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Biomedical subjects

P Thiele

Publications and source records attributed to P Thiele.

At least 19 recordsLinked to original sources
PubMed1996

Signal transduction of erythropoietin in endothelial cells.

Erythropoietin (EPO) induces endothelin expression in endothelial cells (EC) and has angiogenic effects. We investigated the intracellular signal transduction of EPO in EC and tested the hypothesis that the proliferative effects of EPO may be mediated by cytosolic calcium, changes in intracellular pH, or tyrosine phosphorylation. Cytosolic calcium and pH were measured with fura-2 and BCECF. Protein phosphorylation was assessed with 32P-labeled EC and two-dimensional (2D) gel chromatography. Tyrosine phosphorylation was measured using specific antityrosine antibodies and confocal microscopy. Proliferation was measured by thymidine incorporation and cell count. No effects of EPO on cytosolic calcium and pH were observed. In contrast, erythropoietin increased phosphorylation of 94, 70, 42, 40, 29 and 25 kDa proteins at five minutes and 60 minutes. Most of the early proteins were tyrosine phosphorylated. Confocal microscopy showed cytosolic as well as membrane-bound tyrosine phosphorylation in resting cells and an EPO-induced translocation of immunoreactivity to the nucleus. Immunostaining for the transcription factor STAT-5 showed that EPO induced a nuclear translocation of STAT-5. EPO 0.5, 2, and 4 U/ml increased proliferation, an effect that was prevented by incubation with the tyrosine kinase inhibitor genistein. We conclude that EPO induces proliferation in EC initially via tyrosine phosphorylation of six distinct proteins, and that the phosphorylation and nuclear translocation of the transcription factor STAT-5 is important for the effects of EPO on EC.

Biological Transport, Active↗
PubMed1990

[Expert assessment of chronic venous insufficiency].

In the expertise of a chronic venous insufficiency kind and stage of the venous disease must be proved as objectively as possible. The diagnostic demands in the individual step programmes serve for the exact assessment of the remaining part of efficiency. The criteria of valuation are summarized, in which case we also deal with several possibilities of combination with other vascular diseases. The expert opinion of an accident is discussed. References to the inability to work, rating of nursing money, increased material benefit in impairments of health and to the acknowledgement of an identity card for injured persons supplement the statements.

Disability Evaluation↗
PubMed1987

[Current therapy of chronic arterial occlusive diseases of the lower extremities].

Angiologists, vascular surgeons, and angiographers inform about the actual state of the therapy of the arterial occlusive diseases of the legs. The degree I of therapy comprises the conservative treatment for the improvement of the macrocirculation and the normalisation of the disturbed microcirculation, taking into particular consideration the therapy with antiviscositant drugs. The main chapter ends with the possibilities of the influence on the arteriosclerotic vascular processes as well as with the necessary rehabilitative measures. In the paragraph about the degree II of therapy the indications, contraindications, methods and results of the isolated and combined percutaneous transluminal angioplasty, the systemic and local fibrinolysis as well as the reconstructive vascular operations are represented.

Arterial Occlusive Diseases↗
PubMed1987

[Whole blood viscosity in patients with acral arterial occlusive diseases and their modification by rheologically active drugs].

Measurements of the whole-blood viscosity in patients with organic and functional disturbances of the acral blood supply were performed. In comparison with the whole-blood viscosity in normal persons patients with acral occlusive diseases showed a significantly increased viscosity. On the other hand, in patients with functional disturbances of the acral blood supply no differences could be established. In a therapy study the good efficacy of rheologically effective medicaments could be demonstrated particularly as combination therapy of pentoxifylline, acetylsalicylic acid and tolazoline in patients with acral occlusive diseases. These medicaments should by all means be used together with the basic therapy. In functional disturbances of the blood supply should predominantly be maintained physiotherapeutic methods of treatment.

Adult↗
PubMed1984

[Theses on the evaluation of arterial circulatory disorders of the extremities].

In an expert opinion of disturbance of the arterial blood supply concerning invalidity the vascular disease must be proved with sufficient ascertainment as well as the functional capacity remained must be estimated. Every expert opinion should contain as far as possible concrete data already in the angiologic-diagnostic minimum programme. In problematic cases supplementations by a special angiological diagnostics are necessary. The criteria of evaluation of arterial obstructive diseases for an expert opinion concerning invalidity are also summarized in theses. Also the relations between disturbances of the peripheral blood supply and effects of injury are discussed. The paper concludes with references to the acknowledgement of a disability card in arterial obstructive diseases and to the motor-car driving ability of patients with vascular diseases.

Angiography↗
PubMed1983

[Significance and taxonomy of functional and organic acral circulatory disorders and cutaneous microangiopathies].

Acral disturbances of the blood supply and cutaneous microangiopathies may be associated with functional as well as with organic vascular disturbances. The primary angiopathies are independent clinical pictures, the causes of which are still unknown. In numerous diseases secondary forms of the disturbances of the acral blood supply and the microangiopathies can occur. The systematics represented corresponds to the relevant ambulatory view-points. On the basis of the frequency of several clinical pictures is referred to the importance for the policlinical practice.

Cardiovascular Diseases↗
PubMed1983

[Clinical aspects and differential diagnosis of primary acral circulatory disorders].

The disturbances of the primary acral blood supply show fluent transitions from the angiopathic position of the reaction over the digitus mortuus to Raynaud's disease. The functional vascular disturbances at stage II of Raynaud's disease change even to an organic arterial process. The various clinical pictures of the angioneuropathies can be differentiated in every ambulatory surgery in the majority of cases with the help of an aimed anamnesis on the basis of the clinical findings and by simple functional tests. When there are facts unclear, various apparative methods of diagnostics or the acral arteriography must be used.

Cyanosis↗
PubMed1983

[Conservative therapy of acral circulatory disorders and cutaneous microangiopathies].

Despite the different pathological value of disturbances of the acral blood supply and cutaneous microangiopathies there is a need of treatment of these clinical pictures. It can extend from a simple therapeutic consultation of the patient up to the complex use of adequate rules of life, of physiotherapeutic and pharmacological measures. Under this angle of view the treatment of the angiopathic reactive position, of the digitus mortuus and Raynaud's disease is demonstrated. Of particular interest is the use of new antiviscositants in organic disturbances of the acral blood supply. The treatment of the microangiopathies should also be derived from a complex way of consideration in the sense of therapy step programmes. Taking into consideration the severity of the clinical picture the therapy of acrocyanosis, erythrocyanosis drurum puellarum, cutis marmorata, erythromelalgy, livedo racemosa, perniones, hypertensive and diabetic microangiopathy is discussed. In the secondary forms of the disturbances of the acral blood supply the treatment of the basic disease is in the foreground. The accompanying disturbances of the blood circulation may on principle be treated by means of the same measures, which are promising in the primary clinical pictures.

Balneology↗
PubMed1983

Species-dependent variables in blood cerebrospinal fluid barrier for proteins.

Cerebrospinal fluid/plasma concentration quotients for immunoglobulin G (I) and albumin (A) were determined in guinea pigs. Age and sex-dependent influences on these quotients (I/A) were analyzed and compared with the values for human lumbar, cisternal and ventricular cerebrospinal fluid. The ratio I/A, which characterizes the molecular size-dependent selectivity of the blood cerebrospinal fluid barrier function for proteins, was found to be a constant in a single species, but showed marked interspecies differences.

Aging↗
PubMed1982

[Epidemiology of hyperuricemia and gout].

Also in the GDR during the last 10 years the frequency of the disturbances of the purine metabolism increased. With a rate of prevalence of about 20% this anomaly of metabolism reaches the highest percentage of the metabolic disturbances. The increasing accumulation of the disturbances of the purine metabolism on account of complex examinations depends above all on the changed eating and drinking habits of the population. Also influences on body-weight, blood pressure and bodily activities are to be taken into consideration. Sex, age and genetic loads belong to the non-correctable factors of influence. The influences of methodical problems must particularly be taken into consideration in long-term studies. The accumulation of uric arthritis is still relatively small in the GDR in contrast to other industrial countries. However, in our county exact epidemiologic investigations are still necessary.

Body Weight↗
PubMed1982

[Extraarticular diseases and findings in gout].

Disturbances of the renal function, urolithiasis and hypertension are observed in a high percentage already in the asymptomatic stage of the primary hyperuricaemia. They determine above all the fate of the patient with gout. In primary uric arthritis over-accidentally frequently obesity, steatosis hepatis, hyperlipoproteinaemias, manifest diabetes mellitus and premature arteriosclerosis are found, without up to now proving a causal connection. Also these diseases often coin the clinical picture and demand particular attention in the diagnostics of patients with gout.

Arteriosclerosis↗
PubMed1982

[Prevention and therapy of disorders of purine metabolism].

To the most important prophylactic measures for the prevention of a disturbance of the purine metabolism belong the normalisation of the body-weight, a mixed diet without protein carriers containing much purine, a regular physical activity, a life without hectic conditions as well as without an excess of conflict situations and a moderate taking of alcohol. These recommendations are of particular importance for patients endangered by gout. In patients with hyperuricaemia they form the basic treatment for the prevention of a uric arthritis, a urate nephrolithiasis and a gouty nephropathy. The clinical and paraclinical results of the directives must be continuously tested with regard to the effectiveness. To the comprehensive prophylaxis also belong the preventive measures against an associated disturbance of an associated disturbance of metabolism or concomitant disease. An additional medicamentous therapy is necessary, when the success of the basic therapy is insufficient and there are already organ manifestations of a disturbance of the purine metabolism. The application of antihyperuraemic drugs corresponding to types should be performed by uricostatic drugs, uricosuric drugs and citric acid-citrate mixtures. The approach according to a 3-step-programme is most useful. In the treatment of the concomitant diseases certain preventive measures are to be taken into consideration. The therapy of an acute attack of gout is at present without any essential problems. Patients with disturbances of the purine metabolism need a permanent care by the physician.

Arteriosclerosis↗
PubMed1979

[Modern differential-therapeutic aspects of the drug therapy of chronic gout].

The present paper adopts a definite attitude to the differentiated therapy of the disturbances of the uric acid metabolism. This demands an exacter subdivision of the kind of the metabolic disturbance (types Ia, Ib, IIa, IIb, and III). On the basis of this classification in types an individually adapted therapy is possible. It might form the prerequisite of a still more effective meeting of the nephrogenic complications of the gout and of the reduction of the side effects of the necessary permanent therapy.

Allopurinol↗