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Biomedical subjects

Q Chen

Publications and source records attributed to Q Chen.

At least 19 recordsLinked to original sources

Normal and shear strain estimation using beam steering on linear-array transducers.

In current ultrasound elastography, only the axial component of the displacement vector is estimated and used to produce strain images. A method was recently proposed by our group to estimate both the axial and lateral components of a displacement vector following a uniaxial compression. Previous work evaluated the technique using both simulations and a mechanically translated phased array transducer. In this paper, we present initial results using beam steering on a linear array transducer attached to a commercial scanner to acquire echo signals for estimating 2-D displacement vectors. Single-inclusion and anthropomorphic breast phantoms with different boundary properties between the inclusion and background material are imaged by acquiring echo data along beam lines ranging from -15 degrees to 15 degrees relative to the compression direction. 1-D cross-correlation is used to calculate "angular displacements" in each acquisition direction, yielding axial and lateral components of the displacement vector. Strain tensor components are estimated from these displacements. Features on shear strain images generated for the inclusion phantom agree with those predicted using FEA analysis. Experimental results demonstrate the utility of this technique on clinical scanners. Shear strain tensors obtained using this method may provide useful information for the differentiation of benign from malignant tumors. For the linear array transducer used in this study, the optimum angular increment is around 3 degrees. However, more work is required for the selection of an appropriate value for the maximum beam angle for optimal performance of this technique.

Humans↗

Activated endothelial cells resist displacement by trophoblast in vitro.

BACKGROUND: Transformation of the spiral arteries by invading trophoblasts is an essential prerequisite to the development of a healthy fully grown fetus. Reduced transformation of the spiral arteries is a characteristic feature of pregnancies complicated by several diseases of pregnancy including preeclampsia. The aim of this study was to investigate further the hypothesis that spiral artery endothelial cells can contribute to the mechanism of shallow trophoblast invasion. METHOD: Fluorescently labeled Jar cells were added to monolayers of fluorescently-labeled endothelial cells that had been activated by treatment with TNFalpha, INF gamma or necrotic cell bodies. The ability of the Jars to displace endothelial cells from the monolayers was quantified by measuring the area of Jar cells "islands" formed in the endothelial cell monolayers by confocal microscopy and digital image. RESULTS: The area of Jar cell islands formed in monolayers of activated endothelial cells was significantly smaller that the area of islands formed in control resting/non-activated endothelial cell monolayers regardless of the activator. DISCUSSION: This work demonstrates that activated endothelial cells are more resistant to trophoblast displacement than resting endothelial cells and adds weight to the suggestion that endothelial cells could contribute to shallow invasion of the spiral arteries by trophoblasts in diseases such as preeclampsia.

Cell Line↗

Clinic-based evaluation of Clearview Chlamydia MF for detection of Chlamydia trachomatis in vaginal and cervical specimens from women at high risk in China.

OBJECTIVES: To determine the performance of a rapid Chlamydia trachomatis (CT) test (Clearview Chlamydia MF) compared to the current "gold standard" (Roche Amplicor CT assay) test, and to assess acceptability of the tests to patients. METHODS: A total of 1497 women at sexually transmitted diseases (STD) clinics or re-education centres in six urban cities (Shanghai, Nanjing, Shenzhen, Guangzhou, Chengdu and Fuzhou) in China participated in the study. Three vaginal and three cervical swabs were collected from each participant. Rapid CT tests were performed locally on the first vaginal and cervical swabs and the results were read independently by two staff members. The second and third swabs were randomised for performing the Roche CT assay at the National STD Reference Laboratory. Acceptability of the rapid tests to patients was determined by asking patients in clinics about their willingness to wait for the results. RESULTS: The prevalence of CT was 13.2% (197/1497), as determined by the Roche assay with cervical specimens. CT was detected in 78 vaginal and 127 cervical specimens by the rapid test and the positive rates determined with cervical specimens were significantly higher than those with vaginal specimens (p<0.001). There was good agreement between the results read by two independent staff for either vaginal or cervical specimens (both kappa = 0.98, p<0.001). Sensitivities for vaginal and cervical specimens were 32.8% and 49.7%, respectively, and specificities were 99.2% and 97.9%, respectively. The positive predictive value was 85.7% for vaginal and 78.4% for cervical specimens. The vast majority of the patients (99.1%) were willing to wait up to two hours for the results. CONCLUSION: Clearview Chlamydia MF, while yielding a rapid result and requiring minimal laboratory facilities, had unacceptably low sensitivity compared to a nucleic acid amplification test. Rapid tests yielding results within one hour are generally accepted by the clients.

Adult↗

Effect of H2 on the electrical transport properties of single Bi2S3 nanowires.

Field effect transistors have been fabricated using Bi2S3 nanowires. Whether the contact is ohmic or non-ohmic, the current of Bi2S3 nanowires was found to increase remarkably in H2 compared to that in a vacuum. Carrier density and mobility within the nanowires and the contact barriers between the nanowires and the electrodes have been extracted using field effect and two-probe current-voltage curves. It was found that H2 enhances electronic mobility and carrier density within the nanowires dramatically. The effect of H2 on the contact barriers was observed to be negligible compared to the other two effects.

Letter↗

The wrap-around problem and optimal padding in the exit wave reconstruction using HRTEM images.

A free fast Fourier transform software FFTW (www.fftw.org) is used in the exit wave reconstruction and shown to be able to handle images of any size effectively. The wrap-around problem commonly encountered in the exit wave reconstruction is investigated, and a new padding scheme is proposed and its performance is investigated. It is shown that while the usual wrap-around problem may be solved via padding the input high-resolution transmission electron microscopy (HRTEM) images with zero or averaged image intensities, the reconstructed exit wave at its boundary is inevitably distorted by the discontinuity of image intensities at the boundary. The proposed new padding scheme is shown to be able to provide a good estimate of the image intensities immediately outside the boundaries of the HRTEM images, reducing the estimated image intensity discontinuity across the boundaries and therefore providing a larger undistorted view of the exit wave.

Fourier Analysis↗

Prostaglandin E2 regulates Foxo activity via the Akt pathway: implications for pancreatic islet beta cell dysfunction.

AIMS/HYPOTHESIS: Prostaglandin E(2) (PGE(2)) is a well-recognised inhibitor of glucose-stimulated insulin secretion (GSIS). The aim of this study was to investigate the signalling pathway of PGE(2) in beta cell function regulation in HIT-T15 cells and isolated rat islets. MATERIALS AND METHODS: mRNA levels of the prostaglandin E receptor 3 (Ptger3) were measured by real-time PCR. Western blot analysis was used to detect changes in the levels of PTGER3, phosphorylated and total Akt, phosphorylated and total forkhead box 'Other' (Foxo). Transient transfection and reporter assays were used to measure Foxo transcriptional activity. The biological significance of PGE(2) in beta cell function was analysed using MTT, flow cytometry and GSIS assays. RESULTS: We found that treating HIT-T15 cells with exogenous PGE(2) stimulated Ptger3 gene expression specifically, and diminished cAMP generation. These were accompanied by the downregulation of Akt and Foxo phosphorylation in HIT-T15 cells and isolated rat islets. Moreover, PGE(2) upregulated basal and partially reversed constitutively active Akt-inactivated Foxo transcriptional activity. Furthermore, GSIS was impaired in PGE(2)-treated HIT-T15 cells and isolated islets. However, the dosage used in the above experiments did not affect beta cell viability and apoptosis. In addition, insulin-like growth factor 1 (IGF-1) pretreatment reversed the effects of PGE(2), and wortmannin treatment abolished the preventive effects of IGF-1. CONCLUSIONS/INTERPRETATION: Our observations strongly suggest that PGE(2) can induce pancreatic beta cell dysfunction through the induction of Ptger3 gene expression and inhibition of Akt/Foxo phosphorylation without impacting beta cell viability. These results shed light on the mechanisms of PGE(2) actions in pancreatic beta cell dysfunction.

Androstadienes↗

Fermented papaya preparation attenuates beta-amyloid precursor protein: beta-amyloid-mediated copper neurotoxicity in beta-amyloid precursor protein and beta-amyloid precursor protein Swedish mutation overexpressing SH-SY5Y cells.

Recent studies indicate that the deposition of beta-amyloid (Abeta) is related in the pathogenesis of Alzheimer's disease (AD), but the underlying mechanism is still not clear. The abnormal interactions of Abeta with metal ions such as copper are implicated in the process of Abeta deposition and oxidative stress in AD brains. In the present study, we established a new AD model, using which we found that copper triggered the Abeta neurotoxicity in SH-SY5Y cells overexpressing the Swedish mutant form of human APP (APPsw) in a concentration dependent manner. Fermented papaya preparation (FPP) has shown high free radical scavenging ability in vivo and in vitro. FPP post-treatment increased cell viability and decreased the intracellular [Ca2+]i, reactive oxygen species (ROS) generation such as hydroxyl free radical and superoxide anion and nitric oxide (NO) accumulation in the cell. Our results also show that FPP prevents the cell apoptosis through bax/bcl-2 sensitive pathway.

Amyloid beta-Peptides↗

Regulation of synaptic input to hypothalamic presympathetic neurons by GABA(B) receptors.

The hypothalamic paraventricular (PVN) neurons projecting to the spinal cord and brainstem play an important role in the control of homeostasis and the sympathetic nervous system. Although GABA(B) receptors are present in the PVN, their function in the control of synaptic inputs to PVN presympathetic neurons is not clear. Using retrograde tracing and whole-cell patch-clamp recordings in rat brain slices, we determined the role of presynaptic GABA(B) receptors in regulation of glutamatergic and GABAergic inputs to spinally projecting PVN neurons. The GABA(B) receptor agonist baclofen (1-50 microM) dose-dependently decreased the frequency but not the amplitude of spontaneous excitatory postsynaptic currents (sEPSCs) and inhibitory postsynaptic currents (sIPSCs). The effect of baclofen on sEPSCs and sIPSCs was completely blocked by 10 microM CGP52432, a selective GABA(B) receptor antagonist. Baclofen also significantly reduced the frequency of both miniature excitatory and miniature inhibitory postsynaptic currents (mEPSCs and mIPSCs). Furthermore, uncoupling pertussis toxin-sensitive G(i/o) proteins with N-ethylmaleimide abolished baclofen-induced inhibition of mEPSCs and mIPSCs. However, the inhibitory effect of baclofen on the frequency of mIPSCs and mEPSCs persisted in the presence of either Cd2+, a voltage-gated Ca2+ channel blocker, or 4-aminopyridine, a blocker of voltage-gated K+ channels. Our results suggest that activation of presynaptic GABA(B) receptors inhibits synaptic GABA and glutamate release to PVN presympathetic neurons. This presynaptic action of GABA(B) receptors is mediated by the N-ethylmaleimide-sensitive G(i/o) proteins, but independent of voltage-gated Ca2+ and K+ channels.

4-Aminopyridine↗

Quantitative analysis of defects and domain boundaries in mesoporous SBA-16 films.

Some quantitative structural analyses on defects and domain boundaries observed in SBA-16 films were performed using the lattice concept and geometric phase method. These analyses show that there exist low angle, high angle and translational anti-phase domain boundaries in SBA-16 films. While some of the domain boundaries bear analogue to those found in normal solid crystals, others are similar to that found in the liquid crystals. In particular near Sigma11 and Sigma13b high angle boundaries were observed. On the one hand the Sigma11 boundaries were found to exist with or without steps (ledge) associated with them depending on whether or not the boundary plane is parallel to the densely packed lattice plane. On the other hand segments of the boundary plane in the Sigma13b boundary were found being always associated with densely packed lattice plane, with the {011} type of lattice plane in one domain being parallel to the {112} type of plane in the other domain. The translational domain boundary was observed to have a translation vector having a projected component of (1/2) <110> on the (111) plane. The bending deformation similar to that found in the nematics liquid crystal was also observed and quantitatively analyzed using the geometric phase method, and rotational field associated with the deformation was identified.

Journal Article↗

High-quality ultralong Sb2Se3 and Sb2S3 nanoribbons on a large scale via a simple chemical route.

Large-scale ultralong single-crystalline Sb2Se3 and Sb2S3 nanoribbons were prepared respectively by reacting SbCl3 with selenium and sulfur powders in glycol solution. Both Sb2Se3 and Sb2S3 nanoribbons are usually hundreds of microns in length, and the structures of the nanoribbons are determined to be of the orthorhombic phases. The Sb2Se3 nanoribbons are typically 100-300 nm in width and 20-60 nm in thickness and grow along the [12] direction. Sb2S3 nanoribbons are wider than Sb2Se3 nanoribbons; Sb2S3 nanoribbons are about 200-500 nm in width and grow along the [001] direction. The growth mechanism of the nanoribbons is investigated based on high-resolution transmission electron microscopy (HRTEM) observations. Optical absorption experiment reveals that Sb2Se3 and Sb2S3 nanoribbons are two semiconductors with bandwidth Eg approximately 1.15 eV and Eg approximately 1.56 eV, respectively.

Journal Article↗

Subtype IV of the BK polyomavirus is prevalent in East Asia.

BK polyomavirus (BKV) is ubiquitous in human populations, infecting children asymptomatically and then persisting in the kidney. Using either serological or genotyping methods, BKV isolates have been classified into four subtypes (I-IV), with subtype I mainly detected in all countries studied so far. To elucidate the subtype of BKV prevalent in East Asia, we examined BKV-positive urine samples collected from immunocompetent elderly patients in Mongolia, Northeast China, Northwest China, Southeast China, Southwest China, Vietnam and Japan. The 287-bp typing region of the viral genome in each of these samples was PCR-amplified and sequenced, and a phylogenetic tree was constructed. According to the tree, BKV isolates in East Asia were unambiguously classified into subtype I or IV (subtypes II and III were not detected). In Japan, subtype I was mainly detected and subtype IV was rare, whereas in the other regions subtype IV was detected frequently, at rates ranging from 24 to 100%. Thus, East Asia (excluding Japan) is a region in which subtype-IV BKV is prevalent, a finding that requires the view of the geographic distribution of BKV subtypes to be revised. Furthermore, we present evidence that the immunological states of urine donors do not affect the pattern of BKV subtypes.

BK Virus↗

Growth of Agropyron elongatum in a simulated nickel contaminated soil with lime stabilization.

A greenhouse study was performed to evaluate the feasibility of using Agropyron elongatum and lime treatment for remediation of a simulated nickel (Ni) contaminated acidic soil. The acid soil was spiked with four levels of Ni, i.e., 0, 15, 50, and 100 mg Ni kg(-1) soil and the soil pH at each Ni level was adjusted to 4, 6 and 8 by liming with CaO. Addition of lime raised the soil pH and resulted in a decrease in concentrations of DTPA-extractable and water-soluble Ni in the soil, with a maximum reduction of 64 and 28 mg kg(-1), respectively at the highest level of Ni treatment as compared to the control without Ni treatment. In general, excessive Ni content in soil, especially at pH 4 inhibited the growth of Agropyron. Liming improved the growth condition and the highest dry weight yield of 12 g pot(-1) was obtained in soil spiked with 15 mg Ni kg(-1) at pH 6; and at the highest level of Ni, the dry weight yield increased from 0.48 to 5.96 g pot(-1) when the pH was increased from 4 to 6. However a further increase in pH to 8 did not improve plant yield because of the unavailability of nutrients at such a high pH. Nickel contents in plant were positively correlated to soil Ni contents at each respective pH level. Most of the Ni taken up by Agropyron was retained in the roots and the highest Ni uptake by plant was 3.9 mg pot(-1) in soil spiked with 100 mg Ni kg(-1) at pH 5.9, which was about 2.6% of total Ni added to the soil. This implies that Agropyron is not a hyperaccumulating species but is highly tolerant. Lime amendment together with the tolerant characteristic of Agropyron could provide a possible means to remediate Ni contaminated soils through phytostabilization.

Agropyron↗

Formation of hydrogen-bridged cytosine dimers on Cu(110).

Cytosine was adsorbed onto a Cu(110) surface under UHV conditions. Annealing to 370 K resulted in the formation of a (6 x 6)gg low energy electron diffraction (LEED) pattern, even at submonolayer coverages. Examination of this structure with scanning tunneling microscopy (STM) revealed islands of zigzag chains at low coverages and large ordered domains at monolayer saturation. Further annealing to 480 K initiated a phase transition to a (6 x 2)gg structure observed both by LEED and STM. High resolution electron energy loss spectroscopy spectra for both overlayer structures exhibited mainly in-plane modes suggesting upright/tilted species on the surface. Based on the experimental data and supported by density functional theory calculations, a model is proposed for the (6 x 2)gg structure, which involves the formation of deprotonated hydrogen bridge-bonded cytosine dimers, adsorbed through the oxygen atoms.

Adsorption↗

Application of a generic biosphere model for dose assessments to five European sites.

The BIOMOSA (BIOsphere MOdels for Safety Assessment of radioactive waste disposal) project was part of the EC fifth framework research programme. The main goal of this project was to improve the scientific basis for the application of biosphere models in the framework of long-term safety studies of radioactive waste disposal facilities and to enhance the confidence in using biosphere models for performance assessments. The study focused on the development and application of a generic biosphere tool BIOGEM (BIOsphere GEneric Model) using the IAEA BIOMASS reference biosphere methodology, and the comparison between BIOGEM and five site-specific biosphere models. The site-specific models and the generic model were applied to five typical locations in Europe, resulting in estimates of the annual effective individual doses to the critical groups and the ranking of the importance of the exposure pathways for each of the sites. Uncertainty in the results was also estimated by means of stochastic calculations based on variation of the site-specific parameter values. This paper describes the generic model and the deterministic and stochastic results obtained when it was applied to the five sites. Details of the site-specific models and the corresponding results are described in two companion papers. This paper also presents a comparison of the results between the generic model and site-specific models. In general, there was an acceptable agreement of the BIOGEM for both the deterministic and stochastic results with the results from the site-specific models.

Body Burden↗

Quantitative analysis of electron field-emission characteristics of individual carbon nanotubes: the importance of the tip structure.

Electron field-emission measurements on individual carbon nanotubes (CNTs) were performed inside the transmission electron microscope (TEM). The field-emission characteristics of CNTs with different tip structures were compared, and their field conversion factor and emission area were studied systematically. It was found that the field-emission characteristics of a CNT depend sensitively on its tip structure, and in particular an opened CNT was shown to be superior to a capped CNT. High-resolution TEM observations revealed that the tip of an opened CNT may, in general, be regarded as being composed of irregular shaped graphitic sheets, and these graphitic sheets have been found to improve dramatically the field-emission characteristics, but the sharp edge may result in larger error in the calculated emission area. The influence of uncertainty in the work function of the CNTs on the field conversion factor and emission area calculation was also investigated.

Journal Article↗

MPZL1/PZR, a novel candidate predisposing schizophrenia in Han Chinese.

The MPZL1/PZR gene has been mapped to 1q23.3, located in close proximity to a recognized schizophrenia susceptibility locus. Recently, the MPZL1/PZR gene has been found to be significantly upregulated in schizophrenia brain tissue and to play an important role in cell signaling, thus indicating that MPZL1/PZR could be a potential schizophrenia marker. To test this hypothesis, we selected three single nucleotide polymorphisms (SNPs) for genotyping in 523 Han Chinese trios. We found that two individual SNPs were significant at the Bonferroni's corrected significance level P<0.017: rs3767444 (chi2=6.299, P=0.0121) and rs2051656 (chi2=9.856, P=0.0017). Haplotype transmission/disequilibrium tests revealed a significant association with the disease (global P-value=1.064 x 10(-6)), but no specific transmission distortions. Thus, we propose that the MPZL1/PZR gene may be important in the predisposition to schizophrenia among Han Chinese.

Adult↗

Synthesis and characterizations of amorphous carbon nanotubes by pyrolysis of ferrocene confined within AAM templates.

Amorphous carbon nanotubes (a-CNTs) are synthesized by pyrolysis of ferrocene confined in the nanopores of the anodic alumina membrane (AAM) and characterized by field emission scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), electron energy-loss spectroscopy (EELS), and Raman spectroscopy. It is shown that the a-CNT has an ultrathin amorphous wall (approximately 3 nm) and a relatively large diameter (approximately 50 nm), and is capsulated with iron oxide nanoparticles. It is found that the growth of the a-CNTs is governed mainly by the template limitation effect. Electrical transport measurements on individual a-CNTs demonstrate that the a-CNT may be connected with electrodes via either ohmic or Schottky contacts, and the resisitivity of the a-CNTs was measured to be 4.5 x 10(-3) Omega cm.

Journal Article↗

In situ fabrication and graphitization of amorphous carbon nanowires and their electrical properties.

Individual amorphous carbon nanowires (a-CNWs) were fabricated inside a transmission electron microscope (TEM) by the electron beam induced deposition (EBID) method, and the a-CNWs were graphitized in situ by introducing Fe particles into these a-CNWs and controlled movement of the Fe particles in these CNWs. Detailed structural characterizations and electrical measurements were carried out, and it was found that the current-induced movement of Fe particles has significant effects in purifying the as-fabricated a-CNW, transforming the a-CNW into a graphitized-CNW (g-CNW). Two-terminal current voltage characteristics measurements showed that the g-CNW has a very good electrical conductivity with a resistivity of about 5.3 x 10(-4) Omega cm, a current carrying capacity of at least 4.35 mA, and a current density of 4.6 x 10(8) A/cm(2), and these values are comparable to those of multiwalled carbon nanotubes. Field emission characteristics of both a-CNWs and g-CNWs were also measured, and their respective Fowler-Nordheim plots were found to have basically a linear form.

Journal Article↗