PubMed Health⌕ Search

Biomedical subjects

Q Gou

Publications and source records attributed to Q Gou.

26 records · Page 2Linked to original sources
PubMed1993

A study of the genetic polymorphism of human inter-alpha-trypsin-inhibitor (ITI) in the Han population, Chengdu, China.

Phenotypes of inter-alpha-trypsin-inhibitor (ITI) have been determined by isoelectric focusing on polyacrylamide gels followed by immunofixation. The phenotype frequencies of ITI in the Han population in Chengdu, P.R. China have been investigated using this method. In addition, family studies have been conducted in 21 families. The results show that ITI is polymorphic in the Han population in Chengdu, China. The allele frequencies are as follows: ITI*1 = 0.5763. ITI*2 = 0.4107, ITI*3 = 0.0130. ITI is thus a new and promising genetic marker that can be used in the field of forensic haematogenetics.

Alpha-Globulins↗
PubMed1993

[Isolation and purification of alpha -1 acid glycoprotein from human sera].

Alpha-1 acid glycoprotein, also named orosomucoid (ORM), is a serum protein with genetic polymorphism. This paper describes a two-step procedure for purifying ORM from human sera. The procedure consists of both anion exchange chromatography on a DEAE-Sephadex-A50 column and affinity chromatography on a Reactive Blue 2-Sepharose CL-6B column. The purified ORM identified by polyacrylamide gel electrophoresis, SDS-polyacrylamide gradient gel electrophoresis, immunoelectrophoresis and immunodiffusion was homogeneous and reacted specifically with a commercial anti-ORM serum (Sigma). The molecular weight of the purified ORM was about 41 kd.

Electrophoresis, Polyacrylamide Gel↗
PubMed1992

Genetic polymorphisms of alpha-2-HS-glycoprotein, group-specific component and orosomucoid in the Han population, Chengdu, China.

The Han population in Chengdu, China, was investigated for genetic polymorphisms of alpha-2-HS-glycoprotein (A2HS), group-specific component (GC) and orosomucoid (ORM) using isoelectric focusing followed by immunofixation. The allele frequencies were: A2HS*1 = 0.6958,A2HS*2 = 0.3042, GC*1F = 0.4021, GC*1S = 0.3182, GC*2 = 0.2745, GC*1A = 0.0052, ORM1*F1 = 0.7028, ORM1*S = 0.2762, ORM1*F2 = 0.0210, ORM2*A = 0.9965, ORM2*Var = 0.0035.

Alleles↗
PubMed1992

[Detection of G2m(n) factor in bloodstains using ELISA inhibition test].

For the purpose of detecting G2m(n) factor in human bloodstains, an ELISA inhibition test using mouse antihuman G2m(n) monoclonal antibody was established. The results revealed that the correctness rate for G2m(n) factor detection was 100%. The minimal amount of bloodstain required for detecting G2m(n) factor was 0.25 cm x 0.125 cm. This approach provided a new method for individual identification of human bloodstains.

Antibodies, Monoclonal↗
PubMed1991

[Genetic polymorphism of alpha 2HS-glycoprotein in the Han population in Chengdu].

The distribution of alpha 2HS-glycoprotein (AHSG) phenotype frequencies in the Han population in Chengdu was studied using polyacrylamide gel isoelectric focusing followed by immunofixation with rabbit anti-human AHSG serum. Two hundred eighty-six serum samples collected at random from unrelated individuals were phenotyped for AHSG. The distribution of AHSG phenotype frequencies was found to be AHSG 1 = 47.20%, AHSG 2 = 8.04% and AHSG 2 - 1 = 44.76%. The observed numbers agreed well with the expected numbers calculated on the basis of the Hardy-Weinberg equilibrium. The allele frequencies were estimated to be AHSG.1 = 0.6958 and AHSG.2 = 0.3042. The discrimination probability of AHSG is 0.5704 and the exclusion probability of parentage 0.1669.

China↗