Biomedical subjects

Qi-wei Zhang

Publications and source records attributed to Qi-wei Zhang.

13 recordsLinked to original sources

PubMed2006

[Study on quality control of Paeonia lactiflora].

OBJECTIVE: To provide the experimental data for the quality control of Paeonia lactiflora a prepared Chinese medicinal herb. METHOD: The contents paeoniflorin among different growing areas and different processing methods methods were assayed by HPLC. RESULT: The quantitative differences of paeoniflorin content among different growing areas and different processing methods wereanalyzed quantitatively. CONCLUSION: The quantitative differences among different growing areas were not significant and the quantitative differences among different processing methods within 10%.

Benzoates↗

PubMed2006

[Experimental study on processing of Paeonia lactiflora].

OBJECTIVE: To provide experimental data for the quality control of processed Paeonia lactiflora, a Chinese herbal medicine. METHOD: Traditional processing of P. lactiflora was simulated, content of paeoniflorin and water extracts among different preparations were assayed by HPLC; The quantitative correlations among different processing conditions were analyzed, the effects of processing parameters on the contents of paeoniflorin and water extracts were assayed a nanalysed. RESULT AND CONCLUSION: The controlled processing parameters were correlated with covariables which showed that processing procedures was controllable, and the heating temperature was a factor impacting the content of paeoniflorin.

Benzoates↗

PubMed2006

[Study on the cholelitholytic effect and safety of the preparation of Chinese medicine compound].

OBJECTIVE: To study the cholelitholytic effect and safety of the preparation of Chinese medicine compound. METHOD: Experiments were performed to study the efficacy of gallstones dissolution of the preparation of Chinese medicine compound in vitro and in vivo. The toxicity was studied by pathological and blood biochemical changes. RESULT: The preparation of Chinese medicine compound dissolved cholesterol and mixing gallstones in 7 days in vitro and in vivo, no influence on the blood biochemistry and the other organs of the rabbits. CONCLUSION: The preparation of Chinese medicine compound is effective and safe for treatment of chololithiasis.

Animals↗

PubMed2006

[Determination of akebia saponin D in root of Dipacus asperoides by HPLC].

OBJECTIVE: To develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides. METHOD: An IPLC analytical method was established using Kromasil ODS column with acetonitrile-water (30:70) as mobile phase and the detection wavelength was 212 nm. RESULT: The akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 microg, r = 0.9999. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively. CONCLUSION: This method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations.

China↗

PubMed2005

[Quantitative fluorogenic real-time PCR assay for respiratory syncytial virus detection].

OBJECTIVE: To Establish a rapid and objective quantitative fluorogenic real-time PCR assay for early detection of human respiratory syncytial virus (hRSV). METHODS: Two pairs of primers and one TaqMan Fluorogenic probe that are specific for the recognition of the most conservative N gene of hRSV for virus detection with LighCycler PCR in 93 nasopharyngeal secretion specimens collected from infants and young children. The assay was compared with virus isolation, routine PCR, nested PCR, and enzyme-linked immunosorbent assay (ELISA). RESULTS: This TaqMan assay had a sensitivity of 1 x 10(2) cDNA copies/microl with a dynamic range between 1 x 10(2) and 1 x 10(7) cDNA copies/microl, which was the same as that of nested PCR, but 10 times more sensitive than routine PCR. The specificity of the assay was evaluated by comparing hRSV with polivirus type 1, coxsackie virus type 2, influenza A, influenza B and adenovirus type 7. A PCR product of the expected size (195 bp) was produced and fluorescence signal detected for hRSV, but not for any of the other viruses. The results in LightCycler and Rotor-Gene instrument were consistent. Forty-four specimens (43.9%) were hRSV-positive with this assay and 4 (4/93,4.3%) were hRSV-positive with ELISA, showing rather low correlation between the two methods. No visible relation was found between the concentration of hRSV RNA and severity of the disease. CONCLUSION: This assay is rapid, sensitive, specific and quantitative, and has the potential of wide application for early diagnosis of hRSV infection and evaluation of the therapeutic effect.

Child, Preschool↗

PubMed2004

[Study on applying chromatographic fingerprints to establishing quality criterion of processed tuber of Pinellia pedatisecta].

OBJECTIVE: To explore the utility of principal component analysis (PCA) in conjunction with the window preprocessing scheme on chromatographic data for quality control. METHOD: Chromatographic fingerprints of processed tuber of Pinellia pedatisecta were determined by HPLC, and the Window Preprocessing and PCA were used for data processing. RESULT: The quantitative differences among different growing areas and different process batches were found with this method. CONCLUSION: The method can be used in quality control for monitoring between-batch products of traditional Chinese pharmaceutical process.

China↗

PubMed2004

[Study on traditional Chinese pharmaceutical processing of radix Pinellia pedatisecta].

OBJECTIVE: To study the effects of concentration, volume and temperature of aluminium water on the change of chromatoghraphic data of processed Rhizoina Arisaematis. METHOD: Chromatographic fingerprints of processed Radix Pinellia pedatisecta were determined by HPLC, and multivariate analysis was made for data processing. RESULT AND CONCLUSION: No change on the whole chromatoghraphic data was observed at different levels of concentrition and volume of aluminium water, while there was a great change at different levels of temperature.

Alum Compounds↗

PubMed2004

[Studies on chemical constituents in bud of Artemisia scoparia (III)].

OBJECTIVE: To investigate the chemical constituents in flower bud of Artemisia scoparia. METHOD: The methanol extract was isolated by column chromatography with silica gel, polyamide and Sephadex LH-20 as packing materials and HPLC, six compounds were obtained and their structures were identified by UV, NMR and MS. RESULT: Six compounds were identified as beta-sitosterol (IX), 6,7-dimethoxycoumarin (X), 6-demethylcapillarisin (XI), capillartemisin B (XII), kumatakenin (XIII), isoscoploletin-beta-D-glucoside (XIV). CONCLUSION: XI, XII, XIII and XIV were obtained from the plant for the first time, and XI is a new compound.

Artemisia↗

PubMed2004

[Studies on chemical constituents in bud of Artemisia scoparia (II)].

OBJECTIVE: To investigate the chemical constituents in flower bud of Artemisia scoparia. METHOD: The constituents were isolated and purified by means of various chromatographic methods, and spectroscopic methods were used to identify their structures. RESULT: Four flavones were isolated and their structures were identified as hyperin (V), eupafolin (VI), pedalitin (VII), 5,7,2',4'-tetrahydroxy-6,5'-dimethoxyflavone (VIII). CONCLUSION: Compounds V, VI, VII, VIII were obtained from this plant for the first time.

Artemisia↗

PubMed2003

[Study on extraction conditions for minute powder of processed rhizome of Corydalis with vinegar].

OBJECTIVE: To examine the factors in extracting the minute powder of processed rhizome of Corydalis with vinegar for its application. METHOD: The one-way variation analysis was used for experimental design. Tetrahydropalmatine was determined with HPLC method. The samples were separated on a Diamonsil C18 column with mobile phase of acetonitrile-TEA phosphate buffer (pH 7.0) (50:50) and detection wavelength was 280 mm. RESULT: The extraction conditions were established as follows: extraction temperature is 60 degrees C, water-added amount is 20 times the weight of the powder, and extraction time is 30 minutes. CONCLUSION: The extraction rate of the tetrahydropalmatine in the minute power is about 10% higher than that of the processed rhizome with vinegar. But less water amount, less time, and lower temperature were needed for extraction. The application of minute powder can reduce energy consumption and raise economic benefit in industrial production.

Acetic Acid↗

PubMed2002

[Determination of cirsilineol in Herba Artemisiae scopariae collected in autumn by HPLC].

OBJECTIVE: To establish a quantitative method for determination of cirsilineol in Herba Artemisiae Scopariae collected in Autumn. METHOD: Sample was extracted with methanol, and cleaned up with polyamide column. ODS column was used with methanol-acetonitrile-0.5% acetic acid solution (24:19:57) as mobile phase. Detection wavelength was 347 nm. RESULT: Cirsilineol in sample solution was well separated. Linearity of cirsilineol was good (r = 0.9998) in range of 0.1-0.8 microgram. The average recovery was 101.3%, RSD of repeatability was 3.04%. CONCLUSION: This method can be used for quality control of Herba Artemisiae Scopariae collected in autumn and its preparations.

Artemisia↗

PubMed2002

[Qualitative and quantitative methods of chlorogenic acid in commercial Herba Artemisiae Scopariae].

OBJECTIVE: To develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae. METHOD: TLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (7:2.5:2.5) upper layer as a developing solvent. Chlorogenic acid in 85% methanolic extract was separated on the ODS column with methonal-3% acetic acid solution (15:85) as mobile phase. The detection wavelength was 327 nm. RESULT: The qualitative method is repeatable. Chlorogenic acid in extracts is well separated, relationship of injection amount and peak area is linear (r = 0.9998) within the range of 0.075-0.6 microgram. The average recovery is 100.9% and repeatability is 1.4%. Ten samples purchased from different areas in the country were identified and quantified with the methods. CONCLUSION: The methods and data could be used for quality control.

Artemisia↗

PubMed2002

[Studies on chemical constituents in buds of Artemisia scoparia].

OBJECTIVE: To investigate the chemical constituents in flower buds of Artemisia scoparia. METHOD: The constituents were separated and purified by means of various chromatographic techniques, and their structures were determined on the basis of physical and chemical properties and spectral data. RESULT: Four flavones were isolated and their structures were identified as cirsilineol (I), cirsimaritin(II), arcapillin(III) and cirsiliol(IV) respectively. CONCLUSION: Compounds I, III and IV were isolated from this plant for the first time.

Artemisia↗