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Biomedical subjects

P Valouch

Publications and source records attributed to P Valouch.

12 recordsLinked to original sources
PubMed1993

On vertebral body growth.

We have studied development of vertebral bodies with special regard to the area of contact of the vertebral body with the intervertebral disc. We have investigated complete serial sections of fetuses of the 2nd to 3rd month of intrauterine life, microscopic preparations from children, adolescents and young adults. The results of our studies and deductions from our findings are as follows: 1. Cartilaginous plates on contact surfaces of vertebral bodies with intervertebral discs are genetically parts of vertebral bodies, not of the discs. 2. During ontogenesis and growth period of life typical growth (epiphyseal) cartilages with all layers described on growth cartilages of long bones are formed on the surface of these cartilaginous plates facing the vertebral bodies. 3. From the point of view of its growth, the vertebral body should be considered to be a long bone. 4. Unlike other long bones of the skeleton, vertebral body epiphyses never ossify, and after the end of the growth period of life they are reduced into thin plates of hyaline cartilage which are situated between vertebral body and intervertebral disc. 5. We assume nucleus pulposus is not a persistent residuum of notochord. 6. According to our findings the link of sacral vertebral segments is from the very beginning of development typical synchondrosis with bipolar physes without formation of primordium of intervertebral disc.

Adolescent↗
PubMed1990

[Levels of large proteoglycan aggregate binding proteins and the level of small proteoglycans in healthy human articular cartilage and in osteoarthrosis].

The investigation was focused on the electronmicroscopic localization of one structure of the aggregate of the large proteoglycan--binding proteins and the small dermatan sulphate proteoglycan, using specific antibodies prepared against these structures and the complex colloid gold--protein A. The authors investigated the incidence and distribution of these structures in the intercellular area of ultrathin sections of the sound and arthritic human articular cartilage. In the arthritic cartilage, consistent with biochemical findings, the authors detected a loss of the binding protein or its disappearance and conversely an increase of the small dermatan sulphate proteoglycan.

Carrier Proteins↗
PubMed1989

[Degenerative changes in intervertebral disks].

A histotopographic study of lumbar spine was made in 27 deceased of the age of 2 to 85 years lacking of any spinal clinical symptomatology. Regressive changes were found in all the intervertebral disks except in infants. A central necrosis was present in 26 cases and pericentral striped necrosis in 19 cases. Other disks showed consequences of necrosis--vascularization, scarring, regenerative proliferation of chondrocytes, ganglion type cavities. A concept of disk lesion as a trigger of further spinal lesions was supported by frequent regressive changes of the disks. Schmorl's (internal) nodes were found in 35 cases, quite often in young persons without clinical symptomatology. The amount of glycosaminoglycans during the disk regression was detected by Safranin O-Fast Green stain with ambiguous results. A hypothetical spontaneous vanishing of the nucleus pulposus in accordance with the age was a topic of discussion. Protrusions of the disks into the spinal canal were not found. Initial osteophytes at ventral margins of vertebral bodies were identified in 49 cases, at dorsal margins in only 3 cases.

Adolescent↗
PubMed1985

Ultrastructural localization of rRNA in HeLa cells, rat liver cells and Xenopus laevis oocytes by means of the monoclonal antibody--protein a--gold technique.

The postembedding localization of rRNA was investigated in ultrathin sections of HeLa cells, rat liver and Xenopus laevis oocytes by means of the monoclonal antibody to rRNA and protein A-gold technique. The incidence of gold particles was highest in nucleoli and cytoplasmic areas containing ribosomes. The chromosomes were labelled less than the surrounding cytoplasm in mitotic HeLa cells. In nucleoli of HeLa cells and rat hepatocytes, the labelling of areas containing ribonucleoprotein components was greater than the labelling of fibrillar centres. In segregated nucleoli of X. laevis oocytes, the labelling of the granular region substantially exceeded that of the fibrillar regions. The incidence of nucleoplasmic gold particles in interphasic HeLa cells was found to be slightly increased in the vicinity of nucleoli. The labelling of clusters of interchromatin granules in rat hepatocytes was not significantly different from that of the rest of the nucleophasmic interchromatin spaces.

Animals↗
PubMed1985

Enthesopathy of the hip joint.

Morphologically, enthesopathy appears as dystrophic calcification or ossification of tendon insertion. In the study of the initial stages of arthrosis on necroptic material roentgenologically 158 and of these histologically 62 hip joints were investigated. A typical picture of so-called non-inflammatory enthesopathy was present in thirteen cases on the major and in three cases on the minor trochanter. Histologically, we observed enthesopathy-like changes, but to a much lesser extent in the insertions of the articular capsule. These pictures which on the X-ray resembled initial osteophytes were found seven times on the head of the femur and fourteen times on the edge of the joint acetabulum. There was no correlation to osteoarthrosis.

Female↗
PubMed1984

The calcified-noncalcified cartilage interface: the tidemark.

Tidemark is an interface which may better be defined by biochemical methods than by morphology. It originates, by chondrocyte activity, between calcified and noncalcified cartilage layers of any kind, hyaline or fibrous, in areas exposed to either loading (joint) or pulling (insertion). In the articular cartilage it appears with skeletal maturation, in other localizations it is age-independent. It should be regarded as a special instance of a broader phenomenon of the calcification/mineralization front. Inside the joint cartilage its changes reflect the slow remodelling of the calcified layer and its inapparent shift towards the surface of the articular cartilage. In the marginal transitional zone of the joint, tidemark smoothly passes into the periosteum. Chondrocytes on both sides of the tidemark are positive for alkaline phosphatase and the positive reaction continuously goes on to the periosteum.

Adolescent↗