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Biomedical subjects

P van Heerde

Publications and source records attributed to P van Heerde.

At least 55 records · Page 3Linked to original sources
PubMed1986

Determination of transferrin receptors on frozen sections of malignant B-cell lymphomas by immunofluorescence with a monoclonal antibody.

A series of 34 B-cell lymphomas was studied for the presence of transferrin receptors by immunofluorescence on frozen tissue sections with a monoclonal antibody. These lymphomas were classified by light microscopic study and furthermore investigated by immunohistochemical and morphometric methods. There were diffuse centrocytic, diffuse centroblastic, and follicular and diffuse centrocytic/centroblastic lymphomas. Significant differences in log mean nuclear area in these four histologic groups were found, as well as a significant correlation between log nuclear area and transferrin receptors as measured semiquantitatively by fluorescence intensity of the cell membranes. In addition, it was found that there is a strong correlation between the transferrin receptor activity and malignancy grading on histologic and morphometric basis. Therefore, the transferrin receptor determination on frozen sections appears to be a good method of malignancy grading.

Adult↗
PubMed1985

Reproducibility and prognostic value of different non-Hodgkin's lymphoma classifications: study based on the clinicopathologic relations found in the EORTC trial (20751).

In the EORTC trial 20751, six pathologists belonging to five different centers classified tumoral lymph nodes from 406 untreated patients with non-Hodgkin's lymphoma (NHL) into three different NHL classifications. NHL were most easily and reliably subdivided according to the growth pattern (the rate of consensus being 93%). Classification according to growth pattern proved to be of prognostic significance. The rate of consensus of classifying cases in the different NHL classifications ranged from 74% for the Rappaport, through 70% for the Kiel to 67% for the international working formulation. It is concluded that NHL are reliably classified according to their growth pattern, and that this subdivision is of primary prognostic significance.

Humans↗
PubMed1984

Cytodiagnosis of non-Hodgkin's lymphoma. A morphological analysis of 215 biopsy proven cases.

A cytomorphological analysis using a large number of variables was applied to 136 fine needle aspirations and 122 imprints from 258 biopsy proven lymphoid lesions, including 203 non-Hodgkin's lymphomas (NHL), 12 true histiocytic neoplasms and 43 lymphoid hyperplasias. Characteristics of the various cell types are described using the Kiel classification predominantly. Two blindly performed consecutive cytological analyses, indicated as Cyt I and Cyt II respectively, were compared with histology. False-positive conclusions did not occur. False-negative conclusions were present in 3.7% of cases. The intra-observer reproducibility between Cyt I and Cyt II was 93%. The inter-observer reproducibility between the 4 authors was examined in 50 cases; consensus regarding malignancy, exact NHL type and benign cytology was 88%, 70% and 100% respectively. Cytodiagnosis of NHL is a reliable method with a high sensitivity which can add substantial information to tissue diagnosis in troublesome cases. Fine needle aspirations, if adequately performed, had the same cellularity, quality and cellular composition as imprints. Aspiration cytology is very useful for the selection of a representative lymph node for surgical biopsy, for the diagnosis of recurrent NHL, for staging the extent of disease, for the diagnosis of cases in which tissue biopsy is not possible, and as a variable for monitoring treatment.

Adolescent↗
PubMed1984

Lymphoid proliferations in the orbit: malignant or benign?

Clinical, pathological, and immunological analysis of 20 patients with ocular adnexal lymphoid disease has demonstrated several parameters which are useful for distinguishing malignant from benign lesions. Patients in the fourth or fifth decade of life presenting with an acute history of pain, oedema, epiphora, double vision, and ptosis, with a mass localised in the lacrimal gland area, are more likely to have a pseudolymphoma or a chronic inflammatory lesion than a true non-Hodgkin lymphoma (NHL). It is not possible to obtain a definite diagnosis without surgical intervention, because only three out of nine patients with orbital NHL had evidence of a monoclonal B cell population in peripheral blood on admission to the Orbital Centre. Furthermore it was confirmed that the identification of the various orbital lymphoid infiltrates becomes more distinct when immunological techniques are added to the clinical and histopathological methods of investigation. Multidisciplinary cooperation leads to further improvement of diagnosis and treatment of ocular adnexal lymphoproliferative disease.

Adult↗
PubMed1983

Intraventricular methotrexate therapy of leptomeningeal metastasis from breast carcinoma.

Treatment results of leptomeningeal metastasis are reported in 33 breast cancer patients. They were divided into three groups: group 1, 19 patients, received intraventricular methotrexate (MTX) with doses based on CSF MTX levels; group 2, 6 patients, received whole brain radiation followed by a course of MTX given by lumbar punctures; group 3, 8 patients, was not treated. Median survival in group 1 was 6 months; 25% survived 1 year or more. Median survival (1 to 2 months) in groups 2 and 3 was significantly shorter. Neurologic improvement was seen in an average time of 4 weeks in about 80% of group 1 patients. Two of group 2 patients improved at 3 weeks, and all group 3 patients deteriorated. Carcinomatosis caused death significantly less often in group 1 than in the other groups.

Adult↗
PubMed1982

Pseudo-tumours, a clinical concept.

UNLABELLED: Space-filling lesions in the orbit can be divided into 3 large groups: 1. Acute and subacute inflammatory processes (bacterial, fungal, parasitic, foreign body). 2. Benign and malignant tumours, true new growths. 3. Pseudo-tumours. The conditions in group 3 are also space-filling lesions, but have the characteristics of a chronic, infiltrating, inflammatory process. In 11% of 340 consecutive patients in the Orbital Centre in Amsterdam this diagnosis was made. The group is far from uniform: the dominant cell type varies from one case to another. Usually lymphocytic cells are dominant; if these are arranged in follicles the lesion is called a pseudo-lymphoma. This condition can gradually leave the province of the pseudo-tumours and begin to show the characteristics of a malignant or non-Hodgkin lymphoma. Sometimes the inflammatory character of the lymphoid infiltration is dominant, as in myositis, sclerotenonitis and infiltrative Graves' ophthalmopathy; these conditions are clearly related to immunological processes. Attempts to classify all pseudo-tumours on a pathological basis have remained rather vague. CLINICAL PICTURE: rapid development (2-6 months); pain and oedema are present, but these are not the principal symptoms; secondary features are: disorders of motility, visual loss and other symptoms. Bilaterality usually indicates the presence of a systemic disease.

Connective Tissue Diseases↗
PubMed1982

Cytogenetic studies on four cases of non-endemic Burkitt lymphoma.

Cytogenetic studies carried out on four children with non-endemic Burkitt lymphoma showed: 1) Two with the typical translocation t(8;14)(q24;q23); 2) one with a variant t(2;8)(p11;q24); and 3) one with apparently normal chromosomes 8 and 14. Additional chromosomal variation was present in all four patients. Two were shown to have a duplication of part of the long arm of chromosome 1 (1q23 leads to 1q32 and 1q23 leads to 1q42). Epstein Barr virus studies on two patients showed that one was positive and the other negative. A comparison of these results with other non-endemic cases in the literature has been made revealing a wider range of chromosomal variation than has been hitherto reported for endemic cases. The finding that chromosome 8 is also involved in the variant translocations in Burkitt lymphoma suggests that its changes may contribute more to the tumour development than the than the 14q+.

Bone Marrow↗
PubMed1981

A malignant tumor arising from interdigitating cells; light microscopical, ultrastructural, immuno-and enzyme-histochemical characteristics.

A tumor in a 37 years old male is described in which the tumor cells appeared to be derived from interdigitating cells normally found in the T-cell area of lymph nodes. The patient presented with superior vena caval obstruction due to a mediastinal mass, followed by lymph node enlargement and skin lesions leading to death within 4 months. The tumor cells lacked immune markers for lymphocytic cells. They showed Ia-like antigens and high adenosine triphosphatase activity, while acid phosphatase and alpha-naphthyl acetate esterase activity was absent. Their fine morphology was strikingly similar to that of interdigitating cells. A combination of these data led us to the conclusion that this tumor represents a specific subtype of the tumors derived from the mononuclear phagocyte system, namely a sarcoma of interdigitating cells.

Adult↗
PubMed1981

Quantitative analysis of the nuclear area variation in benign and malignant breast cytology specimens.

The diagnostic value of the nuclear size for discriminating between benign and malignant breast lesions was studied by karyometric analysis of the nuclear area in May-Grünwald-Giemsa-stained imprints from 173 benign and 103 malignant breast lesions. The mean nuclear area, the maximum nuclear area and the percentage of nuclei larger than 200 sq micrometer discriminated between about 60% of the breast carcinomas and 95% of the benign lesions. The coefficient of variation had considerably less discriminatory power and also showed the lowest correlation with the mean nuclear area. A weak negative correlation was found between the mean nuclear area and the age of the patients with benign lesions. Carcinomas with small nuclei (area less than 110 sq micrometer) were found more frequently in patients with negative lymph nodes than in patients with positive nodes. Additional parameters for nuclear shape and texture may lead to a further objectification of nuclear pleomorphism in breast cytology specimens.

Adult↗
PubMed1980

Non-Hodgkin's lymphoma. Immunohistochemical and electron microscopical findings in relation to lightmicroscopy. A study of 74 cases.

From 74 patients with non-Hodgkin's lymphoma (NHL), fresh biopsy material from involved tissue was investigated histochemically and by light and electron microscopy. The results were compared with the light microscopical conclusions based on three currently utilized classifications, namely of Rappaport, Lukes, and Lennert. The separate groups of low-grade NHL appeared to consist of homogeneous cell populations, both in immunohistological as well as in enzymehistochemical and electron microscopical sense. On the contrary, high-grade NHL constituted a heterogeneous group in which supplementary (immunohistochemical, enzymehistochemical, and electron microscopical) investigation is very useful. All NHLs reacted with anti-human lymphocyte serum, including the so-called histiocytic NHLs according to the Rappaport classification, which proves their lymphocytic origin. Consequently the prefix "histiocytic" should be altered in a morphogenetically correct way. Without exception the B-cell NHLs were characterized by the presence of monoclonal immunoglobulin on the cell membrane and/or intracytoplasmically. In a part of the immunologically non-T-/non-B-/("null cell") NHLs, a B-cell origin was indicated by the presence of ATPase and/or 5' nucleotidase and a T-cell origin by the presence of dot-like acid phosphatase.

Adenosine Triphosphatases↗
PubMed1978

Surface markers and functional properties of non-Hodgkin's lymphoma cells in relation to histology.

Cells from 32 adult patients with non-Hodgkin's lymphoma were studied with respect to surface markers and functional properties in short-term culture. Twenty-six lymphomas were of B-cell origin, including all nodular and diffuse lymphocytic lymphomas. Three tumors were of T-cell origin (one histiocytic lymphoma and two undifferentiated lymphomas). In the remaining three cases (histiocytic lymphomas) the immunological nature of the tumor cells could not be determined. All reactivity to mitogenic stimuli of cells from B-cell lymphomas was due to residual normal T cells. In follicular lymphocytic lymphomas more reactive T cells prevailed among the malignant B cells than in diffuse lymphocytic lymphomas. Heterogeneity among B-cell lymphomas was indicated by differences in intensity of fluorescence with anti-Ig reagents and in stimulatory capacity in mixed lymphocyte culture. T-cell lymphomas were characterized by high percentages of T cells together with impaired responses to stimuli. The results of immunological studies correlated well with the histological classifications of Rappaport, Lukes and Lennert.

Adult↗
PubMedDate not supplied

Malignant histiocytosis and related tumors. A clinicopathologic study of 42 cases using cytological, histochemical and ultrastructural parameters.

Light and electron microscopical, immunohistochemical and clinical characteristics in 42 cases of malignant neoplasms, arising from true histiocytes, are described. These were separated in a lymphoma-like subtype, called true histiocytic lymphoma (29 patients) and a disseminated variant, called malignant histiocytosis (9 patients). In addition 4 related histiocytic tumors are discussed, including 2 tumors arising from interdigitating cells. Sinus pattern and cytologic features, especially 'window' nuclei, are emphasized as diagnostic criteria. Erythrophagocytosis was not a constant finding. Electron microscopic features, presence of acid phosphatase, acid alpha-naphthylacetate esterase, lysozyme, alpha 1-antitrypsin, alpha 1-antichymotrypsin, Ia-antigen and absence of B- and T-cell markers, were important in establishing the histiocytic nature or excluding a non-histiocytic tumor. A distinct male predominance existed (male:female = 2.5:1) with a higher relapse free period in females (p = 0.032). A high number of mitotic figures appeared to be a favourable sign, p = 0.020 and 0.019, for remission rate and relapse free period respectively. The degree of cell differentiation and the immunohistochemical pattern did not show a correlation with remission and relapse free period. Extranodal involvement and the presence of short profiles of endoplasmic reticulum were prognostically unfavourable signs. True histiocytic lymphomas showed a higher remission rate (p = 0.041) and relapse-free period (p = 0.017) than malignant histiocytosis.

Adolescent↗