Biomedical subjects

Paul J Ellis

Publications and source records attributed to Paul J Ellis.

3 recordsLinked to original sources

PubMed2003

Beamstop with integrated X-ray sensor.

A versatile beamstop with an integrated sensor has been developed at the Stanford Synchrotron Radiation Laboratory (SSRL) using non-specialized components. A diameter of 1.5 mm was achieved using a commercial subminiature surface mount PIN diode (Phillips BAP64) molded into a tungsten epoxy composite cup. The cup is supported on a thin fiberglass arm with printed circuit traces to transmit the signal from the diode. The assembly has an active area of approximately 100 micro m in diameter. As the diode is encapsulated in plastic, the response diminishes with decreasing energy but is still useful at 6 keV.

Journal Article↗

PubMed2002

Crystallization of Pichia pastoris lysyl oxidase.

A copper-containing amine oxidase (PPLO) from the yeast Pichia pastoris has been purified and crystallized in two forms. PPLO is a glycoprotein. The molecular mass from SDS-polyacrylamide gels is 112 kDa, consistent with 20% glycosylation by weight (the calculated molecular weight of the polypeptide is 89.7 kDa). Orthorhombic crystals belonging to space group P2(1)2(1)2(1), with unit-cell parameters a = 163.7, b = 316.1, c = 84.0 A, diffract to 2.65 A resolution. Monoclinic crystals belonging to space group C2, with unit-cell parameters a = 248.4, b = 121.1, c = 151.8 A, beta = 124.6 degrees, diffract to 1.65 A resolution. Native data have been recorded from each crystal form at 100 K using synchrotron radiation. A self-rotation function for the monoclinic crystal form reveals the presence of a non-crystallographic twofold axis perpendicular to the crystallographic twofold axis, consistent with the presence of two dimers in the asymmetric unit.

Crystallization↗

PubMed2002

Blu-Ice and the Distributed Control System: software for data acquisition and instrument control at macromolecular crystallography beamlines.

The Blu-Ice and Distributed Control System (DCS) software packages were developed to provide unified control over the disparate hardware resources available at a macromolecular crystallography beamline. Blu-Ice is a user interface that provides scientific experimenters and beamline support staff with intuitive graphical tools for collecting diffraction data and configuring beamlines for experiments. Blu-Ice communicates with the hardware at a beamline via DCS, an instrument-control and data-acquisition package designed to integrate hardware resources in a highly heterogeneous networked computing environment. Together, Blu-Ice and DCS provide a flexible platform for increasing the ease of use, the level of automation and the remote accessibility of beamlines. Blu-Ice and DCS are currently installed on four Stanford Synchrotron Radiation Laboratory crystallographic beamlines and are being implemented at sister light sources.

Crystallography, X-Ray↗