Biomedical subjects

Q Wang

Publications and source records attributed to Q Wang.

At least 235 records · Page 13Linked to original sources

PubMed2001

Eudesmanolides, aromatic derivatives, and other constituents from Carpesium cernuum.

A new eudesmanolide 13-hydroxy-4 alpha H-eudesman-5,7(11)-dien-12,8 beta-olide (1) and a new aromatic derivative 3-methyl-8-acetoxy-9,10-diisobutanoyloxy-p-cymene (6), along with ten known compounds were isolated from the roots of Carpesium cernuum L. Their structures were elucidated by spectral methods (IR, EIMS, FAB-MS, 1D and 2D NMR). Compound 2, 3 and compound 10 exhibited moderate antibacterial activity against Bacillus subtilis, Escherichia coli and Staphylococcus aureus.

Anti-Bacterial Agents↗

PubMed2001

[Genescan analysis of the loss of heterozygosity on the long arm of chromosome 6 in non-small cell lung cancer].

To investigate if there are microsatellite loci in the long arm of chromosome 6 that have close relationship with non-small cell lung cancer, Multiple PCR approach was used to analyze the 36 loci in the long arm of chromosome 6. The PCR products were analyzed in PAGE and then the electrophoresis maps were analyzed with Genescan and Genotyper. There is different LOH frequency in different loci. The total frequency of LOH in 41 lung cancers was 78%(32/41), with the highest frequency of LOH was detected on the locus D6S302(75%). There are 14 loci which have LOH frequency more than 20% and the loci are mainly located in 2 regions: 6q16.3-q21 [6 loci D6S458 (21.43%), D6S1694 (26.92%), D6S1717 (35.71%), D6S1565 (40%), D6S302 (75%), D6S1706 (36.36%) and 6q26-q27 (5 loci D6S1550 (38.46%), D6S264 (20%), D6S1585 (25%), D6S446 (33.33%), D6S281 (30.77%)], There may be tumor suppressor genes located in the two regions, which have a close relationship with non-small cell lung cancer.

Carcinoma, Non-Small-Cell Lung↗

PubMed2001

[Correction of occipito-posterior by maternal postures during the process of labor].

OBJECTIVE: To investigate the effect on correction of occipito-posterior (OP) by changing maternal posture during labor. METHODS: One hundred normal primigravida with head OP position in the latent phase of labor were randomly divided into 2 groups: Group A (n = 50), women were instructed to lay on the same lateral posture with the fetal spine during labor in order to correct the fetal position from OP to occipito anterior (OA); Group B (n = 50) lay on the opposite side to the fetal spine. The OP position was diagnosed by vaginal examination or B ultrasound, and the course of labor and mode of delivery were observed. RESULTS: Thirty-four women delivered vaginally (68%) in group A, with 27 of them turned to OA position (54%); spontaneously while they were 22 (44%) and 12 (24%) in group B respectively, a significant difference was shown (P < 0.005). The average time interval for the 1st stage was (13.5 +/- 6.5) hour and (17.1 +/- 7.2) hour for group A and B respectively, also a significant difference was noted (P < 0.01). CONCLUSION: To instruct women in labor to take the lateral recumbent position with the same side of fetal spine for correcting OP to OA is an effective method. It may increase vaginal deliveries and shorten the first stage of labor, thus reduce dystocia due to OP position. This method is simple and effective, and maybe adopted in most obstetric units.

Adult↗

PubMed2001

[Variation of soil microsite moisture-physical properties and its effect on Fraxinus mandshurica juvenile stand growth].

Soil microsite plays an important role in the regeneration, seedling survival, and individual growth of trees. This paper studied the relationship between soil microsite moisture-physical properties and Fraxinus mandshurica growth. Variation was found not only among different sites in traditional scale, but also among different microsites. Some variations of soil properties among different microsite were greater than those among different sites. The growth of Fraxinus mandshurica varied greatly among different microsites. Microsites affected most soil moisture-physical properties and tree growth. The study demonstrated the importance of matching trees to the proper microsite on the growth of plantation stand.

Oleaceae↗

PubMed2001

[Immunological regulations of dendritic cell in abnormal scarring tissue].

OBJECTIVE: To investigate the relationship between dendritic cell(DC) and pathogenesis of abnormal scar. METHODS: The content of HLA-DR and CD1a molecules of DC, in 6 samples of hypertrophic scar (HS), keloid (K) and normal skin, were determined with the stain of avidin-biotin-peroxidase complex method (ABC). The effect of Triamcinolone Acetonide was evaluated with the measurement of the HLA-DR and CD1a molecules of DC in the epidermis of the HS. RESULTS: 1. The amounts of HLA-DR molecules of the positive DC were 806.67 +/- 101.72 and 870.00 +/- 134.24 in the HS and the K respectively, significantly higher than the controlled normal skin (510.01 +/- 45.17, P < 0.05). HLA-DR molecules showed an abnormal expression in the Kerationcytes and fibroblasts. 2. The amounts of CD1a molecules of the positive DC were 700.00 +/- 97.23 and 780.00 +/- 104.47 in the HS and the K respectively, significantly higher than the controlled normal skin (521.24 +/- 57.87)(P < 0.05). 3. The amounts of the HLA-DR molecules positive DC, in the positive kerationcytes and fibroblasts of hypertrophic scar, treated by Triamcinolone Acetonide, were 476.67 +/- 70.02 and 447.76 +/- 90.03 (P < 0.05) for 3 days and 7 days treatment respectively, significantly lower than the control. The amounts of CD1a molecules positive DC in the epidermis of hypertrophic scar with the injection of Triamcinolone Acetonide were significantly lower in 3 days and 7 days treatment (456.36 +/- 82.88 and 397.18 +/- 99.36, P < 0.05). CONCLUSION: 1. The results, with the high expression of HLA-DR and CD1a molecules, indicate that the HS and the K may have strong immune reactions. 2. Triamcinolone Acetonide may decrease the immune reactions of the HS, through the inhibition of the expressions of HLA-DR and CD1a molecules in the dendritric cell.

Cicatrix, Hypertrophic↗

PubMed2001

[Composting process of municipal solid waste with high effective complex microbial community].

The effects of high effective complex microbial community (MECMC) in a combination composting process of municipal solid waste (MSW) and sludge were examined through inspecting biomass, temperature, organic matter, the carbon, nitrogen ration (C/N). Composting was performed under such conditions: The ratio of MSW:sewage:sawdust:composting of 45:25:15:15 (W/W) and organic matter of 60%, total N of 1.4%, total P of 0.69%, total K of 1.25%, original moisture of 58.5%, original ratio of C/N of 30, aration flow of 0.8 L/min.kg(volatile organic matter). The rates of adding HECMC to treatment 1, treatment 2 and treatment 3 were 2%, 3%, 5% respectively. Control experiment added 3% inactive HECMC. The day of MSW and sludge mature in control experiment, treatment 1, treatment 2 and treatment 3 were 30, 24, 18 and 12, respectivly. This indicated HECMC were effective to compose organic matter and speedup composting change into humus. There are many active bacteria in composting which are favorable for soil.

Bacteria↗

PubMed2001

[The effect of mouthguard on strength of the musculus deltoideus].

OBJECTIVE: To investigate the effect of mouthguard on strength of musculus deltoideus and to observe the possibility of its improving sports ability. METHODS: The custom mouthguards were fabricated respectively for 8 subjects with soft resin by direct method. The activities of the musculus deltoideus and masseter were tested by means of electromyograph (EMG), while the subjects lay, made maximum voluntary biting with mouthguard and pressed 5 kg. The EMG potentials were transferred to a computer. The data were handled and the integral values of EMG were exported. RESULTS: The subjects set a great value on the mouthguard's comfort, retention and protection. The EMG values of the musculus deltoideus and masseter of the subjects biting with mouthguards were 1.1600 v/s and 0.3825 v/s, and the values of those biting without mouthguards were 0.6125 v/s and 0.213 8 v/s, respectively. The muscular strength of the former was significantly improved compared with that of the latter (P < 0.01). CONCLUSIONS: Wearing a mouthguard and maximum voluntary biting can enhance the masseter force and reflectively improve the musculus deltoideus strength.

Electromyography↗

PubMed2001

[A case-control study on the risk factors of stomach cancer in Tianjin city].

OBJECTIVE: To explore the risk factors of stomach cancer in Tianjin. METHODS: A matched case-controls study was carried out. A total number of 189 patients with stomach cancer who were individually matched and interviewed. RESULTS: Multivariate conditional logistic analysis showed that stomach cancer was closely relate to the four factors: smoked food (OR = 2.34, 95% CI: 1.60-4.98), cigarettes smoking (OR = 6.07, 95% CI: 1.26-7.16), heavy salt intake (OR = 1.95, 95% CI: 1.27-3.23) and excessive intake of meats (OR = 1.46, 95% CI: 1.05-2.02) were risk factors for stomach cancer. CONCLUSION: Frequent eating smoked food, cigarettes smoking, heavy salt intake and over intake of meats were risk factors for stomach cancer.

Adult↗

PubMed2001

[Analysis of loss of heterozygosity on 19p in primary gastric cancer].

OBJECTIVE: To investigate the loss of heterozygosity (LOH) frequency of microsatellite loci in primary gastric cancer samples and locate the deleted regions on 19p in which might exist human gastric cancer related genes. METHODS: The LOH of microsatellite loci on chromosome 19p was analyzed using PCR-SSLP-silver stain method in 43 primary gastric cancers and their paired normal tissues. RESULTS: In 43 primary gastric tumors, LOH was detected on the site for D19S424(29.63%), D19S216(11.53%), D19S406 (33.33%), D19S413(8.57%), D19S221(13.15%), D19S226(8.00%), D19S411(6.45%), D19S883(6.89%), and D19S886(10.71%), microsatellite instability (MSI) was found at the same time at locus D19S886 (17.85%). CONCLUSION: The most common LOH occurrence at D19S406 and D19S424 might imply the existence of the potential genes related to the tumorigenesis of gastric cancer in these loci.

Chromosome Mapping↗

PubMed2001

[Docitaxol-induction apoptosis of osteosarcoma].

OBJECTIVE: To study the growth inhibition and apoptosis induction effect of docitaxol on osteosarcoma cells. METHODS: The effect of docitaxol on osteosarcomat cell line PL-1 was examined by cell count, morphologic observation, flow cytometry (FCM) and electronmicroscopy. RESULTS: Docitaxol inhibited the growth of PL-1 in a time and dose-dependent manner. The osteosarcoma cells were blocked at G1 phase of the cell cycle. The treated cells presented features typical of apoptosis. Cells recultured shortly after being treated with docitaxol were more apt to undergo apoptosis than cells treated with docitaxol continuously. CONCLUSION: The growth inhibiting effect of docitaxol is due to its ability to induce apoptosis. It shows great potential in the treatment of osteosarcoma.

Antineoplastic Agents, Phytogenic↗

PubMed2001

[Video colposcopy in diagnosis of cervical squamous intraepithelial lesions].

OBJECTIVE: To study the accuracy and efficiency of video colposcopy in diagnosis of cervical squamous intraepithelial lesions and clinical significance of seven colposcopic signs. METHODS: Two hundred and seventy-five patients with an abnormal cervical smear or vulva condyloma acuminata were re-evaluated by video colposcopy. 265 cervical biopsies performed under the guidance of video colposcopy for pathological study, as well 8 endocervical curettage (ECC) and 237 loop electrosurgical excision procedure (LEEP). RESULTS: The sensitivity, specificity and positive rate of video colposcopy in evaluation of cervical squamous intraepithelial lesions were 95.9%, 77.7%, and 84.5%. Video colposcopy diagnostic accuracy was 93.8% tally with the pathological results in observing normal transformation zone. False negative rate was 6.1%. 76.2% of cases with subclinical papillomavirus infection by acetowhite epithelium, while false negative rate was 23.7%. Twin signs, triple signs and cervical carcinoma were 100.0% tallying with the diagnosis of cervical lesions. CONCLUSIONS: Video colposcopy can be a very accurate diagnostic method in clinical practice. It is quite sensitive in diagnosis of severe cervical lesions. However, there was a relative high false negative rate in diagnosing mild cervical lesions.

Colposcopy↗

PubMed2001

Cyclosporin A treatment for idiopathic membranous nephropathy.

OBJECTIVE: To evaluate the efficacy of cyclosporin A (CSA) in the treatment of idiopathic membranous nephropathy (IMN), a prospective controlled clinical study was performed. METHODS: This study included a group of 30 IMN patients, among them 15 were treated with CSA and 15 with captopril (CAP). The diagnosis of IMN was made with exclusion of secondary forms of membranous nephropathy by extensive clinical and pathological studies. No patients received steroids or cytotoxic agents for six months prior to enrollment. In the CSA group, CSA was given at an initial dosage of 5 mg.kg-1.d-1, gradually tailed off over the first three months and maintained at 2 mg.kg-1.d-1 for 12 months. In the CAP group, CAP was given at a dosage of 37.5 mg/day. RESULTS: In the first three months, 6 (6/15) complete remissions (CR) and 2 (2/15) partial remissions (PR) were observed in the CSA group while only 2 (2/15) PRs were observed in the CAP group. Before the end of the 15-months, 8 patients in the CSA group experienced CR and 4 patients experienced PR. One CR patient relapsed as the dosage of CSA was reduced, so 7 patients remained in CR at the end of the first 15-months. No additional CR or PR was observed in the CAP group during late follow-up. At the last visit (an average follow-up time of 44 months) in the CSA-group, another 2 CR patients had relapsed and 1 CR patient shifted to PR after stopping the CSA treatment, so 4 CR and 5 PR remained in the CSA group. In the CAP group, 3 spontaneous CRs occurred beyond 1.5 year's follow-up, with 3 CR and 2 PR at the last visit. No difference was found between the averages of the initial and the last serum creatinine levels in either group. No serious adverse effects were found during CSA treatment. Re-biopsy data of three patients responsive to CSA treatment showed that no pathological improvement of glomerular basement membrane was observed, even in cases at remission. Tubulointerstitial fibrosis was found in 1 relapsed CR patient, whose serum creatinine increased above the normal range, but not in the other 2 patients whose serum creatinine remained in the normal range. CONCLUSIONS: CSA therapy at a dosage of 5 mg.kg-1.d-1 is effective in inducing remission of nephrotic syndrome in adult IMN patients within three months, with a response rate of 80%. A relatively high rate of relapse (50%) was observed within 2 years after the withdrawal of CSA treatment.

Adult↗

PubMed2001

Contrast enhanced MR angiography in pulmonary sequestration.

OBJECTIVE: To evaluate the feasibility of contrast enhanced three-dimensional (3D) magnetic resonance angiography (MRA) in identifying the systemic blood supply in pulmonary sequestration. METHODS: Three patients who were suspected of pulmonary sequestration were examined by contrast enhanced 3D MRA. MR images were compared with surgical findings. RESULTS: Contrast enhanced 3D MRA clearly demonstrated systemic arteries from the descending thoracic aorta supplying the basilar segments of the lower lobe in each case. Pulmonary veins from these segments, which drained into the left atrium in two cases, were also detected. MRA images were consistent with those observed in surgery. CONCLUSION: Contrast enhanced 3D MRA allows accurate diagnosis of pulmonary sequestration and may obviate the need for more invasive investigations.

Adult↗

PubMed2001

[An epidemiological and clinical study of primary gout].

OBJECTIVE: To evaluate the prevalence of primary gout in Shantou and Beijing area, and understand it's clinical features. METHODS: Three epidemiological studies in Chenghai downtown, and the one of study in Beijing were studied. Clinical, laboratory and radiology data were collected and analyzed. RESULT: The prevalence of primary gout in Chenghai surveys was 0.17%, 0.15% and 0.26% respectively; no case of primary gout was found in Beijing survey. Among the patients, male to female ratio was 21:1; average onset age of females was significantly higher than that of males. The numbers of patient in the recent 10 years was increasing. At the first attack, the first metatarsophalangeal joint was involved among 79.7% of all cases; no fourth metatarsophalangeal joint involvement had been found. Hyperlipemia was the most common accompanying abnormal laboratory test (57.1%). Radiographic bone erosion was found in 49.4% patients, most of them, the duration of disease was over 5 years. CONCLUSION: The prevalence of primary gout was higher in Shantou area than that in Beijing, and it was increasing in the recent 10 years in Shantou area. The difference of geography, climate, food and lifestyle could be responsible for the different prevalence in different area.

Adult↗

PubMed2001

[Renal protective effects of vitamin E in diabetic rats].

OBJECTIVE: To investigate the renal protective effect of vitamin E on streptozotocin induced diabetic rats, as well as the possible mechanism involved in. METHODS: Vitamin E 20 mg x kg(-1) x d(-1) was given to diabetic rats for 8 weeks. Urinary albumin excretion (UAE) was measured by radioimmunoassay. The changes of creatinine clearance rate (Ccr), nitric oxide(NO), nitric oxide synthase (NOS) and endothelin(ET) in the plasma and renal cortical tissue, membrane protein kinase C(PKC) in the renal glomeruli were observed. RESULTS: At two weeks, diabetic rats had supernormal Ccr[(6.47 +/- 1.51) ml x min(-1) x kg(-1)], UAE[(15.60 +/- 1.64) microg/24h], NO[(37.30 +/- 3.77) micromol/L], NOS[(34.89 +/- 3.83) U/L] and PKC[(86.85 +/- 11.37) pmol x min(-1) x mg protein(-1)] of diabetic rats were increase but ET was decreased. At 8 weeks, PKC[(84.18 +/- 12.14 ) pmol x min(-1) x mg protein(-1)] and ET of diabetic rats was increased significantly, but NO[(22.75 +/- 2.89) micromol/L] and NOS[(21.34 +/- 1.92) U/L] of diabetic rats was lowered. After treated with vitamin E, Ccr[(4.46 +/- 0.49) ml x min(-1) x kg(-1)], UAE[(16.31 +/- 1.12) microg/24h], PKC[(65.19 +/- 8.83) pmol x min(-1) x mg protein(-1)] at 8 weeks were decreased significantly. Both NO[(33.13 +/- 3.77) micromol/L] and NOS[(30.16 +/- 2.89) U/L] in plasma and kidney tissue were lowered as compared with untreated diabetic group in two weeks. Vitamin E also delayed the NO and NOS lowering in 8 weeks. CONCLUSION: Vitamin E could ameliorate early diabetic renal hemodynamic abnormality via inhibiting PKC, in which inhibition of NO production might be involved. It is important to reduced the PKC activity so as to prevent or delay the development of diabetic nephropathy.

Animals↗

PubMed2001

[A study on the use of traditional Chinese medicine agent in ultrasonic instrumentation of root canals].

OBJECTIVE: To evaluate the bactericidal effect for anaerobes of infected root canals using Chinese medicine agent Jieeryin combing with ultrasonic technique. METHODS: 60 single-rooted teeth in patients with chronic periapical periodotitis were divided into 3 groups randomly, 20 in each. Hand instruments were used for group 1 as control group of traditional treatment. Ultrasound with saline as irrigant were used for group 2 as control group. Ultrasound with Jieeryin as irrigant were used for group 3 as experimental group. The pre-instrumentation and post-instrumentation specimens of each root canal were placed into the anaerobic chambers and incubated. RESULTS: Significant difference was found between hand instruments group and control group of ultrasound (P < 0.05) and even more significant difference was found between control group of ultrasound and experimental group of ultrasound (P < 0.01). CONCLUSIONS: Ultrasound has special superiority on instrumentation and bactericide of root canals. Chinese medicine agent Jieeryin can kill anaerobes in infected root canals effectively. It is an ideal irrigant for ultrasound which conforms to principles of biology.

Adolescent↗

PubMed2001

[Soil P availability in larch rhizosphere].

Soil samples were collected from the rhizosphere and non-rhizosphere of larch(Larix gmelini) stand by exfoliation method, and the variation of P concentration was analyzed. The results show that there was no significant difference between total P concentrations in the rhizosphere and the non-rhizosphere soil, while the concentration of available P in rizhosphere soil was significantly higher than that in the non-rhizosphere soil. The concentrations of available P in rhizosphere 12 years old and 40 years old larch rhizosphered by 12.6% and 23.4% respectively, indicating that larch roots had the effect of mobilizing soil phosphorus. The components of inorganic P in rhizospheric soil were different from those in non-rhizospheric soil. The concentration of O-P was lower, and the concentrations of Al-P, Fe-P, Ca-P, NH4Cl-P were higher in the rhizosphere. The pH of rhizospheric soil was lower than that of non-rhizospheric soil, but no further acidification was observed. There was no close relationship between pH variation and available P concentration in larch rhizosphere.

Hydrogen-Ion Concentration↗

PubMed2001

Allogeneic bone nail for articular fractures.

OBJECTIVE: To investigate the effect of allogeneic bone nail on articular fractures. METHODS: Ninety-one patients with cancellous bone fracture involved in various joints were fixed with the bone nail or the bone screw. No immunosuppressive agent was used in this series. The average period of follow-up wa s 18 months. RESULTS: All patients except one healed without displacement an d the healing time was within 1-3 months. Joint function was partially limited in 6 patients and others were functioning well. CONCLUSIONS: Allogeneic bone nail possesses a good biological compatibility and intensity, thus provides a rigid fixation. Being completely absorbed and ossificated, thus obviating the need for secondary operation, is a suitable internal fixation material for the treatment of articular fractures.

Acetabulum↗