Biomedical subjects

Pascal Madeleine

Publications and source records attributed to Pascal Madeleine.

At least 19 recordsLinked to original sources

PubMed2006

The change in spatial distribution of upper trapezius muscle activity is correlated to contraction duration.

The aim of the study was to confirm the hypothesis that the longer a contraction is sustained, the larger are the changes in the spatial distribution of muscle activity. For this purpose, surface electromyographic (EMG) signals were recorded with a 13 x 5 grid of electrodes from the upper trapezius muscle of 11 healthy male subjects during static contractions with shoulders 90 degrees abducted until endurance. The entropy (degree of uniformity) and center of gravity of the EMG root mean square map were computed to assess spatial inhomogeneity in muscle activation and changes over time in EMG amplitude spatial distribution. At the endurance time, entropy decreased (mean+/-SD, percent change 2.0+/-1.6%; P<0.0001) and the center of gravity moved in the cranial direction (shift 11.2+/-6.1mm; P<0.0001) with respect to the beginning of the contraction. The shift in the center of gravity was positively correlated with endurance time (R(2)=0.46, P<0.05), thus subjects with larger shift in the activity map showed longer endurance time. The percent variation in average (over the grid) root mean square was positively correlated with the shift in the center of gravity (R(2)=0.51, P<0.05). Moreover, the shift in the center of gravity was negatively correlated to both initial and final (at the endurance) entropy (R(2)=0.54 and R(2)=0.56, respectively; P<0.01 in both cases), indicating that subjects with less uniform root mean square maps had larger shift of the center of gravity over time. The spatial changes in root mean square EMG were likely due to spatially-dependent changes in motor unit activation during the sustained contraction. It was concluded that the changes in spatial muscle activity distribution play a role in the ability to maintain a static contraction.

Adult↗

PubMed2006

Experimental muscle pain changes the spatial distribution of upper trapezius muscle activity during sustained contraction.

OBJECTIVE: To investigate the effect of local excitation of nociceptive muscle afferents on the spatial distribution of muscle activity. METHODS: Surface electromyographic (EMG) signals were recorded from the upper trapezius muscle of 10 healthy volunteers with a 5 x 13 electrode grid during 90-s isometric contractions before, during, 15 and 30 min after intramuscular injection of hypertonic (painful) or isotonic (non-painful) saline. From the multi-channel EMG recordings, two-dimensional maps of root mean square and mean power frequency were obtained. The centre of gravity of the root mean square map was used to quantify global changes in the spatial distribution of muscle activity. RESULTS: During sustained contractions, average root mean square increased, average mean frequency decreased and the centre of gravity moved cranially. During experimental muscle pain, compared to before injection, the average root mean square decreased and there was a caudal shift of the centre of gravity. Fifteen minutes after the painful injection the centre of gravity returned to its original position. CONCLUSIONS: Short-term dynamic reorganization of the spatial distribution of muscle activity occurred in response to nociceptive afferent input. SIGNIFICANCE: The study furnishes an extension of the pain adaptation model indicating heterogeneous inhibition of muscle activity.

Adult↗

PubMed2006

Effects of electromyographic and mechanomyographic biofeedback on upper trapezius muscle activity during standardized computer work.

The purpose of this laboratory study was to investigate the effects of surface electromyography (EMG)- and mechanomyography (MMG)-based audio and visual biofeedback during computer work. Standardized computer work was performed for 3 min with/without time constraint and biofeedback in a randomized order. Biofeedback was given on the basis of an individual preset threshold value for the right trapezius EMG and MMG signal and a time factor (repetition of events above the threshold). The duration of muscle activity above the preset threshold, the right trapezius EMG and MMG root mean square (RMS) values as well as the work performance in terms of number of completed graph/mouse clicks/errors, the rating of perceived exertion (RPE) and the usefulness of the biofeedback were assessed. The duration of muscle activity above the threshold was significantly lower with MMG compared with EMG as source of biofeedback (p < 0.05). Biofeedback led to a significant decrease in the right trapezius EMG RMS, lower RPE and decreased number of errors and mouse clicks, but also decreased number of completed graphs (p < 0.05). Audio and visual biofeedbacks were as effective. MMG-based biofeedback is a potential reliable alternative to EMG in ergonomics. A lowering of the trapezius muscle activity may contribute to diminish the risk of work related musculoskeletal disorders development.

Adult↗

PubMed2006

Two-dimensional spatial distribution of surface mechanomyographical response to single motor unit activity.

In order to better understand the mechanisms of generation of mechanomyography (MMG) signals, the two-dimensional distribution of surface MMG produced by the activity of single motor units was analyzed by a novel two-dimensional recording method. Motor unit action potentials were identified from intramuscular electromyographic (EMG) signals and used to trigger the averaging of MMG signals detected over the tibialis anterior muscle of 11 volunteers with a grid of 5x3 accelerometers (20-mm inter-accelerometer distance). The intramuscular wires were inserted between the first and second accelerometer in the middle column of the grid, proximal to the innervation zone. The subjects performed three contractions with visual feedback of the intramuscular EMG signals. In each contraction, a new motor unit was recruited at the minimum stable discharge rate (mean+/-S.D., N = 11 subjects, 7.3+/-2.3 pulse/s), resulting in torque of 2.4+/-2.8% of the maximal voluntary contraction (MVC), 4.6+/-2.7% MVC, and 6.3+/-3.1% MVC (all different, P < 0.01). For 23 out of 33 detected motor units, it was possible to extract the motor unit surface acceleration map (MUAM). A negative MUAM peak (-2.7+/-2.2 mm/s2) was detected laterally and a positive MUAM peak (4.1+/-2.4 mm/s2) medially (P < 0.001). The time-to-peak was shorter in the medial part of the muscle (2.9+/-0.4 ms) than in the other locations (3.4+/-0.5 ms, P < 0.001). The double integrated signals (muscle displacement) indicated negative deflection in the lateral part and inflation close to the tibia bone. The maps of acceleration showed spatial dependency in single motor unit MMG activities. The technique provides a new insight into motor unit contractile properties.

Acceleration↗

PubMed2006

Spatial and force dependency of mechanomyographic signal features.

The aim was to investigate with a novel technique the spatial inhomogeneity in surface mechanomyographic (MMG) response to muscle contraction at varying force levels. MMG signals were detected over the dominant tibialis anterior muscle of 10 volunteers using a 5 x 3 grid of accelerometers. The subjects performed 3 s long isometric contractions at forces ranging from 0% to 100% of the maximal force (10% increment) in a randomised order. From the two-dimensional MMG recordings, maps of absolute and normalized temporal and spectral MMG descriptors were obtained. The centroid and entropy of these maps were computed to describe the spatial centre of activity and degree of homogeneity, respectively. MMG absolute amplitude did not depend on location over the muscle while normalized amplitude did and the centroid shifted with increasing force. Amplitude increased with force and its entropy decreased. Absolute and normalized spectral variables depended on location over the muscle and their centroid shifted with increasing force. In addition, the dependency of absolute and normalized spectral variables on force was affected by location. These results highlight limitations when using single-channel MMG features for the assessment of motor unit control strategies, due to a substantial effect of position on the relation between force and MMG characteristics.

Acceleration↗

PubMed2006

Gender effects on trapezius surface EMG during delayed onset muscle soreness due to eccentric shoulder exercise.

The purpose of this study was to investigate gender-specific motor control strategies during eccentric exercise and delayed onset muscle soreness (DOMS) in the shoulder region. Twelve healthy males and females participated in the study. Eccentric shoulder exercises were conducted on the dominant shoulder while the other side served as control. The exerted force, range of shoulder elevation, rating of perceived exertion, pain intensity, and surface electromyography (EMG) from the trapezius muscles were recorded and analyzed. A significant decrease in exerted force during exercise was only found in males despite similar rating of perceived exertion among genders. During eccentric exercise: males showed increasing root mean square (RMS) of the EMG while a decrease occurred for females, no difference between genders in mean power frequency of the EMG were seen. During static and dynamic contractions: no differences between genders in pain intensity or RMS were observed; RMS of the exercised side were lower than that of the control side (P<0.05) at 24 h after exercise. The results indicated a more prominent muscle fatigue resistance in females compared with males and mobilization of different muscle activation strategies during eccentric exercise. A protective adaptation to DOMS, i.e. decrease in RMS values was found with no gender differences.

Adaptation, Physiological↗

PubMed2006

A comparison between mechanomyographic condenser microphone and accelerometer measurements during submaximal isometric, concentric and eccentric contractions.

The aim of this study was to compare mechanomyogram (MMG) recorded by a condenser microphone (MIC) and an accelerometer (ACC) during submaximal isometric, concentric and eccentric contractions in 14 males. The maximal voluntary force (MVC) of the biceps brachii was measured. The subjects were asked to do short duration isometric, concentric and eccentric contraction at 10%, 30%, 50%, 70% MVC twice. For the concentric and eccentric contraction, the subject bent his arm for 3s (concentric) then held it for 3s and extended (eccentric) during 3s. The normalized root mean square (RMS) and mean power frequency (MPF) increased linearly with increased force for both transducers. There was a correlation between MIC MPF and ACC MPF at 10%, 30%, 50% MVC, and between MIC RMS and ACC RMS at 30% MVC during isometric contractions. There was significantly higher MPF for the ACC than for the MIC in concentric and eccentric modes, while the RMS did not differ among transducers in the three contraction modes. The RMS and MPF values coefficient of variations were significantly larger during anisometric contractions compared with isometric contractions and were lower for the accelerometer than for the microphone. The present results obtained during isometric, concentric and eccentric contractions of increased intensity showed that the information contained in microphone- and accelerometer-based MMG signals is different despite similar trends. It can be concluded that at low-moderate movement velocity, concentric contractions can be investigated by means of accelerometer and microphone.

Adult↗

PubMed2006

Spectral moments of mechanomyographic signals recorded with accelerometer and microphone during sustained fatiguing contractions.

The aim of this study was to analyse the trends of the first three power spectral moments of the mechanomyogram (MMG) signal recorded by a microphone (MMG(MIC)) and an accelerometer (MMG(ACC)) during sustained contractions. MMG signals were recorded from the biceps brachii muscle in 14 healthy male subjects during a 3 min isometric elbow flexion at 30% of the maximal voluntary contraction. MMG absolute and normalised root mean square (RMS), mean power frequency (MNF), power spectral variance (Mc2), and skewness (mu3) were computed. For both MMG(MIC) and MMG(ACC), absolute and normalised RMS and Mc2 increased while MNF and mu3 decreased with contraction time (P<0.001). The rates of change of RMS over time were significantly correlated (P<0.001) for MMG(MIC) and MMG(ACC) but not correlated for spectral moments. The coefficient of variation of RMS was higher for MMG(MIC) than for MMG(ACC), while the opposite was observed for mu3 (P<0.05). It was concluded that higher order spectral moments of the MMG signal change during sustained contraction, indicating a complex modification of the shape of the power spectrum and not just scaling of the bandwidth. This is most likely due to the additional motor unit recruitment with fatigue and to the non-linear summation of motor unit contributions to the signal. Moreover, the characteristics of MMG signals recorded with microphones and accelerometers have important differences, which should be taken into account when comparing results from different studies.

Adult↗

PubMed2006

Hypoalgesia in the referred pain areas after bilateral injections of hypertonic saline into the trapezius muscles of men and women: a potential experimental model of gender-specific differences.

OBJECTIVE: The study was to assess the local pain intensity, referred pain patterns, and changes in the pressure pain thresholds of the local and referred pain areas following bilateral injections of hypertonic saline into trapezius muscles and to delineate gender differences in pain profile and the possible modulation of pressure pain thresholds. METHODS: Fifteen healthy men and women each participated. Pain intensity and areas were assessed after bilateral injections. The pressure pain thresholds in the trapezius and posterolateral neck muscles (referred pain areas) were measured before and 7.5 and 15 minutes postinjection. RESULTS: The time to maximal pain intensity was significantly shorter after the second injection than after the first injection. Patients exhibited a bilaterally symmetrical distribution of pain areas that included the neck to shoulder angle and the posterolateral neck. Bilateral injections significantly elevated pressure pain thresholds in the right and left posterolateral neck muscles 7.5 and 15 minutes postinjection. In men but not women, the pressure pain thresholds measured in the posterolateral neck muscles 7.5 and 15 minutes after the second injection were higher than those after the first injection and also higher than those after the second injection in women, men tolerated more pressure than women at all time points. DISCUSSION: These findings indicate that both excitatory and inhibitory mechanisms modulate pain response characteristics in bilateral neck-shoulder pain conditions and suggest that there may be a more potent inhibitory control mechanism in men than women. This experimental model may be potentially used to detect gender differences in descending inhibition.

Adult↗

PubMed2005

Enhanced temporal summation of pressure pain in the trapezius muscle after delayed onset muscle soreness.

Temporal summation of muscle pain is an important factor in musculoskeletal pain as central integration of repetitive nociceptive input can be facilitated in musculoskeletal pain patients. The aim of this study is to evaluate changes in temporal summation of pressure pain after induction of delayed onset muscle soreness (DOMS) of the trapezius muscle. Sixteen healthy volunteers participated in the study. Temporal summation of pain was induced by sequential pressure stimulation by a computer-controlled algometer. Sequential stimulation consisting of ten stimuli (at pressure pain threshold intensity) was applied over the trapezius muscle. Stimulus duration was 1 s and inter-stimulus intervals (ISI) were 1, 5, 10, and 30 s, respectively. The pain was rated on a continuous visual analogue scale (VAS, 10 cm) after each stimulus and normalised to the VAS score from the first stimulus. DOMS was induced in the right trapezius muscle by eccentric shoulder exercises while the left trapezius muscle served as control. Temporal summation of pressure evoked pain was measured before and 24 h after the exercise. At 24 h after exercise, soreness intensity during shoulder elevation was 3.7+/-0.2 cm, while no soreness was observed on the control side. When sequential pressure stimulation was applied to the DOMS muscle, VAS scores for 1 s ISI progressively increased to a higher level than before exercise (VAS increase for the last stimulus: 0.8+/-0.2 cm vs. 0.6+/-0.1 cm, P<0.05), while VAS scores for ISI 5, 10, and 30 s were not increased. On the control side, significant increases in VAS scores was observed for all ISIs but not affected by contralateral DOMS. Facilitation of temporal summation for 1 s ISI indicated that DOMS may increase the central excitability besides involving peripheral sensitisation. During DOMS there was no potential for further nociceptor sensitisation by repeated noxious pressure stimuli, which may account for the diminishment of temporal summation evoked by pressure stimuli with ISI 5, 10, and 30 s. These data indicate that muscle soreness might facilitate the central components of temporal summation to mechanical stimulation.

Adult↗

PubMed2005

Gradual enlargement of human withdrawal reflex receptive fields following repetitive painful stimulation.

Dynamic changes in the topography of the human withdrawal reflex receptive fields (RRF) were assessed by repetitive painful stimuli in 15 healthy subjects. A train of five electrical stimuli was delivered at a frequency of 3 Hz (total train duration 1.33 s). The train was delivered in random order to 10 electrode sites on the sole of the foot. Reflexes were recorded from tibialis anterior, soleus, vastus lateralis, biceps femoris, and iliopsoas (IL). The RRF changes during the stimulus train were assessed during standing with even support on both legs and while seated. The degree of temporal summation was depending on stimulation site. At the most sensitive part of the RRF, a statistically significant increase in reflex size was seen after two stimuli while four stimuli were needed to observe reflex facilitation at less sensitive electrode sites. Hence, the region from which reflexes could be evoked using the same stimulus intensity became larger through the train, that is, the RRF was gradually expanding. Reflexes evoked by stimuli four and five were of the same size. No reflex facilitation was seen at other stimulus sites outside the RRF. In all muscles except in IL, the largest reflexes were evoked when the subjects were standing. In the ankle joint, the main withdrawal pattern consisted of plantar flexion and inversion when the subjects were standing while dorsi-flexion was prevalent in the sitting position. Up to 35 degrees of knee and hip flexion were evoked often leading to a lift of the foot from the floor during standing. In conclusion, a gradual expansion of the RRF was seen in all muscles during the stimulus train. Furthermore, the motor programme task controls the reflex sensitivity within the reflex receptive field and, hence, the sensitivity of the temporal summation mechanism.

Analysis of Variance↗

PubMed2005

Gender-specific differences in electromyographic changes and perceived pain induced by experimental muscle pain during sustained contractions of the upper trapezius muscle.

The aim of the study was to investigate gender-specific differences in the pain-induced alterations of muscle activity during sustained contractions of the upper trapezius muscle. Surface electromyographic (EMG) signals were recorded bilaterally from the upper trapezius muscles of healthy volunteers (10 men and 9 women). The subjects held the arms at 90 degrees abduction for 5 s (short contraction) and 3 min (sustained contraction). These contractions were performed before, during, and after injection of 0.5 ml of hypertonic saline (5.8%) bilaterally in the upper trapezius muscles. Injection of isotonic saline (0.9%) served as control. The root mean square (RMS) and mean power frequency (MPF) were computed from the EMG signals and normalized to the values obtained during maximal voluntary contractions. In the short contractions, injection of hypertonic saline, but not isotonic saline, induced a less pronounced decrease in RMS in women than men, and a significant decrease in MPF in men but not women. In the sustained contractions, RMS slope increased and MPF slope decreased (less negative) with muscle pain in men but not women. It was concluded that the pain-induced changes in motor control strategies during sustained contractions differ in men and women, which may partly explain gender differences in the incidence of musculoskeletal pain.

Adult↗

PubMed2005

Delayed onset muscle soreness in neck/shoulder muscles.

The aim of the present study is to: (1) induce delayed onset muscle soreness (DOMS) in the neck and shoulder muscles; (2) compare the pressure pain sensitivity of muscle belly with that of musculotendinous tissue after DOMS; (3) examine the gender differences in the development of DOMS. An eccentric shoulder exercise was developed to induce DOMS on neck/shoulder muscles using a specially designed dynamometer. Eccentric shoulder contraction consisted of 5 bouts, each bout lasted 3min, with 3min rest period between each bout. The right shoulder was elevating against a downward pressure force of 110% maximal voluntary contraction force exerted by the dynamometer. Pressure pain thresholds (PPT) of 11 sites (seven sites measured were muscle belly and four sites were myotendinous area) on neck/shoulder region were measured before, immediately after, 24 and 48h after exercise. Pain intensity, pain area and index of McGill pain questionnaire were assessed and all were increased after exercise. DOMS was induced in the shoulder muscles. PPT was significantly decreased and reached lowest values at 24h. The muscle belly sites are more sensitive to pain than the musculotendinous sites. No gender differences were found in any of the parameters used to assess the development of DOMS. DOMS did not distribute evenly in the neck/shoulder region. Soreness after exercise in the neck and shoulder seems not to be among the conditions that produce predominant musculoskeletal pain in females.

Adult↗

PubMed2005

Gender differences in pain modulation evoked by repeated injections of glutamate into the human trapezius muscle.

Gender differences in pain habituation, temporal summation, and pressure hyperalgesia evoked by repeated injections of glutamate into the dominant trapezius muscle were investigated. The glutamate-evoked muscle pain intensity and pressure pain threshold (PPT) were assessed. The PPTs were measured bilaterally in the trapezius muscles (local pain area) and posterolateral neck muscles (referred pain area) after glutamate injection in healthy and age-matched males and females (each n=14). Two glutamate injections (0.4 ml, 2M each) were injected with an interval of 5 min. One injection of glutamate (0.4 ml, 2M) served as a control. Males, but not females, rated the second injection (maximal pain intensity) significantly less painful than the first injection. The area under the visual analogue scale pain curve of the second injection was significantly larger than the first injection in females. Repeated glutamate injections, but not one-glutamate injection, significantly decreased PPTs in the local pain area, with no significant gender differences. No PPTs changes were observed either in the contralateral trapezius muscle or bilaterally in the referred pain areas in either sex. These results suggest that a less efficient pain habituation and a greater susceptibility to the development of temporal summation of muscle pain in females, but not in males, might be one of the contributing factors to the higher incidence of neck shoulder pain in females. In addition, the reduction of PPTs in the local pain area evoked by intramuscular glutamate injection may represent an early process of peripheral pressure hyperalgesia, which is most likely gender independent.

Adult↗

PubMed2004

Voluntary low-force contraction elicits prolonged low-frequency fatigue and changes in surface electromyography and mechanomyography.

Controversies exist regarding objective documentation of fatigue development with low-force contractions. We hypothesized that non-exhaustive, low-force muscle contraction may induce prolonged low-frequency fatigue (LFF) that in the subsequent recovery period is detectable by electromyography (EMG) and in particular mechanomyography (MMG) during low-force rather than high-force test contractions. Seven subjects performed static wrist extension at 10% maximal voluntary contraction (MVC) for 10 min (10%MVC10 min). Wrist force response to electrical stimulation of extensor carpi radialis muscle (ECR) quantified LFF. EMG and MMG were recorded from ECR during static test contractions at 5% and 80% MVC. Electrical stimulation, MVC, and test contractions were performed before 10%MVC10 min and at 10, 30, 90 and 150 min recovery. In spite of no changes in MVC, LFF persisted up to 150 min recovery but did not develop in a control experiment omitting 10%MVC10 min. In 5% MVC tests significant increase was found in time domain of EMG from 0.067+/-0.028 mV before 10%MVC10 min to 0.107+/-0.049 and 0.087+/-0.05 mV at 10 and 30 min recovery, respectively, and of the MMG from 0.054+/-0.039 ms(-2) to 0.133+/-0.104 and 0.127+/-0.099 ms(-2), respectively. No consistent changes were found in 80% MVC tests. In conclusion, non-exhaustive low-force muscle contraction resulted in prolonged LFF that in part was identified by the EMG and MMG signals.

Adult↗

PubMed2004

Dynamic shoulder dynamometry: a way to develop delay onset muscle soreness in shoulder muscles.

A dynamic shoulder dynamometer has been designed to develop a vertical downward force over a measured range of motion, i.e. scapular elevation. The dynamometer is force-controlled to apply a required force within the range of motion. Calibration procedures were performed to assess the reliability of the force and displacement measurements. The system is designed to operate in isometric or eccentric conditions. For eccentric exercise the dynamometer has, for the first time, provided possibilities to elicit delay onset muscle soreness in shoulder muscles. In future, the apparatus will enable investigations of sensory-motor interactions in the shoulder region.

Biomechanical Phenomena↗

PubMed2004

Quantitative posturography in altered sensory conditions: a way to assess balance instability in patients with chronic whiplash injury.

OBJECTIVES: To quantify neck mobility and posture with and without various postural perturbations. DESIGN: A multivariable 2-group study with repeated measures and treatments. SETTING: A human performance laboratory. PARTICIPANTS: Eleven patients with chronic whiplash injury (mean age, 33.3+/-6.7 y; weight, 73.4+/-11.4 kg; height, 173.3+/-7.2 cm) with a sex- and age-matched control group (mean age, 33.1+/-6.8 y; weight, 68+/-12.5 kg; height, 171.5+/-6.3 cm). INTERVENTIONS: Neck mobility and the effects of postural perturbations affecting the visual, vestibular, cutaneous, proprioceptive, and nociceptive systems were measured. MAIN OUTCOME MEASURES: Active range of motion, neck position sense, and postural activity. RESULTS: We found significantly reduced neck mobility and increased postural activity in the patient group compared with the control group. In patients, there was significantly greater postural activity with eyes closed, eyes open and speaking, and eyes closed with Achilles' tendons vibrations compared with eyes open with no vibrations. In the controls, there was no significant effect of experimental muscle pain on postural activity. CONCLUSIONS: Patients with chronic whiplash injury had a protective response to neck movement and different tuning, sequencing, and execution of the postural synergies probably because of excessive reliance on visual input despite a possible deficit and altered vestibular and/or proprioceptive activity. In healthy volunteers, the pain induced by a single bolus injection of hypertonic saline was probably too limited in intensity and spreading to decrease postural stability.

Adult↗

PubMed2004

Simultaneous modulation of the exteroceptive suppression periods in the trapezius and temporalis muscles by experimental muscle pain.

OBJECTIVE: To investigate whether the exteroceptive suppression (ES) periods in the trapezius and temporalis muscles could be simultaneously modulated by either experimental trapezius muscle pain or temporalis muscle pain. METHODS: Fourteen healthy volunteers participated in two sessions with randomised injections of either hypertonic or isotonic saline into the right trapezius muscle or right temporalis muscle. The pain intensity was continuously scored on a 10 cm electronic visual analogue scale. During muscle contraction, the early (ES1) and late (ES2) reflex in the temporalis and late (ES2) reflex in the trapezius elicited by electrical stimulation of the right infraorbital nerve were recorded pre-, during-, and post-injection. RESULTS: Hypertonic saline injection induced local muscle pain and referred pain with the maximal pain intensity of 6.3+/-0.5 cm in the right trapezius and 6.7+/-0.6 cm in the right temporalis muscles. Injection of either hypertonic or isotonic saline into the right trapezius muscle was associated with significantly shortened ES2 duration and offset time in the injected right trapezius muscle. Only injection of hypertonic saline into the right trapezius muscle could significantly reduce the ES2 offset time and decrease the degree of suppression of ES2 in the right temporalis and left trapezius muscles. Injection of either hypertonic or isotonic saline into the right trapezius muscle evoked no changes in ES2 parameters in the left temporalis muscle. Injection of hypertonic saline, but not isotonic saline, into the right temporalis muscle caused a significantly shorter ES2 offset time and shorter ES2 duration bilaterally in the temporalis and trapezius muscles. CONCLUSIONS: The findings of the present study provide evidence for a bi-directional effects reflecting convergence of muscle afferents from the trigeminal and upper cervical neural systems in humans, which may partly underlie the manifestations of pain referral between these two areas.

Adult↗