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Biomedical subjects

P Yoshihara

Publications and source records attributed to P Yoshihara.

4 recordsLinked to original sources
PubMed1992

Clinical evaluation of oral fluid samples for diagnosis of viral hepatitis.

Oral fluid samples were compared with serum samples as a specimen source for hepatitis A, B, and C virus markers. Oral fluid was obtained with a treated absorbent pad and tested by using existing commercial enzyme immunoassays with only minor modifications. Compared with serum sampling the sensitivity and specificity of oral sampling were 100% (51 of 51 samples) and 98% (46 of 47 samples) for hepatitis A virus immunoglobulin M, 100% (29 of 29 samples) and 100% (29 of 29 samples) for hepatitis B virus surface antigen, and 100% (13 of 13 samples) and 100% (13 of 13 samples) for hepatitis C virus antibody, respectively. The decline of hepatitis A virus immunoglobulin M in oral samples was parallel to, though somewhat more rapid than, that of hepatitis A virus immunoglobulin M in serum samples. It is proposed that oral sampling represents a safer and more convenient procedure for reliable hepatitis virus testing than blood sampling and that it has wide application in patient and outbreak management.

Antibodies, Viral↗
PubMed1987

Biochemical studies on a cell surface determinant involved in T-cell proliferation.

Previous studies have shown that a monoclonal antibody (TH5.2) recognizes a cell-surface determinant which is involved in the proliferative capability of T cells. The work reported here demonstrates that the T-cell-surface antigen recognized by TH5.2 is a glycoprotein of 55,000 to 60,000 molecular weight. The molecule shows a single molecular weight species upon reduction and denaturation, and it contains only a few percent of tunicamycin-sensitive carbohydrate structures. As shown in sequential immunoprecipitation studies, the TH5.2 antigen is on a molecule distinct from the interleukin-2 (Tac) receptor and the T4 molecule. Cell-surface antigenic modulation experiments indicate that the TH5.2 antigen does not comodulate with, and therefore is distinct from, the T3, T4, T8, and Leu-5 T-cell antigens.

Antibodies, Monoclonal↗