Biomedical subjects
Publications and source records attributed to Q D Lin.
This paper describes the open permanent MRI technology, introduces the special technology such as main magnet field, gradient magnetic field coil, Transmitter/Receiver coils and so on, and points out that the installation and adjustment of the MRI are also very important for optimal operation and best image quality.
In the paper, we introduce the studies on some abnormal points in static magnetic field, find out the causes and mean while, give the methods of exclusion, thus the adjustment of MRI can be made perfectly.
Prenatal diagnosis of Duchenne's muscular dystrophy (DMD) have been carried out on 12 fetus at risk. The gene mutations have been identified by hybridization with cDNA probes and/or multiplex PCR. The fetus examined were 7 males and 3 females. Three of the male fetus inherited the same deleted mutations as the probands, and other 4 appeared normal. Among the 3 female fetus, one carried a deleted gene, two were considered normal. The diagnosis of the fetus were confirmed after birth or abortion. As the multiplex PCR can quickly detect about 98% of the deletions on the dystrophin gene, it is not only an idea method for screening the gene deletion but can also be applied to prenatal diagnosis immediately after the nature of the deletion have been identified among the probands. The strategy of prenatal diagnosis of DMD in our country was also discussed.
UNLABELLED: T-lymphocyte subsets of peripheral blood were examined by flow cytometry (FCM) in 20 cases of unexplained habitual abortion (UHA) before and after active immunization treatment, with 10 normal pregnant (NP) women and 20 normal nonpregnant (NNp) women as control. THE RESULTS: there was no difference in the percentage of CD3 cells between the UHA cases and NNP. Before immunization the percentage of CD8 cells was significantly lower (P < 0.001) and the ratio of CD4/CD8 was increased (P < 0.05) in UHA as compared with the NNP. However, after immunization, the percentage of CD8 cells greatly increased (P < 0.05) and CD4/CD8 ratio decreased significantly (P < 0.05) nearly to that of NP. These findings suggest that: (1) the increase of CD4/CD8 ratio due to the decreasing CD8 cells is the underlying immunomechanism of UHA; (2) immunization may induce the proliferation of CD8 cells and the lowering of CD4/CD8 ratio. Therefore, CD8 cells might play an important role in maintaining the balance of fetal-maternal immunological relationship; (3) the ratio of CD4/CD8 may served as parameter for observing the clinical efficacy of immunization therapy.
Two hundred and forty five patients with recurrent abortions were studied for autoantibodies in this paper. The total positive rate of autoantibodies was found to be 18.4% (45/245). The presence of antiphospholipid antibodies was in 33 (13.5%), antinuclear antibodies in 17 (6.9%) and anti-ENA in 7 (2.9%) patients. According to the clinical data, these 45 patients were classified into three types: (1) cases with antiphospholipid antibodies; (2) cases with anti-ENA (SLE); (3) cases with simple antinuclear antibodies. All the patients except SLE cases were treated with low-dose prednisone (5 mg/day) and aspirin (60-80 mg/day). The total pregnancy success rate was 80.0%. Excluding SLE cases, the success rate was up to 92.9%. The outcome of pregnancy was usually related to whether the autoantibodies especially LAC turned negative or not. Hemorheology and coagulative state in 19 patients with autoantibodies revealed hypercoagulative condition. It suggests that autoantibodies may cause intravascular coagulation leading to recurrent abortions.
One hundred and fourty-eight cases of habitual abortion were screened for etiologies. We found 40.5% (60/148) were unexplained. Among them, 34 cases were volunteers for receiving repeated active immunolization with low-dose of lymphocyte from their husbands or other donors, and 12 cases were taken as control. The successful pregnant rate was 86.4% (19/22) in treatment group which is higher significantly than in control (P < 0.05). meanwhile, used by microlymphocytotoxic antibody test and one-way mixed lymphocyte reaction and its inhibition test, the observations on immunologic state before and after vaccination were made in 19 cases. We found both the positive rate of microlymphocytotoxic antibody test and the relative suppressive rate of lymphocyte response were increased significantly post-vaccination than pre-vaccination. These results not only confirmed that active immunization was effective, but also suggested that habitual abortion may be associated with the lack of protective antibody and/or block antibody.
The uterine spiral arteries were studied by the immunohistochemical ABC method in 30 cases of Pregnancy Induced Hypertension (PIH) and 10 cases of normal pregnant women. Depositions of IgM and/or C3 on the vessel wall were significantly observed only in PIH cases (15 cases, 50.0%; P < 0.05). Depositions of IgG were seen in 5 cases of PIH 16.7% showing significantly decrease. (P < 0.001), as compared with the normal pregnant women (9 cases, 90.0%). The results suggest that PIH is related to chronic rejection reaction resulting possibly from the decrease of local immunosuppression in pregnancy. Meanwhile the positive stain of IgG, IgM and C3 seen in the nuclei of decidual cells in some of PIH cases were discussed.
T-lymphocyte subpopulations were determined by the monoclonal antibodies (OKT3, OKT4, OKT8) in 20 cases of normal pregnancy, 36 cases of pregnancy-induced hypertension (PIH) and 20 cases of normal non-pregnant women. The assaying of ConA-induced suppressor T cell (Ts cell) function was carried out in another 20 cases of late pregnancy, 20 cases of moderate and severe PIH, and 10 cases of normal non-pregnant women. The results showed: the percentage of Ts cell increased significantly and the ratios of Th/Ts decreased significantly during normal pregnancy as compared with normal non-pregnant women. As compared with the late pregnant women, a lowering of Ts cell and elevating ratio of Th/Ts were found in PIH cases with statistical significance in severe PIH. A decrease of Ts cell function was also seen in moderate and severe PIH cases. All these findings suggest that the changes of Ts cell in amount and function may play an important role in maintaining normal pregnancy and in the pathogenesis of PIH.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.