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Biomedical subjects

R A Bonar

Publications and source records attributed to R A Bonar.

7 recordsLinked to original sources
PubMed1977

Canine urinary bladder epithelial cells: preparation for cell culture by enzyme dispersion.

In a qualitative and quantitative study of enzymic dispersion of cells from the mucosal layer stripped from canine urinary bladder, trypsin was found to be equal or superior to the other enzymes tested for dispersal of urothelial cells specifically. Collagenase or collagenase plus trypsin served to disperse the whole tissue. A procedure for recovering the urothelial cells as a single-cell suspension and establishing them in culture is presented. The morphology, culture behaviour, and chromosome complement of these cells is described.

Animals↗
PubMed1977

Development and application of basic research techniques in bladder cancer research.

The growth of transitional epithelial cells with different growth media and growth supports was examined. Sephadex G-10, Bio-Gel P-20, Bio-Glas-1000, DEAE-Sephadex A-50, DEAE-cellulose, CM-Sephadex C-50, acid-soluble collagen, and immobilized collagen fibers were used to enhance plating efficiency. Acid-soluble collagen layers optimally increased the plating efficiency of primary cultures of bladder carcinoma. Media alterations with serial combinations of fetal calf, newborn calf, calf, bovine, and bull serum with minimum essential medium, Roswell Park Memorial Institute Tissue Culture Medium 1640, Connaught Medical Research Laboratories Medium 1066, Medium 199, Grand Island Biological, National Cancer Tissue Culture 135, 1415, McCoy's 5A, and National Cancer Institute medium were established. No promotion of cell division was noted with any one of these basic medium formulations.

Animals↗
PubMed1976

Acute tubular necrosis. An experimental model detailing the biochemical events accompanying renal injury and recovery.

Male Charles River mice, divided into control or experimental groups, received on Day 0 either sterile 0.3 MNaHCO3 in 0.9 per cent saline (pH7.4) intraperitoneal injection or pteroylglutamic acid (200 mug per body weight), similarly buffered to pH7.6, and were sacrificed on Days 0, 1/4, 1/2, 1,2,3,4,7, and 14. The experimental kidneys demonstrated intratubular deposits of pteroylglutamic acid with edema between Days 1 and 4 with cortical scarring by Day 14. The experimental kidneys reached maximal increases in weight (+90 per cent) on Day 2, RNA (+61 per cent, protein (+67 per cent) on Day 3, and DNA (+25 per cent) on Day 4 before falling to below control levels on Day 14. The control kidneys demonstrated the gradual incremental increases of normal renal growth throughout this period. No change in renal size, protein, RNA, or DNA could be detected in those animals who failed to demonstrate renal tubular damage. It is postulated that the response of the kidney to folic acid administration is a reparative response and not a response directed toward accelerated renal growth.

Acute Kidney Injury↗
PubMed1975

Effect of transient hydronephrosis on subsequent compensatory renal growth.

Apparent augmentation of renal growth occurs in kidneys made temporarily ischemic, or partially obstructed, before contralateral nephrectomy. The study herein was undertaken to investigate the effect of acute complete ureteral occlusion on a subsequent course of renoprival hypertrophy and hypoplasia. Three groups of animals were established. Animals in Group 1 underwent high ligation of the right ureter. Animals in Groups 2 and 3 underwent exposure and manipulation of the right ureter. Forty-eight hours later, animals in Group 1 underwent deligation and contralateral nephrectomy, animals in Group 2 underwent contralateral nephrectomy, and animals in Group 3 underwent sham operation. Animals were then selected 6 and 17 days after their second operative procedure and decapitated; the right kidneys were removed and underwent analysis with respect to wet and dry weight, total RNA, DNA, and protein content. At 6 days and at 17 days, animals in Groups 1 and 2 demonstrated no difference between these groups, although the remaining kidneys from animals in Group 1 and Group 2 were significantly larger than Group 3 animals. When compared to Group 3 animals, wet renal weight at 17 days had increased by 41 per cent, total bulk RNA had increased by 26 per cent, and total bulk DNA had increased by 33 per cent. The data support the clinical impression that transient, complete ureteral obstruction is well tolerated by the normal kidney, and that the metabolic response to obstruction does not hinder recovery after release of obstruction.

Animals↗
PubMedDate not supplied

Properties of prostatic cultures transformed by SV40.

SV-40-transformed hamster prostatic tissue has been previously evaluated as a model for human prostatic carcinoma. Because the original cell line was lost, Syrian golden hamster prostatic tissue has been established in explant culture and infected with a 10-6-cell tissue culture infectious dose (50 percent effective) of SV40. After in vitro transformation, the cells were produced in quantity and 60 times 10-6 cells were injected into adult male Syrian golden hamsters 24 hours after 400 rads of whole-body radiation. After 60-90 days, a small palpable tumor developed. These tumors could be serially transplanted in adult male animals without immunosuppression. The tumor cells were established in tissue culture and the cells were returned to adult animals without immunosuppression where they rapidly produced fast-growing tumors. The solid tumors were composed of sheets of pleomorphic polygonal cells with large nuclei and many nucleoli; they resembled undifferentiated human prostatic carcinoma. In vitro, the cultures contained small, rapidly growing cells with a population doubling time of about 1.3 days. The cells carried the SV 40-specific antigen. The modal chromosome number was 66-68 with a distribution of 47-120. Cells exposed to 2-bromo-5'-deoxyuridine in culture did not release particles with RNA-dependent DNA polymerase activity. Endocrine sensitivity in vivo and in vitro is undertermined to date.

Animals↗