Biomedical subjects

Pei Wang

Publications and source records attributed to Pei Wang.

60 records · Page 4Linked to original sources

PubMed2002

[The dose-response relationship study between the quantitative morphological stereology on thyroid and different iodine doses in mice].

OBJECTIVE: To study the dose-response relationship between the quantitative morphological stereology on thyroid and different iodine doses in mice. METHODS: Weaning Kunming mice were randomly divided into seven groups. The mice were fed for 100 days with distilled water containing different KIO3 concentrations, i.e. 50, 250, 500, 1 000, 1 500, 2 000, and 3 000 microgram/L respectively. The 50 microgram/L (proper iodine concentration) group was control group, and the groups of 250 approximately 3 000 microgram/L were high iodine groups. The stereology parameters of thyroid follicle and follicular cavities were measured with HPIAS-1000 (High Resolution Pathological Image & word Analysis System). The stereology parameters included mean surface, volume on area, volume on circumference, specific surface, numerical density on area, spherical factor, the percentage of mean surface and mean volume of the follicular epithelial cell in thyroid follicle was further calculated. RESULTS: Positive correlations was observed between the thyroid absolute and relative weight, goiter rate and different iodine doses. And the thyroid absolute and relative weight of mice in the 250 microgram/L group was significantly different from that in 50 microgram/L group. The goiter rate of mice in different high iodine groups was in conformity with that of epidemiological investigation. The goiter rate of mice in 500 microgram/L group was different from that in 50 microgram/L group. Positive correlations were observed between mean surface, volume on area, volume on circumference, spherical factor and iodine doses, but the negative correlations were observed between numerical density on area, specific surface, the percentage of mean surface and mean volume of the follicular epithelial cell in thyroid follicle and iodine doses. CONCLUSIONS: When Iodine doses are between 250 approximately 3 000 microgram/L, the dose-response relationship was observed between the morphological stereology parameters of thyroid follicle and follicular cavities and iodine doses, and when the dose of iodine is 250 microgram/L, it is possible to induce colloid goiter of mice. The goiter rates of mice resulted by different high iodine doses were in conformity with that of the epidemiological investigation of people.

Animals↗

PubMed2002

[The influence of different nutritional support routes on the intestinal mucosal epithelial cell cycle in burned rats].

OBJECTIVE: To explore the influence of different nutritional support routes on the intestinal mucosal epithelial cell cycle in burned rats. METHODS: Sixty-six Wistar rats inflicted with 30% TBSA III degree burns on the back were employed as the model and were randomly divided into enteral feeding group (EF) and intravenously parenteral nutrition group (PN). Equal volume of nutritional support fluid containing predetermined equal amount of calories and nitrogen was applied via feeding or intravenously infusion through external jugular vein. The indices were observed on 6, 12, 24, 48 and 72 postburn hours (PBHs) with the reference to those in 6 normal rats. The intestinal epithelial cell cycle in jejunal and ileal mucous membrane was analyzed by flow cytometry. Western blotting method was employed in the examination of the expression of cyclin D1, E and that of cyclin dependent kinase (CDK)2 and CDK4. RESULTS: (1) lntestinal mucosal epithelial G0/G1 ratio in jejunum in EF group was significantly lower than that in PN group at 72 PBHs (P < 0.05). While the ratio in ileum in EF was obviously higher than that in PN groups at 6, 12, 48 and 72 PBHs (P < 0.05). (2) The cell percentage of S phase in EF group was evidently higher than that in PN group (P < 0.05 - 0.01) at 48 and 72 PBHs. (3) Intestinal mucosal cyclin D1 expression increased significantly in EF group at 24 PBHs and in PN group at 48 PBHs (P < 0.05) and which in EF group was obviously higher than that in PN group at 72 PBHs (P < 0.05). (4) The expression of the intestinal mucosal cyclin E in EF group at 72 PBHs was evidently higher than the control value and that in PN group (P < 0.05). (5) The expression of CDK2 exhibited no obvious difference among PN,EF and control group (P < 0.05). The CDK4 expression in EF group increased obviously at 72 PBHs (P < 0.05). CONCLUSION: Early postburn enteral feeding was beneficial to the progression of intestinal mucosal epithelial cell cycle and to the repairing and renovation of injured intestinal mucosal membrane. Cyclin and CDK might be important in the modulation of the intestinal mucosal epithelial cell cycle.

Animals↗

PubMed2002

[An experimental study of the changes of rat hepatocytic glycolysis during hypoxia].

OBJECTIVE: To investigate the effects of hypoxia on the glycolysis in cultured rat hepatocytes. METHODS: Mixed gas with different concentrations of O(2), CO(2) and N(2) was prepared for the in vitro culture of normal rat hepatocytes. The cell strains were set to be A, B, C groups, which were observed at 1, 2, 4, 8 and 16 hours after hypoxia with normal hepatocytes as the control. Biochemical methods were employed to determine the activities of the key enzymes during hepatocytic glycolysis such as hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), lactate dehydrogenase (LDH) and the change of the content of lactic acid (LA) in the culture fluid. RESULTS: (1) The LDH activity of the rat hepatocytes increased significantly at all the time points of hypoxia in A and B groups when compared with that in control group (P < 0.05), while the activity increased obviously in C group since 2 hours after hypoxia (P < 0.05). (2) The HK activity of the cells in A group increased significantly at 1, 2, 4 and 16 hours after hypoxia and that in B and C groups increased obviously at 1 hour when compared with control group (P < 0.05). While the cellular PFK activity in A group increased markedly at 1 and 4 hours after hypoxia and that in B and C groups increased evidently at 4 hours after hypoxia (P < 0.05). The cellular PK activity in all the three groups increased at all the hypoxic time points (P < 0.05). (3) The cellular LA content in A and B groups began to increase since 2 hours and that in C group did so since 4 hours after hypoxia and increased along with the time lapse (P < 0.05). CONCLUSION: hypoxia might initiate glycolysis.

Animals↗

PubMed2002

[Survey of infection of Toxoplasma gondii in infertile couples in Suzhou countryside].

OBJECTIVES: To determine the level of anti-Toxoplasma antibody in serum of infertile couples to explore the relationship between toxoplasma infection and infertility. METHODS: Enzyme-linked immunosorbent assay (ELISA) was applied to detect the anti-Toxoplasma antibody, antisperm antibody (AsAb) and anticardiolipin antibody (ACA) in serum of 178 couples with infertility and 190 couples who had normal pregnant history. RESULTS: The positive result of Toxoplasma infection in the infertile couples was significantly higher than that in fertile couples which was 34.83% vs 12.11% (chi 2 = 26.72, P < 0.01) with the odds ratio 3.88. The positive result of serum AsAb in the Toxoplasma infected group was significantly higher than that in the no Toxoplasma infected group (32.50% vs 15.94%, chi 2 = 10.76, P < 0.01) with the odds ratio 2.54. CONCLUSIONS: Toxoplasma infection was related to infertility. The Toxoplasma infection and was posibly related to the antisperm antibodies which can be involved in the pathogenisis of infertility.

Animals↗

PubMedDate not supplied

Reduced synaptic vesicle density and active zone size in mice lacking amyloid precursor protein (APP) and APP-like protein 2.

Although abnormal processing of amyloid precursor protein (APP) leads to early onset of Alzheimer's disease, the normal function of this protein is poorly understood. APP is widely expressed in axons, dendrites, and synapses in both central and peripheral nervous systems. Mice homozygous for APP or its homologue APP-like protein 2 (APLP2) null mutation (KO) are viable, but double mutants for APP and APLP2 deletions (DKO) are early postnatal lethal. To investigate the role of APP in synapse development, we compared the ultrastructure of submandibular ganglion synapses between DKO and littermate APLP2 KO mice at birth. Using serial electron microscopy, we found that the size of presynaptic boutons and the number of active zones per bouton were comparable in both strains of animals. However, the synaptic vesicle density, active zone size, and docked vesicle number per active zone were significantly reduced in DKO compared to those in APLP2 KO. These results indicate that the APP family of proteins plays an important role in regulating the formation and function of inter-neuronal synapses.

Amyloid beta-Protein Precursor↗

PubMedDate not supplied

Near-field optical technique applied for investigation of the characteristics of polymer fiber and waveguide structures.

This article summarizes the near-field optical technique applied for investigating the characteristics of polymer fiber and waveguide structures. The near-field optical technique is used to analyze multimode interference structures of fiber. The localized fluctuation of the transmission caused by fractal cluster is carried out in Nd3+- and Eu3+-doped polymer fiber and film by means of a scanning near-field optical microscopy. The near-field optical spectrum of Nd3+-doped polymer fiber is investigated. The topography and near-field intensity images of Azo-polymer liquid crystal film for waveguide are obtained simultaneously.

Journal Article↗