed and cultured for 18 h. In Experiment 1, three replications (cows) with three amino acid mixtures, utilizing labeled lysine or leucine as the marker amino acid, showed that labeled lysine overestimated the effect of medium lysine, and labeled leucine overstimated the effect of leucine on beta-lactoglobulin and beta-casein synthesis. Subsequently Experiment 2 utilized 10 replications (cows) to determine the effect of a three-fold increase of each of 13 amino acids in Eagle's medium. Increased threonine or methionine improved beta-lactoglobulin synthesis and cystine increased beta-casein. Second and third best response in beta-casein were from addition of methionine and threonine. Combined analysis from 10 cows and two protein responses by multivariate procedures identified, in order of decreasing magnitude, cystine, threonine, and methionine as limiting amino acids in the dispersed cell culture.

Amino Acids↗
PubMed1977

Increased serum calcium levels induced by furosemide.

Thirteen male patients were treated with furosemide at a dosage of 40 mg by mouth daily for three weeks. Atomic absorption serum calcium determinations before and after treatment revealed a consistent elevation of the serum calcium level. It is suggested that at low dosages, furosemide is likely to raise the serum calcium level, while at daily dosages greater than 60 mg given orally, furosemide may depress the serum calcium level because of urinary losses.

Calcium↗
PubMed1977

Addison's disease secondary to North American blastomycosis.

Reported is the fifth known case of Addison's disease due to adrenal involvement by North American blastomycosis (Blastomyces dermatitidis infection). The fact that amphotericin B therapy precipitated an addisonian crisis suggests that patients with disseminated blastomycosis should have their adrenal function and reserve evaluated before undergoing systemic chemotherapy.

Addison Disease↗
PubMed1977

An update on reactive hypoglycemia.

Reactive hypoglycemia usually occurs in response to a carbohydrate load, rather than presenting as fasting hypoglycemia. The clinical picture may be that of excess circulating epinephrine or decreased cerebral cortical function. The principal causes are alimentary, "functional" (a debatable diagnosis) or early diabetes mellitus. The diagnosis is confirmed by simultaneous serum insulin and blood glucose levels and a five-hour oral glucose tolerance test. Simple management regimens are tailored to each type of hypoglycemia.

Antibody Formation↗
PubMed1976

Response to labeled precursor amino acids, varying cell density, and graded amino acid complement for protein synthesis in mammary cell culture.

Effect of labeled precursor amino acids, varying cell densities, graded quantities of amino acid complement, and incubation environment on milk protein synthesis were studied with cultures of mammary cells isolated from Sprague Dawley rats. The essential amino acid complement of Eagle's minimal essential medium was used as base. Protein synthesis, measured by incorporation of labeled lysine, leucine, and phenylalanine, was affected by source of label for the "beta-lactoglobulin fraction" and beta-casein but not alpha-lactalbumin. Cell numbers between 6 X 106 and 6 X 107 per 5 ml of culture medium did not significantly alter rates of synthesis. Increasing amounts of amino acid concentration from one to three-fold increased synthesis of "beta-lactoglobulin fraction" and alpha-lactalbumin regardless of cell population. Response to addition of essential amino acid for "beta-lactoglobulin fraction" synthesis was linear over one to five-fold with 30.9 mug/flask per fold addition (linear regression coefficient; squared correlation = .91). Results were similar for beta-casein synthesis; 25.9 mug/flask and squared correlation = .91. No culturing effects between carbon dioxide and conventional incubators were significant.

Amino Acids, Essential↗
PubMed1976

Limiting amino acid for protein synthesis with mammary cells in tissue culture.

To identify the limiting amino acid in the minimal essential medium as published by Eagle (Science 130:432, 1959) for milk protein synthesis in rat mammary cells in tissue culture, two different experimental approaches were used. The first study involved the reduction of amino acids singly from the total amino acid complement of the medium for milk protein synthesis. The second study was to investigate the effect on milk protein synthesis of single amino acid addition to the basic complement of amino acids. Order of limiting amino acids was lysine (first) and possible methionine, valine, or arginine (second).

Amino Acids↗
PubMed1976

Optimum amino acid complement for protein synthesis by rat mammary cells in tissue culture.

The amino acid requirement of rat mammary cells for milk protein synthesis was investigated in dispersed cell culture. A three-dimensional central composite design utilizing three variables (X1 = lysine; X2= methionine, valine, and arginine; X3 = isoleucine, tryptophan, threonine, phenylalanine, and histidine) at five concentrations each, was duplicated twice with mammary cells from lactating Sprague-Dawley rats. The optimum combination of amino acids for maximum milk protein synthesis from multiple regression models was X1 15.0-, X2 4.5-, and X3 1.5-fold their quantities in Eagle's minimal essential medium with leucine, tyrosine, cystine, and glutamine at the base 1-fold in the medium.

Amino Acids↗
PubMed1976

Protein and methionine hydroxy analog for lactating cows.

A 2 X 2 factorial experiment (protein 12.5 and 15.5; methionine hydroxy analog 0 and .125% dry matter) included 144 cows for one complete lactation, distributed over seven locations. Rations were formulated to the desired protein, methionine analog, and constant amounts of fiber 17%, sulfur .225%, calcium .6%, phosphorus .4%, and salt .5%. Treatment effects were not apparent for dry matter intake, daily milk and fat-corrected milk production, conversion of energy, and body weight changes. Conversion of dietary crude protein into milk protein was 34.5% for the low and 25.8% for the high protein ration. Methionine analon (0% = 2.54; .125% = 1.90). Effect of methionine analog was most apparent at low protein as 0 analog cows produced 247 kg fat, required 2.9 services/contraception, and had 156 days open whereas cows on other treatments (.125% analog and/or high protein) produced 264 kg fat, required 1.8 to 2.2 services/conception, and had 124 to 134 days open. Methionine analog response is discussed in relation to tuminal and postruminal effects as well as the interrelation with protein and energy.

Animals↗