A simple manual rosetting method for absolute CD4+ lymphocyte counting in resource-limited countries.
A CD4 monoclonal antibody which reacts with CD4+ lymphocytes but does not react significantly with monocytes was generated and used to develop a simple method for CD4 count. In a comparison with standard flow cytometry, high correlation was obtained. The developed method can be an alternative to flow cytometry for resource-limited countries.
Antibodies, Monoclonal↗