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R A Holt

Publications and source records attributed to R A Holt.

23 records · Page 2Linked to original sources
PubMed1984

Production of Solvents by Clostridium acetobutylicum Cultures Maintained at Neutral pH.

The formation of acetone and n-butanol by Clostridium acetobutylicum NCIB 8052 (ATCC 824) was monitored in batch culture at 35 degrees C in a glucose (2% [wt/vol]) minimal medium maintained throughout at either pH 5.0 or 7.0. At pH 5, good solvent production was obtained in the unsupplemented medium, although addition of acetate plus butyrate (10 mM each) caused solvent production to be initiated at a lower biomass concentration. At pH 7, although a purely acidogenic fermentation was maintained in the unsupplemented medium, low concentrations of acetone and n-butanol were produced when the glucose content of the medium was increased (to 4% [wt/vol]). Substantial solvent concentrations were, however, obtained at pH 7 in the 2% glucose medium supplemented with high concentrations of acetate plus butyrate (100 mM each, supplied as their potassium salts). Thus, C. acetobutylicum NCIB 8052, like C. beijerinckii VPI 13436, is able to produce solvents at neutral pH, although good yields are obtained only when adequately high concentrations of acetate and butyrate are supplied. Supplementation of the glucose minimal medium with propionate (20 mM) at pH 5 led to the production of some n-propanol as well as acetone and n-butanol; the final culture medium was virtually acid free. At pH 7, supplementation with propionate (150 mM) again led to the formation of n-propanol but also provoked production of some acetone and n-butanol, although in considerably smaller amounts than were obtained when the same basal medium had been fortified with acetate and butyrate at pH 7.

Journal Article↗
PubMed1981

A hollow complete lower denture.

A technique was described that greatly reduces the weight of an exceptionally heavy lower denture by as much as 25%. When weight of a denture may be a contributing factor to the successful resolution of a patient's problem, the hollow denture should be considered.

Denture Design↗
PubMedDate not supplied

Force versus time profiles of selected heat-processed denture liners.

The potential correlation of the force versus time behavior of four resilient lining materials and their acceptance by patients was investigated. Two modified acrylic resins and two silicone denture lining materials were tested. The response of the soft lining materials to a programmed indentation test was used to define a parameter for each denture liner product. A parameter defined as the "Rebound Index," using force-time data obtained from the indentation measurements, was significantly different for the modified acrylic resins compared to the silicone materials. Improved patient acceptance was correlated with higher values of this index.

Acrylic Resins↗