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Peter Imle

Publications and source records attributed to Peter Imle.

2 recordsLinked to original sources

Aspects influencing genotyping method selection.

The variety of genotyping methods currently available and the evolution of their capabilities have facilitated an expansion of the field of pharmacogenomics. Traditionally, limited genotyping capabilities have restricted the generation and application of genotyping data for pharmacogenomic studies. With the variety of platforms and chemistries available for flexible, high-throughput genotyping, it is important to keep in mind the limitations imposed by both the polymorphisms that are to be interrogated and the type of pharmacogenomics study for which the data are being generated. This chapter is an overview of the constraints these factors impose on different genotyping methods and describes aspects important to the integration of genotyping into a pharmacogenomics study.

Alleles↗

Fluorescence-based fragment size analysis.

The successful application of capillary electrophoresis technology to the genotyping of various types of polymorphisms has been well documented. The flexibility and automation of the Applied Biosystems 3100 Genetic Analyzer make it an excellent capillary electrophoresis platform for the generation of high quality genotype data. These data are readily applied to pharmacogenomic investigations of various types. Included in this chapter is a protocol for the generation of genotype data using minimal template deoxyribonucleic acid and maximizing the automation of both data analysis and genotype assignment through the use of the Applied Biosystems GeneMapper 3.0 software package.

DNA↗