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Biomedical subjects

P Vestergaard

Publications and source records attributed to P Vestergaard.

At least 109 records · Page 6Linked to original sources
PubMed1979

[Recent developments in lithium treatment: is the kidney affection dangerous? (author's transl)].

In our hospital, we are studying the renal function of more than 200 patients under lithium treatment. The glomerular function seems to be affected to a small extent, even by long-term lithium administration, and there have been no instances of progressive deterioration of renal function with azotemia. Most of the patients have impaired water reabsorption with polyuria and polydipsia. This is frequently combined with lowered concentrating ability. Polyuria and lowered concentrating ability are not in themselves life-threatening. However, development of dehydration may lead to lithium poisoning, and patients under lithium treatment should be advised to drink extra fluid under circumstances when the fluid balance may become negative. Further studies are required for definitive recommendations concerning extra monitoring of kidney function and lowering of lithium doses.

Bipolar Disorder↗
PubMed1978

Mass spectrometric determination of cocaine and its biologically active metabolite, norcocaine, in human urine.

A gas chromatographic mass spectrometric assay has been developed for the determination of cocaine and its pharmacologically active metabolite, norcocaine, in human urine. [2H3]Cocaine and [2H3]norcocaine were used as internal standards. The assay utilizes selective focusing to monitor in a gas chromatographic effluent the molecular ions of cocaine, [2H3]cocaine and the fragment ions of trifluoroacetylated norcocaine, [2H3]norcocaine generated by electron impact ionization. The assay can measure 2 ng ml-1 each of cocaine and norcocaine with about 5% precision. The curves, relating the amounts of cocaine and norcocaine added to control urine per 'fixed' amounts of their labeled analogs, versus the appropriate ion intensity ratios are straight lines with nearly zero intercepts and slopes of 0.98 +/- 0.01 and 0.98 +/- 0.02, respectively. The methodology is used for the analysis of urinary cocaine and norcocaine from three human subjects who received 100 mg cocaine-HCL intravenously.

Cocaine↗
PubMed1978

Quantitation of cocaine and its principal metabolite, benzoylecgonine, by GLC-mass spectrometry using stable isotope labeled analogs as internal standards.

A quantitative GLC-mass spectrometric assay was developed for the determination of cocaine and its principal metabolite, benzoylecgonine, in human urine. The assay utilizes selective ion focusing to monitor in a GLC effluent the molecular ions of cocaine and benzoylecgonine generated by electron-impact ionization. Cocaine-d(3) and benzoylecgonine-d(3) were the internal standards. The assay can measure 2 ng of cocaine/ml and 5 ng of benzoylecgonine/ml with about 5% precision. The curves relating the amounts of cocaine and benzoylecgonine added versus the amounts found over a large range of cocaine and benzoylecgonine concentrations were straight lines with nearly zero intercepts and slopes of 0.98 +/- 0.01 and 0.97 +/- 0.01, respectively. The method was used for the analysis of urinary cocaine and benzoylecgonine in cocaine addicts. Assay specificity was confirmed by complete identity of the mass spectra of cocaine and benzoylecgonine with those of authentic materials.

Chromatography, Gas↗
PubMed1978

Mass fragmentographic determination of methadyl acetate in urine using stable isotope labeled analog as internal standard.

A quantitative GLC-mass spectrometric assay was developed for the determination of methadyl acetate in urine. The assay utilized selective ion focusing to monitor, in a GLC effluent, the M--15 ion generated by electron-impact ionization of methadyl acetate. Methadyl acetate-d4 was used as an internal standard. The assay can measure 10 ng of drug/ml with about 6% precision. The curve relating the amounts of drug added to control urine versus the amounts experimentally found over a large concentration range is a straight line with a slope of 0.98 +/- 0.02 and a nearly zero intercept. Assay specificity was confirmed by complete identity of the mass spectrum of methadyl acetate in the biological extract with that of the authentic material. The method was used for the urinary analysis of methadyl acetate in a rabbit given a single intravenous dose. The animal excreted less than 1% of the intact drug with a half-life of approximately 15 hr. Consequently, the long-acting characteristic of methadyl acetate must be attributed to its metabolism into active metabolites.

Animals↗
PubMed1978

Oesophageal disease revealed by endoscopy in 1,000 patients referred primarily for gastroscopy.

Of 1000 patients referred primarily for gastroscopy, almost 18% had endoscopic signs of clinically important oesophageal disease. Erosive oesophagitis, a disease that is notoriously difficult to diagnose on X-ray, was demonstrated in alsmot 10% and oesophageal varices in 3.6% of the patients. It is concluded that a thorough examination of the oesophagus should be included in every routine upper gastrointestinal endoscopy.

Diverticulum, Esophageal↗
PubMed1978

Comparison of the use of inorganic and organic bases in the Zimmermann reaction for 17-oxosteroids.

A comparison has been made between inorganic and organic bases proposed for use in the Zimmermann reaction for 17-oxosteroids. The use of inorganic base in pyridine solution is preferred for large scale liquid chromatography because of its high sensitivity and its better wetting characteristics. Several of the organic bases tested have desirable characteristics when used for total group assays and may be preferred by some for such assays.

17-Ketosteroids↗
PubMed1978

The specificity of multi-column liquid chromatography of neutral urinary steroids as evaluated by comparison with parallel gas chromatography and mass spectrometry.

The use of gas chromatography/mass spectrometry is advocated as the best currently available methodology for the validation of a routine assay for steroid analysis. This is documented by comparative studies by gas chromatography/mass spectrometry and spectrophotometry of peak material obtained in routine assays by multi-column liquid chromatographic techniques.

Chromatography, Gas↗
PubMed1978

Quality control measures in the assay of common urinary steroids as applied to a multi-column chromatographic procedure.

Quality control measures in the assay of common urinary steroids include stringent controls not only with details of the procedure but also, through a steady monitoring of the output, with the use of "blind" duplicates, the application of estimates of duplicate variability, comparison of the sum of individual steroids chromatographically estimated with independent total group assays and the use of internal standards in the procedure.

Chromatography, Liquid↗
PubMed1978

Excretion of common neutral steroids in healthy subjects as estimated by multi-column chromatography.

Excretion data for common neutral urinary steroids from a total of 330 healthy subjects from different parts of the world and of different sex and age are given. The estimations, which have been performed by multi-column liquid chromatography, include 24 h excretion values for both common 17-oxosteroids and the common metabolites of cortisol, including the cortolones and the cortols. Comparisons are made with values from the world literature and with isotope experiments.

17-Ketosteroids↗
PubMed1978

Multi-column chromatography of urinary steriods and adrenocortical dysfunction.

The potential of the multi-column assay for urinary neutral steroids in work with samples from patients with adrenocortical pathology is demonstrated through analyses performed on urine samples from Cushing and congenital adrenal hyperplasia cases, after modification of the routine methodology to include the quantitation of additional steroids of particular importance for pathological samples.

Adrenocortical Hyperfunction↗