Biomedical subjects

Qiu-Ling Liu

Publications and source records attributed to Qiu-Ling Liu.

5 recordsLinked to original sources

PubMed2006

Fluorescent multiplex amplification of three X-STR loci.

This study was carried out to evaluate the value of three X-STR loci (DXS6803, DXS981and DXS6809) in forensic application and thereby investigate their polymorphism. The primer for each locus was labeled with fluorochrome 6-FAM. A fluorescent multiplex PCR for simultaneously amplifying three X-STR loci was set up. The PCR products that were obtained were analyzed using capillary electrophoresis and ABI PRISM 3100 Genetic Analyzer, with GENESCAN Analysis Software. When 340 male and 195 female individuals of Han population in China were tested, 13, 12, and 11 alleles were observed for DXS6803, DXS981 and DXS6809, respectively. One hundred and eighty three haplotypes were detected in the male individuals. The haplotype diversity reached 0.9926. The results show that the three loci of the multiplex system provide significant information on polymorphism for forensic identification and paternity testing, particularly for complicated paternity deficient cases.

Chromosomes, Human, X↗

PubMed2006

[Haplotypes of DXS10011 and DXS8377 in Guangdong Han individuals].

OBJECTIVE: To investigate genetic polymorphism of two X chromosome specific STR: DXS10011 and DXS8377 in male samples from Guangdong Han population. METHODS: The DNA samples were amplified by PCR and analyzed by polyacrylamide gel electrophoresis followed by silver staining. RESULTS: Among 113 samples, 20 alleles were found for DXS1011 and 12 alleles for DXS8377. Also, 72 DXS10011-DXS8377 haplotypes were shown. The most common haplotypes only occurred three times. When only female children were tested in motherless case, the exclusion probability of paternity was 0.9588 for DXS10011-DXS8377 haplotypes. Investigations in 83 family trios with female children and 29 pedigrees with two children suggested a co-dominant X-linked inheritance; mutations were not found. CONCLUSION: Our data indicate that DXS10011 and DXS8377 are highly informative X chromosome markers for complicated kinship analysis.

Alleles↗

PubMed2004

[Short tandem repeat polymerase chain reaction used in prenatal diagnoses of the zygosity and common chromosomal trisomies in multiple pregnancies].

OBJECTIVE: To evaluate the value of short tandem repeat polymerase chain reaction (STR-PCR) in identification of zygosity in multiple pregnancy and detection of the common chromosomal trisomies. METHODS: Amniotic fluid or fetal blood samples were collected from 38 fetuses in 17 multiple pregnant women who had indications for prenatal diagnosis or planed to be performed feticide. Of the 17 cases there were 13 sets of twins (26 fetuses) and 4 sets of triplets (12 fetuses). Parental blood samples were collected. STR-PCR technique was employed to determine the zygosity and detect trisomy 21, trisomy 18, trisomy 13, and trisomy sex chromosomes. Amniotic fluid or blood samples were collected from 18 singleton pregnant fetuses as controls for detecting chromosomal trisomies. RESULTS: All 7 cases with gestation by assisted reproductive techniques were multizygotic. In 7 cases associated with one fetal malformation, only one set of twin was dizygotic. There were no above mentioned trisomies found in the multiple pregnancies while 2 trisomy 21, one trisomy 18 and 13 respectively were detected in the singleton pregnant group. Feticide was performed on 4 multizygotic cases in which 3 were triplets and 1 was twin associated with an abnormal fetus. Following-up showed that there was no any bad effect after procedure. CONCLUSION: STR-PCR can be used in identifying the zygosity correctly and quickly while being used in detecting chromosomal trisomies. It facilitates monitoring multiple pregnancy as well as selecting appropriate methods of feticide.

Adult↗

PubMed2003

[Four Y-STR multiplex system by silver staining].

A multiplex PCR system has been developed to amplify 4 Y-chromosome specific short tandem repeats (STR), DYS391, GATA-A4, GATA-A10 and GATA-H4, simultaneously. PCR products were separated by polyacrylamide gels electrophoresis followed by silver stain. When 311 unrelated males from the Han population in Guangdong were detected by the multiplex system, DYS391, GATA-A4, GATA-A10 and GATA-H4 showed 5, 7, 6 and 5 alleles respectively. Total 98 haplotypes could be identified. Gene diversity value for the 4 STR was 0.4623, 0.6972, 0.7173 and 0.6015 respectively. The gene diversity value for the haplotypes of the 4 Y-STR reached 0.9755. The four Y-STR multiplex system will be very powerful for establishing Y-STR database, exploring human origin, paternity testing and personal identification.

English Abstract↗

PubMed2003

[Analysis of subpopulation in Guangdong Han population].

To investigate the subpopulation structure within the Han Population in Guandong area, a total of 471 DNA samples from five populations in Guandong Province, including Guangzhou, Foshan, Dongguan, Jiangmen and Zhongshan-Zhuhai region, were genotyped at 15 STR(short tandem repeats) markers. Hardy-Weinberg tests were performed, allele frequencies were compared, and the genetic coancestry coefficient(FST) was estimated. The results did not show significant departure from Hardy-Weinberg equilibrium in the total population. Difference of allele frequencies among these populations was not observed, and the coancestry coefficient(FST)was less than 0.01. Subpopulation structure within Han Population in Guandong Province could not be detected.

English Abstract↗