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P V Dmitriev

Publications and source records attributed to P V Dmitriev.

4 recordsLinked to original sources
PubMed2003

Yeast telosome complex: components and their functions.

Telomeres are multi-component DNA-protein complexes protecting linear chromosome termini against degradation and fusion. Telomeres and their components are involved in many essential processes: control of cell division number, regulation of transcription, reparation. They are necessary for correct chromosome segregation during cell division. Telomere protein content and DNA length are under control of different factors, such as components of replication and reparation machinery and cell division cycle kinases. Here we discuss the participation of components of yeast telomere (telosome complex) in different cellular processes, telomeric DNA length regulation pathways, and some issues of telosome evolution.

DNA, Fungal↗
PubMed2001

A specific DNA-dependent DNA polymerase is associated with Saccharomyces cerevisiae telomerase.

The telomere DNA of most eucaryotes consists of tandem DNA repeats and a number of associated proteins. The synthesis of the G-rich DNA strand is performed by the telomerase complex. The complementary C-strand is synthesized by DNA-dependent DNA polymerases. Using telomerase reverse transcriptase tagging followed by immunoprecipitation coupled with two-step purification, we have found a specific DNA-dependent DNA polymerase activity associated with telomerase of Saccharomyces cerevisiae. Investigation of the biochemical properties of this DNA polymerase activity confirms the hypothesis of tight interaction of DNA polymerase delta with telomerase.

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