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Biomedical subjects

P U Koller

Publications and source records attributed to P U Koller.

At least 19 recordsLinked to original sources
PubMed1989

Alpha-amylase determination with the Reflotron reagent carrier system: use of whole blood, plasma, and serum, and effect of isoenzymes.

In the Reflotron Amylase dry-reagent carrier system (Boehringer Mannheim GmbH) a new substrate is used for determining total amylase (EC 3.2.1.1) activity:indolyl-alpha-D-maltoheptaoside. The procedure shows low imprecision (median CV less than 3.2%), and results for sera, plasma, and capillary and venous blood (y) correlate well with those of a conventional alpha-amylase method involving p-nitrophenyl (PNP)-maltoheptaoside substrate (x) (for 209 blood samples: y = 0.981x + 9.7; r = 0.994). Correlation was also excellent with a method involving maltotetraose as substrate (r = 0.987). Attachment of an indoxyl residue rather than a PNP group to the maltoheptaoside did not affect the substrate response to pancreatic or salivary isoenzyme activity. Therefore, the relative proportion of these isoenzymes did not affect the correlation between the Reflotron Amylase reagent carrier and the alpha-amylase PNP-maltoheptaoside method. With a reaction time of less than 3 min, this system is especially suitable for amylase determination in situations where a prompt result is required.

Humans↗
PubMed1988

A multicentre study of the new Reflotron system for the measurement of urea, glucose, triacylglycerols, cholesterol, gamma-glutamyltransferase and haemoglobin.

Reflotron, a solid phase reagent technology capable of measuring a wide range of analytes on whole blood, plasma or serum samples has been evaluated with reference to urea, glucose, cholesterol, triacylglycerols, haemoglobin and gamma-glutamyltransferase analyses. The results show good concordance with conventional wet chemistry methods, with comparable imprecision. Performance is not affected by variations in haematocrit up to 0.55 or bilirubin concentrations at least up to 250 mumol/l.

Blood Glucose↗
PubMed1982

[Clinical importance of sampling method in leucocyturia (author's transl)].

The clinical importance of the method of obtaining a urine sample (spontaneous vs. catheter and midstream specimens) was investigated in 507 in-patients. Whereas leucocyturia was demonstrated with the Cytur-Test on spontaneous urine from 105 patients, only 30 out of 89 of these patients showed leucocyturia in catheter or midstream specimens one to three days later. All 30 positive cases were found to have a disease state which causes leucocyturia. A direct comparison between spontaneous urine and midstream or catheter urine taken at the same time in 68 patients showed a leucocyturia in 20 cases in spontaneous urine which could not be confirmed in the midstream or catheter urine. This drastic difference shows the importance of the method of obtaining specimens in estimating leucocyturia.

Diagnostic Errors↗
PubMed1982

[Rationalization of urine analysis while maintaining diagnostic accuracy (author's transl)].

Screening 720 morning urinary samples for WBC, RBC and protein by test-strip, the number of subsequent microscopic examinations of urinary sediment was reduced to about half, without missing any significant number of clinically significant findings (4.4%). On the other hand, a large number (21.3%) of cases with obviously false-negative sediment findings were revealed. These were largely due to lysis of WBC and RBC, as well as poorly standardized methods of examining urinary sediment. But they could also have been due to differences in subjective criteria employed by the technicians. Our results indicate that using test-strips for screening, clinical routine examination can be rationalized, taking about half the time needed for sediment examination, with more potentially significant findings being discovered than missed.

Clinical Laboratory Techniques↗
PubMed1982

[Diabur-Test 5000 - a new test strip for urinary sugar control in diabetic patients (author's transl)].

Diabur-test 5000, a new test strip for estimation of urinary glucose, was compared with the hexokinase glucose-6-phosphate dehydrogenase method in more than 2500 urine samples. By combination of two test ranges glucose concentrations of up to 5% can be detected by the strip test. After a reading time of 2 minutes, very precise estimation of urinary glucose is possible in eight steps from negative to 5%. False estimations of more than one color step virtually do not occur. Ketone bodies, salicylic acid and several antibiotics do not influence the test strip. Ascorbic acid shows a slight influence only in concentrations above 40 mg/dl. This influence disappears with glucose concentrations of more than 0.5%. Good correlation with the reference method, wide range of readings and simple handling make the test strip suited for the laboratory and particularly for self control of diabetic patients.

Anti-Bacterial Agents↗
PubMed1981

[Reference values for a new alpha-amylase determination in serum and urine (author's transl)].

Reference values for a new alpha-amylase estimation using maltoheptaose as substrate were determined in 251 healthy men and women in serum at 25 degrees C and at 30 degrees C and in urine at 25 degrees C. Sex dependency was not demonstrable. The following reference ranges are suggested: 30-100 U/l in serum at 25 degrees C, 40-130 U/l in serum at 30 degrees C, and 500 U/l in urine at 25 degrees C. In addition reference values for the relative percentual amylase-creatinine clearance (25 degrees C) of 0.25-4% and for amylase activity per mg creatinine (urine 25 degrees C) of 0.02-0.25 U/mg creatinine are suggested.

Adolescent↗
PubMed1981

[Cytur-test used in nephrology out-patients (author's transl)].

Assessment of the value of test strips for demonstration of white cells in urine (Cytur-test) in 161 out-patient urine samples showed that a quick method such as the Cytur-test is no substitute for conventional methods. In hospital nephrology out-patients methods such as the Addis count or the investigation of urine sediment cannot be replaced. However, in a preliminary test with test strips information can be obtained as to the white cell content of the urine. Samples from patients in whom Addis count or sediment results showed no pathological leucocyturia, also had negative results in the test strip in a high percentage (84 out of 88 cases). A positive Cytur-test indicates lysed white cells in such cases (4 out of 88 cases). Due to the different sensitivity, as a consequence of a wider negative range, the Cytur-test resulted in fewer positive findings when compared to the other methods: 6 out of 24 in the Addis count and 2 out of 11 in urinary sediment.

Ambulatory Care↗
PubMed1981

[Pancreatic lipase: reference values at 25 degrees C (author's transl)].

Development of a new specific test for estimation of pancreatic lipase activity (EC 3.1.1.3.) required establishment of reference values. An upper reference range of 170 to 190 U/l at 25 degrees C was determined from a group of 368 females and 435 males aged 20 to 70 years. Values below 170 U/l are very likely to be normal, values above 190 U/l are considered pathological. Values between 170 and 190 U/l require further controls. For practical reasons age and sex dependency need not be accounted for as these influences are negligible.

Age Factors↗
PubMed1980

[Determination of urea in blood and serum by the reflotest-urea method (author's transl)].

Tests in five laboratories on pooled sera in three concentration ranges demonstrated good reproducibility of urea concentration with the Reflotest-Urea, at a variation coefficient from 2.4% to 5.0%. Comparison with the urease/GLDH, Berthelot and diacetylmonoxim tests on sera from routine samples indicated good agreement of results even for pathological samples. Using whole blood there was also good agreement with results with plasma of the same samples. The Reflotest-Urea method is simple because different dose ranges can be used and the reaction time of ten minutes is not crucial. In-vitro studies using 37 of the most commonly used drugs in different areas of medicine failed to reveal any interference with the test.

Analysis of Variance↗
PubMed1979

[Creatine kinase: reference values and clinical aspects of the revised standard method (author's transl)].

Reference values for the revised standard method for determining N-acetylcysteine (NAC) activated creatine kinase (CK) activity in serum are presented, based on samples from 432 out-patients and 423 in-patients of either sex. CK activity was not age-related. The suggested range of normal values for serum CK activity, measured by the revised standard method at + 25 degree C, is 10--70U/l for females and 10--80U/l for males. Where an elevation of serum CK activity is of uncertain cause, the CK/GOT ratio had been recommended, additional to the CK-MB isoenzyme, for distinguishing between damage to heart and skeletal muscle. The significance of this ratio was checked with the revised standard CK method and the optimized GOT determination. The point of separation between myocardial and skeletal muscle damage is 10 by this methods. In order to use this ratio, total CK activity must be over 150 U/l and liver disease or secondary liver involvement must have been excluded. The CK/GOT ratio gains in importance through findings which limit the specificity of CK-MB as a purely myocardial (infarct)-specific isoenzyme.

Adolescent↗
PubMed1978

[Results of using the reflomat for determining glucose concentration, and the influence of glycolysis inhibitors on Reflotest-glucose (author's transl)].

There was a close correlation between the hexokinase-glucose-6-phosphate-dehydrogenase method and reflomat/Reflotest-glucose on capillary blood samples without addition of glycolysis-inhibitors. The relative deviations were less than 10% over the entire range. In systematic studies set up to determine the influence of sodium fluoride and sodium monoiodo-acetate on the reflomat/Reflotest glucose system it was demonstrated that sodium monoiodo-acetate can be used when determining glucose with reflomat/Reflotest glucose, while sodium fluoride produced false values with this system.

Blood Glucose↗