Biomedical subjects

Pingjian Deng

Publications and source records attributed to Pingjian Deng.

8 recordsLinked to original sources

PubMed2005

[Evaluation on construction and expression in vitro of nucleic acid vaccine of nucleoprotein of influenza virus A].

OBJECTIVE: Constructing nucleic acid vaccine of influenza virus A to study the protective effects. METHODS: NP gene was reverse transcripted from influenza virus A and linked with pSECTAG 2/Hygro A to constructed nucleic acid vaccine of influenza virus A. The constructed nucleic acid vaccine was transinfected into VERO cell resorting to lipofectamineTM2000. NP gene of influenza virus A was expressed in VERO cell by the method of ELISA. RESULTS: The results showed that the NP gene was expressed to the highest level at 36h after transinfection with the A40 value of 0.382. From 36 h to 60 h after transinfection, the expression of NP gene was stable (the A45, value at 48h and 60h was 0.385 and 0.387, respectively) . CONCLUSION: The nucleic acid vaccine of influenza virus A was successfully constructed, which sets up groudworks for the research of effection of nucleic acid vaccine in vivo.

Animals↗

PubMed2004

[Expression and identification of cecropin D gene cloned in Pichia pastoris yeast cells].

OBJECTIVE: Cecropin D gene was cloned into methanol nutritional vector-pPICZ alpha-A, linked with alpha-factor signal peptide. METHODS: Positive recombinant was gotten through PCR and EcoR I restriction enzyme. The positive recombinant vector was transformed into Pichia pastoris yeast strain GS115. RESULTS: The positive recombinant yeast velum was fermented and its expressive product was assayed by the method of anti-germ. CONCLUSION: Cecropin D gene was cloned into yeast cell and its expressive products has high activity of killing bacteria, Which could be developed into a new type of anti-bacterial medicine.

Anti-Bacterial Agents↗

PubMed2004

[Mechanism and prevention measures of gene silencing].

With the widely application of transgene technology in the fields of high-level biologies, more and more attention was payed to gene silence. Gene silence can take place at the phase of transcription and post-transcription. With the results of methylation, repeated copies and homologous sequence of inner genes, trans-gene are silenced. There are many kinds of mechanisms of gene silencing and here, the mechanisms explaining the gene silence and some prevention measures were summarized in this paper.

DNA Methylation↗

PubMed2004

[Safety assessment of GM yeast feed additive with cecropin CAD gene].

OBJECTIVE: To evaluate the safety of GM yeast feed additive with cecropin CAD and to study and set up a model of Safety assessment for GM feed and detecting method. METHODS: To ensure the safety of the GM products, it has been done that to detect and value the safety of receptor organisms and expression products of extrinsic gene, the genetic stability of biologic properties of genomic modified yeast feed and condition of transfer and cumulation of anti-bacterial peptide and its products in circumstance and the feeded animals. RESULT AND CONCLUSION: The receptor animals and expression products of extrinic gene are safe, and the genomic modified products have steady genetic characters. The cectopin CAD neither cumulates in feeded animal nor releases into environment. The genomic modified feed additive is safe.

Animal Feed↗

PubMed2003

[Studies on protein-based identification method of genetically modified capsicum].

The detection system based on protein is a method to evaluate the safety of genetically modified foods (GMF). Using cecropin BD gene in capsicum, a detecting method was set up. It is a system of evaluating the real expressive condition and safety of the foreign target protein of GMF. In this studies, with the preformative technic method, a satisfactory results by making use of hemolymph of immunized pupae of Antheraea pernyi as standard experimental material was achieved, comparing with the realities of the goal protein expressive condition of cecropin D gene in capsicum. The detecting steps were as following: the goal protein from material was extracted roughly, then with CM-Sepharose-FF ion-exchange chromatography twice, the goal protein was purified moderately. The purified product was identified by detecting the anti-bacterial activity, electrophoresis, biological auto-photography of the goal protein and MADDI-TOF mass spectrum. The results showed that the expressive foreign target protein in transgenic capsicum was in accordance with standard protein in the physical and chemical property, anti-bacterial activity and molecular weight. It indicated that expression of the target gene in capsicum is real, it corresponded to expected value. The separation, purification and identification methods of cecropin D were established in the study. By means of the comparative experiments about anti-bacterial activity and molecular weight of anti-bacterial peptide(ABP) from GM-capsicum and hemolymph of immunized pupae of Antheraea pernyi, the identification method of target protein from GM-capsicum was set up. The method is easy to be operated, fast and feasible.

Capsicum↗

PubMed2003

[Gene chip technology and it's application to genetic toxicology].

Gene chip, also called as DNA array, Oligonucleotide Microchip or Oligonucletide array, is a new technology developed in recent years. It can be applied prospectively in life science research, such as gene expression, gene diagnose and gene polymorphism etc. In this paper, the principle, types, procedures and application of gene chip to genetic toxicological research were reviewed.

Humans↗

PubMed2003

[Application of mass spectrometry in the proteome research].

Electrospray ionization(ESI) and matrix-assisted laser desorption ionization(MALDI) are two kinds of newly-developed mass spectrometry techniques. The application of mass spectrometry is described in the proteome research such as MS charting, determination of molecular weight, peptide mass fingerprinting(PMF) and peptide sequence validation. The advantages of mass spectrometry are sensitive, accurate, rapid, high-throughput and automated. They have been proved a powerful technique and can be used as an important method for proteome research.

Mass Spectrometry↗

PubMed2002

[Study on the identification of genomic modified foods].

Nucleotide-based amplification method is an important system for the identification of genomic modified foods (GMF). Roundup Ready Soybeans (Monsanto company), Bt 176 GM maize (Novartis/Ciba-Geigy company) and Cecropin D capsicum was used as material to search for the feasibility of investigating the safety of GMF by PCR method. Primers specific for inserted genes and crop endogenous genes in Roundup Ready Soybeans, Bt 176 maize and Cecropin D capsicum were applied. The discrimination system for GM soybeans, GM maize and GM capsicum from the counterpart of non-GM products and the detection system for correlating marker genes and transgenes are established. The method was easy and fast, and the corresponding results fixed the standard or declared data.

Capsicum↗