PubMed Health⌕ Search

Biomedical subjects

Qiu-liang Wu

Publications and source records attributed to Qiu-liang Wu.

18 recordsLinked to original sources
PubMed2006

[Treatment outcome and prognosis of 112 patients with nasal and nasopharyngeal peripheral T cell lymphomas].

OBJECTIVE: To retrospectively analyze the treatment outcomes and prognostic factors of nasal and nasopharyngeal peripheral T cell lymphomas (PTCL) patients. METHODS: One hundred and twelve patients with pathologically confirmed nasal and nasopharyngeal PTCL were included, among which 39 were CD56(+) NK/T cell lymphomas. The median pre-treatment disease course was 4 months. 84 were males and 28 females median age was 46 years. The tumors mainly involved nasal cavity (88 cases) and/or nasopharynx (50 cases) and adjacent structures, and 83 cases with extra-cavity diseases. 91.1% of the patients had Ann Arbor I(E)/II(E) diseases. The International Prognostic Indices (IPI) were less than 2 scores in 78.8% of the patients. Seventy two patients received combined chemo-radiotherapy, 32 chemotherapy only, 3 radiotherapy only and 5 no any treatment. RESULTS: Median follow-up duration was 42 months. Chemotherapy achieved a complete remission (CR) rate of 34.4% for initial treatment, and of 65.1% after primary treatment. The local tumor controlled rate was 50.5%, and the median time to tumor progression (TTP) was 11 months. There were evidences of systemic relapse in more than 30% of the patients. The extra-cavity tumors usually had a shorter TTP (r(s) = -0.191, P = 0.024). The progress-free survival and overall survival rates were 38.8% and 52.4% at 3 years, and 34.9% and 44.8% at 5 years respectively. Univariate analysis showed that favorable prognostic factors for survival were pre-treatment course > 3 months, earlier clinical stage, non NK/T lymphoma, no skin involvement, lower IPI, CR after initial chemotherapy, radiotherapy, CR after primary treatment and local tumor controlled. Multivariate analysis showed that, pre-treatment course > 3 months (P = 0.011), non NK/T lymphoma (P = 0.007), CR after initial chemotherapy (P = 0.008) and radiotherapy (P = 0.000) were favorable prognostic factors for survival. CONCLUSIONS: Although most nasal and nasopharyngeal peripheral T-cell lymphomas were diagnosed at early stage diseases, some of them were highly aggressive with poor prognosis, particularly CD56(+) NK/T cell lymphomas. Combination chemo/radiotherapy, though remained principal treatments, more effective therapeutic modalities are expected.

Adolescent↗
PubMed2006

[High expression of nucleophosmin/B23 in hepatocellular carcinoma].

OBJECTIVE: To study the expression of nucleophosmin/B23 (B23) in tumor cells of hepatocellular carcinoma (HCC) and its clinicopathologic significance. METHODS: Mouse monoclonal antibodies against B23 were raised by recombinant protein and hybridoma technology. Immunohistochemical study for B23 was performed on 103 cases of HCC, 12 cases of focal nodular hyperplasia and 17 cases of native liver tissue adjacent to hepatic hemangioma. Fresh specimens from 10 cases of HCC and the adjacent liver tissue were also collected for reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis. The expression of B23 was analyzed and compared with that of proliferative cell nuclear antigen (PCNA) in these specimens. RESULTS: RT-PCR and Western blot analysis showed that B23 expression in HCC was higher than that in adjacent liver tissue. Statistically significant difference in expressions of B23 and PCNA were also noted in the four groups studied (P < 0.01). B23 and PCNA expressions in HCC were higher than those in the other three groups (P < 0.01). There was also a statistically significant correlation between B23 and PCNA expressions amongst the four groups (r = 0.4769, P < 0.01). Besides, B23 expression in HCC correlated with pathologic tumor grading, serum alpha-fetal protein levels and cirrhotic status (P < 0.05). CONCLUSIONS: B23 expression in HCC was significantly higher than that in liver tissues with non-malignant diseases. B23 may be used as a marker for neoplastic changes in liver cells and thus has potential clinicopathologic application.

Biomarkers, Tumor↗
PubMed2005

[Tissue engineered bone reconstruction with modified PLGA/Type-I collagen compound scaffold].

OBJECTIVE: To fabricate bone grafts by bone marrow stromal cell combined with modified PLGA/Type-I collagen compound scaffold using tissue engineering method. METHODS: The modified PLGA/Type-I collagen compound scaffold was fabricated. The rabbit primary cultured osteoblasts were identified and seeded onto the modified compound scaffold for one week in vitro. The adhesion and growth of cells were observed with scanning electron microscope. The complex of cells and scaffold was implanted into the subcutaneous region of rabbits and new bone formation was evaluated. RESULTS: The rabbit bone marrow stromal cells were induced and differentiated into osteoblasts. The adhesion and growth of osteoblasts in cluster were observed on the surface of scaffolds. New bone formation was observed at one month postoperatively and active osteoblasts were found on the surface of the newly formed bone in vivo. CONCLUSION: The complex of PLGA and type-I collagen is an appropriate biodegradable scaffold and can be applied in bone tissue engineering.

Absorbable Implants↗
PubMed2005

[Clinical and pathological reassessment of 493 cases of non-Hodgkin's lymphomas according to current WHO classification of lymphoid neoplasms].

OBJECTIVE: To investigate the clinical and pathological features of non-Hodgkin's lymphoma (NHL) and to evaluate the applicability of the new WHO classification of lymphoid neoplasms. METHODS: According to the new WHO classification, a total of 500 cases of non-Hodgkin's lymphoma diagnosed during the period 1992 - 2003 were reviewed and reappraised with their morphological, immunological and clinical characteristics. Clinical survival analysis was performed in 156 cases that accompanied with follow-up data. RESULTS: Among 500 cases previously diagnosed as lymphomas, 493 cases (98.6%) were confirmed to be NHL, of which B-cell neoplasms was 69.0% and T/NK-cell neoplasms 29.8%. Overall, 6 subtypes including diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), unspecified peripheral T-cell lymphoma (PT-un), precursor T-lymphoblastic lymphoma (T-LBL), extranodal marginal zone B-cell lymphoma of MALT type (MALT) and B-small lymphocytic lymphoma (B-SLL) were among the most common subtypes. In pediatric and young patient populations, the most common subtypes were LBL, DLBCL and Burkitt's lymphoma. The frequency of LBL in all patients, especially in the juniors, was much higher than those reported outside Mainland China, and the frequency of FL was much higher than the reported in Mainland China. The frequency of FL was much higher than the reported in Mainland China. Clinical survivals among different histological subtypes of NHL varied considerably with statistic significance (P < 0.001). Marginal zone B-cell lymphoma and SLL demonstrated the best prognosis, LBL and PT-un both the worst, whereas DLBCL and FL had an intermediate prognosis, however, subgrouping of FL according to WHO classification did not reveal a significant survival difference (P > 0.05). CONCLUSIONS: Basing upon the results of a comprehensive survey on the morphologic features, immunophenotyping and clinical data of the above cases, the new WHO classification of lymphoid neoplasms is practical and easily applicable for routine pathological evaluation of lymphoproliferaive disorders and in guiding the clinical management. It appears that the diagnostic and grading criteria for FL in Mainland China need to be re-evaluated.

Adolescent↗
PubMed2005

[Effects of arsenic trioxideon the cell apoptosis and hTERT mRNA of human tongue cancer cells].

OBJECTIVE: To study the effects and its mechanisms of arsenic trioxide (As2O3) on cell growth and human telomerase reverse transcriptase (hTERT) of human tongue cancer cells (Tca8113 cell line). METHODS: The growth inhibition rates of Tca8113 by various concentrations of As2O3 were detected by MTF method. Cell apoptosis was detected by FCM labeled with Annexin V-FITC. hTERT gene expression was detected by RT-PCR method. hTERT protein of Tca8113 cells was determined by Western blot assay. RESULTS: The results showed that As2O3 could inhibit the growth of Tca8113 effectively and apoptotic rate of Tca8113 cells was obviously increased in a dose and time-dependent manner. (83.40 +/- 7.31)% cells treated with 5 micromol/L As2O3 were inhibited on 72 hour point, the early apoptosis rate reached on 26.40% +/- 3.42% at that time. Moreover hTERT mRNA and protein were decreased depended on the dose and time of As2OC3, mRNA expressions of hTERT in test groups were greatly lower than that of control group on 72 hour point. CONCLUSION: It was suggested that As2O3 could significantly inhibit the growth of Tca8113 cells by inducing causing cell apoptosis and down-regulating the expression of hTERT mRNA gene and protein which might be one of its action mechanisms.

Apoptosis↗
PubMed2005

[Effects of tea polyphenols on cell proliferation and hTERT of human Tca8113 cell lines].

OBJECTIVE: To investigate the effects of tea polyphenols (TP) on cell proliferation and human telomerase reverse transcriptase (hTERT). METHODS: The experiment was divided into tea polyphenols 0.100 g/L, tea polyphenols 0.050 g/L and blank control groups. The inhibitory ratio of cell proliferation was assayed with MTT, and hTERT mRNA was detected by RT-PCR, and hTERT protein analyzed by Western-blotting. The data were analyzed by one-way ANOVA and Student-Newman-Keuls of SPSS 11.0 for windows. RESULTS: Cell proliferations were significantly inhibited after exposure to TP. The proliferation inhibiting rate of TP 0.025, 0.050, 0.100, 0.200 g/L on Tca8113 cell at 72 h was 69.75% +/- 3.24%, 63.17% +/- 3.19%, 50.35% +/- 4.21% and 34.75% +/- 3.71%, respectively (F = 270.19, P < 0.05). At the end of 72 h, the hTERT mRNA expression in TP 0.100 g/L, 0.050 g/L and control group were 0.32 +/- 0.05, 0.41 +/- 0.04, 0.72 +/- 0.05, respectively (P < 0.05). The Western-blotting assay showed that hTERT protein was also decreased by tea polyphenols compared to control group. CONCLUSIONS: Tea polyphenols could inhibit the proliferation of Tca8113 cells and expression of the hTERT mRNA and protein in Tca8113 cell lines. This effect might be one of the mechanisms for anticancer function of tea polyphenols.

Carcinoma, Squamous Cell↗
PubMed2005

[Clinicopathological features and prognosis of postirradiation osteosarcoma in nasopharyngeal carcinoma].

OBJECTIVE: Post irradiation osteosarcoma (PIOS) in patients with nasopharyngeal carcinoma (NPC) is rare and a potential late complication of radiation. We investigate its clinicopathological features and prognosis. METHODS: 426 cases of bone sarcomas in Cancer Center of Sun Yat-sen University, China between 1964 and 2003 were reviewed retrospectively. Fifteen patients were determined to have PIOS after radiation of NPC. Its prevalence rate, onset time, site, image features, and treatment were described. Kaplan-Meier analysis was used to determine the relative prognostic factors. RESULTS: In 12 patients undertaken radical surgery, one patient had residual tumor and six patients presented tumor recurrence five to 19 months (mean of nine months) after surgery. All patients survived seven to 41 months with a mean of 18 months. The one-year and two-year survival rates were 60% and 24% respectively. Female patients with large area of tumor bone formation in images had better survival than male patients without or few tumor bone formation. Age, radiation dosage, onset time of PIOS, tumor size, and treatment were probably not significant factors to prognosis. CONCLUSIONS: PIOS in patients with NPC is a high malignant disease and often has poor prognosis. Surgery with pre-and post-operative chemotherapy might be a way to improve its survival.

Adolescent↗
PubMed2005

[T-cell/histiocyte-rich B-cell lymphoma: histology, immunophenotype and differential diagnosis].

OBJECTIVE: To study the histology, immunophenotype and differential diagnosis of T-cell/histiocyte-rich B-cell lymphoma (TCRBCL). METHODS: A review of 245 cases of so-called Hodgkin lymphoma diagnosed during the period from 1980 to 2000 in 3 hospitals in Guangzhou, 8 cases were reclassified as TCRBCL, according to the 2001 World Health Organization classification of lymphoid neoplasms. An additional 8 cases of TCRBCL were retrieved from consultation files, as well as routine biopsy cases encountered between 2000 and 2004. Immunohistochemical studies were performed on paraffin-embedded tissue by SP technique in order to study the immunophenotype of the large neoplastic cells (CD20, CD79a, CD3, CD45RO, CD15, CD30, CD10, bcl-6 and EMA) and background non-neoplastic cells (CD3, CD8, CD20, CD45RO, CD79a, CD57, CD68, CD21, CD35, cyclin D1, TIA-1). In-situ hybridization for EBER 1/2 and immunoglobulin heavy chain gene rearrangement study were also performed in 4 and 4 cases respectively. RESULTS: Among the TCRBCL cases studied, there were 8 males and 8 females. The age of patients ranged from 10 to 68 years old (mean = 40.3 years old). All had lymphadenopathy and hepatosplenomegaly. On presentation, 3 cases belonged to stage II, 10 cases stage III and 3 cases stage IV. Histologically, scattered atypical large neoplastic cells were seen in a background of small lymphocytes and sometimes histiocytes. The large cells exhibited CD20+, CD79a+, EMA+, CD15- and CD30- phenotype. On the other hand, the background small lymphocytes were CD3 and CD45RO-positive. Most of these background T cells expressed CD8 and TIA-1, while they were mostly CD57-negative. The histiocytic cells were CD68-positive; and CD21 and CD35-positive follicular dendritic cell meshworks were absent. In-situ hybridization for EBER 1/2 showed negative nuclear signals. Immunoglobulin heavy chain gene rearrangement study revealed clonal pattern in all the 4 cases tested. CONCLUSIONS: TCRBCL is a rare subtype of lymphoma, with distinctive histology and immunophenotype. The above features are helpful in delineating this entity from Hodgkin lymphoma, reactive lymphoid hyperplasia and lymphomatoid granulomatosis.

Adolescent↗
PubMed2004

[Quantitative analysis of Epstein-Barr virus DNA in plasma and peripheral blood cells in patients with nasopharyngeal carcinoma].

OBJECTIVE: To quantitative analysis of Epstein-Barr virus (EBV) DNA levels in plasma, peripheral blood cells (PBCs) and tumor tissue in nasopharyngeal carcinoma (NPC), to investigate the relationship between EBV-DNA levels and clinical parameters. METHODS: Blood of 150 primary NPC and 49 corresponding tumor tissues, 47 nasopharyngitis tissues and blood of 75 controls were entered this investigation. Plasma and PBCs were isolated for quantitative detection of EBV-DNA by using real-time quantitative PCR (RQ-PCR). The paraffin-embedded tissue sections were conducted to quantitative detection of EBV-DNA, and EBER1 in situ hybridization (ISH) for calculating the percentage of positive cells on the tissue section. RESULTS: Plasma EBV-DNA levels and detecting rate in NPC before treatment (median 82 500 copies/ml, 92%) were significantly higher than that in NPC after treatment (median 0 copy/ml, 19%) and in controls (median 0 copy/ml, 12%) (P < 0.05), whereas there was no significant difference between NPC after treatment and controls (P > 0.05). There was no significant difference of PBCs EBV-DNA load and detecting rate in NPC before (0 copy/actin, 24%) and after treatment (0 copy/actin, 14%), as well as in controls (0 copy/actin, 16%) (P > 0.05). Plasma EBV-DNA level was not correlated to PBCs EBV-DNA load in NPC before (P = 0.92) and after treatment (P = 0.27), and controls (P = 0.74). EBV-DNA level (27.8 copies/actin) in NPC tumor tissues was significantly higher than that in nasopharyngitis (0 copy/actin) (P < 0.05), and was positively correlated to the ratio of EBER1 positive cells to total cells on the NPC section. In NPC patients, plasma EBV-DNA level was significantly increased in TNM stage I (2500 copies/ml), II (32 590 copies/ml), III (86 000 copies/ml) and IV (166 200 copies/ml), whereas there was no significantly difference of PBCs EBV-DNA loads in difference stages of NPC. CONCLUSION: Plasma EBV-DNA level is a more sensitive and reliable biomarker than PBCs EBV-DNA loads for reflection the tumor volumes in NPC patients. Plasma EBV-DNA detection will improve TNM staging in NPC clinical practice on molecular level.

Biomarkers, Tumor↗
PubMed2004

[The relationship between lymphangiogenesis and cervical lymph node micrometastasis in oral squamous cell carcinoma].

OBJECTIVE: To probe the relationship between lymphangiogenesis and cervical lymph node micrometastasis in oral squamous cell carcinoma. METHODS: The microlymphatic vessel density was detected with enzyme histo-chemical method in 47 cases of oral squamous cell carcinoma and 10 cases of normal oral mucosa; 355 lymphnodes were detected with immunohistochemically using monoantibody AE3. RESULTS: The mean MLVD was 14.04 +/- 6.92 in tumor group, or 5.48 +/- 2.62 in normal group. The difference was (P < 0.001). The percentage of tumor with expression CK was 48.9%. The mean MLVD was 16.94 +/- 5.43 in CK positive group, or 11.26 +/- 5.00 in CK negative group, There was difference significant (P < 0.001). CONCLUSIONS: Lymphangiogenesis plays a key role in cervical lymph node micrometastasis of oral squamous cell carcinoma.

Carcinoma, Squamous Cell↗
PubMed2004

[Expression related to vascular endothelial growth factor C and induced nitride oxide synthesizase in lymph node micrometastasis of oral squamous cell carcinoma].

OBJECTIVE: To investigate the relationship of vascular endothelial growth factor C (VEGF-C) and induced nitride oxide synthesizase (iNOS) expression in lymph node micrometastasis of oral squamous cell carcinoma. METHODS: Samples were obtained from 47 cases of oral squamous cell carcinoma and 15 cases with normal oral mucosa, VEGF-C and iNOS mRNA expression were detected by RT-PCR method. Lymph node micrometastasis of 10 normal lymph nodes and 355 lymph nodes from 47 cases of oral squamous cell carcinoma was detected with immunohistochemical reaction in cytokeratin antibody. RESULTS: The percentages in tumors with higher expression were 57.4% for VEGF-C, 68.1% for iNOS (P < 0.05). They were significantly higher than that of normal groups. Significant positive relationship was found between VEGF-C and iNOS (P < 0.01). The positive rate of cytokeratin (CK) was 48.9%. Significant positive relationship was found between VEGF-C and CK, iNOS and CK (P < 0.01). The expression rates of CK in positive group of VEGF-C and iNOS were 63.0%, 65.6% respectively, and were significant higher than negative groups. CONCLUSION: Expression of VEGF-C and iNOS in lymph node micrometastasis of oral squamous cell carcinoma is significant related.

Carcinoma, Squamous Cell↗
PubMed2003

[Giant pathologic section in the study of optimal length of surgical resection for esophageal carcinoma].

OBJECTIVE: To study the optimal surgical resection length for esophageal carcinoma. METHODS: Specimens of seventy patients with esophageal squamous cell carcinoma resected and collected in our hospital were made into pathologic giant sections. Direct intramural infiltration, multicentric carcinogenic lesion and leaping metastasis were observed in the large slice by microscope. The actual length during the operation was calculated by the ratio of shrinkage. RESULTS: Direct intramural infiltration was found in 51 (72.9%) patients, 39 proximal and 36 distal to the tumor. The mean length of direct intramural infiltration was 0.9 +/- 0.8 cm (4.0 cm maximum) proximally and 0.5 +/- 0.3 cm (2.0 cm maximum) distally. Multicentric carcinogenic lesion was found in 11 (15.7%) patients, 5 proximally, 8 distally and 2 on both sides. Proximal to the tumor, the mean distance between the multicentric carcinogenic lesion and the main lesion plus the length of the multiple carcinogenic lesion was 3.2 +/- 1.5 cm (4.7 cm maximum). Distal to the tumor, it was 3.6 +/- 2.4 cm (9.1 cm maximum). Leaping metastasis was found in 9 (12.9%) patients, 7 proximally and 4 distally. The mean distance between the leaping metastasis and the main lesion plus the length of the leaping metastatic lesion was 1.9 +/- 0.6 cm (2.9 cm maximum) proximally and 1.4 +/- 1.0 cm (2.7 cm in maximum) distally. CONCLUSION: The optimal surgical resection length for esophageal carcinoma should be at least 5 cm proximal to the tumor and total length on the distal side.

Esophageal Neoplasms↗
PubMed2002

[Expression and clinical significance of p53 and c-erbB2 in geriatric women with cervical carcinoma].

BACKGROUND & OBJECTIVE: Few reports have reported on molecular biological study of cervivcal carcinoma in eldely woman, So the authors designed this study to evaluate the expression and clinical significance of tumor suppressor gene p53 and oncogene c-erbB2 in the progression of this kind of cancer. METHODS: Seventy-nine patients with cervical cancer and 23 with cases of normal cervical epithelium in geriatric people were investigated immunohistochemically for expression of p53 and c-erbB2 in paraffin sections. RESULTS: The expression of p53 in ICC (invasive cervical carcinoma) and normal epithelium were 18.98% and 0%, respectively (P < 0.01), and the rate of c-erbB2 oncoprotein were 43.04% and 34.78%, respectively (P = 0.63). p53 staining in ICC with grade 1, 2, and 3 were 50.00%, 11.76%, and 17.14%, respectively (P = 0.01), and frequency of c-erbB2 oncoprotein were 80.00%, 38.24%, and 37.14%, respectively (P < 0.01). c-erbB2 staining in patients with stage I, II, and III or more were 33.33%, 38.46%, and 51.61%, respectively (P < 0.01), while p53 overexpression was not associated with clinical stage. Percentage of p53 staining in the groups with negative lymph nodes and positive ones were 6.67% and 41.67%, respectively (P = 0.01). While c-erbB2 expression was not associated with lymph node status. CONCLUSION: The p53 overexpression was an important factor in the process of carcinogenesis of elder women with cervical cancer and a predictive indicator for lymph node status. c-erbB2 gene may be activated in the early stage of pathogenesis of cervical carcinoma in geriatric patients.

Aged↗
PubMed2002

[Study of CD44v6 protein expression in intraductal papilloma and its malignant transformation of breast].

BACKGROUND & OBJECTIVE: CD44v6 plays an important role in the malignant transformation of breast epithelia and is differentially expressed in normal and neoplastic breast tissue. The diagnostic value of this marker in differentiating malignant from benign breast lesions has not been well examined. This study was designed to evaluate the diagnostic value of CD44v6 protein in distinguishing between benign and malignant papillary lesions of the breast, which is be difficult morphologically, by determining the level of CD44v6 expression in different lesions of the breast. METHODS: The level of CD44v6 protein was detected by immunohistochemistry in 111 paraffin-embeded blocks. RESULT: The positive expressive rates of CD44v6 protein in the basal epithelial cells of the normal tissue, intraductal papilloma, and its malignant transformation, intraductal carcinoma, invasive ductal carcinoma were 95.00%, 85.72%, 66.66%, 66.66%, and 0.00% respectively. The difference between the intraductal papilloma and its malignant transformation was not significant(P > 0.05). However, the positive expressive rates of CD44v6 protein in the luminal epithelial cells of the above lesions were 5.00%, 20.41%, 83.34%, 93.33%, and 100% respectively. There was difference between intraductal papilloma and its malignant transformation(P < 0.01). CONCLUSION: CD44v6 detection by immunohistochemistry is useful in distinguishing intraductal papilloma from its malignant transformation of the breast.

Breast Neoplasms↗
PubMed2002

[Advancement of studying the biological characteristics of nasopharyngeal carcinogenesis].

This review is to summarize the main achievements of studying the biological characteristics of nasopharyngeal carcinogenesis performed by the authors' research team and the recent advancement in this field during the past 5 years as well as to explain the authors' viewpoints concerning the nasopharyngeal carcinogenesis. In order to study the nasopharyngeal carcinogenesis, more than 20,000 nasopharyngeal carcinoma biopsies and more than 600 nasopharyngeal biopsies of Epstein-Barr virus seropositive persons who had been got follow-up over 12 years, were collected. In addition, nude mice and cell lines were also to be utilized. Besides histopathological staining, methods of molecular biology, including in-situ hybridization, PCR etc. were applied. Up to date, 26 papers concerning this subject had been formally published in the medico-biological journals at home and abroad. The results and conclusions were as follows. (1) The squamous metaplasia, epithelial dysplasia, carcinoma in-situ and microinvasive carcinoma are the morphogenetic sequence found in nasopharyngeal carcinogenesis. (2) This morphogenetic sequence is frequently observed in a restricted area of nasopharyngeal mucosal epithelium, representing as an appearance of field carcinogenesis. (3) EB virus may play a critical role in nasopharyngeal carcinogenesis, since EB virus DNA and small RNAs could be detected in epithelial dysplasia first and several viral encoded products, especially LMP1, might be expressed in dysplasia, carcinoma in-situ and microinvasive carcinoma. (4) The multigenic mechanisms, including interactions between EB viral genes encoded products and the products abnormally expressed step by step from genes related to cell-cycle regulation, are the molecular events involved in nasopharyngeal carcinogenesis. (5) The cellular immunity of individuals should also be considered as an important factor influencing nasopharyngeal carcinogenesis, because EB virus specific cytotoxic T-lymphocytes could not only be observed in carcinoma nests but also detected in peripheral blood.

Animals↗
PubMed2002

[Clinical analysis of 57 patients with ovarian dysgerminoma].

BACKGROUND & OBJECTIVE: Ovarian dysgerminoma is an uncommen ovarian malignancy. Its clinical features are special and there are many factors influencing the prognosis. If treated properly, the patient can be cured. Otherwise it may endanger the patient's life. The aim of this study is to investigate the clinical features and factors related to prognosis of ovarian dysgerminoma. METHOD: The data of 57 patients with pure Ovarian Dysgerminoma were analyzed retrospectively, who were admitted to Cancer Center, Sun Yat-sen University of Medical Sciences from January 1, 1964 to December 31, 2000. RESULTS: The main clinical features were abdominal mass (56.1%), abdominal pain (21.1%), abdominal swelling(17.5%), vaginal bleeding(5.3%), genital tract abnormality (5.3%). Twenty-six patients had stage Idiseases, 8 stage II, 9 stage III, 1 stage IV and 13 recurrent and persistent diseases. The uterus was involved in 41.2% patients with stage II-III diseases. Combined modality was given to 52 cases and single-method treatment 5 cases. The overall 5-year and 10-year survival rate for stage I-IV was 80.1% and 70.0% respectively. The 5-year survival rate for stage I was 100%, stage II 55.2%, stage III 55.6%, stage IV 0% and recurrent and persistent diseases 72.7%. In the group of stage I, 12 patients received adnexectomy and 14 patients underwent hysterectomy and adnex removal, there was no significant difference between the 5-year and 10-year survival rate (all 100%) (P < 0.05). Of 23 patients in stage I group to whom only chemotherapy were given after operation, 19 cases received 3 or more courses and were well being without recurrence; four patients received only one course and one of them recurred 21 months after operation. In the group of stage II and III cases the 5-year survival rate was 86.7% for those whose chemotherapy courses were > or = 4 and 25.0% for patients who received less than 4 courses of chemotherapy (P < 0.05). CONCLUSIONS: The prognosis of ovarian dysgerminoma is closely related to disease stage and modality of treatment. Fertility-preserving operation can be considered in early-staged patients, and be careful of doing so in middle-late staged cases. Good results can be achieved with operation-based combined modality in recurrent patients.

Adolescent↗
PubMed2002

[Expression and significance of p53 and proliferating cell nuclear antigen in malignant tumor of maxillary sinus].

BACKGROUND & OBJECTIVE: p53 and proliferating cell nuclear antigen (PCNA) play an important role in the development of malignant tumor. This study was designed to evaluate the role of p53 and PCNA in the development of malignant tumor of maxillary sinus by detecting their expression in malignant, benign, and inflammatory lesions of maxillary sinus. METHODS: The expressions of p53 and PCNA were detected by immunohistochemistry in 108 malignant, 19 benign, and 8 inflammatory tissues of maxillary sinus. Rank sum test was used and the levels of positive degree of p53 and PCNA were represented by mean rank. RESULTS: The mean ranks of p53 and PCNA were 42.8 and 47.9 in inflammatory group and 50.3 and 46.8 in benign group, but 73.0 and 73.2 in malignant group, respectively. In malignant group, mean rank of p53 in squamous cell carcinoma (SCC) was 60.7, which was higher than that in adenocarcinoma (43.9). The differences were significant (P < 0.05). While from stage I to stage IV, the mean ranks of p53 were 46.6, 50.1, 56.1, and 55.0 and the mean ranks of PCNA were 60.5, 48.8, 56.1, and.

Adenocarcinoma↗