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Ping Chen

Publications and source records attributed to Ping Chen.

At least 127 records · Page 7Linked to original sources
PubMed2004

[Primary duct closure versus T-tube drainage following laparoscopic choledochotomy].

OBJECTIVE: To evaluate the effects of primary duct closure and T-tube drainage in laparoscopy choledochotomy to treat the common bile duct stones. METHODS: The enrollment of the patients was in accordance with 6 criteria. 55 patients with cholecystolithiasis and secondary common bile duct stones from January 2000 to February 2003 were treated with laparoscopic choledochotomy. The patients were randomly divided into two groups: primary duct closure group and T-tube drainage group. Their all data were recorded and studied prospectively,and patients were followed up after discharge. RESULTS: There were 27 patients and 28 patients in primary duct closure group and T-tube drainage group respectively. The operation time and the results of following up between the two groups had no significant difference. Compared with T-tube drainage group, primary duct closure group had less the total quantity of postoperative transfusion and hospital costs, shorter postoperative hospital stay. The incidence of postoperative complications in primary duct closure group was 11.1% (3/27), and all of them were biliary complications. The incidence of postoperative complications in T-tube drainage group was 28.6% (8/28), and seven of them were biliary complications. The incidence of severe complications that needed reoperations was 10.7% (93/28), and all of them were caused by T-tubes. There was no mortality in two groups. CONCLUSIONS: The primary duct closure in laparoscopic choledochotomy can avoid the deficiency of T-tube drainage, and it is feasible and safe and lower complications in treating the common bile duct stones, so we advocate it in appropriate cases.

Adolescent↗
PubMed2004

Fluoropyrrolidine amides as dipeptidyl peptidase IV inhibitors.

Amides derived from fluorinated pyrrolidines and 4-substituted cyclohexylglycine analogues have been prepared and evaluated as inhibitors of dipeptidyl dipeptidase IV (DP-IV). Analogues which incorporated (S)-3-fluoropyrrolidine showed good selectivity for DP-IV over quiescent cell proline dipeptidase (QPP). Compound 48 had good pharmacokinetic properties and was orally active in an oral glucose tolerance test in lean mice.

Adenosine Deaminase↗
PubMed2004

Proteome comparative analysis of gynogenetic haploid and diploid embryos of goldfish (Carassius auratus).

Recently, it was found that in the gynogenetic haploid and diploid embryos of goldfish, which have exactly the same genome, the haploid condition results in obstruction of gene expression and abnormal development while the diploid embryos have normal gene expression and development. A diploid-dependent regulatory apparatus was proposed to regulate gene expression. To study the difference at the protein expression level of the embryos of haploid and diploid in development, we extracted the total proteins of both the gynogenetic haploid and diploid embryos of goldfish in the same eye formation stage. Two-dimensional polyacrylamide gel electrophoresis was used to separate proteins. The stained gel images were analyzed with the PDQUEST software. A part of protein spots that were differentially expressed in haploid and diploid embryos were identified by matrix assisted laser desorption/ionisation-time of flight-mass spectrometry and database analysis. Sixteen protein spots that were absolutely different (only expressed in diploid embryos but not in haploid embryos or vice versa) and 16 protein spots that were up- and downregulated were identified unambiguously, which include some proteins that are correlative with eyes development, nerve development, developing regulation, cell differentiation, and signal transduction. The different significantly gene expression during embryos developing between diploid and haploid is demonstrated.

Animals↗
PubMed2004

De novo sequencing of tryptic peptides sulfonated by 4-sulfophenyl isothiocyanate for unambiguous protein identification using post-source decay matrix-assisted laser desorption/ionization mass spectrometry.

A simple method of solid-phase derivatization and sequencing of tryptic peptides has been developed for rapid and unambiguous identification of spots on two-dimensional gels using post-source decay (PSD) matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The proteolytic digests of proteins are chemically modified by 4-sulfophenyl isothiocyanate. The derivatization reaction introduces a negative sulfonic acid group at the N-terminus of a peptide, which can increase the efficiency of PSD fragmentation and enable the selective detection of only a single series of fragment ions (y-ions). This chemically assisted method avoids the limitation of high background normally observed in MALDI-PSD spectra, and makes the spectra easier to interpret and facilitates de novo sequencing of internal fragment. The modification reaction is conducted in C(18) microZipTips to decrease the background and to enhance the signal/noise. Derivatization procedures were optimized for MALDI-PSD to increase the structural information and to obtain a complete peptide sequence even in critical cases. The MALDI-PSD mass spectra of two model peptides and their sulfonated derivatives are compared. For some proteins unambiguous identification could be achieved by MALDI-PSD sequencing of derivatized peptides obtained from in-gel digests of phosphorylase B and proteins of hepatic satellite cells (HSC).

Amino Acid Sequence↗
PubMed2004

Bone marrow mononuclear cell transplantation into heart elevates the expression of angiogenic factors.

The expression of angiogenic factors was measured to elucidate the mechanism of angiogenesis after bone marrow stem cells transplantation. The left anterior descending coronary (LAD) artery was ligated in male Lewis rats to induce an acute myocardial infarction (AMI); of which, some rats received bone marrow mononuclear cells transplantation (BMT). The expression of cytokines and their receptors was assessed by RT-PCR. The expression of c-met was upregulated in the AMI animals and was further increased when the animals received BMT (P < 0.01). The other cytokines and their receptors remained unchanged after BMT compared with AMI samples. Normal bone marrow mononuclear cells have higher expression level of hepatocyte growth factor (HGF) than those in normal hearts. In conclusion, BMT could increase the expression of c-met. Not all angiogenic cytokines and their receptors were upregulated after BMT. The higher expression of hepatocyte growth factor in bone marrow may contribute to the upregulation of c-met.

Angiogenesis Inducing Agents↗
PubMed2004

Metabolism of anandamide in cerebral microvascular endothelial cells.

Anandamide (N-arachidonoylethanolamine, AEA), an endogenous cannabinoid receptor agonist, causes potent vasodilation in the cerebral circulation through an endothelial-dependent or -independent mechanism. We have investigated the processing of [3H]AEA in cultured mouse cerebral microvascular endothelial cells (MEC) in order to better understand its mechanism of action in the cerebral vasculature. These cells took up anandamide very quickly, reaching a maximum value in 5 min and remaining at that level for at least 8 h. Analysis of the cell lipids demonstrated that, in addition to free anandamide, radioactivity was incorporated into phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidylethanolamine (PE) in a time-dependent manner. Analysis of the hydrolyzed cell lipids indicated that anandamide was converted to arachidonic acid, a process that was inhibited by the selective fatty acid amide hydrolase inhibitor oleyl trifluoromethyl ketone (OTMK). Phospholipase A2 (PLA2) hydrolysis of the PC, PI, and PE fractions indicated that the arachidonic acid formed from anandamide was esterified predominately into sn-2 position of the endothelial phospholipids. Furthermore, anandamide and arachidonic acid were released when the cells were incubated with A23187. These results suggest that the biological activity of anandamide might be regulated by its rapid uptake and calcium-dependent release in endothelial cells, and conversion of anandamide to arachidonic acid might serve as an inactivation process in the cerebral microcirculation.

Amidohydrolases↗
PubMed2004

Differential analysis of two-dimension gel electrophoresis profiles from the normal-metaplasia-dysplasia-carcinoma tissue of human bronchial epithelium.

Processes involved in malignant transformation of the lung from preneoplasia are poorly understood. To better understand this process, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) profiles of proteins from the normal, metaplasia, dysplasia and carcinoma tissues of human bronchial epithelia were examined by differential proteomic analysis. The selected differential protein-spots were identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database searching. The average spots for normal epithelium, metaplasia, dysplasia and invasive carcinoma were 1189.50 +/- 39.89, 1227.00 +/- 37.90, 1273.00 +/- 43.31 and 1326.00 +/- 66.63, respectively. Well-resolved, reproducible 2-D PAGE patterns of the normal-metaplasia-dysplasia-carcinoma tissues of bronchial epithelia were obtained. After matching, the number of spots of differential proteins between normal tissue and metaplasia, metaplasia and dysplasia, and dysplasia and invasive cancer tissues were 31.50 +/- 7.67, 41.00 +/- 9.07 and 56.00 +/- 8.96, respectively. In total, 35 differential proteins, expressed only at the later stage of a two-stage comparison, were identified, some of which are known to be involved in regulating the processes of proliferation, differentiation and signal transduction. Current data in this study, for the first time, provide the basis for identification of potential tumor markers of human lung squamous carcinoma and their involvement in the progression of malignant transformation of bronchial epithelium.

Bronchi↗
PubMed2004

Attenuation of late-stage disease in mice infected by the Mycobacterium tuberculosis mutant lacking the SigF alternate sigma factor and identification of SigF-dependent genes by microarray analysis.

The Mycobacterium tuberculosis alternate sigma factor, SigF, is expressed during stationary growth phase and under stress conditions in vitro. To better understand the function of SigF we studied the phenotype of the M. tuberculosis DeltasigF mutant in vivo during mouse infection, tested the mutant as a vaccine in rabbits, and evaluated the mutant's microarray expression profile in comparison with the wild type. In mice the growth rates of the DeltasigF mutant and wild-type strains were nearly identical during the first 8 weeks after infection. At 8 weeks, the DeltasigF mutant persisted in the lung, while the wild type continued growing through 20 weeks. Histopathological analysis showed that both wild-type and mutant strains had similar degrees of interstitial and granulomatous inflammation during the first 12 weeks of infection. However, from 12 to 20 weeks the mutant strain showed smaller and fewer lesions and less inflammation in the lungs and spleen. Intradermal vaccination of rabbits with the M. tuberculosis DeltasigF strain, followed by aerosol challenge, resulted in fewer tubercles than did intradermal M. bovis BCG vaccination. Complete genomic microarray analysis revealed that 187 genes were relatively underexpressed in the absence of SigF in early stationary phase, 277 in late stationary phase, and only 38 genes in exponential growth phase. Numerous regulatory genes and those involved in cell envelope synthesis were down-regulated in the absence of SigF; moreover, the DeltasigF mutant strain lacked neutral red staining, suggesting a reduction in the expression of envelope-associated sulfolipids. Examination of 5'-untranslated sequences among the downregulated genes revealed multiple instances of a putative SigF consensus recognition sequence: GGTTTCX(18)GGGTAT. These results indicate that in the mouse the M. tuberculosis DeltasigF mutant strain persists in the lung but at lower bacterial burdens than wild type and is attenuated by histopathologic assessment. Microarray analysis has identified SigF-dependent genes and a putative SigF consensus recognition site.

Animals↗
PubMed2004

Expression of matrix metalloproteinase 9 in nasopharyngeal carcinoma and association with Epstein-Barr virus infection.

OBJECTIVE: To evaluate the expression of matrix metalloproteinase-9 (MMP9) in nasopharyngeal carcinoma and the association between MMP9 and Epstein-Barr virus infection. METHODS: The MMP9 expression was studied by immunohistochemical analysis; and Epstein-Barr virus encoded small nuclear mRNA-1 (EBER-1) produced by in situ hybridization were examined in 41 nasopharyngeal carcinoma sections, and the relation between them, and the associations of MMP9 with clinical features were statistically analyzed. RESULTS: Positive expression rate of MMP9 was 73.17%. The expression of MMP9 showed significant positive correlation with the expression of EBER-1 (gamma=0.483, P=0.001). There was significant association of MMP9 expression with lymph nodes metastasis and clinical stage (P<0.001), non-significant association with age, gender, pathological classification and T classification. CONCLUSIONS: The highly pronounced expression of MMP9 is associated with cervical lymph nodes metastasis. Epstein-Barr virus can enhance NPC metastasis by up-regulating the expression of MMP9.

Adult↗
PubMed2004

Cytological changes in association with ethoxyresorufin o-deethylase induction in fish upon dietary exposure to benzo[a]pyrene.

Juvenile areolated grouper (Epinephelus areolatus) were exposed to two levels of dietary benzo[a]pyrene (BaP; 0.25-12.5 microg/g body wt/d) for four weeks, followed by four weeks of depuration. Significant increase in hepatic ethoxyresorufin O-deethylase (EROD) activities was found after one week, preceding an increase in lipopigments (as measured by quantitative transmission electron microscopy) in week 2 of exposure. The EROD activities in the BaP-treated fish subsided at week 4 of exposure and throughout the depuration period. Lipopigments in the high-dose group appeared to be more persistent than that of the EROD activity during the exposure period and remained significantly higher than that of the controls at week 4. Levels of lipopigments, however, rapidly subsided on withdrawal of BaP exposure. These results appear to suggest that changes in EROD activities would precede cytological changes and that both the observed cytological and biochemical changes are reversible. Results of the present study also lend further support to our earlier findings on Solea ovata, that a significant relationship exists between EROD activity and lipopigment accumulation (as measured by volume density, absolute volume, numerical density, and absolute density; r = 0.483-0.358, p < 0.05), regardless of fish species (S. ovata and aerolated grouper) as well as the routes of exposure to BaP (intraperitoneal injection or dietary exposure). This provides strong supporting evidence that elevated EROD activities in fish liver do not merely indicate exposure to polyaromatic hydrocarbons (PAHs) but are also associated with significant biological effects. Our results showed that hepatic EROD activity and lipopigments could be used to indicate recent exposure of the fish to BaP/PAHs.

Administration, Oral↗
PubMed2004

[Differential analysis of two-dimension gel electrophoresis profiles of human lung squamous carcinoma and tumor-adjacent tissue].

BACKGROUND & OBJECTIVE: Carcinogenesis of lung squamous carcinoma is a complex process involving multiple events and steps. Though some molecular pathogenesis studies on human lung cancer have been undertaken successfully in gene (DNA) and transcription (mRNA) levels, the carcinogenic mechanism is still unclear. At present, there is no special molecular marker for early-stage diagnosis and prognosis evaluation. The objective of this study was to establish two-dimensional electrophoresis profiles with high resolution and reproducibility from human lung squamous carcinoma tissue and paired normal tumor-adjacent bronchial epithelial tissue, and to identify differential expression proteins. METHODS: The total proteins of human lung squamous carcinoma tissue and paired normal tumor-adjacent bronchial epithelial tissue were separated by immobilized pH gradient (IPG)-based two-dimensional gel electrophoresis (2-DE). The differential expression proteins were analyzed using image analysis software, then identified using mass spectrometry and database searching. RESULTS: (1) For tumor tissues, the average protein spots of 3 gels were 1349+/-67; and 1235+/-48 spots were matched with the average matching rate of 91.5%. For control, the average protein spots of 3 gels were 1297+/-73; and 1183+/-56 spots were matched with the average matching rate of 91.2%. The average position deviation of matched spots was 0.873+/-0.125 mm in IEF direction, and 1.025+/-0.213 mm in SDS-PAGE direction. (2) A total of 1069+/-45 spots were matched between the electrophoretic maps of 15 human lung squamous carcinoma tissue and paired normal tumor-adjacent bronchial epithelial tissue. Forty differential proteins were identified by peptide mass fingerprint(PMF), some proteins were the products of oncogenes, and the others involve in the regulation of cell cycle and signal transduction. CONCLUSION: In this study, the well-resolved, reproducible 2-DE patterns of human lung squamous carcinoma and adjacent normal bronchial epithelial tissues were established and certain differential proteins were characterized. These data will be helpful for screening the biomarker to further study on human lung squamous carcinoma.

Amino Acid Sequence↗
PubMed2004

[Hydrogen peroxide upregulates interleukin-1beta-induced cyclooxygenase-2 expression in human pulmonary epithelial cells].

OBJECTIVE: To investigate the effect of hydrogen peroxide (H(2)O(2)) on interleukin-1beta (IL-1beta)-induced cyclooxygenase-2 (COX-2) expression in human pulmonary epithelial cells (HPEC). METHODS: HPEC were stimulated by IL-1beta or H(2)O(2) or both of them, COX-2 mRNA expression was determined by reverse transcription-polymerase chain reaction (RT-PCR), and prostaglandin E(2) (PGE(2)) release was detected by enzyme-linked immunosorbent assay (ELISA). Cells that were not stimulated by any reagent were used as controls. RESULTS: (1) The relative amount of COX-2 mRNA expression induced by 1, 5 and 10 mg/L of IL-1beta [(143.1 +/- 7.2)%, (179.9 +/- 9.0)%, (190.0 +/- 9.5)%, respectively] was significantly higher than that in the control group (32.9 +/- 1.7)% (P < 0.05, respectively). (2) The concentration of PGE(2) released by HPEC treated with 5 and 10 mg/L of IL-1beta [(20.86 +/- 5.23) x 10(-6) g/L, (31.16 +/- 2.64) x 10(-6) g/L, respectively] was significantly higher than that in the control group (10.49 +/- 0.36) x 10(-6) g/L (P < 0.05, respectively). (3) The relative amount of COX-2 mRNA expression induced by 0.10, 0.25 and 0.50 mmol/L of H(2)O(2) in the presence of IL-1beta [(149.2 +/- 7.5)%, (189.6 +/- 9.5)%, (239.1 +/- 12.0)%, respectively] was significantly higher than that in the cells treated with IL-1beta alone (66.1 +/- 3.7)% or that in the control group (41.6 +/- 2.1)% (P < 0.05, respectively). (4) The concentration of PGE(2) released by HPEC treated with 0.10, 0.25 and 0.50 mmol/L of H(2)O(2) in the presence of IL-1beta [(27.01 +/- 5.16) x 10(-6) g/L, (32.79 +/- 3.01) x 10(-6) g/L, (41.13 +/- 3.41) x 10(-6) g/L, respectively] was significantly higher than that in the control group (10.49 +/- 0.36) x 10(-6) g/L (P < 0.05, respectively). The concentration of PGE(2) released by cells treated with 0.25 or 0.50 mmol/L of H(2)O(2) in the presence of IL-1beta was significantly higher than that in the cells treated with IL-1beta alone (20.86 +/- 5.23) x 10(-6) g/L (P < 0.05, respectively). CONCLUSION: Hydrogen peroxide upregulates IL-1beta-induced COX-2 expression in HPEC at transcriptional level.

Cell Line↗
PubMed2004

[Application of floating tube in dilation of eustachian tube under nasal endoscopy to treat post-irradiation secretory otitis media].

OBJECTIVE: To analyse the function of eustachian tube in post-irradiation secretory otitis media and investigate the curative effect of dilating eustachian tube with Swan-Gans thermodilution catheter for treating post-irradiation secretory otitis media in NPC patients. METHOD: Twenty-one (37 ears) post-irradiation secretory otitis media patients with NPC were selected in this study, by using eustachian tube function synthetic inspection facility (JK-04A model), the eustachian tube function was examined, and the obstructive eustachian tube was dilated with Swan-Gans thermodilution catheter under nasal endoscopy. RESULT: The eustachian tube function in 37 ears with post-irradiation secretory otitis media reveals normal, obstructive, insufficiency and opening model was 2, 21, 8, and 6 respectively. The total curative effect of dilating eustachian tube with Swan-Gans thermodilution catheter to treat post-irradiation secretory otitis was 43.2% (16/37). CONCLUSION: The eustachian tube function in post-irradiation secretory otitis media mainly reveals obstructive disturbance. The application of Swan-Gans thermodilution catheter in dilation of obstructive eustachian tube under nasal endoscopy will increase the curability of post-irradiation secretory otitis media.

Adult↗
PubMed2004

[Influence of peroxisome proliferator-activated receptor gamma on airway inflammation of guinea pigs with asthma].

OBJECTIVE: To investigate the influence and mechanism of peroxisome proliferator-activated receptor gamma (PPAR-gamma) and its ligand agonist rosiglitazone on airway inflammation of guinea pigs with asthma. METHODS: 35 guinea pigs were divided into 5 groups by random number meter: a control group (A group), an asthma group (B group), a dexamethasone (DXM) group (C group), a rosiglitazone group (D group), and a rosiglitazone + 1/2 DXM group (E group), with 7 guinea pigs in each. Bronchoalveolar lavage (BAL) cell count and differential was studied, and the pathologic alteration of the bronchi and the lung tissue was observed. The expression of PPAR-gamma, COX-2 was measured by RT-PCR. RESULTS: BAL eosinophil count was 0.050 +/- 0.020 in D group, 0.110 +/- 0.020 in B group, the difference being significantly (t = 5.61, P < 0.001). The total cell number and neutrophils were (15.5 +/- 3.9) x 10(8)/L and 0.069 +/- 0.020 in D group, and (19.9 +/- 4.3) x 10(8)/L and 0.076 +/- 0.020 in B group, the difference being not significant (t = 2.02, 0.66, P > 0.05 respectively). The thickness of airway wall of D group was (22.0 +/- 5.0) micro m, and in B group it was (28.0 +/- 5.0) micro m, the difference being significant (t = 2.61, P < 0.05), but the thickness of mucosa and submucosa of D group (12.2 +/- 2.9) micro m was not different as compared with B group (14.9 +/- 3.3) micro m (t = 1.63, P > 0.05). Expression of PPAR-gamma mRNA of D group 19.5 +/- 3.0 was not different compared with B group 18.1 +/- 3.1 and A group 15.6 +/- 2.9 respectively (t = 0.92, 0.49, P > 0.05, respectively). When the expression of COX-2 mRNA of B group 49 +/- 7 was compared with D group 39 +/- 6, the difference was significant (t = 2.77, P < 0.05). CONCLUSIONS: Rosiglitazone decreases airway eosinophils and the thickness of airway wall in guinea pigs with asthma, via suppression of COX-2 mRNA expression by activating PPAR-gamma. The anti-inflammatory effect of PPAR-gamma may be associated with the use of ligand agonist and(or) glucocorticoids.

Animals↗
PubMed2004

Recombinant fibronectin polypeptide antagonizes hepatic failure induced by endotoxin in mice.

AIM: To study the preventive effect of recombinant human fibronectin (rhFN) polypeptide on hepatic failure induced by endotoxin in mice. METHODS: A cDNA fragment coding for Ile1363-Tyr1725 of human FN was inserted into the PinPoint Xa-3 plasmid, and the constructed plasmid was transformed into E coli BL21 (DE3) cells, and then the expression of rhFN polypeptide in DE3 cells was identified through SDS-PAGE. The target protein from the supernatant of bacteria lysate was purified through biotin-affinity chromatography. The bioactivity of the purified rhFN polypeptide was determined with cell adhesive activity. The survival rate was observed in endotoxemia mice injected with rhFN polypeptide. The tissue damage in hepatocyte was detected using histology, ultrastructure, and DNA fragmentation assay. RESULTS: The expression of rhFN polypeptide reached approximately 20 % of the total cellular protein. The adhesion ability of rhFN polypeptide was concentration-dependent. The value of EC(50) was 0.8 nmol/L. The survival rate of endotoxemia mice sensitized by D-galactosamine (D-GalN) was 60 % in rhFN polypeptide treated group, while that of endotoxemia mice sensitized by D-GalN was 20 % in the control group (P<0.01). Histopathology showed that less necrosis occurred on the hepatocyte of endotoxemia mice injected with rhFN polypeptide compared with saline control. Ultrastructure and DNA fragmentation assay showed that no apoptotic hepatocyte was observed in the liver of rhFN-treated endotoxemia mice. CONCLUSION: Recombinant fibronectin polypeptide antagonizes hepatic failure induced by endotoxin in mice.

Animals↗
PubMed2004

[Homozygous hemoglobin constant spring in Guangxi province].

OBJECTIVE: To analyze the relationship between genotype and phenotype of homozygous hemoglobin Constant Spring (Hb CS) in Guangxi province, and to explore the reasons of missed diagnosis and the methods for screening and diagnosing. METHODS: Screening Hb CS by acetate fibrous membrane electrophoresis with benzidine staining. Gene mutation of homozygous Hb CS by polymerase chain reaction (PCR) and DNA sequencing. RESULTS: Out of the 9 patients, 4 had no clinical symptoms, 2 showed mild anemia and splenomegaly, 3 were jaundice. Hemoglobin levels were normal or mild anemia. MCVs were normal or reduced. Peripheral blood smear of all the patients displayed hypochromia, anisocytosis, poikilocytosis and target cells. The quantities of HbA(2) + Hb CS were 4.3% - 6.72%, while HbA(2) < 2%. Gene analysis confirmed the diagnosis of homozygous Hb CS. CONCLUSION: There was quite different in clinical symptoms, hematological parameters and hemoglobin quantifications for patients with homozygous Hb CS. They might have no clinical and hematological signs and looked like the phenotype of Hb H disease. Homozygous Hb CS was very easy to miss diagnosis. Gene analysis can be helpful.

Adult↗
PubMed2004

[CD86 expression on alveolar macrophages and effects of anti-CD86 monoclonal antibody on the production of inflammatory cytokines in dust-mite allergic asthma patients].

OBJECTIVE: To investigate the CD(86) molecule expression on alveolar macrophages (AMs) before and after AM activation with dust-mite allergen and effects of anti-CD(86) monoclonal antibody on the production of inflammatory cytokines produced by cultured CD(34)(+) T cells, and to explore the pathogenesis of anti-CD(86) monoclonal antibody on inhibiting asthmatic inflammation. METHODS: AMs and CD(34)(+) T cells were isolated from bronchoalveolar lavage fluid (BALF) and from peripheral blood derived from 10 asthma patients allergic to house dust-mite dermatophagoides pteronyssinus. AMs were stimulated and activated by house dust-mite allergen and co-cultured with CD(34)(+) T cells. The co-cultured cells were divided into anti-CD(86) monoclonal antibody intervention group and CD(86) expression group with which the control groups were set up. The anti-CD(86) antibody was added to 0.1 mg/L and 1.0 mg/L in the experiment group and no antibody was added in the control group. The supernatants were harvested after 72 hour co-culture. The concentration of interferon-gamma (INF-gamma), interleukin-4 (IL-4) and IL-5 were determined by enzyme linked immunosorbent assay (ELISA). CD(86) expression group was divided into experiment and control groups, and house dust-mite allergen was added to the experiment group but no allergen was added in the control group. The cells of the two groups were harvested after 24 hour co-culture. The CD(86) molecule expression on the AMs was detected by flow cytometry. RESULTS: After activated by house dust-mite allergen, the average fluorescence density of CD(86) on AM in the experiment group [(50 +/- 9)%] was significantly higher than that in the control group [(23 +/- 5)%, P < 0.01]. The production of IL-4 and IL-5 was (135 +/- 19) ng/L, and (104 +/- 21) ng/L in the low-dose group respectively; (90 +/- 17) ng/L and (68 +/- 14) ng/L in the high-dose group; (187 +/- 24) ng/L and (161 +/- 23) ng/L in the control group; the differences among groups were significant (P < 0.01). The production of IFN-gamma was (193 +/- 39) ng/L in the low-dose group and (201 +/- 47) ng/L in the high-dose group respectively, and there was no significant difference between the two groups (P > 0.05). CONCLUSION: AMs may effectively present allergen to T cells in allergic asthma patients. Anti-CD(86) monoclonal antibody can inhibit the production of IL-4 and IL-5 by blocking the co-stimulatory signal of CD(34)(+) T cells. These data suggest that anti-CD(86) monoclonal antibody may hold therapeutic potentials in asthma by inhibiting airway inflammation.

Antibodies, Monoclonal↗