PubMed Health⌕ Search

Biomedical subjects

P Vago

Publications and source records attributed to P Vago.

14 recordsLinked to original sources
PubMed1991

Effects of castration, Depo-testosterone and cyproterone acetate on lymphocyte T subsets in mouse thymus and spleen.

The effects of testosterone on the relative proportion of Thy 1.2, CD4 (L3T4) and CD8 (Lyt-2) cells in thymus and spleen were studied after castration and administration of Depo-testosterone (DT) separately or together with cyproterone acetate (CA) (an antiandrogen) in BDF1 mice. Injection of 0.5 mg/100 g body weight of DT during 2 weeks decreased significantly the number and proportion of double positive (DP) (CD4+ CD8+) and increased the percentage of single positive (SP) CD4+ (CD4+ CD8-), whereas there was a slight decrease in the Thy 1.2+ cells in the thymus. In parallel, we observed an increase in CD8+ (CD4- CD8+) cells in the spleen. The androgen deprivation after 3 weeks of castration induced a decrease in the percentage of CD4+ cells in thymus and both CD4+ and CD8+ cells in spleen. Injection of CA (0.5 mg/100 g body weight) had the same qualitative effects as DT on the proportion of lymphocyte T subsets in castrated mice. However, the combined activities of DT and CA were greater than either alone. These data indicate the main role of testosterone in the distribution of CD4+ and CD8+ cells in male mice. The similar effects of CA and DT in the lymphoid organs may suggest a difference between androgen receptors of sexual and lymphoid organs.

Androgen Antagonists↗
PubMed1991

[Cytometric profile of the DNA content in pathologies of the uterine cervix].

Through flow cytometry, we have analysed DNA content of cervical cells recovered by scrapping the uterine cervix in 1) 103 women without human papillomavirus (HPV) infection and cervical intraepithelial neoplasia (CIN), 2) 42 patients with HPV infection without CIN, and 45 patients with CIN. We have observed four different DNA patterns: 1) normal DNA pattern, 2) increased and heterogeneous DNA pattern (IH), 3) increased S G2 + M phases, and 4) DNA aneuploid pattern. Statistical calculation has emphasized the significant correlation linking flow cytometric DNA pattern with cytologic diagnosis. HPV infection without CIN was associated with IH DNA pattern and CIN with increased S G2 + M phases or DNA aneuploid pattern. These results point out the interest of flow cytometric analysis of DNA content in uterine cervix pathology and in more extend in infectious or preneoplastic pathology.

Carcinoma in Situ↗
PubMed1991

Nycthemeral variations on LPS- and BCG-induced PMN migration in normal mice.

This study was performed to determine whether temporal variations could exist in polymorphonuclear neutrophil (PMN) migration induced by LPS or BCG in mice. LPS- and BCG-impregnated cell traps were implanted at 6 different times of the day and removed after 480 min. The PMN number per square unit (mm2) was counted and the results were expressed as means +/- S.E. in 8-10 mice. The results showed that nycthemeral variations occurred in both LPS- and BCG-induced PMN migration. Between 5.00 and 9.00 h a maximum value of 4210 +/- 270 and 1920 +/- 486 PMN/mm2 were obtained with BCG and LPS respectively; at 17.00 h a minimum value of 1300 +/- 270 with BCG and 396 +/- 127 PMN/mm2 with LPS were observed. There was no significant nycthemeral variation in saline-induced PMN migration.

Animals↗
PubMed1990

[Cytometric profiles of DNA content in cervical intraepithelial neoplasia (CIN)].

The cervical cytologic screening is responsible for the overall decline in the incidence of cervical cancer. Despite this apparent success, the cytologic assessment by light microscopy of cervical smear has recently been shown to have deficiencies particularly in cervical intraepithelial neoplasia (CIN). Now, this pathology of the uterine cervix is increasingly becoming recognized as a major health problem in young women because of its high frequency and its association with the development of cervical carcinoma. Flow cytometry (FCM) is widely used as a rapid method to analyse DNA content of a great number of cells on a cell by cell basis. The authors have applied FCM to CIN in order to reinforce the cytologic screening of cervical smears. Three abnormal DNA patterns have been observed: increased and heterogeneous DNA pattern; increased S and G2+M phases; abnormal DNA content. The statistical calculation has emphasized the significant relation linking these DNA patterns with the cytologic diagnosis and the severity of CIN. Therefore, FCM is a powerful complementary tool in cervical pathology.

Carcinoma in Situ↗
PubMed1990

[Qualitative approach of DNA content analysis by flow cytometry].

One step DNA staining procedures are very easy to use. Reduced staining time and small amount of needed components explain the world wide use of these protocols. The heterogeneity of the results obtained with these technics during a study of different mice organs has let the authors to think that there was a close relationship between this heterogeneity and DNA staining technics. The authors have observed the same discordances with some human tissues (parathyroid, prostatic adenoma...). They disappear when the same cells are stained with a procedure that associates to propidium iodide and RNAse a pretreatment of nuclei by trypsin and spermin. These molecules are DNA stabilizing components. So one can develop the hypothesis that the abnormalities observed with the one step procedures are due to a decondensated state of DNA that allows the fixation of a higher amount of stain. The main point will be to find a direct relationship with different physiological or pathological states. Now, it is necessary to sensibilise users against the risk of false abnormal DNA content detection through the one step procedures.

Animals↗
PubMed1989

[Flow cytometry. Principles and methods for cell studies].

Through flow cytometry cells are investigated by optical, electrical and electronic components. On a rate of several hundred cells per second, quantitative multiparametric cells studies are realized on a cell by cell basis. Cells in monodisperse suspension are drawn by a stream of fluid which aligns them before their interception by a beam of light, generally produced by Laser. The generated optical signals (diffused lights and emitted fluorescences), related to biological properties of the cells, are conveyed to photomultipliers which transform light energy into electric currents. The signals are quantified by electronic and processing components which can drive cell sorting. Flow cytometry is playing an increasingly significant role in the practice of the clinical laboratory in several areas i.e. oncology (DNA content measurement) or immunology (lymphocytes phenotyping).

Electronic Data Processing↗
PubMed1988

[Inter-organ differences of the cytometric DNA content in mice: relation of the staining method].

With one step DNA staining methods including cell membrane lysis and RNase treatment, we regularly observed a higher fluorescence emission in liver nuclei compared to bone marrow nuclei in C57BL/6 mice. Therefore this study was conducted in order to emphasize such a phenomenon in other organs and to assess if higher fluorescence emission was related to higher DNA content or staining procedure failure. Liver, bone marrow and testis were removed from Swiss, BDF and C57BL/6 mice. The following samples were prepared: 1) liver cells with TRBC (TRBC = Trout Red Blood Cells = internal standards), 2) bone marrow cells with TRBC, 3) testis cells with TRBC and 4) mixtures of liver, bone marrow and testis cells. The staining procedures were: A) one step pH 10 procedure described by Vindelov (Virchows Arch. B. Cell Path., 1977, 24, 227-242), B) same procedure with twice RNase concentration, C) first method with twice NP 40 concentration and D) three steps procedure including Trypsin and Spermine treatment (Vindelov et al., Cytometry, 1983, 3, 323-327). In protocols A, B and C, "Diploid cells/TRBC" ratio differed significantly between liver, bone marrow and testis nuclei. Moreover, 3 distinct populations of diploid cells were present in samples 4. In protocol D, "Diploid cells/TRBC" ratio were identical between liver, bone marrow and testis nuclei. In samples 4, only 1 population of diploid cells has been observed. This study shows that DNA stabilization by polyamine and protein degradation by protease could act on Propidium Iodide fixation and/or fluorescence emission, with significant differences according to the origin of the cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1987

Effect of aerobic training quantity on the VO2 max of circumpubertal swimmers.

Maximal oxygen uptake (VO2 max) was measured in 38 swimmers aged 10-14 years. Thirty of 38 boys participated in this study for at least 2 consecutive years. Group 1 consisted of 23 subjects (48 measures) who trained for 7 h/week while group 2 consisted of 15 subjects (27 measures) who trained for 14 h/week. In group 2, VO2 max normalized to body weight was significantly higher at 14 years of age than at 10, whereas the increase was nonsignificant during this period in group 1. The subjects of group 2 showed a large increase of VO2 max/kg body weight from the age of 13, which corresponded in this study to the age of peak height growth velocity. The differences between the two groups were statistically significant at both 13 (P less than 0.02) and 14 years of age (P less than 0.05). At 13 and 14, the most trained subjects also showed significantly higher (P less than 0.05) values of maximal oxygen pulse/kg body weight (VO2 max/kg/HR max). Maximal heart rate (HR max) was similar in the two groups between 10 and 14 years of age. Therefore, we conclude that an increase in a training program of the aerobic type induces a large increase in VO2 max from the age of peak height growth velocity. This is likely due to an increase in the stroke volume.

Adolescent↗
PubMed1987

Is ventilatory anaerobic threshold a good index of endurance capacity?

This study was undertaken to assess whether ventilatory anaerobic threshold (T vent) reflected endurance capacity (EC) in sports medical control. Fifteen subjects performed two cycle ergometer tests. The first was a maximal exercise test, which consisted of increasing the load 20 W/min until exhaustion. During this test, the gas exchange anaerobic threshold was determined and VO2 max was measured. The second was an endurance exercise test, which consisted of asking the subject to work, as long as possible, a load representing 80% of his maximal aerobic power. During this test, we measured endurance time (ET). The statistical analysis showed the lack of relationship between VO2 max and ET and the linear correlations between VO2 T vent ml/min/kg and ET min (r = 0.521, P less than 0.05), VO2 T vent l/min and ET min (r = 0.524, P less than 0.05), and T vent % VO2 max and ET (r = 0.738, P less than 0.01). These results establish that ventilatory anaerobic threshold actually reflects endurance capacity. This relation can be explained referring to the muscle energetic metabolism during exercise. Therefore, T vent should be determined systematically in addition to VO2 max during maximal exercise tests to better evaluate physical fitness.

Adolescent↗
PubMed1987

[Cytometric analysis of the DNA content of the thymus, spleen and liver in C57BL/6 mice].

Flow cytometric analysis of cell DNA content in the mouse C 57 BL/6 shows better results with fine needle aspiration than with mechanical dissociation of the tissues. There is no difference fresh and deep frozen stored tissues. In the liver, it has been found two cell populations with different DNA content including diploid and tetraploid cell lines. In the spleen and the thymus, only diploid cells are encountered with different cell cycles belonging to the organ.

Animals↗
PubMed1987

[Cytometric study of the effects of destruxin E on leukemic cells in mice].

The activity of destruxin E, a cryptogamic toxin isolated from the hyphomycete Metarhizium anisopliae, was studied on mouse leukemia cells (L 1210 and P 388) in culture. Besides a cytotoxic effect, a cytostatic effect (increase of diploïd cells proportion) was observed with all doses for P 388 (from 10 micrograms/ml to 0.001 microgram/ml) and to a concentration of 0.1 microgram/ml for L 1210. At the lower doses, the effect on cellular DNA content appears distinctly.

Animals↗
PubMed1987

[The chronobiology of inflammation].

The inflammatory reaction is characterized by a multifrequency time structure with prominent circadian, ultradian and infradian rhythms in cell proliferation and cell function. The circulating blood cells show highly reproducible circadian rhythms in human and in animals. Daily fluctuations of concentrations of haematologic chemical and endocrinological parameters are described. Circannual variations of several hematologic parameters are presented. Chronobiological studies of plasma exudation in carrageenan paw oedema show a circadian and a circannual rhythm. Daily and annual changes in migration of polymorphonuclear leucocyte produced by BCG are studied in mice. Differences in response are observed in adrenalectomized and castrated animals. The susceptibility of cells and tissues at different stages of their circadian or circannual rhythms lead to the development of a chronotherapy of inflammation.

Animals↗
PubMed1986

[Optimization of the method of progressive hypercapnia by determination of the sensitivity threshold].

Ventilatory response to CO2 rebreathing is a method which allows to evaluate the reactivity of chemoreceptors. However this method doesn't study the sensibility threshold, i.e. the Pe.t.CO2 value for which the ventilatory response appears clearly. This sensibility threshold was measured in 10 healthy subjects by rebreathing a gas mixture: 7% CO2 and 50% O2 to avoid hypoxy. It was defined as the value of Pe.t.CO2 for which the ventilation was above the tidal ventilation + 2 standard deviations. The sensibility threshold (51 +/- 4.35 mm Hg) was independent of the reactivity slope represented by the slope of the linear relation between minute ventilation (VE) and Pe.t.CO2 (1.34 +/- 0.60 l/min/mm Hg/m2) and consequently appears as an interesting parameter in order to evaluate the ventilatory response to CO2 by rebreathing.

Adult↗