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Pei-zeng Yang

Publications and source records attributed to Pei-zeng Yang.

11 recordsLinked to original sources
PubMed2006

Role of T-cell receptor V beta 8.3 peptide vaccine in the prevention of experimental autoimmune uveoretinitis.

BACKGROUND: T-cell receptor (TCR) plays an important role in the development of autoimmune diseases. Recently, it was reported that immunization of animals with TCR peptide derived from the pathogenic cells could prevent autoimmune diseases. The aim of this study was to investigate whether vaccination with a synthetic peptide from the hypervariable region of TCR V(beta) 8.3, an experimental autoimmune uveoretinitis (EAU)-associated gene, was able to prevent the disease. METHODS: EAU was induced in Lewis rats by immunization with IRBP R16 peptide emulsified in complete Freund's adjuvant (CFA). The clinical and histological appearances were scored. Delayed type hypersensitivity (DTH) and lymphocyte proliferation were detected. Cytokine levels of aqueous humour, supernatants of cells from spleen and draining lymph nodes were measured by enzyme linked immunosorbent assay (ELISA). Gene expression of TCR V(beta) 8.3 on CD(4)(+) T cells was examined by real time quantitative polymerase chain reaction (PCR). RESULTS: After vaccination, the intraocular inflammation was significantly mitigated, antigen specific DTH and lymphocyte proliferation responses were suppressed, interleukin (IL)-2 in aqueous humour, interferon (IFN)-gamma and IL-2 produced by the spleen and draining lymph node cells were significantly decreased, whereas the production of IL-4 and IL-10 were increased. The response of draining lymph node cells to TCR V(beta) 8.3 peptide was enhanced after vaccination. Inoculation with CFA alone did not affect the severity of EAU and the above parameters. The suppression of EAU was much stronger in the group of four fold inoculations than the group of two fold inoculations. The expression of TCR V(beta) 8.3 gene was significantly reduced in the group of fourfold inoculations. CONCLUSION: Vaccination with the synthetic TCR V(beta) 8.3 peptide could remarkably inhibit the development of EAU.

Animals↗
PubMed2006

[Effect of cyclosporin A on the production of IFN-gamma by murine splenocytes].

AIM: To explore the effect of cyclosporine A (CsA) on the production of IFN-gamma by murine splenocytes after stimulation with different reagents. METHODS: Mouse splenocytes were prepared and cultured with anti-CD3 mAb alone, anti-CD3 mAb plus anti-CD28 mAb or anti-CD3 mAb plus IL-12, in the presence or absence of different concentrations of CsA. After stimulating for 3 days, the content of INF-gamma in the culture supernatant was assayed by ELISA. The effects of CsA on the expression of activated molecules on T cells and intracellular IFN-gamma production were examined at the single cell level by flow cytometry. RESULTS: The mouse splenocytes stimulated with anti-CD3 mAb, anti-CD3 mAb + anti-CD28 mAb or anti-CD3 mAb + IL-12 could produce high level of IFN-gamma, but CsA inhibited INF-gamma production in a dose-dependent manner. The inhibitory mechanism of CsA was related to the activation of splenocytes. CONCLUSION: CsA can inhibit the expressions of CD25 and CD69 on splenocytes, as well as INF-gamma production by splenocytes in a dose dependent manner.

Animals↗
PubMed2005

GATA-3 expression in the development of anterior chamber associated immune deviation.

BACKGROUND: Anterior chamber associated immune deviation (ACAID) is characterized by a Th2 cell response. GATA-3 has been shown to be necessary for the activation of Th2 cells. This study was designed to examine the expression of GATA-3 in the development of ACAID. METHODS: ACAID was induced by injection of 50 microg interphotoreceptor retinoid binding protein (IRBP) into the anterior chamber (AC) of Wistar rats. Delayed-type hypersensitivity (DTH) was evaluated on day 3, 7, 14, 21, 28 after IRBP inoculation. GATA-3 expression was detected using immunohistochemical staining. The expression of GATA-3 mRNA at different time points after AC injection of IRBP was assayed by reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: A significant DTH reaction was observed in Wistar rats on day 3 and 5 after IRBP inoculation. The DTH reaction was decreased 7 days after IRBP inoculation. GATA-3 expression was weak at both mRNA and protein levels in the normal spleen, but was significantly increased on day 5, 7, 14, and 21 after AC injection of IRBP. CONCLUSION: The expression of GATA-3 is increased during ACAID, suggesting that GATA-3 may be involved in the development of ACAID.

Animals↗
PubMed2005

[Clinical diagnosis and treatment of uveitis associated with ankylosing spondylitis].

OBJECTIVE: To investigate the clinical features, diagnosis, treatment and prognosis of uveitis associated with ankylosing spondylitis (AS). METHODS: A history about low back pain was carefully reviewed in patients with uveitis and X-ray examination was performed if necessary. Forty-four patients were diagnosed as AS from January 1996 to June 2001 in Zhongshan Ophthalmic Center according to the modified New York criteria for AS. Data concerning these patients, especially with respect to the clinical features, diagnosis and treatment, were retrospectively analyzed. RESULTS: Of 44 patients, 41 were male. The age of the patients at onset of uveitis was (33 +/- 15) years old. All of the 34 patients who had a uveitis history over 40 days showed a recurrent inflammation. In 18 patients (52.9%), the interval between the relapse of uveitis was more than one year. Bilateral ocular involvement was found in 23 out of 34 patients with recurrent uveitis. However, none of them showed a bilateral inflammation at the onset of uveitis. All patients showed acute anterior uveitis with a duration of (27 +/- 12) days. All patients had definitely radiological evidences of bilateral sacroiliitis, although not all of them had typical history of lower back pain. Treatment with corticosteroids eyedrops and cycloplegic agent was used in all patients. Vision equal to or better than 1.0 was achieved in 82.5% of these patients. CONCLUSIONS: Uveitis associated with AS is characterized by acute nongranulamatous anterior uveitis with recurrent episodes in male. Diagnosis is made according to typical clinical features and radiological evidences of bilateral sacroiliitis. Treatment with corticosteroid eyedrops and cycloplegic is able to resolve the inflammation rapidly and leads to a good prognosis in most patients.

Adolescent↗
PubMed2005

[Recent advances in clinical and basic studies of uveitis in China in the last five years].

Uveitis is one of the most important causes of blindness throughout the world. Its etiology and pathogenesis are rather complicated and have not been well understood. Chinese ophthalmologists have paid much attention to the studies on the pathogenesis, treatment and prevention of uveitis and great achievement has been obtained during recent years. However, there are still misunderstandings and undue treatment in the field of uveitis. A large number of uveitis entities are inappropriately or simply diagnosed as uveitis, thereby leading to a uniform treatment for these patients. Furthermore, abuse of corticosteroids is still rather common. Therefore, it is necessary to popularize the modern knowledge of uveitis among Chinese ophthalmologists and to improve basic and clinical uveitis studies in China.

China↗
PubMed2005

[Advances in uveitis study].

Uveitis is one of the important causes of blindness in the world. Uveitis has been generally defined as the inflammation occurring in the intraocular tissues. The causes and entities of uveitis have changed over time. The mechanism for the pathogenesis of uveitis has been extensively studied and great achievement has been obtained during recent years. Novel techniques for uveitis diagnosis and the application of immunoregulatory and biologic agents have improved the treatment for the uveitis patients.

Humans↗
PubMed2004

[Increased expression of Fas and FasL mRNA in peripheral blood lymphocytes in Vogt-Koyanagi-Harada syndrome].

OBJECTIVE: To evaluate the expression of Fas and FasL messenger RNA (mRNA) in peripheral blood lymphocytes and its possible role in the pathogenesis of Vogt-Koyanagi-Harada syndrome. METHODS: Blood samples were obtained from 16 patients with Vogt-Koyanagi-Harada syndrome and 19 healthy individuals from January to June, 2000. Total RNA was extracted from peripheral blood lymphocytes. cDNA was prepared from RNA with oligo d(T)(18) and M-MuLV reverse transcriptase. The expression of Fas mRNA and FasL mRNA in peripheral blood lymphocytes was determined using fluorescent quantitative polymerase chain reaction (FQ-PCR). Amplification was carried out through 32 cycles, 30 seconds denaturation at 93 degrees C, 30 seconds annealing at 58 degrees C, and 45 seconds primer extension at 72 degrees C. The final extension time was 7 minutes. RESULTS: The expression of Fas mRNA on peripheral blood lymphocytes [(1.6 +/- 2.0) x 10(6)] was significantly higher than that of the controls [(5.7 +/- 2.0) x 10(5)] (t = 4.50, P < 0.05). The mRNA expression of FasL on peripheral blood lymphocytes [(1.8 +/- 1.5) x 10(6)] was also higher than that of the controls [(4.8 +/- 3.5) x 10(5)] (t = 9.57, P < 0.05). CONCLUSIONS: A high level of Fas mRNA and FasL mRNA expression in activated peripheral blood lymphocytes is observed in patients with Vogt-Koyanagi-Harada syndrome. These long-persisted activated lymphocytes may be responsible for the perpetuation and recurrence of Vogt-Koyanagi-Harada syndrome.

Adult↗
PubMed2004

[Quantitative determination of aqueous flare and cells in the eyes of patients with inflammation of anterior uvea].

OBJECTIVE: To quantify aqueous flare and cells in the eyes of patients with inflammation of anterior uvea by FC-2000 laser flare cell meter (LFCM), and to compare these results with those obtained with slit lamp microscopy. METHODS: Aqueous flare and cells of 194 eyes of 110 patients with inflammation of anterior uvea and 52 eyes of 52 healthy subjects were graded into 0, 1+, 2+, 3+ and 4 + scale based on a previously described system using slit lamp microscopy. LFCM was also used for evaluation of aqueous flare and cells. RESULTS: All eyes in normal individuals were graded as "0" scale of both flare and cells by silt lamp microscopy. Flare of grade 0, 1+ and 2+ were noted in 74, 98 and 18 eyes, and cell of grade 0, 1+, 2+, 3+ and 4+ were noted in 124, 26, 19, 14 and 11 eyes in uveitis patients, respectively. LFCM examination revealed that the mean flare values in uveitis eyes with flare of grade 0, 1+ and 2+ were 7.9, 29.5 and 189.0 photon count/ms, respectively. In patients with flare of grade 3+ and 4+, LFCM readings were unreliable because of increased background noise. There was significant correlation between slit lamp examination and the laser flare measurement for flare of grade 0, 1+ and 2+ (r = 0.75, P < 0.001). The mean flare values were significantly higher in patients with flare of grade 0, 1+ and 2+ than that in normal controls (5.3 pc/ms) (t = 5.872, P < 0.05). The mean cell numbers in the eyes with cell of grade 0, 1+, 2+, 3+ and 4+ were 1.5, 12.1, 33.9, 84.9 and 193.1 count/0.5 mm(3), respectively. The results of slit lamp examination showed significant correlation with laser cell counts measurement (r = 0.72, P < 0.001). The mean cell numbers were significantly higher in uveitis patients than that in normal controls (0.9 count/0.5 mm(3)) (t = 7.351, P < 0.05). CONCLUSIONS: Our results indicate that LFCM is able to evaluate precisely the mild and moderate breakdown of blood aqueous barrier and inflammation of the anterior uvea tract, therefore it provides an important parameter for the treatment of anterior uveitis.

Adolescent↗
PubMed2004

[Evaluation of the problems in the treatment of uveitis].

OBJECTIVE: To address the problems present in the treatment of uveitis. METHODS: A retrospective analysis was made on 154 patients (268 eyes) with different entities of uveitis, coming from all over China and referring to Zhongshan ophthalmic center from April to October of 2000. The data of these patients, especially with respect to the usage of corticosteroids and antibiotics in the referred hospitals, were analyzed. RESULTS: Corticosteroids drops, subconjunctival or retrobulbar injection and systemic corticosteroids were respectively used in 90.3% and 84.0% of these eyes, and 92.9% of these cases. Intravenous injection of corticosteroids was rather popular, accounting for 85.3% of the cases who were treated with systemic corticosteroids. Improperly aggressive treatment with corticosteroids or undue application of their eye drops was equally found in patients from county-, city- and province-level hospitals, especially in Behcet's disease, anterior uveitis and Fuchs heterochromic uveitis. Antibiotics drops, subconjunctival injection and systemic administration of antibiotics were respectively used in 97.0% and 71.6% of these eyes and 83.8% of these patients. Immunosuppressive drugs, other than the corticosteroids, were used in 6 patients only. CONCLUSIONS: Corticosteroids should be used in proper methods, dosage and duration based on the entities and severity of uveitis. It is not necessary to treat noninfectious uveitis with antibiotics.

Adolescent↗
PubMed2003

[Effect of endotoxin-induced uveitis on GATA-3 expression and ACAID development].

OBJECTIVE: To investigate the changes of the GATA-3 expression and development of anterior chamber associated immune deviation (ACAID) after anterior chamber (AC) injection of interphotoreceptor retinoid binding protein (IRBP) under ocular inflammation. METHODS: ACAID was induced by injection of IRBP into the AC of 30 Spar-Dawley (SD) rats. Then the animals were divided into -4 days group, -24 hours group, 0 hour group, 3 days group, and 7 days group according to the lipopolysaccharide (LPS) injection 4 days and 24 hours before, or 0 hour, 3 days and 7 days after IRBP inoculation respectively. 6 rats were used as controls 8 days after IRBP injection, Serum interleukin-4 (IL-4) was evaluated to count the development of ACAID; Western blot and RT-PCR were used to determine the protein and mRNA levels of GATA-3 expression. RESULTS: In -24 hours group and 0 hour group, the ocular inflammation reached a maximum 24 hours after LPS injection; on 8 days after IRBP inoculation, serum IL-4 couldn't be detected and GATA-3 expression were not changed both on mRNA and protein levels compared with control group. In -4 days group, the ocular inflammation was subsided gradually 24 hours and disappeared 96 hours after LPS injection; serum IL-4 and GATA-3 expression were significantly elevated 8 days after IRBP injection. In 7 days group, the serum IL-4 and GATA-3 expression in spleen increased 8 days after IRBP inoculation. CONCLUSION: In ocular inflammation, the up-regulation of GATA-3 expression is inhibited and ACAID development is blocked after antigen was injected into anterior chamber. Once GATA-3 is up-regulated, LPS injection cannot affect ACAID development.

Animals↗