Biomedical subjects

R A Britton

Publications and source records attributed to R A Britton.

At least 37 records · Page 2Linked to original sources

PubMed1992

Level of nutrition and visceral organ protein synthetic capacity and nucleic acid content in sheep.

Thirty-two crossbred wether lambs were assigned to a feed intake level of either ad libitum (ADLIB) for maximum rate of growth or restricted to maintain body weight (MAINT) throughout a 21-d period. At 7-d intervals (d 0.7, 14, and 21), four lambs per treatment were slaughtered to obtain measurements of visceral organ protein synthetic capacity and tissue composition. Protein synthetic capacity was assessed by in vitro [14C]valine incorporation and tissue RNA, DNA, and protein contents. Concentrations of protein and RNA were not significantly affected in most tissues measured. However, for liver, duodenal, and jejunal tissue, DNA concentrations in ADLIB lambs were lower (P less than .05) than in MAINT lambs. Ratios of protein:DNA in most organs were higher (P less than .05) in ADLIB than in MAINT lambs. During the 21-d period, liver and small intestinal protein and RNA mass were higher (P less than .10) in ADLIB than in MAINT lambs, and DNA mass was unaffected. Also during the 21-d period, the average total mass of ruminal protein, RNA, and DNA in ADLIB lambs was higher (P less than .05) than in MAINT lambs. Estimates of valine incorporation and ratios of RNA:protein seemed to reflect protein synthetic capacity of the visceral tissues measured; however, the effect of level of feed intake on these measurements was equivocal. These data suggest that the level of feed intake affected visceral organ mass through changes in cellular hypertrophy.

Animals↗

PubMed1992

Effect of grain sorghum hybrid on in vitro rate of starch disappearance and finishing performance of ruminants.

Forty-eight commercial grain sorghum hybrids were ranked on the basis of in vitro starch disappearance (IVSD) and starch content. Starch content ranged from 64.3 to 70.3% (P less than .01) and IVSD ranged from 5.2 to 6.3%/h (P less than .01). In the next year, 20 experimental grain sorghum hybrids consisting of 17 hybrids being developed for livestock diets and three for human diets were ranked according to IVSD, starch content, and CP content. In these samples, IVSD varied from 6.0 to 9.1%/h (P less than .05). Starch content and CP were not related to IVSD. Starch content was not correlated to CP content. Four of the original 48 grain sorghum hybrids, selected on the basis of IVSD (two fast and two slow), that differed in IVSD by 7.0% (6.5 to 7.0%/h), were fed for 133 d to steers (mean initial BW of 326 kg). The ADG of steers fed Hybrid A (fastest IVSD) was 9.0% faster than that of steers fed Hybrid D (slowest IVSD; 1.33 vs 1.22 kg, P = .06). Gain:feed ratio was positively correlated with IVSD across all treatments (R2 = .94). Hybrids A and D, another Hybrid, A1, and a bird-resistant hybrid (BR) were fed for 85 d to finishing lambs (mean initial BW of 28 kg). Lambs fed Hybrid A gained more efficiently (gain:feed) than those fed BR or A1 (.210 vs .188 and .184, P less than .05), and those fed D were intermediate (.200). Performance of sheep fed A1, with the fastest IVSD, and those fed BR, with the lowest IVSD, were similar, suggesting that factors other than IVSD affected lamb performance. Our data indicate that rate of grain sorghum starch digestion may influence feeding value of grain sorghum fed to cattle.

Animal Feed↗

PubMed1992

Plasma amino acid response to graded levels of escape protein.

A trial was conducted to examine the potential of using plasma amino acid responses to graded levels of escape protein to determine limiting amino acids in cattle. Growing calves (n = 120; mean BW = 220 +/- 21 kg) were fed a basal diet of corncob:sorghum silage (61:39) and were individually supplemented with distillers' dried grains (DDG), heat-damaged DDG (H-DDG), feather meal (FTH), or urea. The urea supplement was mixed with DDG and H-DDG to allow 0, 20, 35, 50, 65, or 80% of the supplemental CP to come from distillers' protein and maintain an 11.5% CP diet. Urea supplement was mixed with FTH to allow 0, 22, 39, 56, 73, or 90% of the supplemental CP to come from FTH. Dietary CP ranged from 11.5% at the 0% level to 17.3% at the 90% level. Plasma concentration of most essential plasma amino acids responded (P less than .10) linearly and(or) quadratically to increased escape protein. The broken-line response of plasma methionine at low DDG intake suggested that methionine was limiting at low levels of escape protein. An initial decrease followed by a plateau fit by a broken line indicated that histidine became limiting in FTH diets, and lysine eventually became limiting for DDG, H-DDG, and FTH diets before maximum BW gain was reached. Results indicate that plasma amino acid responses may identify amino acids that become limiting with increasing escape protein.

Amino Acids↗

PubMed1991

Influence of dietary forage and energy intake on metabolism and acyl-CoA synthetase activity in bovine ruminal epithelial tissue.

Twenty calves (7 mo old) were blocked by breed, sex, and weight into five groups of four calves and randomly assigned to either a 90% forage (alfalfa) or a 90% grain (50% sorghum and 50% wheat) diet fed at one (1M) or two (2M) times NEm for 140 d. Samples of ruminal epithelial tissue were collected from the anterior ventral sac, and papillae were cut free by hand and used for in vitro incubations and acyl-CoA synthetase assays. Substrates were acetate (90 mM), propionate (60 mM), butyrate (30 mM), and glucose (20 mM). Net productions of beta-hydroxybutyrate and acetoacetate from acetate were greater (P less than .01) with the 2M feeding; however, 14CO2 production from acetate was greater (P less than .05) with the grain diet. Net production of lactate (P = .09) and pyruvate (P less than .01) from propionate increased with the 2M feeding, whereas net lactate production from glucose decreased (P less than .01). Uptakes of VFA were similar with 1M and 2M feeding and were about 10-fold greater than uptakes of glucose. Production of 14CO2 from propionate was two- to fivefold greater than from acetate, butyrate, or glucose. Oxygen consumption was greater (P less than .01) with 2M feeding and unaffected by substrate. Activities of butyryl-CoA synthetase (nmol.mg of tissue-1.h-1) were greater (P less than .05) for animals consuming the forage diets. Addition of butyrate inhibited acetyl- and propionyl-CoA synthetase activity by 63 and 92%, respectively for all dietary treatments. Overall, metabolism of ruminal epithelial tissue tended to increase with the 2M feeding. Influence of dietary forage content on metabolism and activity of acyl-CoA synthetases was minimal, but the high-forage diet caused slight increases.

Animal Feed↗

PubMed1991

Compensatory growth following metabolizable protein or energy restrictions in beef steers.

One hundred sixty crossbred steers were used to compare the effects of metabolizable protein and energy restrictions on subsequent compensatory growth. Diets were formulated to impose severe or mild growth restrictions for periods of 77 to 154 d. Steers were realimented on a high-concentrate diet and fed to achieve approximately 1 cm s.c. fat thickness. Finishing performance and carcass characteristics were evaluated relative to unrestricted control animals. All restricted animals, with the exception of those on the mild, brief energy restriction, exhibited compensatory growth relative to controls. A single direct comparison of energy and protein restriction treatments indicated that finishing performance during realimentation was similar for these two groups. Evaluation of the relative effects of protein and energy restriction by multiple linear regression indicated that compensatory growth was influenced by differences in duration or severity of nutrient deprivation to a greater extent for energy-restricted than for protein-restricted steers. Linear contrasts and regression estimates indicated that compensatory growth was influenced more by differences in severity of restriction than by duration of the restriction period.

Animals↗

PubMed1991

Level of nutrition and splanchnic metabolite flux in young lambs.

Splanchnic metabolite flux was measured in young lambs given access to a high-concentrate diet either ad libitum (ADLIB) or at a maintenance level (MAINT) for 21 d. Net fluxes of urea N (UN), ammonia N (NH3 N), alpha-amino N (AAN), amino acids, glucose (G), and lactate (L) across the liver and portal-drained viscera (PDV) were measured in 11 crossbred ram lambs (35 kg) surgically fitted with indwelling catheters in the portal, hepatic, and mesenteric veins and mesenteric artery. During the 21-d period, daily N and ME intakes were 24.6 and 10.7 g N/d and 3.02 and 1.28 Mcal/d, respectively, for ADLIB and MAINT lambs. Intakes, thus, were 42% lower for MAINT than for ADLIB lambs. Net portal fluxes of UN, NH3 N, AAN, and L in MAINT lambs were 46%, 84%, 50%, and 74%, respectively, of that in ADLIB lambs. Expressed as a percentage of N intake, the proportion of AAN absorbed by the PDV was higher in MAINT lambs (P less than .05) than in ADLIB lambs. There was no net portal glucose absorption in either group of lambs; however, net hepatic glucose production in MAINT lambs was 48% of that in ADLIB lambs. There was net utilization of glutamine by the PDV; net glutamine flux in MAINT lambs was 49% of that in ADLIB lambs. The liver utilized AAN and NH3 N and produced UN. Splanchnic tissues modulate metabolite flux following changes in feed intake in young ruminants.

Amino Acids↗

PubMed1991

High-moisture corn utilization in finishing cattle.

Two finishing trials, one laboratory trial and one metabolism trial were conducted with the following objectives: 1) to determine the associative effects of feeding high-moisture corn (HMC) with either dry-rolled grain sorghum (DRGS) or dry-rolled corn (DRC) and 2) to evaluate HMC when harvested at different moisture levels, stored in different structures, or fed as whole or rolled HMC. In Trial 1, yearling steers (BW, 328 kg) were fed diets containing mixtures of HMC and DRGS. As level (0, 33, 100%, as percentage of grain DM) of DRGS increased, ADG (P less than .03) and gain/feed (P less than .001) decreased linearly; gain/feed tended to be affected quadratically (P = .14). In Trial 2, yearling steers (BW, 382 kg) fed HMC, stored whole in an upright, oxygen-limiting silo and rolled coarsely before feeding, gained faster (1.46 vs 1.36 kg/d) and more efficiently (.142 vs. .131 gain/feed) than steers fed whole HMC (P less than .01). In Trial 3, as length of storage of bunker HMC increased, in vitro rate of starch digestion and soluble N content increased (20.4 and 36.8%, respectively) and grain pH decreased (10.9%). In Trial 4, steers fed HMC or a mixture of 75% HMC with 25% DRGS had similar ruminal pH throughout a grain adaptation period, but total ruminal VFA were greater (P less than .005) for steers fed HMC alone. These data are interpreted to suggest that feeding a mixture of HMC, ground and stored in a bunker or silo bag, with DRGS will result in a 3.2% associative effect. However, no associative effects were measured when a mixture of HMC, stored whole and fed whole or rolled, and DRC were fed.

Animal Feed↗

PubMed1991

Escape protein for beef cows: III. Performance of lactating beef cows grazing smooth brome or big bluestem.

Grazing trials were conducted utilizing individually supplemented lactating beef cows in a 2-yr study to determine the effect of supplemental escape protein (EP) on cow/calf performance during the active growth periods of smooth brome and big bluestem. Graded levels (0, .11, .23, and .34 kg of EP/animal from an equal-protein-basis mixture of blood meal and corn gluten meal were offered daily, replacing corn starch and molasses, which was used as the energy control. All cows received 582 g of supplemental DM/d. Analyses for the smooth brome study indicated a cubic response (P less than .05) to milk production and calf daily gain. No response to EP supplementation (P greater than .10) was observed for the big bluestem study. Analysis of esophageal extrusa samples collected throughout the grazing seasons for both years demonstrated that quality of forage selected by the animal decreased as the trial progressed. Using a 16-h modified dacron bag technique that did not adjust for microbial attachment, protein escaping degradation for smooth brome and big bluestem was estimated to range from 7.0 to 14.5% and 22.2 to 30.3%, respectively. There was limited response to a moderate level of EP supplementation of lactating cows grazing smooth brome, but calf gains were depressed by both the low and the high levels of EP. Lactating cows grazing big bluestem did not respond to EP supplementation, but added energy supplement depressed calf gains.

Animal Feed↗

PubMed1991

Growth, body composition, and visceral organ mass and metabolism in lambs during and after metabolizable protein or net energy restrictions.

Three trials were conducted to assess effects of metabolizable protein and NE deficiencies on changes in body composition, organ mass and metabolism, and animal growth performance during restriction and realimentation. Growth of lambs was restricted to achieve no change in BW for periods of 5 to 6 wk by limiting intake of metabolizable protein or NE. In Trial 1, changes in body composition and visceral organ mass and metabolism during restriction were compared to unrestricted controls using 36 lambs. Trial 2 was designed to investigate changes in growth, body composition, and visceral organs during restriction and realimentation periods using 44 lambs. Trial 3 was limited to evaluation of differences in performance and carcass characteristics of previously restricted and unrestricted ram lambs (15 total). Results of Trial 1 indicated that liver weights were decreased with nutrient restrictions. Body protein mass was conserved in energy-restricted (ER) lambs and lost in protein-restricted (PR) lambs. Fat was mobilized at similar rates for PR and ER lambs. In Trial 2, liver and intestinal weights, as well as in vitro oxygen consumption by liver slices, were decreased with nutrient restrictions. The reductions persisted after 2 wk of realimentation, yet no compensatory growth was observed. Feed intakes were increased gradually during the first 2 wk of realimentation. Composition of gain during the realimentation period was similar to that of unrestricted lambs. In Trial 3, neither gain nor feed efficiency during realimentation was enhanced as a result of previous nutrient deficiencies. Absence of compensatory growth in Trial 3 is possibly attributable to differences in gastrointestinal fill. Lambs subjected to short-term PR and ER seem to have similar recuperative capacity.

Adipose Tissue↗

PubMed1991

Type and mixtures of high-moisture corn in beef cattle finishing diets.

In three experiments consisting of three finishing trials each, five corn storage and(or) feeding treatments were evaluated: 1) dry whole (DWC); 2) whole high-moisture (WHMC); 3) mixture (MHMC) of ground (GHMC) or rolled (RHMC) high-moisture corn with WHMC; 4) GHMC stored in a bunker silo; and 5) RHMC, corn stored whole but fed in rolled form. In Exp. 1, gains and feed intakes of steers fed whole corn (DWC vs WHMC) were similar. Steers fed GHMC and RHMC had lower (P less than .05) gains and intakes than steers fed whole corn. However, feed to gain ratios were 9% better (P less than .10) for steers fed RHMC than for steers fed GHMC. In Exp. 1 and 2, gains of steers fed MHMC were intermediate to gains of steers fed whole (DWC and WHMC) or processed corn (GHMC and RHMC). Feed to gain ratios of steers fed whole or processed corn were similar to feed to gain ratios of steers fed MHMC. In Exp. 3, steers fed 28.6% moisture GHMC had lower (P less than .05) intakes and feed to gain ratios than steers fed 22.5% moisture GHMC. Intakes and feed to gain ratios decreased 1.2 and 1.4%, respectively, for each 1% increase in corn moisture. Data are interpreted to mean that the relative effect of corn moisture content on cattle performance depends on form of corn fed; positive associative effects of MHMC are negligible, but RHMC has a greater feed value than GHMC.

Animal Feed↗

PubMed1990

Level of nutrition and visceral organ size and metabolic activity in sheep.

Thirty-two crossbred wether lambs (initial live-weight 31 kg) were fed on a diet (metabolizable energy (ME) 12.8 MJ/kg) ad lib. (ADLIB) or restricted to maintain body-weight (MAINT) for a 21 d period. On days 0, 7, 14 and 21, four lambs per treatment were slaughtered, visceral organs weighed and tissues sampled. During the 21 d period, ME intake in ADLIB lambs increased quadratically with an average rate of live-weight gain of 425 g/d. In MAINT lambs, live weight (30 kg) was maintained, and daily ME intake (kJ/kg empty body-weight (EBW)0.75) declined (P less than 0.01) quadratically with time. Weights of liver, stomach and small intestines as a percentage of EBW were increased in ADLIB lambs and decreased by 10-33% in MAINT lambs (treatment x day, P less than 0.01). In vitro liver oxygen consumption was not affected by level of feed intake. Estimates of whole-liver O2 consumption (mmol O2/d per kg EBW) increased in ADLIB lambs and were relatively constant in MAINT lambs. These findings suggest that level of feed intake changes the relative proportion of visceral organs to body mass. In addition, the effect of level of feed intake on changes in the relative contribution of visceral organs to whole-body metabolic rate appears to be primarily a result of differences in organ size rather than tissue-specific metabolic activity.

Animal Nutritional Physiological Phenomena↗

PubMed1990

Protein value of feather meal for ruminants as affected by blood additions.

In situ digestion and growth studies were conducted to determine the effects of adding blood to feather meal. In the in situ and digestion studies, soybean meal (SBM), blood meal (BM), feather meal (Fth) and two combinations of 55% raw blood and 45% feathers (RB + F) were used. Feathers and blood were combined either before or after steam hydrolysis of feathers. Ruminal escape protein of Fth was greater than that of SBM but less than that of BM (P less than .10) determined in situ after 12 h of incubation. Ruminal in situ protein digestion was lower (P less than .10) than for the other treatments (P less than .10) when blood was hydrolyzed with feathers. Total tract digestibility was similar (P greater than .10) for SBM, BM and the mixture of RB + F when the blood was not hydrolyzed. In the growth study, calves were supplemented with urea, SBM, BM, Fth or a combination of BM and Fth (BM + Fth; each supplied an equal portion of supplemental protein). The slope ratio technique was used to evaluate the protein sources. The most efficiently used protein sources were BM and BM + Fth compared to SBM and Fth (P less than .05). There was a numerical but not significant (P greater than .1) complementary effect of adding BM to Fth. Soybean meal and Fth had similar protein efficiencies (P greater than .20). Estimated amino acid flow to the small intestine based on in situ amino acid degradation suggested that the complementary effect observed for BM + Fth was due to BM supplying lysine and Fth providing sulfur amino acids.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acids↗

PubMed1990

Hydrolyzed feather meal as a protein source for growing calves.

Growth, digestion and in situ studies were conducted to determine the protein value of hydrolyzed feather meal (Fth) for growing ruminants. Dacron bags containing blood meal (BM), Fth, corn gluten meal (CGM) and soybean meal (SBM) were suspended in the rumen of two steers for 12 h to estimate escape protein. The escape protein value for Fth, 69.1%, was less than that for BM (82.8%) and CGM (80.4%; P less than .05) but greater than that for SBM (26.6%; P less than .05). Apparent protein digestion by lambs was similar (P greater than .10) for isonitrogenous diets containing urea (U), BM, Fth, CGM and SBM. Amino acid contents of the protein sources before vs after a 12-h ruminal in situ digestion were similar (P greater than .10). In a growth study, a basal diet of 80% ensiled corncobs and 20% alfalfa was fed to 60 individually fed crossbred steers (215 kg BW). Steers were supplemented with U, BM, Fth, 1/2 BM:1/2 Fth, 1/2 BM:1/2 CGM and 1/3 BM:1/3 Fth:1/3 CGM (protein basis). Protein sources were fed at 30, 45 and 60% of the supplemental N with urea supplying the remainder. Protein efficiency was calculated using the slope ratio technique. Protein efficiency was similar (P greater than .10) for BM- and Fth-supplemented calves. Protein efficiencies were similar (P greater than .10) for BM:CGM, BM:Fth and BM:Fth:CGM combinations. These data indicate the Fth is a digestible high escape protein source that is useful in diets for growing ruminants.

Amino Acids↗

PubMed1989

Effect of level of nutrition on splanchnic blood flow and oxygen consumption in sheep.

The objective of the present study was to measure changes in splanchnic blood flow and oxygen consumption in sheep fed on a high-concentrate diet ad lib. (ADLIB) or an amount sufficient to maintain body-weight (MAINT) for 21 d. Eleven ram lambs were surgically implanted with chronic indwelling catheters in the portal, hepatic and mesenteric veins and mesenteric artery to measure blood flow and net O2 flux through the liver and portal-drained viscera (PDV). During the 21 d period, PDV (P less than 0.05) and liver (P less than 0.01) blood flow increased in ADLIB and decreased in MAINT lambs (treatment x day, linear). After 21 d, O2 consumptions in PDV and liver of MAINT lambs were 37 and 63% lower than in ADLIB lambs. In the control period, total splanchnic tissues represented an average of 52% of whole body O2 consumption. After 21 d, the relative contributions of PDV and liver to whole-body O2 consumption were 28 and 41% in ADLIB and 19 and 22% in MAINT lambs respectively. Allometric regression variables indicate that liver O2 consumption responds more rapidly to changes in metabolizable energy intake than portal O2 consumption. These results indicate that blood flow and O2 consumption in both PDV and liver are related to level of nutrition. Furthermore, splanchnic tissues represent a significant component of whole-body O2 consumption that is subject to manipulation by level of nutrition.

Animal Nutritional Physiological Phenomena↗

PubMed1989

Growth response to escape protein and forage intake by growing cattle grazing cornstalks.

Two trials were conducted to study the protein requirements of growing cattle grazing cornstalks. Diet composition and forage intake were determined at different periods during the grazing season. Residue intake and dietary CP content decreased (P less than .05) .079 kg and .044 percentage units per day of grazing, respectively. In vitro DM disappearance of the roughage fraction of the diet remained high early in the grazing period when husk availability was high, then declined in a curvilinear pattern. Calves required time to learn to find and consume the residual grain. During 54 d of grazing in Trial 1, calves supplemented with 409 g CP gained .105 kg/d more than those supplemented with 213 g CP. Maximal daily gain (.308 kg) in Trial 2 was obtained with 163 g/d of ruminal escape protein. Growing cattle grazing cornstalks responded to protein supplementation at levels above current National Research Council recommendations for 250-kg calves gaining .3 kg/d.

Animal Feed↗

PubMed1988

Visceral organ size and hepatocyte metabolic activity in fed and fasted rats.

Changes in visceral organ mass and hepatocyte metabolic activity in response to nutrient deprivation were studied in male Sprague-Dawley rats. Forty-two rats (320 g) were given ad libitum access to feed or fasted for 72 h after which time visceral organ mass and metabolic activity were measured. Liver metabolic activity was measured in vitro by [14C]valine incorporation into acid-precipitable protein and oxygen consumption in isolated hepatocytes. Fasted rats had lower weights of liver and intestines and similar kidney and stomach weights relative to body weight than fed rats. Compared with fed controls, fasted rats had lower RNA and higher DNA concentrations in liver and intestines with decreased RNA and protein mass in all visceral organs. Nutrient deprivation generally resulted in reduced ratios of RNA/DNA and protein/DNA in visceral tissues. Nutrient deprivation had no effect on in vitro oxygen consumption or [14C]valine incorporation expressed per g tissue. However, in vitro oxygen consumption and valine incorporation expressed per unit DNA and estimated total liver oxygen consumption were reduced in fasted rats. These data suggest that nutrient deprivation reduced visceral organ mass of RNA and protein primarily through an apparent reduction in cell size. Nutrient deprivation resulted in decreased liver oxygen consumption because of decreased organ size rather than by tissue metabolic activity.

Animals↗

PubMed1988

Monensin level during grain adaptation and finishing performance in cattle.

Two finishing trials were conducted to measure the response of cattle adjusting to high-concentrate diets to dietary monensin level. In trial 1, 54 individually fed Hereford-Angus steers (312 kg), previously fed a two-thirds corn silage: one-third corncob-based diet, were allotted in a 2 X 3 factorial arrangement of treatment with tylosin (0, 11 mg/kg) and monesin (0, 11, 33 mg/kg) fed during a 28-d, grain-adaptation period (fed 75% concentrate for 6 d and then fed 95% concentrate). After 28 d, all steers were continued on their respective levels of tylosin and 33 mg/kg monensin for the remaining 119 d. Daily intake patterns indicated digestive upset in all treatments during adjustment to the 95%-concentrate diet. Blood samples taken during the first 28 d revealed no differences in acid-base status in response to monensin level; however, all steers exhibited reduced (P less than .01) pH and HCO3 and increased (P less than .01) lactate after 4 d on a 75%-concentrate diet. In the initial 28 d, intake decreased (linear P less than .05) as the level of monensin increased. In the total finishing period, however, increasing the level of monensin fed during grain adaptation decreased (linear P less than .05) intake and tended to decrease (linear P = .20) gain with no effect on feed efficiency.(ABSTRACT TRUNCATED AT 250 WORDS)

Acidosis↗

PubMed1987

Induced non-enzymatic browning of soybean meal. I. Effects of factors controlling non-enzymatic browning on in vitro ammonia release.

Non-enzymatic browning was tested as a means of suppressing degradation of soybean meal (SBM) by ruminal microbes in five trials with in vitro ammonia release as the response criterion. Treatments imposed on SBM included reducing sugar source (xylose, glucose, fructose and lactose), reducing sugar level (1, 3 and 5 mol/mol SBM-lysine), pH (6.5, 8.5 and 10.0), dry matter (DM) content (65, 70, 75, 80, 85 and 90%) and varying lengths of heating time (0 to 90 min) at 150 C. Samples heated under conditions that promoted non-enzymatic browning gave greater (P less than .01) ammonia release suppression that when SBM was heated without these treatments. Xylose was the most reactive sugar, but extended heating of SBM containing glucose, fructose or lactose resulted in ammonia release similar to xylose. Increasing sugar level from 1 to 5 mol/mol SBM-lysine caused linear decreases (P less than .01) in ammonia release for xylose, glucose and fructose, but not lactose. Ammonia release was higher (P less than .01) at pH 6.5 than pH 8.5 and 10.0, and higher (P less than .01) at pH 9.5 than pH 10.0. Rate of non-enzymatic browning decreased when samples containing greater than 80% DM were heated. These results are interpreted to show that controlled non-enzymatic browning may be effective for reducing ruminal degradation of SBM.

Amines↗