Biomedical subjects

P Z Lu

Publications and source records attributed to P Z Lu.

4 recordsLinked to original sources

PubMed2001

[Recognition and quantitative contrast characteristic components for root of Chinese angelica].

A method of multi-component quantitative analysis in root of Chinese Angelica has been developed. The analytical mode was reversed-phase HPLC with photodiode array detector. Based on comparison of UV spectra, a software of UV spectra database to assist recognition has been established. The solvent system, a gradient program of mobile phase with 2-propanol/acetonitrile/water can separate a lot of liposoluble components in root of Chinese Angelica. UV detection wavelength was set at 202 nm and the flow rate was 1 mL/min on a Hypersil ODS column. Twenty-three components in two roots of Chinese Angelica were recognized using characteristic parameters of chromatography and UV spectroscopy. It is the base of quantitative contrast to recognize many characteristic components without standard components in root of Chinese Angelica. For two samples of root of Chinese Angelica both have 23 characteristic components and the concentrations of some components were similar and those of others were different. The method is convenient and reproducible. It can be used for the quality control of the root of Chinese Angelica without the use of standards.

Angelica sinensis↗

PubMed2001

[Study of unified analytical method of compatibleness for traditional Chinese medicines. I. Separation, recognition of characteristic components and fingerprint analysis for single and complex medicines].

It is difficult to separate, identify and quantify multi-component in complex traditional Chinese medicines (TCM). Many analytical methods for TCM have been established, but only a few components can be separated and quantified. For analyzing the multi-component and finding new method of compatibility analysis, a strategy--the multi-mode and multi-column chromatographic system and unified analytical method has been proposed by us. A part of the results from the study of astragalus root(A), Chinese angelica root(Ca.) and their complex prescription (Danggui Buxue Tang, DBT) with the analytical mode of reversed-phase HPLC/photodiode array detector as an example of the method for unified separation and recognition of characteristic components and fingerprint analysis was introduced. This is the first part of the results of compatibleness analysis for TCM.

Angelica sinensis↗

PubMed2001

[Study of unified analytical method of compatibleness for traditional Chinese medicines. II. The quantitation of curve fitting].

To introduce method of compatibleness analysis for traditional Chinese medicines, we select a part of the results from the study of astragalus root(A.), Chinese angelica root(Ca.) and their complex prescription (Danggui Buxue Tang, DBT) with an analytical mode of reversed-phase HPLC-photodiode array detector as an example of the quantitation of curve fitting. A new quantitative method, the concentration variation of components as the changing of single prescription dosage in complex prescriptions is discussed. This is the second part of the method of compatibleness analysis for traditional Chinese medicines.

Angelica sinensis↗

PubMed2001

[Characterization and recognition key components in Astragalus membranaceus].

AIM: To separate, identify and quantify multi-components in complex traditional Chinese medicine (TCM) Astragalus membranaceus by unified analysis for systematic study of its relative contents. METHODS: The analytical method was reverse-phase HPLC/photodiode array detector. Based on the comparison of UV spectra, software of UV database to assist recognition has been established. Key components have been found and recognized in two reference Astragalus membranaceus and three samples from different sources. With the unified analytical project, relative peak heights of key components were recognized and contrasted. RESULTS: The water-soluble components can be separated by solvent system A, a gradient program with mobile phase of methanol-water. The liposoluble components can be separated with the solvent system B, a gradient program of mobile phase of 2-propanol-acetonitrile-water. UV detection wavelength was set at 202 nm and the flow rate was 1 mL.min-1 on a Hypersil ODS column. Twenty-nine components in the four Astragalus membranaceus were recognized using characteristic parameters of chromatography and UV spectroscopy. All of the 29 key components in two reference samples and three samples from different sources were shown in their chromatograms to be the same. The concentrations of some the key components in different samples were similar while those of the others were different. The chromatographic peaks of the reference Astragalus membranaceus and two samples were matched with each other. Many different fingerprints were found and differences were shown on their chromatograms. Not only there were differences among three samples from different source, but also there were differences in the two reference samples. CONCLUSION: To find out the key components and recognize them are the basis of quantitative recognition and are very useful when standards are not available. The method of multi-component quantitative recognition in Astragalus membranaceus was developed. The method is convenient and reproducible. It can be widely used for the quality control of the Astragalus membranaceus.

Astragalus propinquus↗