Biomedical subjects

Prashant Padmawar

Publications and source records attributed to Prashant Padmawar.

3 recordsLinked to original sources

PubMed2006

Millisecond association kinetics of K+ with triazacryptand-based K+ indicators measured by fluorescence correlation spectroscopy.

We recently introduced a water-soluble, long-wavelength K(+)-sensing indicator, TAC-Red, consisting of a triazacryptand K(+)-selective ionophore coupled to a xanthylium chromophore (Nat. Methods 2005, 2, 825-827). Stopped-flow kinetic analysis indicated that in response to changes in K(+) concentration TAC-Red fluorescence enhancement occurs in milliseconds or less. Here, we use fluorescence correlation spectroscopy to quantify the binding kinetics of K(+) with TAC-Red and a new, longer-wavelength sensor, TAC-Crimson. Autocorrelation functions, G(tau), were similar at 0 and high (150 mM) K(+) concentrations, with the appearance of a prominent kinetic process with a correlation time in the millisecond range for K(+) concentrations between approximately 20 and 60 mM. Control experiments with increased illumination volume and solution viscosity indicated that the millisecond component represented K(+)/TAC-Red association. K(+)-dependent G(tau) data, modeled using a global regression to a binding/diffusion model, gave association and dissociation rate constants of 0.0020 +/- 0.0003 mM(-1) ms(-1) and 0.12 +/- 0.02 ms(-1), respectively, for TAC-Red. Similar results were obtained for TAC-Crimson. The rapid K(+) binding kinetics with triazacryptand-based sensors support their utility for measuring changes in K(+) concentrations during rapid neural signaling and ion channel gating.

Cations↗

PubMed2006

Photoinduced processes in a tricomponent molecule consisting of diphenylaminofluorene-dicyanoethylene-methano[60]fullerene.

Photoinduced intramolecular processes in a tricomponent molecule C60(>(CN)2-DPAF), consisting of an electron-accepting methano[60]fullerene moiety (C60>) covalently bound to an electron-donating diphenylaminofluorene (DPAF) unit via a bridging dicyanoethylenyl group [(CN)2], were investigated in comparison with (CN)2-DPAF. On the basis of the molecular orbital calculations, the lowest charge-separated state of C60(>(CN)2-DPAF) is suggested to be C60*-(>(CN)2-DPAF*+) with the negative charge localized on the fullerene cage, while the upper state is C60(>(CN)2*--DPAF*+). The excited-state events of C60(>(CN)2-DPAF) were monitored by both time-resolved emission and nanosecond transient absorption techniques. In both nonpolar and polar solvents, the excited charge-transfer state decayed mainly through initial energy-transfer process to the C60 moiety yielding the corresponding 1C60, from which charge separation took place leading to the formation of C60*-(>(CN)2-DPAF*+) in a fast rate and high efficiency. In addition, multistep charge separation from C60(>(CN)2*--DPAF*+) to C60*-(>(CN)2-DPAF*+) may be possible with the excitation of charge-transfer band. The lifetimes of C60*-(>(CN)2-DPAF*+) are longer than the previously reported methano[60]fullerene-diphenylaminofluorene C60(>(C=O)-DPAF) with the C60 and DPAF moieties linked by a methanoketo group. These findings suggest an important role of dicyanoethylenyl group as an electron mediating bridge in C60(>(CN)2-DPAF).

Journal Article↗

PubMed2005

K+ waves in brain cortex visualized using a long-wavelength K+-sensing fluorescent indicator.

We synthesized a water-soluble, long-wavelength K(+) sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0-50 mM K(+) with K(+)-to-Na(+) selectivity >30. We visualized K(+) waves in TAC-Red-stained brain cortex in mice during spreading depression, with velocity 4.4 +/- 0.5 mm/min, and K(+) release and reuptake half-times (t(1/2)) of 12 +/- 2 and 32 +/- 4 s, respectively. Aquaporin-4 (AQP4) deletion slowed K(+) reuptake about twofold, suggesting AQP4-dependent K(+) uptake by astroglia.

Animals↗