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Peter Kämpfer

Publications and source records attributed to Peter Kämpfer.

At least 73 records · Page 4Linked to original sources
PubMed2003

Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval 'Virgilkapelle' in Vienna.

Two airborne bacterial isolates designated V45(T) and V54A(T) were characterized in order to determine their taxonomic position. 16S rDNA sequence analysis showed that the two isolates shared 98.1 % sequence similarity. Highest sequence similarities (98.0-98.5 %) were found to Promicromonospora citrea DSM 43110(T) and Promicromonospora sukumoe IFO 14650(T). Detection of a quinone system with the predominant compound MK-9(H(4)), a polar lipid pattern containing phosphatidylglycerol, a fatty acid profile with the predominant acids C(15 : 0) iso and C(15 : 0) anteiso and the diagnostic cell-wall diamino acid L-lysine supported the assignment of the novel isolates to the genus PROMICROMONOSPORA: The two isolates could be distinguished from P. sukumoe by the presence of glycine in the peptidoglycan, and the detection of the cell-wall sugar galactose differentiates them from the two established species of the genus PROMICROMONOSPORA: Each of the two isolates displayed a unique biochemical profile. Results from DNA-DNA hybridizations clearly demonstrated that V45(T) and V54A(T) represent separate species. Based on these data, it is proposed that V45(T) (=IFO 16525(T)=CCM 7044(T)) and V54A(T) (=IFO 16526(T) =CCM 7043(T)) be classified as the type strains of two novel Promicromonospora species, for which the names Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov. are proposed.

Actinobacteria↗
PubMed2003

Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov.

A format for the description of single novel species is proposed, which should facilitate the reviewing process by assisting the provision of data in a standardized form. The abstract must be short and concise, highlighting phylogenetic position, morphology and chemotaxonomy for genus affiliation, the genotypic and phenotypic basis for species differentiation, and the name and deposition numbers from two public culture collections in different countries for the type strain: A Gram-negative, rod-shaped, non-spore-forming bacterium (Iso 196(T)) was isolated from chicken faeces. On the basis of 16S rRNA gene sequence similarity, strain Iso 196(T) was shown to belong to the alpha-2 subclass of the Proteobacteria related to Ochrobactrum tritici (95.6%), Ochrobactrum grignonense (95.0%) and Ochrobactrum anthropi (94.6%), and the phylogenetic distance from any validly described species within the genus Brucella was less than 95%. Chemotaxonomic data (major ubiquinone - Q-10; major polyamines - spermidine and putrescine; major polar lipids - phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine; major fatty acids - C(18 : 1)omega7c and C(19 : 0) cyclo omega8c) supported the affiliation of strain Iso 196(T) to the genus Ochrobactrum. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Iso 196(T) from the four validly published Ochrobactrum species. Iso 196(T) therefore represents a new species, for which the name Ochrobactrum gallinifaecis sp. nov. is proposed, with the type strain Iso 196(T) (= DSM 15295(T) = CIP 107753(T)).

Bacterial Typing Techniques↗
PubMed2002

Novosphingobium hassiacum sp. nov., a new species isolated from an aerated sewage pond.

The taxonomy of two strains W-51T and W-52 isolated from a wastewater treatment plant was investigated in a polyphasic approach. The yellow pigmented gram-negative organism contained a quinone system with mainly ubiquinone Q-10, and the polar lipid profile contained a sphingoglycolipid suggesting that both strains belonged to the the alpha-4 subclass of the Proteobacteria. The polar lipid profile consisted furthermore of phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylcholine and of minor amounts of phosphatidylglycerol and phosphatidylmonomethylethanolamine. Sequencing of the 16S rRNA gene supported the allocation into the genus Novosphingobium, together with the type strains of N. subterraneum, N. aromaticivorans, N. stygium, and N. capsulatum, showing similarities of 97.3%, 97.0%, 95.7% and 96.2%, respectively. This allocation was supported by the polyamine profile, which consisted mainly of spermidine. The analysis of the fatty acids revealed 2-OH 13:0, 2-OH 14:0 and 2-OH 15:0, with 2-OH 15:0 as predominant hydroxylated fatty acid. W-51T and W-52 were almost identical with respect to their phenotypic including the majority of the chemotaxonomic properties, identical in their 16S rRNA sequences, and showed 86% DNA-DNA similarity. Both strains were able to reduce nitrate and on the basis of further physiological features, a clear differentiation from all other Novosphingobium species was possible. The DNA-DNA similarities of W-51T to the type strains of N. subterraneum, N. aromaticivorans, and N. capsulatum were below 56%. For these reasons, it is proposed to create a new species with the name Novosphingobium hassiacum sp. nov.

Aerobiosis↗
PubMed2002

Psychrobacter faecalis sp. nov., a new species from a bioaerosol originating from pigeon faeces.

The taxonomy of strain Iso-46T isolated from a bioaerosol generated by cleaning of a pigeon faeces contaminated room was investigated in a polyphasic approach. The beige pigmented Gram-negative, oxidase-negative organism contained a quinone system with mainly ubiquinone Q-8, and the polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, beside some hitherto uncharacterized phospholipids. Major polyamines were spermidine and putrescine and also small amounts of cadaverine. The analysis of the fatty acids revealed 3-OH 12:0 and 3-OH 14:0 (within summed feature 3) as hydroxylated fatty acids. These chemotaxonomic characteristics suggest that the strain belongs to the gamma-subclass of the Proteobacteria namely into the genus Psychrobacter. Analysis of the 16S rRNA gene supported the allocation into the genus Psychrobacter, but showing similarities to all described species of this genus lower than 97%. Iso-46T was able to grow on MacConkey agar and other high nutrient containing media within a temperature range of 4 degrees C to 36 degrees C. On the basis of nutritional and further physiological features, a clear differentiation from all other Psychrobacter species was possible. For these reasons it is proposed to create a new species with the name Psychrobacter faecalis sp. nov.

Aerosols↗
PubMed2002

Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov.

Three aerobic, gram-negative, rod-shaped, non-spore-forming, red-pigmented, airborne bacteria (I/26-Cor1T, I/32A-Cor1 and I/74-Cor2) collected in the Museo Correr (Venice, Italy) were investigated to determine their taxonomic status by analysing their biochemical, physiological and chemotaxonomic features and the G+C content of genomic DNA and by comparing their genomic fingerprints. Additionally, the almost complete 16S rRNA gene sequence of strain I/26-Cor1T was analysed. The three strains were nearly identical in their morphological, physiological, biochemical and chemotaxonomic properties. The strains contained a menaquinone system with the predominant menaquinone MK-7 and a fatty acid profile with C15:0 anteiso, C15:0 iso and C16:1 predominant. Phosphatidylethanolamine and several unidentified lipids were detected in the polar lipid profiles. The polyamine pattern consisted of sym-homospermidine as the major compound. meso-Diaminopimelic acid was found as the characteristic cell-wall diamino acid. The DNA base composition of the three strains ranged from 60 to 63 mol% G+C. Phylogenetically, strain I/26-Cor1T was most closely related to Hymenobacter actinosclerus (95.8% 16S rRNA gene sequence similarity). Physiological and genomic characteristics indicated that the two strains I/26-Cor1T and I/32A-Cor1 are representatives of the same species. The phylogenetic distance to any validly described taxon as indicated by 16S rRNA gene sequence similarities demonstrates that I/26-Cor1T and I/32A-Cor1 represent a novel species, for which the name Hymenobacter aerophilus sp. nov. is proposed, with the type strain I/26-Cor1T (= DSM 13606T = LMG 19657T). I/32A-Cor1 (= DSM 13607 = LMG 19658) is another strain of the species Hymenobacter aerophilus. Since the taxonomic status of strain I/74-Cor2 within the genus Hymenobacter was not determined unambiguously, it is designated Hymenobacter sp. I/74-Cor2 (= DSM 13611 = LMG 19659).

Air Microbiology↗
PubMed2002

Nocardiopsis compostus sp. nov., from the atmosphere of a composting facility.

Three strains (KS8, KS9T and KS21), isolated from air samples near a composting facility, were subjected to taxonomic analyses (characterized using a polyphasic approach). Morphological and chemotaxonomic characteristics of the isolates were in agreement with those described for members of the genus Nocardiopsis. On the basis of 16S rRNA sequence comparison and phenotypic tests, KS21 clearly belonged to Nocardiopsis alba. KS8 and KS9T showed less than 98% 16S rRNA gene sequence similarity to any of the previously described Nocardiopsis species. The polar lipid profiles of both isolates consisted of four major compounds, phosphatidylmonomethylethanolamine, phosphatidylcholine, diphosphatidylglycerol and phosphatidylglycerol, in addition to two unknown phospholipids. The major menaquinones in KS8 and KS9T were MK-10(H8), MK-11(H8), MK-10(H6) and MK-12. Furthermore, MK-13, MK-11(H6), MK-9(H8) and MK-10(H4) could be detected in significant amounts. The fatty acid composition included iso- and anteiso-branched acids combined with tuberculostearic acid (Me18:0), straight-chain saturated (16:0, 18:0) and unsaturated (16:1, 17:1, 18:1) fatty acids. On the basis of these results, KS8 and KS9T clearly represent a novel species of the genus Nocardiopsis, for which the name Nocardiopsis compostus sp. nov. is proposed (type strain KS9T = DSM 44551T= NRRL B-24145T).

Actinomycetales↗
PubMed2002

Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974).

Nine yellow-pigmented, spherical bacterial strains isolated from a medieval wall painting (strain D7), from indoor air (strains 3, 6, 7, 13C2, 38, 83 and 118) and from an activated-sludge plant (strain Ballarat) were classified by a polyphasic approach. Analyses of the 16S rRNA gene sequences of three representatives (strains D7, 118 and Ballarat) indicated that they all belong to the genus Micrococcus. The three isolates shared the highest sequence similarities with Micrococcus luteus DSM 20030T (97.9-98%), Micrococcus antarcticus AS 1.2372T (97.9-98.3%) and Micrococcus lylae DSM 20315T (97.5-97.9%). DNA-DNA reassociation studies clearly demonstrated that all nine isolates belong to the species M. luteus. However, neither their chemotaxonomic features nor their physiological and biochemical properties were consistent with those of M. luteus DSM 20030T. In contrast to M. luteus DSM 20030T, all isolates investigated possessed MK-8(H2) as the major respiratory quinone, and strain Ballarat had an A4alpha peptidoglycan type. On the basis of analyses of their Fourier transform-infrared spectroscopy spectra, isolates D7, 3, 6, 7, 13C2, 38, 83 and 118 could be grouped into a single cluster separate from M. luteus DSM 20030T, strain Ballarat and M. lylae DSM 20315T. In addition, all these isolates could be distinguished from M. luteus DSM 20030T by their ability to assimilate D-maltose, D-trehalose, DL-3-hydroxybutyrate, DL-lactate, pyruvate and L-histidine and to hydrolyse casein. Strains D7, 3, 6, 7, 13C2, 38, 83 and 118 differed from both M. luteus DSM 20030T and strain Ballarat by their ability to assimilate acetate, L-phenylalanine, L-serine and phenylacetate. Furthermore, REP-PCR fingerprinting yielded one common band for these strains, whereas this band was not observed for M. luteus DSM 20030T, strain Ballarat or M. lylae DSM 20315T. On the basis of these data, the species M. luteus can be divided into three biovars that are distinguished by several chemotaxonomic and biochemical traits: biovar I, represented by M. luteus DSM 20030T; biovar II, represented by strains D7 (= DSM 14234 = CCM 4959), 3, 6, 7, 13C2, 38, 83 and 118; and biovar III, represented by strain Ballarat (= DSM 14235 = CCM 4960). On the basis of the results generated in this study, emended descriptions of the genus Micrococcus and the species M. luteus and M. lylae are given.

Air Microbiology↗
PubMed2002

Aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children with diarrhoea in Bangladesh, and extended description of Aeromonas hydrophila subsp. hydrophila (Chester 1901) Stanier 1943 (approved lists 1980).

A group of 10 Aeromonas hydrophila-like strains referred to as group BD-2, which mainly comprised haemolytic and cytotoxic isolates from diarrhoeal children in Bangladesh, was subjected to a polyphasic taxonomic study including fluorescent amplified fragment length polymorphism (FAFLP) and ERIC-PCR fingerprinting, microplate DNA-DNA hybridization and extensive phenotypic characterization. Comparison of FAFLP and ERIC-PCR genotypic profiles indicated that the group BD-2 isolates constituted a separate taxon that was most closely related to A. hydrophila DNA hybridization group (HG) 1. Determination of DNA-DNA hybridization values with type and reference strains of all known Aeromonas taxa revealed that the BD-2 group represented a homogeneous taxon (internal DNA homology, 77-94%) that exhibited 78-92% DNA relatedness with the type strain of A. hydrophila HG1. These results indicated that the BD-2 group belongs genotypically to A. hydrophila HG1, but examination of 152 phenotypic characteristics revealed that the group BD-2 isolates differed from representatives of HG1 in eight biochemical properties. The diagnostic value of at least three of these tests, namely utilization of urocanic acid and L-arabinose and acid production from L-arabinose, has been confirmed outside this study by other workers. Collectively, the genotypic and phenotypic data reported suggest that the BD-2 group represents a subspecies of A. hydrophila, for which the name Aeromonas hydrophila subsp. dhakensis subsp. nov. is proposed. Its type strain is P21T (= LMG 19562T= CCUG 45377T). An extended description of A. hydrophila subsp. hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980) is given.

Aeromonas hydrophila↗
PubMed2002

Georgenia muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting.

Two bacterial strains, designated 1A-C(T) and 3A-1, were studied and, using these results and previously published data, taxonomically classified. Cells of the strains exhibited a rod-coccus cycle. The peptidoglycan determined for 1A-C(T) was of type A4alpha with lysine as the diagnostic cell-wall diamino acid and an interpeptide bridge of L-Lys <-- L-Glu. The menaquinone systems of the two strains contained MK-8(H4) (82-94%) and MK-7(H4) (3-11%). The polar lipid profiles consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, two unidentified phospholipids and an unidentified glycolipid. The fatty acid profiles contained predominantly ai-C15:0 and significant amounts of i-C14:0 and i-C15:1 fatty acids. Genomic fingerprints clearly distinguished strains 1A-C(T) and 3A-1 from each other. DNA-DNA relatedness between the two strains (92%) demonstrated that they are members of a single species. Analyses of the 16S rDNA sequences of strains IA-C(T) and 3A-1, which were almost identical (99.6% sequence similarity), and comparison with corresponding sequences demonstrated that they represent a novel lineage within the suborder Micrococcineae, most closely related to species of the genera Beutenbergia, Bogoriella and Cellulomonas (94.7-95.7% sequence similarity). The results demonstrate that the two strains are members of a single new genus and a single novel species. Thus, the name Georgenia muralis gen. nov., sp. nov. is proposed. The type strain is strain 1A-C(T) (= DSM 14418T = CCM 4963T). Another strain of the species is strain 3A-1 (= DSM 14419 = CCM 4964).

Actinomycetales↗
PubMed2002

Cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis.

A novel cellulolytic and xylanolytic bacterium, strain MX5T, was isolated from the hindgut contents of the Australian termite Mastotermes darwiniensis (Froggatt). The isolate was a facultative anaerobe and had a Gram-positive cell-wall profile. The rod-shaped bacterium formed irregular coryneform and coccoid cells during growth. Phylogenetic analysis of the 16S rDNA provided evidence that the organism was closely related to the as-yet undescribed cellulolytic strain SR272 and the non-validly described species 'Cellulomonas pachnodae' as well as Promicromonospora citrea and Promicromonospora sukumoe. Strain MX5T was assigned to the genus Cellulosimicrobium on the basis of phylogenetic and chemotaxonomic criteria. The murein of strain MX5T contained the diamino acid lysine. N-Glycolylmuramic acid, mycolic acids and hydroxy fatty acids were absent. The major neutral sugar in the cell wall was galactose and the major quinone was menaquinone MK-9(H4). The predominant fatty acids were ai-C15:0, i-C15:0, i-C16:0 and C16:0. The G+C content of the DNA was in a range 70-72 mol%. On the basis of 16S rDNA sequence similarities and chemotaxonomic features, MX5T was clearly different from Cellulosimicrobium cellulans and other validly described species within this phylogenetic group. For this reason, a novel species is described, for which the name Cellulosimicrobium variabile sp. nov. is proposed.

Actinomycetales↗
PubMed2002

Agrococcus baldri sp. nov., isolated from the air in the 'Virgilkapelle' in Vienna.

Five coccoid, Gram-positive strains were isolated from the air of the 'Virgilkapelle' in Vienna. A representative of these five strains, V-108T, shared 99.0 and 98.4% 16S rDNA sequence similarity, respectively, with Agrococcus jenensis DSM 9580 and Agrococcus citreus DSM 12453T. Colonies of the five strains were white when grown in the dark and turned yellow in the light. The strains displayed highly similar biochemical and physiological characteristics and showed only small differences in their protein patterns obtained after SDS-PAGE. Based on Fourier-transform infra-red (FT-IR) spectra, the five strains were grouped together and separated from the other members of the genus, A. jenensis and A. citreus. Chemotaxonomic characteristics analysed from selected members of the five isolates, including polar lipids, quinone systems, polyamine patterns, cell wall composition and fatty acid profiles, were in good agreement with those of the two species of the genus Agrococcus described to date. The G+C content of the genomic DNA was determined to be within the narrow range of 73.8-74.9 mol%. The results of DNA-DNA hybridization with A. citreus DSM 12453T and A. jenensis DSM 9580T, as well as differences in biochemical/physiological characteristics, peptidoglycan composition, fatty acids, polar lipid profiles and FT-IR spectra, demonstrated that the five isolates represent a novel species of the genus Agrococcus. The name Agrococcus baldri sp. nov. is proposed for the novel species, of which strain V-108T (= DSM 14215T = CCM 4953T) is the type strain.

Actinomycetales↗
PubMed2002

Microbacterium aerolatum sp. nov., isolated from the air in the 'Virgilkapelle' in Vienna.

Three rod-shaped, Gram-positive strains were isolated from the air of the chapel 'Virgilkapelle' in Vienna. A representative of these three strains, strain V-73T, shared the highest 16S rDNA sequence similarities with members of the genus Microbacterium, in particular Microbacterium foliorum, Microbacterium testaceum, Microbacterium esteraromaticum, Microbacterium keratanolyticum and Microbacterium arabinogalactanolyticum. The strains displayed almost identical biochemical and physiological characteristics and showed no differences in their protein patterns obtained after SDS-PAGE. On the basis of Fourier-transform infra-red (FT-IR) spectra and genomic fingerprints, the three strains were grouped together and separated from the other relevant members of the genus Microbacterium. The chemotaxonomic characteristics analysed, including polar lipids, quinone systems, cell wall composition and fatty acid profiles, were in good agreement with the characteristics described for the genus Microbacterium. The G+C content of the DNAs was determined to be in the narrow range 69.3-69.7 mol %. The results of DNA-DNA hybridization, biochemical/physiological characterization, ERIC-PCR-generated genomic fingerprints and FT-IR spectra demonstrated that the three isolates represent a novel species of the genus Microbacterium. The name Microbacterium aerolatum sp. nov. is proposed for the novel species, of which strain V-73T (= DSM 14217T = CCM 4955T) is the type strain.

Actinomycetales↗
PubMed2002

Erythrobacter citreus sp. nov., a yellow-pigmented bacterium that lacks bacteriochlorophyll a, isolated from the western Mediterranean Sea.

Two facultatively oligotrophic, intensely yellow-pigmented bacterial strains, RE35F/1T and RE10F/45, have been previously isolated from the western Mediterranean Sea (Bay of Calvi, Corsica, France) by 0.2 microm membrane filtration. The organisms were gram-negative, catalase- and oxidase-positive, strictly aerobic, rod-shaped and non-motile. Their respiratory lipoquinone profiles consisted exclusively of ubiquinone-10 (Q-10) and the G+C contents of their DNAs were 62.0 and 62.4 mol%, respectively. Among the cellular fatty acids, octadecenoic acid (18:1omega7c) was the major component. Both isolates also contained hydroxy fatty acids (14:0 2-OH, 18:1 2-OH and 16:0 iso 3-OH) and branched fatty acids (15:0 anteiso, 16:0 anteiso and 17:0 anteiso). Polar lipid fingerprints were characterized by the presence of a sphingoglycolipid. Comparative analyses of their 16S rRNA gene sequences indicated that both isolates were phylogenetically closely related (sequence similarity of 99.9%) and formed a coherent cluster with aerobic bacteriochlorophyll a-containing species of the Erythrobacter/Porphyrobacter/Erythromicrobium cluster within the family Sphingomonadaceae. The closest relative was Erythrobacter litoralis DSM 8509T (97.4 and 97.5% 16S rRNA gene sequence similarity between this strain and RE35F/1T and RE10F/45, respectively). DNA-DNA reassociation studies confirmed that strains RE35F/1T and RE10F/45 represent a single species (79.6% DNA homology), but also demonstrated that they do not belong to the species Erythrobacter litoralis (25.2 and 34.2% DNA homology, respectively). Notably, both RE35F/1T and RE10F/45 lacked bacteriochlorophyll a. Based upon phenotypic and molecular evidence, a novel species of the genus Erythrobacter, Erythrobacter citreus sp. nov., is proposed. Strain RE35F/1T (= CIP 107092T = DSM 14432T) is the type strain.

Alphaproteobacteria↗
PubMed2002

Sphingopyxis witflariensis sp. nov., isolated from activated sludge.

Classification of strain W-50(T), which was isolated from a wastewater treatment plant, was investigated by a polyphasic approach. Cells of strain W-50(T) were Gram-negative, strictly aerobic, oxidase-positive and yellow-pigmented. Ubiquinone Q-10 was the main respiratory lipoquinone system and polar lipid fingerprints were characterized by the presence of a sphingoglycolipid, suggesting that strain W-50(T) belongs to the alpha-4 subclass of the Proteobacteria. Sequencing and comparative analyses of the 16S rRNA gene of strain W-50(T) supported its chemotaxonomic allocation as an alpha-4 proteobacterium. The most closely related established taxa were species of the genus Sphingopyxis, including Sphingopyxis macrogoltabida (97.3% similarity) and Sphingopyxis terrae (96-4% similarity), and Sphingomonas taejonensis (97.3%). These findings were supported by both the polyamine content, which consisted mainly of spermidine [12.9 micromol (g dry wt)(-1)], and the presence of 2-OH 14:0, 2-OH 15:0 and 2-OH 16:0 in the cellular fatty acid profile. DNA-DNA hybridization experiments resulted in similarity values of 31.9% between strain W-50(T) and Sphingopyxis macrogoltabida IFO 15033(T), 44.9% between strain W-50(T) and Sphingopyxis terrae IFO 15098(T) and 31.0% between strain W-50(T) and Sphingomonas taejonensis KCTC 2884(T). Based upon results obtained by detailed physiological/biochemical testing and previously published molecular evidence, strain W-50(T) was clearly distinguishable from all other Sphingopyxis species. For these reasons, the creation of a novel species, Sphingopyxis witflariensis sp. nov., is proposed; strain W-50(T) (= DSM 14551(T) = CIP 107174(T)) is the type strain.

Alphaproteobacteria↗
PubMed2002

Citricoccus muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting.

A Gram-positive, aerobic, spherical actinobacterium, designated strain 4-0(T), was isolated from a medieval wall painting and characterized to determine its taxonomic position. The peptidoglycan of strain 4-0(T) was of type A4alpha, with lysine as the diagnostic cell wall diamino acid and an interpeptide bridge of Lys-Gly-Glu. Its quinone system contained predominantly MK-9(H2) (64%) and its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, four unknown glycolipids, two unknown phospholipids and an unknown lipid. The fatty acid profile of strain 4-0(T) was represented by significant amounts of ai-C15:0 and moderate amounts of ai-C17:0, i-C16:0 and i-C15:0 fatty acids. Spermidine was predominant in the polyamine pattern. The G+C content of the genomic DNA was 68 mol%. Comparative 16S rDNA sequence studies revealed the highest similarity values (95.4-96.1%) between strain 4-0(T) and species of the genus Micrococcus and certain species of the genus Arthrobacter, including Arthrobacter pascens DSM 20545(T), Arthrobacter ramosus DSM 20546(T), Arthrobacterprotophormiae DSM 20168(T), Arthrobacternicotianae DSM 20123(T) and Arthrobacter globiformis DSM 20124(T). Phylogenetic analyses demonstrated that strain 4-0(T) branches deeply on the Micrococcus lineage. Because it is almost equidistant phylogenetically from the genera Micrococcus and Arthrobacter and possesses significant differences in chemotaxonomic characteristics from members of these genera, it is suggested that strain 4-0(T) be classified as a novel species in a new genus; the name Citricoccus muralis gen. nov., sp. nov. is proposed. The type strain is strain 4-0(T) (= DSM 11442(T) CCM 4981(T)).

Austria↗
PubMed2002

Chromosome-encoded Ambler class A beta-lactamase of Kluyvera georgiana, a probable progenitor of a subgroup of CTX-M extended-spectrum beta-lactamases.

A chromosome-encoded beta-lactamase gene, cloned and expressed in Escherichia coli from Kluyvera georgiana reference strain CUETM 4246-74 (DSM 9408), encoded the extended-spectrum beta-lactamase KLUG-1, which shared 99% amino acid identity with the plasmid-mediated beta-lactamase CTX-M-8. This work provides further evidence that Kluyvera spp. may be the progenitor(s) of CTX-M-type beta-lactamases.

Amino Acid Sequence↗
PubMed2002

Isolation of Buttiauxella gaviniae from a spinal cord patient with urinary bladder pathology.

A gram-negative Buttiauxella gaviniae-like organism (LBV449) was isolated from a urine sample of a patient suffering from urine bladder pathology and neurological problems. The isolate was positive for adonitol fermentation and L-arginine dihydrolase and negative for melibiose and L-ornithine decarboxylase. The API 20E code was 3004113. Retrospectively, another isolate (ENT107), from a leg wound, was recovered from our collections and was shown to have similar biochemical characteristics. DNA-DNA hybridization showed 77% similarity between both strains, and strain LBV449 revealed 74% DNA-DNA similarity to the type strain of B. gaviniae. Neither 16S rRNA gene sequencing nor fatty acid analysis were useful for identification. The characteristic tRNA-PCR patterns obtained for these two clinical isolates consisted of fragments with lengths of 102.2, 105.4, 116.6, and 136.9 bp and most resembled the tRNA-PCR pattern obtained for B. gaviniae, but they lacked the B. gaviniae fragments of 88.2 and 239.5 bp. To our knowledge, no clinical cases with Buttiauxella strains have been described thus far.

Adult↗