PubMed Health⌕ Search

Biomedical subjects

R A Curry

Publications and source records attributed to R A Curry.

11 recordsLinked to original sources
PubMed1993

Monoclonal antibodies to lipopolysaccharide of four oral bacteria associated with periodontal disease.

Periodontal disease is a common inflammatory disease which erodes the supporting structures of the teeth, and is initiated by a subgingival infection with selected Gram-negative bacteria. Monoclonal antibodies (mAb) to lipopolysaccharide (LPS) of four periodontal pathogens, A. actinomycetemcomitans, P. intermedia, F. nucleatum and P. gingivalis were examined for specificity and their ability to bind these pathogens in a particle concentration fluorescence immunoassay (PCFIA). The mAb selected were specific for their homologous bacteria and when tested against a large battery of other bacteria, including 16 genera and 46 species, were found not to cross-react with heterologous species. When each of the mAb was challenged with 40 or more homologous freshly isolated bacteria, more than 90% were positive. Non-cellular antigens in the form of soluble LPS and extracellular vesicles were examined for their ability to bind to assay components and alter the apparent results of the assay. LPS was found to have potential as an interfering agent if bound to assay components prior to sample treatment, but this non-specific binding was significantly reduced when a surfactant was added to the buffers. Extracellular vesicles had no significant effect on the estimation of P. gingivalis by the assay.

Aggregatibacter actinomycetemcomitans↗
PubMed1989

Neurosonology--who should perform the study?

Neurosonology is a rapidly growing field of imaging which demands a highly skilled imaging professional. Sonographers are imagers trained to produce sonographic images of one or more of the following specialty areas: abdomen, obstetrics/gynecology, adult echocardiography, pediatric echocardiography, opthalmology, vascular technology, and neurosonology. There are short and long term training programs for sonographers which vary in scope and the number of specialty areas offered. Some sonographers do not receive formal training and learn OTJ (on-the-job). Any training program in neurosonology should encompass anatomy, basic pathology, sonographic appearance of normal and abnormal structures, and physics/instrumentation. There is presently a sonographer shortage due to the rapid increase in sonographer positions nationwide. It is therefore beneficial to the neurosonology department to allow ample time to recruit and/or train a sonographer. The Society of Diagnostic Medical Sonographers, the American Registry of Diagnostic Medical Sonographers, and the American Institute of Ultrasound in Medicine are valuable resources in providing information on training opportunities and recruitment strategies for sonographers.

Humans↗
PubMed1984

Inhibition of platelet aggregation by monoclonal antibody reactive with beta 2-microglobulin chain of HLA complex.

A mouse monoclonal antibody that reacts with beta 2-microglobulin, the light chain of class I major histocompatibility antigens, inhibited the second wave of human platelet aggregation induced by adenosine diphosphate and epinephrine and blocked aggregation and platelet protein phosphorylation induced by sodium arachidonate. Thrombin-induced platelet aggregation was inhibited at threshold concentrations but not at higher concentrations. The antibody also inhibited aggregation and secretion in response to thromboxane A2 or the stable endoperoxide analog, U46619. These results suggest that beta 2-microglobulin in the histocompatibility complex is intimately associated with transmission of the endoperoxide-thromboxane signal at the platelet membrane.

15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5↗
PubMed1981

Discordant expression of 2 Epstein-Barr virus-associated antigens, EBNA and RANA, in man-rodent somatic cell hybrids.

The Epstein Barr nuclear antigen (EBNA) and the rheumatoid arthritis nuclear antigen (RANA) develop in human B lymphocytes that have been infected and transformed by Epstein Barr virus (EBV). Antibodies to RANA and EBNA are found only in individuals with prior exposure to EBV. The purpose of the present studies was to determine the relation of the 2 antigens to each other and to EBV genetic material, in human-rodent somatic cell hybrids. Cultured human B lymphoblastoid cells, Raji, Daudi, and RPMI 4098 were fused with thymidine kinase-deficient mouse or hamster fibroblasts. After selection and cloning in ouabain-HAT medium, the hybrid nature of the surviving cells was confirmed by isozyme analysis. The hybrid clones were analyzed for EBNA by anti-complement im,munofluorescence, and for RANA by anti-immunoglobulin immunofluorescence and immunodiffusion. The results showed that RANA and EBNA segregated entirely independently of each other in the hybrid clones. Two methods were used to detect the presence of EBV DNA sequences in the intracellular DNA of hybrid clones. The 1st method relied on the hybridization of labeled cRNA prepared from virion DNA with DNA from 8 hybrid clones affixed to nitrocellulose filters. The 2nd approach was to hybridize labeled intracellular DNA from 3 hybrid clones to Southern blots of cloned fragments of EBV DNA. These results suggested that the presence of EBV DNA was not sufficient for the expression of either antigen. One stable RANA-positive hybrid cell line contained at least 80% of the EBV genome in the absence of detectable EBNA.

Animals↗
PubMed1981

Lysis of cultured human melanoma M10 cells by polyclonal xenoantibodies to melanoma-associated antigens.

Cultured human melanoma cells M10 harvested from cultures in different phases of their growth show significant changes in the expression of melanoma-associated antigens (MAA), but they do not vary in susceptibility to lysis mediated by anti-MAA xenoantisera and effected by complement or lymphoid cells. Furthermore, melanoma cells M10 showed a significant increase in susceptibility to immune lysis following treatment with puromycin at doses that do not effect the expression of MAA. The lack of correlation between MAA density and susceptibility to immune lysis supports the contention that, under the experimental conditions used, cellular properties play a major role in the outcome of immune attack.

Antibodies, Neoplasm↗
PubMed1980

A novel molecule expressing HLA-DR antigenic determinants.

Our previous studies suggested that the polymorphism of HLA-DR antigens (the human equivalent of murine I-E antigens) was a result of structural variation in the small (beta) subunit. In order to more accurately define this polymorphism we have expanded these studies to include HLA-DR antigens isolated with monoclonal cells derived from genotypically HLA-homozygous DRw2, DR2w5, and DRw7 lymphoblastoid cells derived from offspring of consanguineous relationships. Our results indicate the large (alpha) subunits of DRw2 and DRw7 antigens are nearly identical, while their beta subunits show many differences. In contrast, both the alpha and beta subunits of the DRw5 antigen differ strikingly from the respective subunits of the DRw2 and DRw7 antigens. The significance of the variability of the DRw5 alpha subunit is in question at this point. One intriguing possibility is that DRw5 actually represents the human counterpart of the mouse I-A subregion antigen and that the monoclonal antibody is reacting with a determinant which is shared by the human equivalents of murine I-A and I-E antigens.

Amino Acid Sequence↗
PubMed1980

Appearance of receptors for Macaca speciosa red blood cells on human fibroblasts transformed by simian virus 40.

Receptors for monkey red blood cells (MRBC) that are expressed on subpopulations of human lymphoid cells are coded by genes linked to the major histocompatibility complex (MHC) region. Since malignant transformation of cells is associated with changes in structures coded by the MHC region, 10 cultured human melanoma and sarcoma cells and autologous SV40-transformed fibroblasts were tested for expression of MRBC receptors and compared with normal autologous fibroblasts. Only 2 of the tumor cell lines and normal fibroblasts from the same individual formed rosettes with MRBC. On the other hand, SV40 transformation induced in all the fibroblasts expression of receptors for MRBC. MRBC receptors on SV40-transformed fibroblasts show properties similar to those on B lymphoid cells.

Animals↗