Biomedical subjects

Peter Kofinas

Publications and source records attributed to Peter Kofinas.

3 recordsLinked to original sources

PubMed2006

Molecularly imprinted polymers for tobacco mosaic virus recognition.

Molecular imprinted Polymers (MIP) targeted for Tobacco mosaic virus (TMV) have been synthesized. Batch equilibrium studies using imprinted and non-imprinted polymer hydrogels in TMV and TNV solutions were conducted to determine virus-binding capacities. TMV-imprinted hydrogels showed increased binding to TMV (8.8 mg TMV/gpolymer) compared to non-imprinted hydrogels (4.2 mg TMV/gpolymer). Furthermore, TMV-imprinted hydrogels exhibited increased binding to TMV compared to TNV, while non-imprinted hydrogels bound similar amounts of TMV or TNV. This research has demonstrated that molecular imprinting of viruses can be used to selectively induce binding of target viruses based on shape differences of their virions.

Biocompatible Materials↗

PubMed2004

Biomimetic glucose recognition using molecularly imprinted polymer hydrogels.

Non-covalent molecular imprinting of poly(allylamine hydrochloride) (PAA.HCl) with D-glucose 6-phosphate monobarium salt (GPS-Ba) produced molecularly imprinted polymer hydrogels (MIP) having an affinity to glucose over fructose. The hydrogels were formed by ionic association of the template molecule, GPS-Ba, to the polymer, prior to covalent crosslinking using epichlorohydrin (EPI). The template was removed by an aqueous base wash. Batch equilibration studies using different MIP hydrogels and non-molecularly imprinted polymers (NIPs) were performed in aqueous and buffered media to determine the binding capacities and isomeric selectivities with respect to the sugars, glucose and fructose. MIP glucose hydrogels exhibited binding capacities in excess of 0.6g of glucose per g of dry gel in a 100% DI H(2)O glucose solution, and in a 50-50% glucose-fructose solution mixture. Equilibrium binding capacities of fructose were lower than those observed with respect to glucose, indicating an isomeric preference for the binding of glucose over fructose. These hydrogels demonstrated a remarkable degree of biomimetic sugar recognition to specifically and selectively bind glucose in their swollen state in environments mimicking physiological conditions.

Adsorption↗

PubMed2003

Reactive phosphorus removal from aquaculture and poultry productions systems using polymeric hydrogels.

This work reports on the features of a sorption processes for the ultimate removal and recovery of reactive phosphorus from aquaculture and poultry production wastewater effluents. The sorbent used was a cross-linked polyamine (PAA-HCl) polymeric hydrogel. The PAA-HCl hydrogels were prepared by chemically cross-linking aqueous solutions of linear PAA-HCl chains with epichlorohydrin (EPI). The phosphorus binding capacity of the gels was measured in standard aqueous solutions as a function of ionic strength. Equilibrium PO4(3-), loadings of 100 mg anion/g gel were obtained. The regeneration ability of the gels was demonstrated by release of the bound phosphorus anions upon washing with 1-2 M NaOH solution, providing opportunities to recover and reuse the gel over multiple cycles. The ionic polyamine gels have been demonstrated to be appropriate materials for treating poultry and aquaculture wastewater effluents. Upon treatment phosphorus anion concentrations were reduced to levels suitable for discharge into natural surface waters.

Animals↗