Biomedical subjects
Publications and source records attributed to P de Graeve.
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Castrated rats submitted to androgenic stimulation present hyperseborrhea with an increase in foamy cells associated with an increase in cutaneous lipids and a change in the number of epidermal cell layers. 13-cis-retinoic acid and aromatic retinoid (Ro 10-9359) were given orally during 17 days (10 mg/kg) to castrated rats which had received a testosterone implant. Cyproterone acetate was chosen as the antiandrogenic drug; it was given at doses of 10 mg/kg i.m. once every other day. As opposed to cyproterone acetate, the two substances studied had no effect on the seminal vesicle and ventral prostate. 13-cis-retinoic acid and retinoic aromatic acid increased the total number of epidermal layers: In the case of 13-cis-retinoic acid, the increase involves especially the nonnucleated cell layers, whereas with retinoid aromatic acid it involves the nucleated cell layer. The number of sebaceous glands is not changed by either products, on the other hand, the number of foamy cells is considerably decreased and the weight of cutaneous lipids is decreased as well.
The ureterovesical junction was studied by dissection and serial sections in 50 post mortem specimens. Three points are considered in this paper: the structure of the terminal ureter, the anatomical arrangement of the ureteral hiatus and the ureteral sheath. Study of the structure of the intramural segment of the ureter demonstrated on one hand, the perfect continuity of the terminal ureter with the trigone and on the other hand, the abundance of the fibroelastic connective fibers which like the muscle fibers run longitudinally. The compliance of the intravesical ureter is dependent on the balance between these two components. The modification of this balance can lead to the creation of a functional obstacle. The anatomical arrangement of the ureteral hiatus is described. The inner muscle layer of the detrusor extends almost to the ureteral orifice, the truly submucosal part of the ureter thus being very small. The constitution of the ureteral orifice and its relations to the ureter account for the different positions of juxtaureteral diverticula and transhiatal herniae of the bladder mucosa. The many descriptions of the ureteral sheath appearing in the literature are reviewed in light of the findings from the present study. The juxtavesical segment of the ureter is surrounded by a fibroconjunctive sheath which fixes the ureter to the bladder wall. The transparietal segment of the ureter is ensheathed in its adventitia, whereas a fibromuscular sheath cannot be truly individualized over this ureteral segment.
The thymus and the spleen of guinea Pigs treated with oestrogen were rich in cells showing a special inclusion, the Foa-Kurloff body cell (F.K.). Thymic, spleen and renal cellular extracts were obtained by tissue crushing and ultra-centrifugation from healthy or oestrogen treated animals. The extracts were added to carcinomatous (HeLa) or to non-carcinomatous cells (MRC5 fibroblasts or foetal human lung cells). The thymic extracts in the non-treated animals and the splenic extracts rich in F.K. body cells induced an inhibition in the growth of carcinomatous cells. These effects were increased with the thymus extracts rich in F.K. bodies. The thymic inclusion of F.K. seems therefore to concentrate tumorous thymic fractions.
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Urinary sediment of 11 patients with amyloidosis and 12 without (with proteinuria or in good health) have been sutdied by different morphological techniques. By light microscopy, an amyloid-related substance was occasionally demonstrated both in patients with amyloidosis and in control subjects. Immunofluorescence (IF) showed substance A (amyloid component) to be present in some cases of amyloidosis and in controls. On electron-microscopy, fibrils with characteristic appearance of amyloid substance were found in some cases of amyloidosis (4 out of 11), but were also found in controls. It therefore seems difficult to establish the diagnosis of amyloidosis by microscopic studies of the urinary sediment.
Grafts and injections of cellular extracts from thymus and spleen of guinea pigs, were carried out in puberal and impuberal female rats. These organs are especially rich in Foa-Kurloff cells after oestrogentherapy. Kidney served as a reference material for the same experimental conditions. Controls received cellular injections and grafts from guinea pig organs without treatment. In all cases, ovarian histological and biometric study of recipient animals shows no alteration of the ovarian cycle. On the other hand, grafts and injections of cellular extracts from organs with high concentration of Foa-Kurloff cells induced hyperactivity of internal theca folliculi and of ovarian interstitial cells, only in puberal female rats.
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The changes of the thymic cytologia under the action of genital hormones were studied in giunea pigs. The conclusion of the experiments was: the oestrogens are the only hormones to induce a fast and complete involution of the thymic tissue with appearance of Foa-Kurloff cells'. The cell seems to have all the necessary characters to be the morphologic substratum of an eventual cytocrine secretion of the thymus.
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