Biomedical subjects

Qiang Fu

Publications and source records attributed to Qiang Fu.

At least 73 records · Page 4Linked to original sources

PubMed2005

Bulbocavernosus muscle flap in the repair of complicated vesicovaginal fistula.

AIM: To investigate the transposition of the bulbocavernosus muscle flap for repairing complicated vesicovaginal fistulas. METHODS: Vesicovaginal fistulas were repaired via combined abdominal and perineal approaches. Through an abdominal approach, the fistula and surrounding scar tissue were excised thoroughly. A perineal incision was made between the orifices of the urethra and the vagina, dissecting until the fistula. The vaginal defect was closed through either the abdominal or the perineal approach depending upon its position. Through the abdominal approach, the bladder defect was closed in two layers with the suture lines vertical to each other. The bulbocavernosus muscle was freed through an incision between the labium majus pudendi and the labium minus pudenda, without damaging the pudendal vascular supply. The bulbocavernosus muscle flap was tunneled beneath the labium minus pudendi, and was sutured in place on the bladder wall over the fistula repair site. RESULTS: Nine patients with complicated vesicovaginal fistulas were treated using this technique. After surgery, no symptoms of vagina leakage, urinary incontinence, or urethral stricture were reported by any of the patients, and they reported normal sexual function. CONCLUSIONS: Transposition of the bulbocavernosus muscle flap is an excellent technique with low morbidity and high success rate for repairing complicated vesicovaginal fistulas.

Adult↗

PubMed2005

[The study on strengthening of fluorophlogopite-based dental machinable ceramic].

OBJECTIVE: To study the strengthening of fluorophlogopite-based dental machinable ceramic through developing a new type of calcium-mica-based ceramic. METHODS: Based on the analysis of the crystal structure of the fluorophlogopite ceramic of Dicor MGC, the structure of a new type of calcium-mica-based ceramic was designed and the corresponding composition of the new material was experimented. And the new glass-ceramic was obtained through the treatment of glass preparation and nucleation. Then crystal content of the glass ceramic was analyzed by X-ray diffraction analysis (XRD) and the 3-point bending strength of the new ceramic was recorded. RESULTS: A new type of calcium-mica-based glass ceramic was developed and had the 3-point bending strength of (210.2 +/- 14.7) MPa. Compared with the strength of fluorophlogopite-based dental machinable ceramic, such as Dicor MGC, which was reported in the range of 150 approximately 180 MPa within inclusive studies, the higher strength of the new ceramic was recorded. CONCLUSIONS: The fluorophlogopite-based dental machinable ceramic could be reinforced through internal strengthening.

Aluminum Silicates↗

PubMed2005

[Concentration and determination of strychnine alkaloid in biological fluids].

OBJECTIVE: To establish a new method for determination of strychnine alkaloid in biological fluids based on molecularly imprinted polymers. METHODS: A strychnine molecularly imprinted monolithic column was prepared by in-situ molecularly imprinted technique. The polymer was filled to a 1cm column, and a method was developed to concentrate and determine strychnine alkaloids in biological fluids. RESULTS: the limit of detection of the method was 4.9 ng, and the recoveries were more than 92%. The relative standard deviations were smaller than 6.59%. The linear correlation coefficients of standard curves were 0.999 1 and 0.9966 respectively. This method was applied to concentrate and determine strychnine in plasma and urine of poisoned rabbit. CONCLUSION: The new method could concentrate and simultaneously determine strychnine alkaloids in biological fluids, and it was applied to forensic toxicological analysis.

Alkaloids↗

PubMed2005

[Duplex ultrasonography and internal pudendal arteriogram in the diagnosis of trauma-associated arteriogenic erectile dysfunction (a report of 7 cases)].

OBJECTIVE: To make a comparative study of duplex ultrasonography (DU) and internal pudendal arteriogram (IPA) in the diagnosis of trauma-associated arteriogenic erectile dysfunction (ED). METHODS: Seven patients suffering from arteriogenic erectile dysfunction caused by pelvic fracture trauma underwent comprehensive history inquiries and physical examinations by duplex ultrasonography and internal pudendal arteriogram, which ruled out neurogenic erectile dysfunction. RESULTS: DU suggested penile artery blood flow injury, and IPA indicated artery injury in all the cases, including left internal pudendal artery injury, right or left common penile artery injury and bilateral common penile artery injury. CONCLUSION: Trauma-associated arteriogenic erectile dysfunction may result from common penile artery injury and internal pudendal artery injury. DU is proved valuable for evaluating hemodynamic abnormalities of cavernous artery flow, and IPA useful in locating common penile artery and internal pudendal artery injury. DU can be used as the first line diagnostic means to define trauma-associated arteriogenic ED.

Adult↗

PubMed2005

[Mechanism of urotensin II-stimulated adrenomedullin secretion in human vascular endothelial cells].

OBJECTIVE: To study the mechanism of urotensin II(U II)-stimulated adrenomedullin secretion in human vascular endothelial cells. METHODS: In cultured human vascular endothelial cells (HEVCs), different concentrations of U II was used to stimulate the secretion of Adm, and different inhibitors were used to study the changes in the secretion after block of different signal transduction pathways. The contents of Adm in the medium were detected with radioimmunoassay. RESULTS: U II-stimulated Adm secretion in the HEVCs in a time- and concentration-dependent manner. Adm contents of the treatment groups were comparable with that of the control group (P<0.05 ), and the secretion of Adm could be inhibited by the inhibitor of extracellular signal-regulated protein kinases (PD098059), p38 kinase inhibitor (SB202190), calmodulin inhibitor (W7) and Ca(2+) inhibitor (nicardipine)(P<0.05), but calcineurin inhibitor and protein kinase C inhibitor (H7) had no such effect (P>0.05). CONCLUSION: Ca(2+), MAPK, CaM-PK and p38 signal transduction pathways may play major roles in U II-stimulated secretion of Adm in HVECs.

Adrenomedullin↗

PubMed2005

Phosphorus removal by the multipond system sediments receiving agricultural drainage in a headstream watershed.

Wetland systems in headstream watersheds are important to control the nonpoint source pollutant phosphorus. Experiments were conducted using intact sediment-water columns obtained from the multipond system in Liuchahe watershed of Chaohu Lake to determine its capacity to retain P. It was found that pond sediments had strong P retention ability. For the Hill pond, Village pond and Rice pond, their retention coefficient(A) were 288.3, 279.2 and 260.8 L/m2, respectively. The equilibrium P concentration (EPCw) were 0.016, 0.028 and 0.018 mg/L, respectively. The Hill pond indicated the highest P retention ability. P retained in the pond sediments indicated high stable degree. P removal from the overlying water column into the pond sediments followed a first-order kinetic model. Under the experimental hydrological conditions, the retention time had a positive correlation with the P loading. The multipond system could provide enough retention time to retain P in drainage runoffs. At the P levels evaluated, the sediments of the multipond system are effective sinks to retain P from nonpoint source runoffs.

China↗

PubMed2005

[Effect of dendritic cells co-cultured with cytokine induced killer cells on cytotoxicity against drug resistant K562 cells].

OBJECTIVE: To study the effect of dendritic cells (DC) co-cultured with cytokine induced killer (CIK) cells on cytotoxicity against K562 and K562 drug-resistant (K562/ADM) cells. METHODS: Peripheral blood mononuclear cells (MNC) isolated from healthy adult donors were induced to obtain CIK cells and DC respectively and then these two kinds of cells were co-cultured. The cytotoxicity of the co-cultured cells against K562 and K562/ADM cells was measured with MTT assay. RESULTS: The cytotoxicity CIK cells alone to K562 and K562/ADM cells was (20.0 +/- 1.2)% - (61.1 +/- 2.2)% and (17.5 +/- 2.1)% - (45.2 +/- 3.3)% respectively at low effector to target ratios (2.5 - 20.0). This effect was significantly enhanced by co-culturing with DCs being (25.2 +/- 2.3)% - (70.9 +/- 4.1)% and (22.4 +/- 2.7)% - (62.3 +/- 5.0)%. CONCLUSION: CIK cells showed high cytotoxicity against K562 and K562/ADM cells and the activity could be enhanced by co-culturing with DC.

Cell Proliferation↗

PubMed2005

[Phosphorus sorption capacities of the pond sediments in a headstream agricultural watershed].

The land uses in watersheds have important influence on the sediments of lakes and wetlands. Field and laboratory experiments were conducted to research the phosphorus adsorption by the pond sediments in the pond system in a headstream watershed. Being scattered in different ambient land uses and receiving soil particles from various sources, the pond system provides an excellent research example for the phosphorus sorption characteristics. It was found that the pond sediments had strong phosphorus sorption capacities. The phosphorus adsorption maximum (Smax) ranged from 228 to 974 mg x kg(-1), equilibrium phosphorus concentration (EPC0) ranged from 0.004 to 0.032mg L(-1). The mean degree of phosphorus saturation (DPS) of the pond sediments was only 9.5%. The situation of the ponds can influence the physico-chemical properties of sediments and their sorption characteristics. For Smax, Hill pond >Nonirrigation pond> Rice pond> River pond> Village pond. On the contrary, for EPC0, Hill pond< Nonirrigation pond< Rice pond < River pond < Village pond. The phosphorus adsorption maximum (Smax) was significantly correlated ly correlated with the KCl-extractable phosphorus (KCl-P, r2 = 0.83, p < 0.001). Multiple regression analysis shows that Fe(ox) and KCl-P are the key factors, which can influence phosphorus sorption of the pond sediments.

Adsorption↗

PubMed2005

Features of coding and noncoding sequences based on 3-tuple distributions.

The origin of non-coding sequences, especially introns,is an outstanding issue that has been receiving continuous debate for the last two decades. In the current work we use a mathematical model to characterize DNA sequences and find that the 3-tuple distributions in different reading frames of a given coding sequence differ sharply from each other, while they are almost identical to each other in introns or other non-coding sequences. SREs (Symmetric relative entropies) decrease progressively from coding sequences of primitive prokaryotes to those of advanced eukaryotes and from non-coding sequences of low eukaryotes to those of high eukaryotes with a correlation coefficient of 0.86. In silico evolution experiments show that SREs typical of higher eukaryotic introns can be achieved from prokaryotic coding sequences as the mutation ratio reaches 2/100. The fact that (a total of 25 introns) from all three different genomes S. pombe, C. elegans and H. sapiens searched are found to share high sequence identity with coding regions indicates that at least some introns may have come directly from CDS (coding sequences). We suggest that SREs may be a useful feature for evolutionary study.

Algorithms↗

PubMed2005

[Effect of urotensin II on secretion of adrenomedullin from human vascular endothelial cells].

OBJECTIVE: To study the effect of human urotensin II (HU II) on secretion of adrenomedullin (ADM) from human vascular endothelial cells (HVEC) and its mechanism. METHODS: In cultured HVEC, different concentrations of HUII were used to stimulate the ADM secretion from HVEC, and the inhibitors of different signal transduction pathway were used to investigate their effects on ADM secretion. The contents of ADM in medium were determined by radio immunoassay. RESULTS: HUII stimulated secretion of ADM from HVEC in a time-dependent and concentration-dependent manner. The contents of ADM in the experiment groups were changed compared with that in control group (P < 0.05). The increase of ADM could be inhibited by inhibitor of extracellular signal-regulated protein kinase (PD(98059)), inhibitor of P38 kinase (SB(202190)), inhibitor of calmodulin (W(7)) and inhibitor of Ca(2+) (nicardipine) (P < 0.05). The inhibition ratio in those groups was 68%, 78%, 24% and 25% respectively. But the inhibitor of Calcineurin (CaN) and inhibitor of protein kinase C (H(7)) had no influence on the secretion of ADM from HVEC (P > 0.05). CONCLUSION: The stimulated effect of HUII on the ADM secretion from HVEC may be mediated by Ca(2+), ERKs, CaM-PK and P38 signal transduction pathways.

Adrenomedullin↗

PubMed2005

[Culture and biodegradation performance for phenol-degrading bacterium in high phenol concentration].

The activated sludge was acclimated by the inorganic salt medium containing phenol as the sole carbon source. The phenol-degrading bacterium obtained now can degrade completely phenol at initial concentration of 1 700mg/L at 30 degrees C for 15h, and COD can be degraded by 96.9%. Some factors effecting degradation of phenol and COD were studied, which include initial phenol concentration, quantity of bacterium, temperature and pH. Four strains of bacterium were isolated from the mixed phenol-degrading bacterium. It is shown that the degradation effect of mixed bacterium is superior to that of sole strain.

Bacteria↗

PubMed2004

A novel copper(II) coordination polymer with 2,2'-bipyridyl-3,3'-dicarboxylic acid.

A novel copper(II) coordination polymer, poly[[[aquacopper(II)]-mu3-2,2'-bipyridyl-3,3'-dicarboxylato-kappa4N,N':O:O'] dihydrate], {[Cu(C12H6N2O4)(H2O)].2H2O}n, was obtained by the reaction of CuCl2.2H2O and 2,2'-bipyridyl-3,3'-dicarboxylic acid (H2L) in water. In the molecule, each Cu(II) atom is five-coordinated and lies at the centre of a square-pyramidal basal plane, bridged by three L ligands to form a two-dimensional (4,4)-network. Each L moiety acts as a bridging tetradentate ligand, coordinating to three Cu(II) atoms through its two aromatic N atoms and two O atoms of the two carboxyl groups. The two-dimensional square-grid sheets superimpose in an off-set fashion through the inorganic water layer.

Journal Article↗

PubMed2004

Association between clinical characteristics and expression abundance of RTKN gene in human bladder carcinoma tissues from Chinese patients.

PURPOSE: Bladder carcinoma is the most common urological malignancy in China. Gene mutation may be one of causes of carcinogenesis in the cancer. We therefore investigated the mRNA expression of RTKN gene in clinic malignant bladder carcinoma and explored the relationship between the novel gene and the cancer. METHODS: Total RNA was extracted from 33 surgically resected specimens of bladder carcinoma and 19 specimens of tumor-free bladder tissues. After the optimal reverse-transcription polymerase chain reaction condition was established, the mRNA expression levels of the RTKN gene in the lesions and tumor-free bladder tissues were examined semiquantitatively, and the relationships between expression levels of RTKN and clinical pathological features were analyzed. RESULTS: The expression of RTKN gene mRNA in 33 human bladder carcinoma tissues was significantly higher than that in 19 human tumor-free bladder tissues (0.937+/-0.103 vs. 0.350+/-0.082). The average ratio of RTKN expression in neoplasms to that in tumor-free bladder tissues was 0.350+/-0.164. Based on this ratio the 33 patients were divided into three groups: a down-regulated expression group (n=2), an up-regulated expression group (n=22), and an unchanged group (n=9). Although the chi(2) test demonstrated a statistically nonsignificant differences in RTKN expression between tumor stages Ta, T(1), and T(2) overall in the 33 human bladder carcinoma, the t test showed that there were statistically significant differences between solitary and multiple tumors, between the paired group aged younger or older than 70 years in 27 de novo bladder carcinoma patients, and between the groups with tumor larger or smaller than 2.25 cm(3). CONCLUSIONS: These results suggest that the RTKN gene is involved in bladder carcinogenesis and progression in bladder carcinoma, indicating that RTKN gene could be a molecular target in cancer therapy.

Adenocarcinoma↗

PubMed2004

Cultural tailoring for mammography and fruit and vegetable intake among low-income African-American women in urban public health centers.

BACKGROUND: It is widely accepted that disease prevention efforts should consider cultural factors when addressing the needs of diverse populations, yet there is surprisingly little evidence that doing so enhances effectiveness. The Institute of Medicine has called for randomized studies directly comparing approaches that do and do not consider culture. METHODS: In a randomized trial, 1227 lower-income African-American women from 10 urban public health centers were assigned to either a usual care control group, or to receive a series of six women's health magazines with content tailored to each individual. By random assignment, these magazines were generated from either behavioral construct tailoring (BCT), culturally relevant tailoring (CRT) or both (BCT + CRT). The CRT magazines were based on four cultural constructs: religiosity, collectivism, racial pride, and time orientation. All tailored magazines sent to women ages 40-65 promoted use of mammography; magazines sent to women ages 18-39 promoted fruit and vegetable (FV) intake. Analyses examined changes from baseline to 18-month follow-up in use of mammography and servings of FV consumed daily. RESULTS: Women receiving BCT + CRT magazines were more likely than those in the BCT, CRT, and control groups to report getting a mammogram (76% vs. 65% vs. 64% vs. 55%, respectively), and had greater increases in FV servings consumed daily (+0.96 vs. + 0.43 vs. + 0.25 vs. + 0.59). CONCLUSIONS: Systematically integrating culture into tailored cancer prevention and control interventions may enhance their effectiveness in diverse populations.

Adult↗

PubMed2004

A simple chemical route to selectively eliminate metallic carbon nanotubes in nanotube network devices.

Semiconducting-only single-walled carbon nanotube (SWNT) network field effect transistors (FETs) have been fabricated by selectively reacting all the metallic SWNTs in the devices with diazonium reagents in a controlled manner. We have shown that the concentration of diazonium reagents used is crucial for selectively eliminating metallic SWNTs and keeping semiconducting ones intact. Excessive amounts of diazonium reagents can indiscriminately react with both metallic and semiconducting SWNTs and thus degrade the performance of the devices. This new technique will facilitate the process of fabrication of high-performance SWNT-based electronic devices.

Journal Article↗

PubMed2004

Mobile ambipolar domain in carbon-nanotube infrared emitters.

We spatially resolve the infrared light emission from ambipolar carbon-nanotube field-effect transistors with long-channel lengths. Electrons and holes are injected from opposite contacts into a single nanotube molecule. The ambipolar domain, where electron and hole currents overlap, forms a microscopic light emitter within the carbon nanotube. We can control its location by varying gate and drain voltages. At high electric fields, additional stationary spots appear due to defect-assisted Zener tunneling or impact ionization. The laterally resolved measurement provides valuable insight into the transistor behavior, complementary to electronic device characteristics.

Journal Article↗

PubMed2004

Reversible control of free energy and topography of nanostructured surfaces.

We describe a facile method for the formation of dynamic nanostructured surfaces based on the modification of porous anodic aluminum oxide with poly(N-isopropyl acrylamide) (PNIPAAm) via surface-initiated atom transfer radical polymerization. The dynamic structure of these surfaces was investigated by atomic force microscopy (AFM), which showed dramatic changes in the surface nanostructure above and below the aqueous lower critical solution temperature of PNIPAAm. These changes in surface structure are correlated with changes in the macroscopic wettability of the surfaces, which was probed by water contact angle measurements. Principal component analysis was used to develop a quantitative correlation between AFM image intensity histograms and macroscopic wettability. Such correlations and dynamic nanostructured surfaces may have a variety of uses.

Acrylic Resins↗

PubMed2004

Contribution of stratospheric cooling to satellite-inferred tropospheric temperature trends.

From 1979 to 2001, temperatures observed globally by the mid-tropospheric channel of the satellite-borne Microwave Sounding Unit (MSU channel 2), as well as the inferred temperatures in the lower troposphere, show only small warming trends of less than 0.1 K per decade (refs 1-3). Surface temperatures based on in situ observations however, exhibit a larger warming of approximately 0.17 K per decade (refs 4, 5), and global climate models forced by combined anthropogenic and natural factors project an increase in tropospheric temperatures that is somewhat larger than the surface temperature increase. Here we show that trends in MSU channel 2 temperatures are weak because the instrument partly records stratospheric temperatures whose large cooling trend offsets the contributions of tropospheric warming. We quantify the stratospheric contribution to MSU channel 2 temperatures using MSU channel 4, which records only stratospheric temperatures. The resulting trend of reconstructed tropospheric temperatures from satellite data is physically consistent with the observed surface temperature trend. For the tropics, the tropospheric warming is approximately 1.6 times the surface warming, as expected for a moist adiabatic lapse rate.

Journal Article↗